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Establishment method and application of violet black hybrid scallop restorer

A method for constructing a restorer line, which is applied in the field of shellfish breeding technology--hybrid scallop commercial seedling cultivation, can solve the problems of inability to establish male sterile lines, maintainer lines and restorer lines, and achieves overcoming obstacles to self-fertilization, low cost effect

Active Publication Date: 2014-12-17
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so far, so it is impossible to establish male sterile lines, maintainer lines and restorer lines similar to crops for hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Breeding of the Mozi hybrid scallop restorer line: Select 50 large individual scallops from the male sterile line of the Mozi hybrid scallop, fertilize them with sperm from different purple scallops after promoting maturation and oviposition, and establish at least 20 backcross families At the same time, the purple scallops for sperm were self-propagated to establish an inbred family, and the growth and survival of the backcross offspring were measured, and the combining ability test was carried out; from the second year onwards, according to the results of the combining ability test and the development of the gonads, one of them with excellent traits and good traits was selected every year. For the backcross family containing hermaphroditic scallops, the seed shells of the related male sterile family will be continuously backcrossed with the sperm of the purple scallop hybrid family for sperm to establish a backcross family until all the backcross families are hermaph...

Embodiment 2

[0025]In 2011, using the imported purple scallop and the Gulf of Mexico scallop from Zhanjiang, Guangdong, a first-generation orthogonal and a reciprocal family of interspecific hybrids were co-bred. According to actual measurements, the average shell height of the first generation of Mozi hybrid scallops (Gulf of Mexico scallop eggs fertilized with purple scallop sperm) is 52.22 mm, body weight is 25.49 grams, and column weight is 2.19 grams, while the average shell height of Gulf of Mexico scallops cultured in the same period is 42.02 mm, with a body weight of 14.80 grams and a column weight of 1.13 grams. Compared with the Gulf of Mexico scallops of the same period, the first generation of Mozi hybrids had a shell height of 24.3%, a body weight of about 72.2%, and a column weight of 93.8%. In the spring of 2012, after promoting the maturation of the first-generation hybrid scallops, select scallops that are large in size and only develop in the female area. After drying in t...

Embodiment 3

[0029] In 2014, a total of 16 first-generation hybrid families of Gulf scallop eggs and purple scallop sperm were established using Gulf scallops and purple scallops, and self-bred families of purple scallops for sperm were established. According to the method in Example 1, you can The male sterile line of Mozi hybrid scallop and its corresponding maintainer and restorer lines are selected within 1-3 years to realize the commercial production of Mozi hybrid scallop seedlings.

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Abstract

The invention relates to an establishment method and an application of a violet black hybrid scallop restorer. The restorer is a violet scallop system which enables the male sterile line offspring to restore the male fertile feature, wherein the violet black hybrid scallop is a hybrid offspring of Argopecten irradians concentricus eggs and violet scallop sperms. The establishment method comprises the steps of selecting excellent individuals in the male sterile line, continuously backcrossing different violet scallop sperms, and obtaining the restorer of the male sterile system until all offspring are hermaphrodite; and finally hybridizing the restorer with corresponding male sterile line to produce the violet black hybrid scallop commodity offspring seed. The male sterile restorer is firstly established in animals, the obstacle of autofertilization in the hybridization of the hermaphrodite scallops can be effectively overcome; the annual yield of the produced violet black hybrid scallop is improved by more than 45 percent than that of the Argopecten irradians concentricus.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to a construction method and application of a restorer line of Mozi hybrid scallop (the hybrid offspring of Gulf of Mexico scallop ova and purple scallop sperm). Background technique [0002] The Gulf of Mexico scallop (Argopecten irradians concentricus) is native to the coast of the Gulf of Mexico in the United States and was introduced to my country in the 1990s. The Gulf of Mexico scallop grows fast and adapts to a wide range of temperatures. It is especially suitable for high-temperature sea areas and has strong stress resistance. It is very suitable for farming in the southern seas of my country. It is currently the main scallop cultured in the southern seas. However, due to the long-term inbreeding after the introduction, the germplasm of the Gulf of Mexico scallop has been seriously degraded during the br...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王春德马斌孙奎府赵玉明刘博周顺王文琪赵侠苗国英
Owner QINGDAO AGRI UNIV
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