57 results about "Quasipaa spinosa" patented technology

The invention relates to the field of aquaculture, in particular to a rana spinosa breeding method, which comprises seed selection, spawning, maintaining of tadpole and breeding of froglets and adult frogs. The breeding of froglets and adult frogs comprises construction of breeding ponds, feeding of baits, daily management and regular epidemic prevention. A froglet breeding pond and an adult frog breeding pond are constructed for respective breeding management of the froglets and the adult frogs; feeding is performed with the fixed time, fixed location, fixed quantity and fixed quality to ensure comprehensive nutrients and rapid growth of frogs and reduce diseases of the frogs; an insulation device is set in each breeding pond to increase the temperature of water in the ponds so that frogs can continuously move and eat food without winter sleep, growth of the frogs can be accelerated, and the breeding cycle can be shortened; and the regular epidemic prevention is performed for the breeding ponds with traditional Chinese medicine and disinfectants to prevent diseases of the frogs. By adopting the breeding method, better economic benefits can be achieved, the spawning rate of female frogs is 91.7 percent, the egg fertilization rate is 93 percent, the hatching rate is 96.6 percent, the tadpole metamorphosis rate is 88.4 percent, the froglet survival rate is 90.3 percent, and the survival rate of adult frogs is 93.9 percent after breeding.

A giant spiny frog ecological breeding method includes the following steps of water conditioning, breeding frog breeding, breeding frog oviposition, frogspawn hatching, tadpole breeding, tadpole metamorphosis, froglet breeding and adult frog raising, so that adult frogs are obtained. The giant spiny frog ecological breeding method has the advantages that giant spiny frogs grow rapidly and healthily, are robust and reach the growth level and final quality of wild giant spiny frogs, the growth survival rate of giant spiny frogs is increased, influences caused by diseases on growth of giant spiny frogs are reduced by the adoption of disease prevention measures, the survival rate and quality of giant spiny frogs are improved, giant spiny frogs have high survivability and disease resistance, giant spiny frogs grow healthily and robustly by the adoption of a nutrition equilibrium mode during raising, produced giant spiny frogs have a short growth cycle under the ecological breeding environment and have good output quality, market supply can be increased by produced giant spiny frogs, market prospects are wide, and popularization of giant spiny frog breeding in Longquan mountain and forest regions is facilitated.

The invention discloses a bionic cultivation method for a quasipaa spinosa. The bionic cultivation method for the quasipaa spinosa includes steps of selecting a site, constructing a quasipaa spinosa pond, hatching and seedling, cultivating to obtain an adult quasipaa spinosa and feeding fodder. Because the bionic cultivation method for the quasipaa spinosa uses the bionic cultivation method, the disease resistance of the quasipaa spinosa is strong, the survival rate is high, the nutrition is balanced, the quality is high, the problem of gradual exhaustion of wild quasipaa spinosa resource due to hunting and living environment destruction is well solved, and a good way is developed for protecting and expanding the wild quasipaa spinosa resource.

The invention belongs to the field of aquaculture, and particularly relates to a cultivation method of quasipaa spinosa tadpole metamorphosis larvae. The method comprises a tadpole cultivation pool, ambient conditions, stocking of small tadpoles, a culture method and a method for promoting metamorphosis. The illumination intensity suitable for a tadpole breeding stage, the illumination intensity suitable for a tadpole metamorphosis stage and the illumination intensity for controlling the metamorphosis speed are optimized, so that according to the growth stage and the metamorphosis stage of tadpoles, characteristic nutrition is optimized, and a water quality control method of combination of quicklime water and slow flowing water is optimized; for the tadpoles which are hatched in spring, conditions of illumination, water temperature, nutrition and the like are optimized, so as to shorten the growing period of the tadpoles; for the tadpoles which are hatched in autumn, corresponding conditions of illumination, water temperature, nutrition and the like are also optimized, so as to extend the growing period of the tadpoles; the growing development and metamorphosis development of the tadpoles are promoted, and diseases can be prevented, thereby increasing the survival rate and metamorphosis rate of the tadpoles.

The invention discloses quasipaa spinosa tadpole feed and a preparation method thereof. The quasipaa spinosa tadpole feed is prepared from fish meal, earthworm powder, insect protein mixed powder, corn flour, wheat bran powder, bean pulp, rapeseed dregs, strong flour, alpha-starch, wheat flour, enteromorpha seaweed meal, mulberry leaf powder, yeast powder, fish oil, soybean oil, phosphatide oil, 50-percent choline chloride, beta-1,3 glucan, mineral substance premixes, vitamin premixes, lactic acid bacteria and bacillus subtilis through multiple times of mixing and smashing. The feed has the advantages that used raw material sources are stable; a formula is reasonable; a production process is simple and feasible; the whole nutritional requirements of the high-density cultivation of quasipaa spinosa tadpoles can be met; the cost is relatively low; the problems of bait storage difficulty, germ breeding and the like in the cultivation process can be effectively solved; the quality is safe and reliable; the tadpoles can fast grow; the death rate is low; the metamorphosis time is early; the feed conversion rate is high; the cultivation benefits are better.

The invention provides a preparation method of quasipaa spinosa active peptide nutrition powder. The quasipaa spinosa active peptide nutrition powder comprises the following raw materials of quasipaaspinosa peptide powder and fruit powder. The preparation method of the quasipaa spinosa active peptide nutrition powder comprises the following steps of freezing sterilization, fishy flavor removal, grinding into thick liquid, enzymolysis, odor removal, spray drying and preparation of a finished product, wherein quasipaa spinosa is prepared into the peptide powder, and the peptide powder and the fruit powder are compounded to prepare the quasipaa spinosa active peptide nutrition powder. The active peptide nutrition powder comprises 16 amino acids and 20 trace elements or above, has high nutrition content, is rich in mineral substances, promotes osteocytogenesis, rapidly supplements calcium, can be directly absorbed by the intestinal tract, rapidly supplements active cells, increases antibodies, promotes the immune function and metabolism, is simple and convenient to eat, and can be directly drunk after being mixed with warm water or eaten after being stirred with supplementary food.

The invention discloses a high-efficient environmentally-friendly feed for quasipaa spinosa. The high-efficient environmentally-friendly feed is made from the following raw materials and components in percentage by mass: 20-25% of fish meal, 2-10% of chicken meat powder, 1-6% of porcine blood corpuscle protein powder, 1-5% of dried earthworm powder, 2-5% of dried fly larva powder, 10-30% of soybean meal, 18-28% of flour, 1-3% of soybean lecithin, 2-6% of fish oil, 0.6-0.8% of composite vitamins, 1-2% of composite mineral substances, 1-3% of calcium dihydrogen phosphate, 0.1-0.4% of choline chloride, 0.01-0.04% of betaine, 0.01-0.05% of L-carnitine, 0.01-0.04% of bile acid and 0.01-0.02% of phytase. The invention further discloses a preparation method of the feed. According to the feed for the quasipaa spinosa disclosed by the invention, the fermented soybean meal and the phytase are added, so that the coefficient of the feed is reduced; the dosage of the dried earthworm powder, the dosage the dried fly larva powder and the dosage of the betaine are adopted, so that the feed has excellent food-calling effects on the quasipaa spinosa, after being domesticated and raised for a long term, the quasipaa spinosa can be completely adapted to feeding of artificial compound feeds, the water resistance is good, breeding water bodies are not polluted, and the survival rate is increased.

The invention provides an ecological breeding system of giant spiny frogs. The system comprises a water storage pool, a frog spawn incubation area, a tadpole feeding area, a giant spiny frog feeding area and a bait feeding area, wherein a water inlet and a water outlet are formed in each of the water storage pool, the frog spawn incubation area, the tadpole feeding area, the giant spiny frog feeding area and the bait feeding area; the water outlet of the water storage pool is connected with the water inlets of the frog spawn incubation area, the tadpole feeding area and the giant spiny frog feeding area respectively; the water inlet of the bait feeding area is connected with the water outlets of the frog spawn incubation area, the tadpole feeding area and the giant spiny frog feeding arearespectively; filter facilities are arranged at the water inlet of the water storage pool and the water outlet of the bait feeding area. The ecological breeding system is established by simulating thenatural growth environment of the giant spiny frogs; the system can directly utilize mountain spring water or stream as a water source, breeding water quality is convenient to control by means of thewater storage pool, a bait feeding pool is arranged, so that on one hand, baits for breeding the giant spiny frogs are raised, on the other hand, the water is purified, drained water cannot pollute the environment, and meanwhile, the problems of low survival rate, high probability of infection with diseases and the like in the breeding process are solved.

The invention discloses a combined ecological breeding system for giant spiny frogs. The system comprises a breeding frog spawning area used for breeding frogs to live and breed, a hatching area usedfor frog spawns to be hatched, a growing area used for feeding froglets, a water supplying channel used for separately supplying flowing water to the breeding frog spawning area, the hatching area andthe growing area, and a fishpond for collecting the flowing water used by the breeding frog spawning area, the hatching area and the growing area. The combined ecological breeding system for giant spiny frogs is good in structural design and is a combined ecological breeding mode combining the indoor environment with the outdoor environment, the whole breeding system is complete in ecological construction, all steps are strictly controlled, and especially the breeding frog spawning step and the spawn hatching step are treated well so that the breeding frogs cannot be easily infected externally and get ill; the hatching rate of frog spawns is high, the life habits of the giant spiny frogs can be met, the growing speed is high, therefore the yield of the giant spiny frogs is increased, thewhole ecological construction cost is low, and the system is beneficial for using and popularization.

The invention discloses a Quasipaa spinosa tadpole breeding method and relates to the technical field of Quasipaa spinosa breeding. The method is characterized by comprising the steps of early-stage breeding, wherein feeding is conducted after yolk sacs on the abdomens of newly hatched tadpoles disappear 2-3 days after hatching, cooked egg yokes and soybean milk can serve as initial feed, one egg yoke is fed to 4000 tadpoles each time, and feeding is conducted twice a day; mid-stage breeding, wherein compound feed can be fed 5-7 days later, feeding amount is increased along with the growth of the tadpoles, bait can be directly spread on water, and in general, 800-1000 tadpoles which are 10 days old can be put on each square meter of water for breeding, and 600-800 tadpoles which are 15 days old can be put on each square meter of water for breeding; metamorphosis-period breeding, wherein special metamorphosis-period feed is adopted, and metamorphosis is completed after the tadpoles are fed for 70 days. According to the method, nutrition preparation is reasonable and comprehensive, growth of Quasipaa spinosa tadpoles is facilitated, oxygen can be supplemented, the PH of water can be controlled, the survival rate of tadpoles can reach over 85%, and economic benefits are increased.

The invention discloses a method for hatching and breeding of quasipaa spinosa. The method is characterized by comprising the following steps: (1) hatching preparation: sterilizing a hatching room byusing 75% ethanol and a sodium hypochlorite solution, cleaning and then putting quasipaa spinosa spawns into hatching troughs filled with sterilizing fountain; (2) hatching: keeping the quantity of quasipaa spinosa spawns in each hatching trough at 200-2300, putting the hatching troughs into a thermostat water bath for hatching, regulating the temperature and the illumination intensity so as to hatch the quasipaa spinosa spawns; (3) metamorphosis breeding: putting hatched quasipaa spinosa tadpoles into a tadpole breeding pool in a breeding room for breeding, feeding the quasipaa spinosa tadpoles by mixing of protein powder and eggs in the early 7 days after being put into the breeding pool, then gradually increasing the fodder feeding amount, and adding an extraction solution to the breeding pool once every two days, until the metamorphosis phase is ended; and (4) domestication breeding: putting the young quasipaa spinosa into the breeding pool, and performing domestication breeding inthe condition of water temperature of 15-18 DEG C.

The invention discloses rana spinosa tadpole feed. The rana spinosa tadpole feed comprises artificial compound feed, vegetable pumpkin feed and mixed feed, wherein the mixed feed comprises the artificial compound feed and the vegetable pumpkin feed according to a weight ratio of 1: 1. After adoption of the rana spinosa tadpole feed, tadpoles accumulate more nutrition, and metamorphose into frogs with larger body shapes, so that the survival potential can be further improved and the survival rate can be increased; the tadpoles are omnivorous, and full compound feed meets food habits of the tadpoles and nutrients needed during growth, so that improvement on the disease resistance of the tadpoles is facilitated, and the tadpoles are high growth speed, long in total length and high in metamorphosis survival rate; through increase in the metamorphosis rate and the disease resistance, the growth cycle of the tadpoles is shortened to about 90 days, and the growth cycle is short. The rana spinosa tadpole feed and the feeding method thereof have the characteristics of capability of greatly increasing the survival rate, the total length and the metamorphosis survival rate of the tadpoles and great reduction in the feeding cost, and have the advantages of a short feeding cycle and strong disease resistance of the tadpoles.

The invention provides a giant spiny frog inducing spawning method which comprises the steps that in April, when air temperature is above 20 DEG C, giant spiny frog parents with the female-male ratio of 1 : 1 are placed in a reproduction frog pool for hybridization and spawning, the released density is 1-2 pairs per square meter, the reproduction frog pool is arranged in a reproduction frog chamber, indoor illumination intensity is controlled to be 2000-5000 lux, natural illumination time is kept, pool water temperature is kept at 19-20 DEG C, and dissolved oxygen is at least 5 mg / L. According to the method, the problem that in the prior art, giant spiny frog parent reproduction and spawning cost is high, time and labor are wasted, and frog parents are damaged is solved, the problem that spawning time is interrupted, and large-batch offspring seeds with the same specification cannot be obtained at the same time is solved, the giant spiny frog inducing spawning method can guarantee giant spiny frog parent reproduction spawning size specification orderliness and enable cost to be low, and frog parents are not damaged.

The invention discloses an original ecological quasipaa spinosa culture pond. The original ecological quasipaa spinosa culture pond is composed of a pond body, the pond body is composed of a pond bottom and pond walls. The middle of the pond bottom is low and the periphery of the pond bottom is high to form a V-shaped pond bottom, the bottom of the V-shaped face of the pond bottom is provided with a water outlet, the water outlet is connected with a drainpipe disposed under the pond bottom. The periphery of the pond body is provided with feeding benches and passages, a frame is disposed over the pond body, and an inlet pipe is disposed, under the frame, in the upper portion of the bond body. The original ecological quasipaa spinosa culture pond is simple in structure, the culture pond can simulate the natural environment where quasipaa spinosa lives at the maximum, the water does not polluted by foreign materials, the quasipaa spinosa will not fall ill in the high density growth process, the survival rate is high, the quality is superior, and the original ecological quasipaa spinosa culture pond is convenient to manage.

The invention discloses a giant spiny frog culture method, and aims to solve the problems of low metamorphosis rate and low survival rate of giant spiny frogs. The method comprises the stages of tadpole incubation, tadpole metamorphosis preparation, tadpole metamorphosis, froglet culture, adult frog feeding and the like. According to the method disclosed by the invention, the giant spiny frogs are cultured at different degrees according to different growth stages, particularly nutrient enrichment is performed during tadpole metamorphosis, enough nutrition needed by metamorphosis is provided for the tadpoles, and the metamorphosis and survival rate of the tadpoles can be improved.

The invention provides a raised quasipaa spinosa hibernation treatment method, and relates to the technical field of quasipaa spinosa breeding. The method includes the following steps that (1) a quasipaa spinosa pool is set, the size of the quasipaa spinosa pool ranges from 4 m<2> to 5m<2>, the deepest position of the pool ranges from 20 mm to 50 mm in depth, the pool bottom is set to be in an inclined shape, half of the pool bottom is located above the water surface, the pool bottom face located above the water surface is provided with stones, a layer of sere phycophyta is arranged on the stones, the thickness of the phycophyta ranges from 30 mm to 70 mm, and the humidity is controlled to range from 18% to 25%; (2) feeding is conducted before hibernation, adequate feeding needs to be conducted on quasipaa spinosas before hibernation, and the proportion of the saccharides and fat in the food can be increased; (3) treatment is conducted in the hibernation process, when the temperature is lower than -5 DEG C and it is cloudy, a layer of cotton fiber is further arranged at the top of the quasipaa spinosa pool in a covering mode on the premise that the ventilation effect in the pool is guaranteed. According to the raised quasipaa spinosa hibernation treatment method, the treatment is reasonable, the hibernation time of the quasipaa spinosa can be shortened, enhancement of the immunity of the quasipaa spinosa is facilitated, and the survival rate of the quasipaa spinosa in the hibernation process is increased.

The invention belongs to the field of aquaculture, and particularly relates to a hatching method of quasipaa spinosa frogspawn. The method comprises the following steps of preparation of hatching ambient conditions, use of a hatching frame, hatching operation and cultivation of tadpole out of a membrane, wherein the hatching ambient conditions include hatching pond conditions and hatching environment, and the hatching operation comprises three-layer filtration of hatching water. The hatching ambient conditions, the use of the hatching frame, the hatching operation and the cultivation of the tadpole out of the membrane are optimized, and the illumination intensity of a hatching pond, the dissolved oxygen and pH of water in the hatching pond, the specific form of the used hatching frame, the hatching density, specific operations of the three-layer filtration of the hatching water and the cultivation of the tadpole out of the membrane are further optimized, so that the tadpole hatching density can reach 2000 tadpoles per square meter, and the tadpole hatching rate is increased, so that hatched small tadpoles are strong, and a good foundation is provided for the subsequent tadpole cultivation.

A medicine for inflammation of stone frog tadpoles and a preparation process thereof. The weight proportion of raw materials is astragalus 9%-11%, Radix Radix 2%-4%, Anemarrhena 2%-4%, Epimedium 3.5%-4.5% %, capillary 1%~3%, rhubarb 1%~4%, turmeric 0.5~1.5%, forsythia 1%~3%, Bupleurum 1%~3%, amoxicillin 17%~23%, electrolysis Multidimensional 8%~12%, enrofloxacin 16%~25%, neomycin sulfate powder 8%~12%, turmeric 8%~12%. The medicine for inflammation of stone frog tadpoles of the present invention improves the functional immunity of the gastrointestinal tract of stone frog tadpoles, promotes the proliferation of probiotics and inhibits harmful bacteria, and can regulate and restore the healthy metabolism of the gastrointestinal tract to the greatest extent during the growth of stone frog tadpoles , fundamentally solve the metabolic problem of stone frog tadpoles.

The invention belongs to the technical field of aquaculture, and particularly relates to a simulated ecological mixed culture method and system for quasipaa spinosa and acrossocheilus fasciatus. The simulated ecological mixed culture method comprises the following steps that a quasipaa spinosa culture pond is built, a plurality of ditches are built in a culture site, each ditch is provided with a water inlet and a water outlet, each water inlet and the corresponding water outlet are in communication are have a drop, and aquatic plants are planted on both sides of each ditch; cobblestones are laid in the ditches, and polished tiles and / or asbestos tiles are laid on the cobblestones; enclosing walls are built around the quasipaa spinosa culture pond with hollow bricks, wooden frames are built on the enclosing walls, and asbestine nets cover the wooden frames; an air circulation layer is arranged in the middle of the enclosing walls to realize internal and external air circulation of the simulated ecological culture room; culture water is injected into the ditches so as to keep the culture water in the ditches in a flowing state; young quasipaa spinosa and acrossocheilus fasciatus fries are stocked in the ditches; and culture baits are fed, and daily management is performed. According to the simulated ecological mixed culture method disclosed by the invention, the environment of the culture room can be consistent with the natural environment for growth of wild frogs and fishes, and transformation and upgrading of a simulated ecological culture mode are promoted.

The invention discloses a Quasipaa spinosa induced spawning and efficient breeding method and relates to the technical field of Quasipaa spinosa culture. The method comprises the steps of breeding frog selection; induced spawning, wherein the mixed solution containing chorionic gonadotropin, luteinizing hormone releasing hormone and normal saline is injected into the abdomen of each breeding frog, the use concentration of chorionic gonadotropin is 240-160 units / ml, the use concentration of luteinizing hormone releasing hormone is 7-9 micrograms / ml, 15-20 ml mixed solution is injected into each male frog, and 7-10 ml mixed solution is injected into each female frog; breeding; spawn treating, wherein a straw mat and spawns are transferred into a treating pool together, and the straw mat is beaten with a disinfectant stick to vibrate; incubation. According to the method, control over the cleanness, oxygen content and temperature of the Quasipaa spinosa breeding environment is reasonable, the fertilization rate of spawns subjected to induced spawning and spawn treating can reach over 95%, the hatchability of fertilized eggs is over 90%, effect time is shortened to 42 hours, spawn incubation time is shortened to 12.5 days, and the economic benefits of Quasipaa spinosa breeding are increased.

The invention discloses nutritional materials of a quasipaa spinosa tadpole feed and relates to the technical field of breeding of quasipaa spinosa. The nutritional materials are characterized by comprising the following components in parts by weight: 50-60 parts of pure milk, 8-10 parts of alga plant powder, 20-30 parts of fish meal, 10-15 parts of silkworm chrysalis meal, 1-2 parts of flavomycin, 8-10 parts of glucose, 3-6 parts of calcium powder, 1-2 parts of vitamin C and 1-2 parts of carotene. The nutritional materials disclosed by the invention are reasonable and comprehensive in nutrition, has certain capability of improving water quality and oxygenating water, and is capable of guaranteeing a high survival rate of tadpoles and thus increasing the economic benefits.

The invention belongs to the technical field of ranid culture, and discloses a quasipaa spinosa culture method. The quasipaa spinosa culture method comprises the following steps: culture land construction: arranging a plurality of feeding devices in a rearing pool, and by using the characteristic that baits are driven by the feeding devices to move along a vertical direction so as to be convenient to perceive by froglets, thereby improving feeding effects and shortening the growing period of the froglets; a frog spawn incubation stage: setting a condition suitable for tadpoles to incubate, and cleaning lesion frog spawns so as to prevent healthy frog spawns from being infected; a tadpole transformation stage; an adult raising stage. According to the quasipaa spinosa culture method, the problem that quasipaa spinosa slowly grows since quasipaa spinosa feeding effects are poor in the prior art is solved.

The invention discloses quasipaa spinosa incubating method and equipment.The method includes the following steps: preparing before incubation: using an aerator to aerate a water body, and controllingthe water temperature at 20-22 DEG C; placing spawning tilestones: placing the tilestones in a spawning box, wherein each tilestone floats on the surface of the water body; taking the tilestone for incubation: after the tilestone is filled with quasipaa spinosa eggs in 1-2 days, moving the entire spawning tilestone to an incubator; incubating: controlling the water body temperature of the incubator at 22-28 DEG C, and finishing the incubation of the batch of quasipaa spinosa eggs in 10-15 days; breeding: placing incubated young tadpoles in a breeding room for breeding; treating after incubation:placing the tilestones incubating the young tadpoles in a cleaning box for cleaning, and then airing the tilestones for standby use.The invention provides the continuous and stable incubating method; the problems of low quasipaa spinosa incubating speed, high artificial strength, and low incubating rate are solved.

The invention discloses a hibernation treating method for raising quasipaa spinosa and relates to the technical field of quasipaa spinosa culture. The hibernation treating method comprises the following steps of: (1) arranging a frog pond, wherein the size of the frog pond is 4-5m<2>, the deepest position of water is 20-50mm, the bottom of the pond is arranged into an inclined shape, a half of the bottom of the pond is positioned above the water level, stone blocks are arranged on the bottom surface of the pond above the water level, a layer of withered algae plant is arranged on the stone blocks, the thickness of the algae plant is 30-70mm and the humidity is controlled to be 18-25%; (2) feeding before hibernation, wherein full food are fed to the quasipaa spinosa before hibernation, and the proportion of sugar and fat in food can be increased; (3) treating in hibernation, wherein when the weather temperature is lower than minus 5 DEG C and the weather is cloudy, a layer of cotton wool is enabled to cover the top part of the frog pond, but the breathable effect in the pond needs to be ensured. The hibernation treating method disclosed by the invention has the advantages that the treatment is reasonable, the hibernation time of the quasipaa spinosa can be shortened, and the benefit for improving the resistance of the quasipaa spinosa and increasing the survival rate of the quasipaa spinosa in the hibernation is achieved.

The invention relates to the technical field of quasipaa spinosa cultivation, and provides a bacteriostatic traditional Chinese medicine cultivation method for adult quasipaa spinosa. The cultivation method is characterized by including the following steps of building an adult quasipaa spinosa pool, wherein the size of the pool is 4-5 m<2>, the largest depth of water is 0.5-1 m, the bottom of the pool is arranged in an inclined mode, the inclination angle of the bottom of the pool is smaller than 30 degrees, and fresh leaves of Chinese ilex are put at the combining position of the bottom of the pool and the water surface in the pool and are pressed flat; throwing and feeding the adult quasipaa spinosa, wherein starting from the moment that tadpoles transform into froglets, the body weight can reach 250-300 g after the froglets are fed for 5-6 months and can reach 450-500 g to the maximum after the froglets are fed for 8-10 months; hibernating in winter, wherein the hibernation of the quasipaa spinosa ranges from November to February of the next year, thick fallen leaves are additionally arranged at the bottom of the pool to facilitate hibernation, stones are placed on a slope at the bottom of the pool, gas caves are formed between the stones, and a heat preservation cover plate is arranged above the stones. The bacteriostatic traditional Chinese medicine cultivation method does not need frequent disinfection, management is convenient, the imitating wild environment is good, growth of the quasipaa spinosa is facilitated, and the economic benefits of quasipaa spinosa breeding can be increased easily.

The invention relates to a frog ecological breeding apparatus for quasipaa spinosa and a breeding method for quasipaa spinosa. The frog ecological breeding apparatus for quasipaa spinosa comprises a preparative frog pool, a frog pool and a voice playing device, wherein a shading net mask is arranged on the frog pool, sound-insulation plates are installed at the outer sides of the frog pool, and the voice playing device is installed at the bottom of the frog pool. According to the invention, aiming at different growth stages of the quasipaa spinosa, pools are divided into two types, namely, the preparative frog pool and the frog pool. In order to control the mating time and improving the mating rate, the outer sides of the frog pool are provided with the sound-insulation plates, and quasipaa spinosa mating is induced through sounds. By enriching nutrients, arranging cobblestones, stone caves and other hidden things in water, and planting acorus calamus and other aquatic plants in water for optimizing the environment in breeding pools, the survival rate of frogs and the quality of frog eggs are improved.

The invention discloses a quick and zero-damage quasipaa spinosa sampling method. According to the method, by means of the oral epidermal cells of quasipaa spinosa and the characteristic of mucus secretion and by adopting homemade sterilization plastic film swabs, intraoral cells of quasipaa spinosa are sampled, oral cells which are taken out are placed in a centrifugal pipe, PBS is added to enable oral cells to be fully blended in PBS, and then sample DNA is extracted by means of a genomic DNA Mini Kit. The method has the advantages that damage to the quasipaa spinosa body is small, the sampling speed is high, the death rate is low, the DNA extraction effect is good, and the method is simple and easy to operate.

The invention discloses a green breeding method of quasipaa spinosa. The method comprises five steps including setting of a breeding farm environment, setting of breeding facilities, cultivation of frog bodies, tail water treatment and disease control. Breeding waste water generated in all breeding ponds is discharged into a tail water treatment pond, the water depth is controlled to be 60-80 centimeters, and epinephelus fingerlings are stocked in the tail water treatment pond, wherein the body length of each fingerling is 5-8 centimeters, and the stocking density is controlled to be 50-70 fingerlings per square meter; aquatic plants are planted in the tail water treatment pond, and disease treatment involves treatment on saprolegniasis, enterogastritis and skin ulceration diseases. By means of the green breeding method of the quasipaa spinosa, the survival rate of the quasipaa spinosa including tadpoles, froglings and adult frogs can be greatly increased, the discharge of high-concentration breeding waste water is effectively avoided, and the economic benefit can also be increased.