91 results about "Hermaphrodite" patented technology

The invention relates to a method for cultivating a new variety of hybridized and backcrossed Argopecten purpuratus. The method is characterized by comprising the following steps of: selecting excellent individuals in a hybridized first filial generation family or group of Argopecten irradians and the Argopecten purpuratus; backcrossing the excellent individuals with the Argopecten purpuratus to establish a backcrossed family or group; selecting hermaphroditic excellent individuals with good representation in the backcrossed family or group; and continuously cultivating at least third generations of self-bred families to obtain the new variety of the hybridized and backcrossed Argopecten purpuratus having the advantages of steady heredity, rapid growth, large individuals and wide temperature tolerance range. According to the method provided by the invention, the new variety can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 30%.

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossed scallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparison with the current yield of Argopecten irradians.

A seismic cable coupling comprising connector body, a coupling ring is rotatably mounted on the connector body. The coupling ring includes a ring body having a first longitudinal projection and a second longitudinal projection, the first longitudinal projection having an interior surface including a first angled groove, the second longitudinal projection having an exterior surface including a first raised stud. The coupling ring is a hermaphrodite coupling ring that mates with a coupling ring having a substantially identical ring body.

The invention discloses technology for culturing a unisexual hybrid scallop by utilizing a purple scallop and a bay scallop, which relates to shellfish breeding technology for aquiculture. The technology comprises the steps of: utilizing two congeneric hermaphroditic scallops with same chromosome number; ripening the two scallops respectively to achieve the synchronous maturity; and then synchronously inducing the two scallops to spawn and spermiate; and acquiring sperms and ovum of the two scallops respectively for interspecies cross-insemination to culture the unisexual hybrid scallop. Obtained offsprings have separated sexes, have males and females, and also have hermaphroditic individuals; ovum from female individuals in the hybrid offsprings can be inseminated by any parental sperm and normally grow; and the hermaphroditic individuals in the hybrid offsprings can be used for quickly purifying a strain.

The invention relates to a papaya full exposure fast cutting propagation method, characterized in that, hermaphrodite carica papaya tree is used as female parent, an upper part of the tree is cut from the lowest part, then the female parent is stubbed and transported to a field gene bank, a root part is processed by radication agent containing 6-benzyl adenine 0.05-0.2 mg, indolebutyric acid 0.5-1.0 mg per liter; a tree body is sprayed with polysulfide suspending agent and bud advancing agent containing 6-benzyl adenine 5.0-10.0 mg and triiodobenzoic acid 0.5-1.0 mg, causing a parent tree to come out lateral buds, mowing down the lateral buds as root taking agent soaked for cutting shoot; a base part is bedaubed with rootone containing naphthylacetic acid 50-100 mg and indolebutyric acid 100-200 mg per kilogram, inserted to a planting screen, transplanting to the nutrient cup after the shoot to be inserted comes out adventitious roots and new leaves, then permanently planted in the field. The invention is small in investment, short in time, tree percent is more than 80%, root taking rate and survival rate reach more than 85%, thereby providing effective approach for meeting with the requirement of the germchit market.

The invention aims to provide a method for sex induction of hermaphrodite shrimps. According to the method, a target gene in macrobrachium is silenced by using an RNA interference technology to realize sex induction of macrobrachium rosenbergii, wherein the nucleotide sequence of the target gene is SEQ ID NO:1. By virtue of the induction technology provided by the invention, a macrobrachium individual has sex reversion and becomes hermaphrodite macrobrachium rosenbergii, and the developments of a female reproductive system and a male reproductive system are realized in the body of the hermaphrodite macrobrachium rosenbergii, so that a set of effective macrobrachium rosenbergii sex induction technology is established. Meanwhile, by using the hermaphrodite shrimps cultured by the method provided by the invention, a good scientific research material and an animal sample can be provided for sex determination, sex differentiation and researches on reproduction and fertilization of shrimps and crabs.

A seismic cable coupling comprising connector body, a coupling ring is rotatably mounted on the connector body. The coupling ring includes a ring body having a first longitudinal projection and a second longitudinal projection, the first longitudinal projection having an interior surface including a first angled groove, the second longitudinal projection having an exterior surface including a first raised stud. The coupling ring is a hermaphrodite coupling ring that mates with a coupling ring having a substantially identical ring body.

The invention relates to a construction method and application of the maintainer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The maintainer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus to have the characteristics of male sterility and ovum fertility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that firstly, a large number of the first filial generations of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are constructed, and male sterile individuals are found out from the first filial generations for breeding of the male sterility line; then sperms of male parent Argopecten irradians concentricus are used for continuous backcross, and the combinations of the male sterility line-maintainer line with all generations of male sterility individuals are respectively bred; finally, the male sterility line parent scallops are produced through hybridization of the male sterility line and the maintainer line, and the maintainer line is used for producing commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the maintainer line of the scallops is firstly built in animals, and the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated.

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossedscallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparisonwith the current yield of Argopecten irradians.

The invention provides a method for setting up dioecism type mussel inbred line which consists of selecting hermaphroditic special individuals from gonochorite seashells, establishing and cultivating selfing lines through the strategy of self crossing, the invention can be applied for accelerating the breed choosing and cultivating speed of gonochorite shells.

The invention discloses an efficient female method for industrial hemp plants. The efficient female method comprises the steps of treating industrial hemp female plants by using nano-silver colloid spray, inducing the industrial hemp female plants to be converted into hermaphrodite plants, differentiating male flower buds, generating fertile pollen, and then freely pollinating and setting seeds to obtain all-female industrial hemp seeds. According to the efficient female method, nano-silver colloid is adopted to induce the plants, the female hemp plants are converted into the hermaphrodite hemp plants, the female plants are forced to generate the fertile pollen to pollinate other female plants, and the pure female (or all-female) industrial hemp seeds with the purity of 96% or above can be obtained. The amount of required colloidal silver solution is small, the induction cost is reduced, the procedure is simplified, and the induced feminization success rate is high and stable.

A payment card comprises a supporting body with a controller provided with an inner memory in which identification data of a user and the issuing institution are stored. In addition the card provides a connection interface combined with the controller for the connection with an outside device. The connection interface is a connection port incorporating a male portion and a female portion arranged, one with respect to the other, according to a specific layout rendering such a port a “hermaphrodite” port, that is able to couple with a port having an identical geometry. The connection port further comprises a plurality of electric contact elements combined with the male portion and / or the female portion. A method referring to virtual transfer of money between two payment cards is further described.

The invention relates to cultivation and application of huabei all-female No. 2 variety of an all-female huabei type cucumber, which belong to the field of biotechnology breeding. The cultivation comprises the steps of: crossing all-female European greenhouse type cucumber EC1 with hermaphrodite huabei type cucumber P02, backcrossing the obtained F1 with the PO2, selecting all-female single plants from the backcross generation and performing continuous backcross with the hermaphrodite huabei type cucumber P02 to generate four generations; selecting the all-female single plants from BC4F1 to perform selfing; selecting the all-female single plants from BC4F2 to perform continuous selfing; and finally, selecting the all-female plants from BC4F3 and allowing the cucumbers of the plants to perform mixed pollination to obtain a new all-female huabei type cucumber variety, namely huabei all-female No.2 variety which grows quickly and has excellent quality and high yield. An all-female cucumber variety with high seed purity and relatively strong hybrid vigor can be obtained by crossing the cucumbers of the huabei all-female No.2 variety and the commonly cultivated cucumbers.

This technology relates to measuring tools, and provides a linear size measuring tool, especially a direct reading integrated inner and outer calipers, which have two states of inner clamp and outer clamp, including a pair of clamp arms that are symmetrically arranged and cross-hinged by rotating pins. One side of each pincer arm is a clamping section, and the other side is a reading section; at the end of the clamping section, there is a chuck that can adjust the angle between the clamp arm and the clamp arm in a plane determined by a pair of clamp arms. The two chucks with pointed fronts are arranged symmetrically. When the chuck is inclined inward, it is an inner card; ;When the chuck tilts inward to the limit position, the included angle between the chuck axis and the clamping segment axis is 12°; when the chuck tilts outward to the limit position, the included angle between the chuck axis and the clamping segment axis is 25°.

The invention relates to a construction method and application of an argopecten purpuratus and argopecten irradians irradians hybridized scallop restoring line. The restoring line belongs to an argopecten irradians irradians strain which enables male sterile line progenies to restore the male fertile property, wherein argopecten purpuratus and argopecten irradians irradians hybridized scallop belongs to hybrid progenies of argopecten purpuratus ovums and argopecten irradians irradians sperms. The construction method comprises the steps that excellent individuals in the male sterile line are selected, which are continuously backcrossed through different argopecten irradians irradians until all progenies are hermaphrodite, then the restoring line of the male sterile line is adopted, and finally the restoring line and corresponding male sterile line are subjected to hybridization, so that argopecten purpuratus and argopecten irradians irradians hybridized scallop product seedlings are produced. The method establishes a male sterile line restoring line in animals for the first time, and effectively overcomes the obstacle of autofertilization during hermaphrodite scallop hybridization, and the annual yield of the produced argopecten purpuratus and argopecten irradians irradians hybridized scallops is increased by 40 percent above compared with that of argopecten irradians irradians.

The invention discloses a hermaphrodite laver dihaploid colony building method. The method comprises taking hermaphrodite laver thalluses (gametophytes) with obvious color difference as cross parents, carrying out mixed cultivation on father and mother thalluses with antheridiums, collecting carpospores, selecting the carpospores with germ tubes, carrying out single culture to obtain protonema (sporophore), carrying out induction to obtain conchospore of the protonema, carrying out culture to obtain macroscopic F1 thalluses, wherein if 90% or more of the thalluses contain amphiphilic color chimeras, the thalluses are used for building of a dihaploid colony, separating all color blocks of the four-color block chimera, acquiring monospores or single isolated cells of each one of the color blocks, carrying out culture to obtain macroscopic thalluses, carrying out single thallus culture and autofertilization to obtain pure thallus of each one of color blocks of the four-color block chimera and building a dihaploid colony. The method solves problems of hermaphrodite laver dihaploid colony building and lays the foundation of genetic linkage map construction.

The invention discloses a breeding method for scallop hybridization breeding. The breeding method is characterized by comprising the steps that firstly, gonad development synchronous breeding is carried out on selected chlamys farreri and Argopecten irradians; then, fully-developed female chlamys farreri in the chlamys farreri and the fully-developed Argopecten irradians are selected, after the female chlamys farreri finishes spawning, the hermaphroditic Argopectehs irradians is set for spermiation, and fertilization is carried out on sperms and eggs to form scallop hybridization fertilized eggs; incubation is carried out on the hybridization fertilized eggs, then larva selection is carried out, and larvas are bred in a larva breeding pond; finally the bred larvas are bred to scallop hybridization fry capable of being bred through settlement and metamorphosis. The hybridization scallop fry are bred from the female chlamys farreri and the Argopectehs irradias in a hybridization mode, and have the prominent advantages that in the breeding process, growing speed is high, resistance to diseases is high, the gonad is poor in fertility, the adductor muscle is large, the growing speed can be improved by more than 30 percent, the yield of primary products of the adductor muscle is improved by more than 40 percent, the obvious heterosis is achieved, and the sustainable development of scallop culture in China can be promoted.

The invention relates to a construction method and application of the restorer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The restorer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line to restore the characteristic of male sterility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that excellent individuals in the male sterility line are selected, different varieties of Argopecten irradians concentricus are used for continuous backcross until all generations are hermaphrodite, and the restorer line of the male sterility line is obtained; finally, the restorer line and the corresponding male sterility line are used for hybridization to produce commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the restorer line of the male sterility liner is firstly built in animals, the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated, and the yield of the produced hybrid scallops of the purple scallops and the Argopecten irradians concentricus is improved by more than 42 percent relative to that of the Argopecten irradians concentricus in the same year.

The present invention provides an all hermaphroditic papaya variety and their seeds, pollans, tissue cultures, parts of plant and the method for producing them.

The current invent relates to the construction and application of a three-line breeding system in the bay scallop×Peruvian scallop hybrids. The said three-line consists of a male sterile line, a maintainer line and a restorer line. A combination of male sterile line and maintainer line is obtained by continuously backcrossing the male sterile individuals selected from F1 inter-specific hybrid families with sperm of the selfing family of the sperm-providing Peruvian scallops until the progenies are all male sterile. The restorer line is obtained by continuously backcrossing selected individuals from the male sterile line with sperm of the selfing family of a Peruvian scallop until the progenies are all hermaphroditic and exhibit excellent production traits. Commercial male sterile brood stocks are produced by backcrossing the male sterile line with the maintainer line and commercial hybrid spats are produced by backcrossing the male sterile line and the restorer line.

The present invention provides an all hermaphroditic papaya variety and their seeds, pollans, tissue cultures, parts of plant and the method for producing them.

The invention relates to an establishing method of a Cnobilis inbred line, which can quickly purify the genome of the Cnobilis, so as to accelerate variety breeding. Individuals containing female and male germ cells and appearing as female, namely hermaphrodites, are selected through micrographic examination, an egg is fertilized by sperm in the same individual through induced parturition hastening or dissection fertilization, the amount of the sperms are increased or reduced artificially by taking measures to artificially interfere and adjust the relative proportion of the male and the female gametes in the water, and hatching, breeding and cultivating are carried out according to the ordinary technology of the species, so as to establish the inbred line. The establishing method of the Cnobilis inbred line is simple to operate, requires no special chemical reagent, and is easy to popularize and apply.

The invention relates to an establishment method and an application of a violet black hybrid scallop restorer. The restorer is a violet scallop system which enables the male sterile line offspring to restore the male fertile feature, wherein the violet black hybrid scallop is a hybrid offspring of Argopecten irradians concentricus eggs and violet scallop sperms. The establishment method comprises the steps of selecting excellent individuals in the male sterile line, continuously backcrossing different violet scallop sperms, and obtaining the restorer of the male sterile system until all offspring are hermaphrodite; and finally hybridizing the restorer with corresponding male sterile line to produce the violet black hybrid scallop commodity offspring seed. The male sterile restorer is firstly established in animals, the obstacle of autofertilization in the hybridization of the hermaphrodite scallops can be effectively overcome; the annual yield of the produced violet black hybrid scallop is improved by more than 45 percent than that of the Argopecten irradians concentricus.

Disclosed is a method for production of hybrid seeds of laver filament pure lines characterized in that the method comprises the steps of screening individuals with dissimilar sex character from feral population, scrubbing with absorbent cotton balls, filtering excess seawater, cutting down 1-2mm squared algae slices on the base portion of the hermaphroditic algae, confirming that all the cells are nurse cells, supersonic cleaning, culturing under the condition of 10-14 hours light lamination, and separating single algae for expansion.

Papaya plants containing mutant allele EWSMHP, which confers production of highly hermaphroditic progenies upon selfing of its hermaphrodite plants and also production of highly hermaphroditic F1 progenies when crossed with normal female and normal hermaphrodite papaya plants, are disclosed. The invention relates to the seeds of papaya plants having mutant allele EWSMHP, to the plants and plant parts of papaya plants having mutant allele EWSMHP and to methods for producing progeny of papaya plants having mutant allele EWSMHP. The invention also relates to methods for producing a papaya plant having mutant allele EWSMHP containing in its genetic material one or more transgenes and to the transgenic papaya plants and plant parts produced by those methods. The invention also relates to papaya cultivars or breeding cultivars, and plant parts derived from papaya plants having mutant allele EWSMHP. The invention further relates to hybrid papaya seeds, plants, and plant parts produced by crossing a plant having mutant allele EWSMHP with another papaya cultivar.

A sex identification method for veneridae shellfish parents comprises the following steps: preparing a syringe with a syringe needle, preparing a to-be indentified veneridae shellfish, finding the intersection point of the lateral ligament end and the shell edge and taking the point as an acquisition point, stably handling the to-be indentified veneridae shellfish by using one hand, and using the other hand to align the syringe needle of the syringe with the acquisition point, and enabling the syringe needle to have an angle of 30-60 degree with the tangent of the shell edge, penetrating the syringe needle into the sexual gland position of the to-be indentified veneridae shellfish through the acquisition position, extracting a sample, putting the sample on slide glass with seawater, microscopically observing the form of the sample, if the sample is divergent in seawater and has circular or pear-shaped ova, identifying the indentified veneridae shellfish is female, if the sample is in a smog state in seawater and has polliwog-shaped sperms, identifying the indentified veneridae shellfish is male, and if the sample comprises circular or pear-shaped ova and polliwog-shaped sperms, identifying the indentified veneridae shellfish is hermaphroditic.

A method for breeding high-purity laver line includes decomposing the immature laver leaf by mussel protein extract liquor to generate its unicells or protoplasts, culturing or inducing chromosomal multiplication to obtain pure line of diploid filamnets, culturing until sexual mature, self fertilization to generate homozygous diploid filaments, and culturing the certified seeds or seedlings. Its advantages are excellent individual characteristics, and high yield and quality of laver.

Disclosed are a hybrid populous lasiocarpa and a planting method thereof. The hybrid populous lasiocarpa is obtained by hybridizing and breeding Chinese white poplar and European red poplar and named as environmental-protection Chinese populous lasiocarpa. The planting method comprises the steps of seedling, cottage, transplanting in forest establishment regions, maintenance and forest establishment. The e environmental-protection Chinese populous lasiocarpa has a timber cycle of 3-6 years and has the advantages of being hermaphrodite, flowerless, seedless, saline-alkaline-resistant, wind-resistant, disease-resistant, pest-resistant, wide in application range, rapid in growth, harmless to air and soil, high in timber yield and the like, thereby being capable of serving as environmental greening, city and road border tree or windbreak as well as economic tree for large-area mountains or hills. The good environmental effects, the high timber yield, the good material quality and the comprehensively-applicable economic value can generate high social and economic benefits.