73 results about "Argopecten irradians" patented technology

The invention relates to a method for cultivating a new variety of hybridized and backcrossed Argopecten purpuratus. The method is characterized by comprising the following steps of: selecting excellent individuals in a hybridized first filial generation family or group of Argopecten irradians and the Argopecten purpuratus; backcrossing the excellent individuals with the Argopecten purpuratus to establish a backcrossed family or group; selecting hermaphroditic excellent individuals with good representation in the backcrossed family or group; and continuously cultivating at least third generations of self-bred families to obtain the new variety of the hybridized and backcrossed Argopecten purpuratus having the advantages of steady heredity, rapid growth, large individuals and wide temperature tolerance range. According to the method provided by the invention, the new variety can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 30%.

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossed scallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparison with the current yield of Argopecten irradians.

The invention relates to a method for cultivating hybridized and backcrossed marine product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten purpuratus and Argopecten irradians, and synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten irradians; inducing the hybridized first filial generation individuals to spawn by using a conventional shade drying and temperature stimulation method so as to obtain unfertilized eggs; simultaneously, stimulating the Argopecten irradians to spawn and discharge seminal fluid, and filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten irradians; and fertilizing the hybridized first filial generation eggs with the seminal fluid of the Argopecten irradians, cultivating according to a conventional method after hatching germ cells, and thus obtaining the hybridized and backcrossed marine product fries which are above 1.5 cm in shell height and used for commercial cultivation. The hybridized and backcrossed marine product fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed marine product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed marine product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention relates to a method for cultivating hybridized and backcrossed Argopecten product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten irradians and Argopecten purpuratus; synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten purpuratus; inducing the hybridized first filial generation individuals to ovulate by using a conventional shade drying and temperature stimulation method; simultaneously, stimulating the Argopecten purpuratus to spawn and discharge seminal fluid; filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten purpuratus; fertilizing the seminal fluid of the Argopecten purpuratus with the hybridized first filial generation eggs; carrying out larva cultivation and fry intermediate cultivation according to a conventional method after hatching; and thus obtaining the hybridized and backcrossed Argopecten product fries having obvious heterosis. The hybridized and backcrossed marine Argopecten fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed Argopecten product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed Argopecten product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

Provided is a Korea and Mexico gulf scallop hybrid cultivation method. At the first year, good parent scallops are selected, maturation acceleration is performed on the parent scallops, ova of single Korea gulf scallop and sperms of single Mexico gulf scallop are collected and subjected to mixed insemination to establish Korea and Mexico gulf scallop first filial generation families, and a conventional gulf scallop breeding, keeping and offshore cultivation method is adopted to perform breeding; at the second year, good hybridization families are selected according to the phenotypes of the hybridization families obtained at the first year, good-phenotypic-trait individuals are selected from the good hybridization families, self-fertilization is performed for purification after maturation acceleration, and accordingly multiple Korea and Mexico gulf scallop first filial generation inbred families are established; the same method is adopted to continuously perform inbreeding for more than five generations, namely new variety of stable-inheritance, quickly-growing and large-sized scallops wide in temperature resisting range is obtained. The new variety simultaneously has the good advantages of the Korea gulf scallops and the Mexico gulf scallops. In addition, Korea and Mexico gulf scallop hybrids can be bred in most of sea areas in our country, the yield is improved by higher than 30% compared with the yield of Mexico gulf scallops bred in the southern of our country, and the Korea and Mexico gulf scallop hybrid cultivation method has great economic value.

The invention relates to a selective breeding method for a hybridized-Argopecten irradians backcross low-temperature-resistant scallop line. The method is characterized by comprising the steps: firstly, selecting individuals, which are high in growth speed and high in low-temperature resistance, from a family or population of a first filial generation of Argopecten irradians and Argopecten purpuratus as parent scallops; carrying out back crossing on individuals, with high low-temperature resistance, of the first filial generation and Argopecten irradians ova so as to establish a backcross family or population; and then, carrying out selective breeding on low-temperature resistant breeding core groups for at least three times. According to the method, the first filial generation which is high in growth speed and remarkable in heterosis is adopted as a male parent and then is subjected to back crossing with the Argopecten irradians (a female parent) so as to polymerize a chilling-resistant gene of the Argopecten irradians to a first generation of hybridized-Argopecten irradians backcross, the individuals, with high low-temperature resistance, of the first generation of hybridized-Argopecten irradians backcross are taken as a breeding core group and is subjected to continuous 3 generations of low-temperature resistant breeding, the low-temperature resistance is extended to 0 DEG C, the growth speed is increased by 15% compared with that of the Argopecten irradians, and then, a low-temperature resistant new line which is applicable to wintering of Shandong sea area is obtained. Apparently, the method is simple and convenient in operation and is extensively applied to the selective breeding of hybridized low-temperature resistant scallop lines.

A method for extracting polysaccharide from Argopecten irradians comprises processing raw material, extracting with water, separating, concentrating, precipitating with ethanol and drying, which belongs to the technical field of food science. Specifically, the method comprises pounding Argopecten irradians tissue, oven drying or freeze drying, soaking in a reagent, and refluxing for removing oligosaccharide and pigment; extracting with water as a solvent; centrifuging to obtain a supernatant; performing vacuum rotary evaporation and concentration; adding anhydrous ethanol into the obtained concentrated solution for precipitating polysaccharide when the ethanol reaches a certain of concentration; and drying. The inventive method has high yield. The obtained product has high purity, is easy for further separation and purification, and can be directly used as a product or be processed into tablets and capsules. The invention is of far reaching importance to high-value processing of Argopecten irradians and development of functional food.

The invention relates to a construction method and application of the maintainer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The maintainer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus to have the characteristics of male sterility and ovum fertility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that firstly, a large number of the first filial generations of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are constructed, and male sterile individuals are found out from the first filial generations for breeding of the male sterility line; then sperms of male parent Argopecten irradians concentricus are used for continuous backcross, and the combinations of the male sterility line-maintainer line with all generations of male sterility individuals are respectively bred; finally, the male sterility line parent scallops are produced through hybridization of the male sterility line and the maintainer line, and the maintainer line is used for producing commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the maintainer line of the scallops is firstly built in animals, and the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated.

The invention relates to a culture method of Mexico and Korea hybrid scallops. According to the method, in the first year, good seed scallops are selected and are subjected to ripening acceleration, then, ova of a single Mexico gulf scallop and sperms of a single Korea gulf scallop are respectively collected, and are mixed for insemination, a Mexico and Korea hybrid first generation family is built, and conventional gulf scallop fry culture, fry preservation and sea cultivation methods are used for culture; in the second year, good hybrid families are selected according to the expression types of each hybrid family obtained in the first year, individuals with good properties are selected from the good hybrid families, and after the ripening acceleration, the autofertilization is respectively carried out for purification, so a selfing family of a plurality of Mexico and Korea hybrid sub first generations is built; selfing is continuously carried out for more than five generations by the same method, and a new scallop variety with the advantages of stable heredity, high growth speed, great individual size and wide temperature-resistant range can be obtained. The scallops in the variety simultaneously have the excellent characteristics of the Mexico gulf scallops and the Korea gulf scallops, and can be cultured in most sea area in China, the yield is improved by more than 40 percent through being compared with that of the Mexico gulf scallops cultured in south China, and great economic values are realized.

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossedscallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparisonwith the current yield of Argopecten irradians.

The invention relates to a method for cultivating hybridized and backcrossed marine product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten purpuratus and Argopecten irradians, and synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten irradians; inducing the hybridized first filial generation individuals to spawn by using a conventional shade drying and temperature stimulation method so as to obtain unfertilized eggs; simultaneously, stimulating the Argopecten irradians to spawn and discharge seminal fluid, and filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten irradians; and fertilizing the hybridized first filial generation eggs with the seminal fluid of the Argopecten irradians, cultivating according to a conventional method after hatching germcells, and thus obtaining the hybridized and backcrossed marine product fries which are above 1.5 cm in shell height and used for commercial cultivation. The hybridized and backcrossed marine productfries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed marine product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed marine product fries canbe cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention relates to a method for cultivating hybridized and backcrossed Argopecten product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten irradians and Argopecten purpuratus; synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten purpuratus; inducing the hybridized first filial generation individuals to ovulate by using a conventional shade drying and temperature stimulation method; simultaneously, stimulating the Argopecten purpuratus to spawn and discharge seminal fluid; filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten purpuratus; fertilizingthe seminal fluid of the Argopecten purpuratus with the hybridized first filial generation eggs; carrying out larva cultivation and fry intermediate cultivation according to a conventional method after hatching; and thus obtaining the hybridized and backcrossed Argopecten product fries having obvious heterosis. The hybridized and backcrossed marine Argopecten fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed Argopecten product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed Argopecten product fries can be cultivated in sea areas inthe north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention relates to a construction method and application of an argopecten purpuratus and argopecten irradians irradians hybridized scallop restoring line. The restoring line belongs to an argopecten irradians irradians strain which enables male sterile line progenies to restore the male fertile property, wherein argopecten purpuratus and argopecten irradians irradians hybridized scallop belongs to hybrid progenies of argopecten purpuratus ovums and argopecten irradians irradians sperms. The construction method comprises the steps that excellent individuals in the male sterile line are selected, which are continuously backcrossed through different argopecten irradians irradians until all progenies are hermaphrodite, then the restoring line of the male sterile line is adopted, and finally the restoring line and corresponding male sterile line are subjected to hybridization, so that argopecten purpuratus and argopecten irradians irradians hybridized scallop product seedlings are produced. The method establishes a male sterile line restoring line in animals for the first time, and effectively overcomes the obstacle of autofertilization during hermaphrodite scallop hybridization, and the annual yield of the produced argopecten purpuratus and argopecten irradians irradians hybridized scallops is increased by 40 percent above compared with that of argopecten irradians irradians.

The invention discloses a method for cultivating a new variety of argopecten purpuratus and argopecten irradians concentricus hybrid scallops. The method comprises the following steps that S1, argopecten purpuratus and argopecten irradians concentricus scallops are utilized for colony hybridization, and a mixed colony including a parent selfing colony and a hybrid colony is obtained; S2, after shell color primary selection is carried out, hybrid individuals are identified with molecular biomarkers; S3, selfing purification of argopecten purpuratus and argopecten irradians concentricus hybrid F1 is carried out, wherein the identified hybrid individuals good in expression are selected, signal parent self sperms and eggs are subjected to selfing to set up a selfing family, a hybrid F2 familygood in expression is selected from the selfing family next year, at least three generations are continuously subjected to selfing breeding according to the same method, and the new variety of the argopecten purpuratus and argopecten irradians concentricus hybrid scallops good in character expression is obtained. The new variety of the character argopecten purpuratus and argopecten irradians concentricus hybrid scallops obtained through the method is high in combining ability, fast in growth, high in dressing percentage and bright in shell color, has good hybrid vigor, and can adapt to the high-temperature environment of the southern sea area in summer well.

The invention relates to a method for selectively breeding bay scallop, in particular to a contemporary multipoint composite breeding method for the bay scallop. Continuous shell length head-removed selection is carried out on scallops in different growth periods in the same generation of the bay scallop, the scallop with growth advantage is selected, and further contemporary multipoint compositebreeding is realized. The invention provides a scientific and effective bay scallop fast and efficient breeding method, scale of basic population used for breeding is expanded, reduction of genetic drift and genetic diversity caused by the fact that scale of a breeding group is small in a bay scallop breeding plan is avoided; meanwhile, a multi-time point continuous breeding method is used for improving breeding strength, breeding speed can be improved, and bay scallop breeding efficiency is improved.

The invention discloses a breeding method for scallop hybridization breeding. The breeding method is characterized by comprising the steps that firstly, gonad development synchronous breeding is carried out on selected chlamys farreri and Argopecten irradians; then, fully-developed female chlamys farreri in the chlamys farreri and the fully-developed Argopecten irradians are selected, after the female chlamys farreri finishes spawning, the hermaphroditic Argopectehs irradians is set for spermiation, and fertilization is carried out on sperms and eggs to form scallop hybridization fertilized eggs; incubation is carried out on the hybridization fertilized eggs, then larva selection is carried out, and larvas are bred in a larva breeding pond; finally the bred larvas are bred to scallop hybridization fry capable of being bred through settlement and metamorphosis. The hybridization scallop fry are bred from the female chlamys farreri and the Argopectehs irradias in a hybridization mode, and have the prominent advantages that in the breeding process, growing speed is high, resistance to diseases is high, the gonad is poor in fertility, the adductor muscle is large, the growing speed can be improved by more than 30 percent, the yield of primary products of the adductor muscle is improved by more than 40 percent, the obvious heterosis is achieved, and the sustainable development of scallop culture in China can be promoted.

The invention belongs to a shellfish breeding technology, and particularly relates to a 'northern breeding and southern raising' culture method in which hybrid scallops of argopecten purpuratus and argopecten irradians are bred in the north and then transferred to the south for raising to culture large-sized scallops. A new hybrid variety bred from the first-filial generation of argopecten purpuratus and argopecten irradians is used as seed scallops, scallop seedlings are bred in the north in early summer or autumn and then transported to a southern sea area to continue being cultured, and large-sized commercial scallops are obtained. The method can effectively increase the size of cultured scallops, increase the value of scallop products, effectively guarantee the product quality and safety of scallops, increase the production of high-quality marine protein and increase the income of fishermen, and provides a new path for the healthy and sustainable development of the scallop cultureindustry.

The invention discloses a method for inducing ocean seashell polyploidy by using sodium chloride. The method is characterized by comprising the following steps: selecting the seashell to grow the shell to respectively obtain sperms and ovum; carrying out artificial insemination; continuously observing the developing condition of zygotes through microscopic examination to determine the number of the water body for culturing the zygotes; adding sodium chloride into the culture sea water containing the zygotes according to a proportion that 20-40 go of sodium chloride is added in 1000ml of culture seat water when 40-50 percent of zygotes are observed to release a first polar body or a first zygote is observed to occur the first polar body, so as to prevent the release of a second polar body or the first polar body of the zygotes; evenly stirring and treating for 10-15 min; and transferring the zygotes to normal sea water to incubate . The ocean seashell comprises Pacific oyster, chlamys farreri, Argopecten irradians, Crassostrea rivularis, clam, Scapharca broughtonii and the like. The seashell zygotes are treated by using the sodium chloride and the initiating agent so as to inhibit the release of the polar body and produce the shell polyploidy with no toxicity, efficiency, simple operation and extremely low cost.

The invention relates to a construction method and application of the restorer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The restorer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line to restore the characteristic of male sterility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that excellent individuals in the male sterility line are selected, different varieties of Argopecten irradians concentricus are used for continuous backcross until all generations are hermaphrodite, and the restorer line of the male sterility line is obtained; finally, the restorer line and the corresponding male sterility line are used for hybridization to produce commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the restorer line of the male sterility liner is firstly built in animals, the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated, and the yield of the produced hybrid scallops of the purple scallops and the Argopecten irradians concentricus is improved by more than 42 percent relative to that of the Argopecten irradians concentricus in the same year.

The present invention relates to type G lysozyme, and is especially type G lysozyme gene of and its coded protein and cloning method. The type G lysozyme gene of gulf scallop has the base sequence shown in SEQ ID No. 1, and its coded protein has the amino acid sequence shown in SEQ ID No 2. The type G lysozyme gene of gulf scallop the present invention clones has polyadenyl acid tailing signal and polyadenyl acid tail, has important function in immunologic defence of gulf scallop, and may be used in the recombinant expression and gene transfer of lysozyme. The present invention lays foundation for the disease prevention and treatment, gene aided selective breeding and medicine development of gulf scallop.

The current invent relates to the construction and application of a three-line breeding system in the bay scallop×Peruvian scallop hybrids. The said three-line consists of a male sterile line, a maintainer line and a restorer line. A combination of male sterile line and maintainer line is obtained by continuously backcrossing the male sterile individuals selected from F1 inter-specific hybrid families with sperm of the selfing family of the sperm-providing Peruvian scallops until the progenies are all male sterile. The restorer line is obtained by continuously backcrossing selected individuals from the male sterile line with sperm of the selfing family of a Peruvian scallop until the progenies are all hermaphroditic and exhibit excellent production traits. Commercial male sterile brood stocks are produced by backcrossing the male sterile line with the maintainer line and commercial hybrid spats are produced by backcrossing the male sterile line and the restorer line.

The invention discloses a large-scale breeding method and equipment for hybrid young scallops. Through temperature regulation and other technical means, gonads of bay scallops and gonads of patinopecten yessoensis both of which become mature at the same time lay eggs and produce sperms respectively; then, a stepwise cooling mode is adopted for lowering the temperature of egg laying and spermiationwater for the bay scallops to be suitable for patinopecten yessoensis to lay the eggs, and then the egg laying and spermiation water for the bay scallops is mixed with the egg laying water for patinopecten yessoensis. The vitality of the sperms is maintained, the fertilization rate, hatching rate, survival rate and metamorphosis rate of planktonic larvae in a hybridization process are increased,and the production efficiency of the hybrid young scallops is improved. The hybrid young scallops also have the advantages of being high in growth speed, wide in temperature-resistant range, high in adult scallop yield and the like.

The invention relates to an establishment method and an application of a violet black hybrid scallop restorer. The restorer is a violet scallop system which enables the male sterile line offspring to restore the male fertile feature, wherein the violet black hybrid scallop is a hybrid offspring of Argopecten irradians concentricus eggs and violet scallop sperms. The establishment method comprises the steps of selecting excellent individuals in the male sterile line, continuously backcrossing different violet scallop sperms, and obtaining the restorer of the male sterile system until all offspring are hermaphrodite; and finally hybridizing the restorer with corresponding male sterile line to produce the violet black hybrid scallop commodity offspring seed. The male sterile restorer is firstly established in animals, the obstacle of autofertilization in the hybridization of the hermaphrodite scallops can be effectively overcome; the annual yield of the produced violet black hybrid scallop is improved by more than 45 percent than that of the Argopecten irradians concentricus.

The invention discloses an overwintering farming method of spotted maigre, which utilizes the idle phase of a shed after farming prawns and original facilitates and belongs to the field of agricultural seawater farming. An original cement pit, the shed and an oxygenation device of the farming shed are utilized; and the specific method comprises the following steps of: clearing pollutants after the end of farming, containing 60cm of water, disinfecting with 100ppm of bleaching powder, draining after 3 days, and air-drying for 3 days; enabling farming water to precipitate in a storage lagoon for 48 hours, disinfecting with the 100ppm of the bleaching powder, filtering by an 80-mesh silk cover after 3 days, pumping into the cement pit of the shed, and adding the water till the water level is 2.0m; when the temperature of the farming water of the spotted maigre is lower than 12 DEG C, transporting the spotted maigre into the shed for stocking, wherein the stocking density is 500kg / mu; and taking iced squid as baits, and feeding in fixed points in a timing and quantitative manner, wherein the feeding quantity is 1% of the total body weight of the farmed spotted maigre. Simultaneously, bay scallops are stocked in the pit according to the density of 100kg / mu, pollution discharge is performed once every 5 days, and 20cm of the water is replaced; and by adopting the method, the survival rate is above 98%, and the using method is simple, convenient, low in cost and easy to popularize.

The invention discloses a bay scallop breeding method. The bay scallop breeding method includes steps of 1) selecting a pond with sand substrate and disinfecting with water by bleaching powder or quick lime; 2) feeding water lower than 0.5 meter and adding fertilizer in ten days before juvenile scallops are placed into the pond, and feeding water to the highest level in two to three days before the juvenile scallops are placed into the pond; 3) setting a floating stem on a raft and fixing two ends of the floating stem by timber piles; 4) arranging a juvenile scallop curtain and utilizing a mesh bag with 60 meshes until the juvenile scallops bred to more than 400micrometers; 5) adding a mesh sheet into the mesh bag for supporting a juvenile scallop collecting bag; 6) gradually increasing daily water refreshment quantity in the second day after the juvenile scallops are placed into the pond; 7) keeping increasing water refreshment quantity within 3 days before dividing the scallops; 8) uniformly distributing the juvenile scallops on the mesh bag in two days after the juvenile scallops are subpacked, and moving the juvenile scallops to the sea and waiting for sales. By the above technical scheme, the bay scallop breeding method adopts scientific breeding technology, the bred scallops are fast in growth and fresh in meat, and the bay scallop breeding method is higher in output that other breeding methods.

The invention relates to a construction method and application of a male sterile line of argopecten irradians-argopecten purpuratus hybrid scallops. The male sterile line is a hybrid scallop line, wherein hybrid scallop line can not generate functional sperms, but the eggs can be fertilized and grow normally, progeny can inherit the male sterile characteristic, and the argopecten irradians-argopecten purpuratus hybrid scallops are the hybrid progeny of argopecten irradians eggs and argopecten purpuratus sperms. The construction method includes the steps that a great number of argopecten irradians-argopecten purpuratus hybrid first filial generation families are constructed, and male sterile individuals are found out from the argopecten irradians-argopecten purpuratus hybrid first filial generation families, then male parent argopecten purpuratus sperms of the male sterile individuals are used for continuous backcross selective breeding until all the backcross progeny are male sterile while the eggs are fertile, and the male sterile line of the argopecten irradians-argopecten purpuratus hybrid scallops can be obtained; the male sterile line and a maintainer line are hybridized to produce male sterile line parent scallops on a large scale, and the male sterile line parent scallops and a restorer line are hybridized to produce argopecten irradians-argopecten purpuratus hybrid scallop commodity seeds. The male sterile line of the scallops is set up in animals for the first time, and the obstacle to self-fertilization in hybridization of hermaphrodite scallops is effectively overcome.

The invention relates to a purple scallop ovum and bay scallop sperm hybrid scallop maintainer line construction method and application. According to a maintainer line, a corresponding purple scallop ovum and bay scallop sperm hybrid scallop male sterility line offspring is a bay scallop strain which is in male sterility and ovum fertility, and the purple scallop ovum and bay scallop sperm hybrid scallop is the hybrid offspring of the purple scallop ovum and the bay scallop sperm. The purple scallop ovum and bay scallop sperm hybrid scallop maintainer line construction method comprises the following steps of firstly, constructing a great quantity of first filial generation families of the purple scallop ovum and bay scallop sperm hybrid scallop, and finding a male sterility individual for breeding a male sterility line from the first filial generation families; then, continuously backcrossing by the male parent bay scallop sperm to respectively breed a male sterility line-maintainer line combination of which the offspring is completely the male sterility individual; finally, hybridizing to produce male sterility line parent scallop by the male sterility line and the maintainer line, wherein the male sterility line parent scallop is used for producing the offspring seed of the purple scallop ovum and bay scallop sperm hybrid scallop product. According to the invention, the scallop maintainer line is established in an animal for the first time, and therefore, autofertilization obstacles in hermaphroditic scallop hybrid are effectively overcome.

The invention belongs a breeding technology of shellfishes and particularly relates to a breeding method of cultivating hybridized scallops of purple scallops and bay scallops in the south and raisingthe hybridized scallops in the north in order to achieve large-scale cultivation of the scallops. According to the breeding method, the young hybridized scallops of the purple scallops and bay scallops are cultivated in the south during autumn and then transported to northern sea areas for follow-up raising in next spring. A new hybridized species selected from the first generation of the hybridized scallops of the purple scallops and bay scallops for breeding is adopted as the breeding scallops, the young scallops are cultivated in the south during autumn and then transported to the northernsea areas for follow-up raising, and therefore the commercial scallops with large specification are cultivated. Through establishment of a breeding pattern, the breeding time of the hybridized scallops of the purple scallops and bay scallops is prolonged, the breeding range is expanded, the specification of the bred scallops can be effectively increased, the value of scallop products is higher, and the quality and safety of the scallop products are effectively ensured. The yield of high-quality marine protein is increased, the income of fishermen is increased, and a new way to healthy sustainable development of the scallop breeding industry is created.

The invention relates to a building method and application of an Argopecten irradians irradians and Argopecten purpuratus hybrid scallop maintainer line. The maintainer line allows the corresponding male sterility line offspring of the corresponding Argopecten irradians irradians and Argopecten purpuratus hybrid scallop to have male sterile and egg fertile features. The Argopecten irradians irradians and Argopecten purpuratus hybrid scallop is the hybrid offspring of the eggs of the Argopecten irradians irradians and the sperms of the Argopecten purpuratus. The method includes: building a large amount of Argopecten irradians irradians and Argopecten purpuratus hybrid scallop first filial generation families, and finding male sterile individuals, for male sterility line breeding, in the families; using the sperms of male parent Argopecten purpuratus for continuous back crossing, and respectively breeding a male sterility line-maintainer line combination with all the offspring being male sterile individuals; using the male sterility line to hybridize with the retainer line to produce male sterility line parent scallops, and using the male sterility line parent scallop produce Argopecten irradians irradians and Argopecten purpuratus hybrid scallop commercial fry. The method has the advantages that the scallop retainer line is built in animals for the first time, and the barrier of self-fertilization of the hermaphrodite scallops during hybridization is overcome effectively.