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Method for extracting polysaccharide from Argopecten irradians

An extraction method and a technology for shellfish polysaccharide, applied in the field of food science, can solve the problems of affecting biological activity, time-consuming, and energy consumption, etc., and achieve the effects of high polysaccharide purity and activity, reasonable and effective extraction method, and improving polysaccharide purity.

Inactive Publication Date: 2012-05-30
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The defect of the above-mentioned method is: 1. The scallop tissue is untreated, not fully utilized, time-consuming, and energy-consuming
2. Ultrasonic vibration may cause a sharp rise in local temperature, which may destroy the polysaccharide structure and affect its biological activity
3. Although the enzyme extraction effect is good, the enzyme price is high, the versatility is poor, the cost is high, and it is not suitable for production

Method used

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Embodiment Construction

[0014] Shell fresh bay scallops, clean them, use a tissue masher to homogenate, dry the slurry in a blast drying oven at 50°C, crush it through an 80-mesh standard sampling sieve, soak it in 80% ethanol for 2-4 hours, remove Oligosaccharides and pigments are filtered and dried to obtain bay scallop powder. Mix bay scallop powder with 40 times volume of distilled water, extract at 90° C. for 6 hours, and cool to obtain bay scallop polysaccharide aqueous extract. Centrifuge the bay scallop polysaccharide aqueous extract at 4000rmp for 20min, collect the supernatant and filter the polysaccharide mother liquor. The obtained bay scallop polysaccharide mother liquor is vacuum rotary evaporated, the temperature is below 60° C., and concentrated to 1 / 3 of the original volume to obtain bay scallop concentrate. Slowly add ethanol with a concentration of 95% to the concentrated solution to make the alcohol content reach 75%, place it in the refrigerator (4°C-8°C) overnight, a precipitat...

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Abstract

A method for extracting polysaccharide from Argopecten irradians comprises processing raw material, extracting with water, separating, concentrating, precipitating with ethanol and drying, which belongs to the technical field of food science. Specifically, the method comprises pounding Argopecten irradians tissue, oven drying or freeze drying, soaking in a reagent, and refluxing for removing oligosaccharide and pigment; extracting with water as a solvent; centrifuging to obtain a supernatant; performing vacuum rotary evaporation and concentration; adding anhydrous ethanol into the obtained concentrated solution for precipitating polysaccharide when the ethanol reaches a certain of concentration; and drying. The inventive method has high yield. The obtained product has high purity, is easy for further separation and purification, and can be directly used as a product or be processed into tablets and capsules. The invention is of far reaching importance to high-value processing of Argopecten irradians and development of functional food.

Description

technical field [0001] The invention relates to polysaccharide extraction technology, in particular to the technology of extracting polysaccharide from shellfish, which belongs to the field of food science and technology. Background technique [0002] Polysaccharides are widely distributed in almost all organisms such as animals, plants, microorganisms, seaweeds, etc. Aging, enhance the immune function of the body. In recent years, the research on shellfish polysaccharides has confirmed that it has various biological activities such as anti-tumor, anti-oxidation, lowering blood sugar, and lowering blood lipids. At present, it has been isolated from various marine organisms at home and abroad. Active polysaccharide components, Yin Hongling and others studied the extraction of polysaccharides from the viscera of Ezo scallop and the effect of scavenging hydroxyl free radicals. The publication number is CN1749281, and the Chinese patent application with the application number 2...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张志胜佟海菊赵丛枝孙克岩刘亚南宋欣
Owner HEBEI AGRICULTURAL UNIV.
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