The invention discloses an artificial pairing and breeding method of platysternon megacephalum. The method comprises the steps of: provenance selection, domestication before pairing, setting of a breeding pool, pairing and breeding, collection and incubation of eggs and the like. The method takes full account of the ecological and biological characteristics of platysternon megacephalum, including ethological characteristics, diet characteristics, habitat characteristics, breeding ecological characteristics and the like. The method can effectively solve the major problems in the current artificial pairing and breeding of platysternon megacephalum, including the problems in seed selection, pairing, selection of pairing time, sex ratio, density, bait, setting of breeding and incubation environment and the like; the occurrence of impairment and death in the breeding mating process is obviously reduced, the egg laying rate of female platysternon megacephalum and the incubation rate of eggs are obviously increased, artificial efficient breeding of platysternon megacephalum is realized, healthy platysternon megacephalum seedlings are obtained, and a technical support is provided for expanding the breeding population of platysternon megacephalum, promoting species protection and developing large-scale breeding; and the method requires little human input, has low cost, is easy to operate, and is favorable for popularizing the artificial breeding of platysternon megacephalum.