38 results about "Argopecten purpuratus" patented technology

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossed scallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparison with the current yield of Argopecten irradians.

The invention relates to a method for cultivating hybridized and backcrossed marine product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten purpuratus and Argopecten irradians, and synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten irradians; inducing the hybridized first filial generation individuals to spawn by using a conventional shade drying and temperature stimulation method so as to obtain unfertilized eggs; simultaneously, stimulating the Argopecten irradians to spawn and discharge seminal fluid, and filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten irradians; and fertilizing the hybridized first filial generation eggs with the seminal fluid of the Argopecten irradians, cultivating according to a conventional method after hatching germ cells, and thus obtaining the hybridized and backcrossed marine product fries which are above 1.5 cm in shell height and used for commercial cultivation. The hybridized and backcrossed marine product fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed marine product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed marine product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

The invention relates to a method for cultivating hybridized and backcrossed Argopecten product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten irradians and Argopecten purpuratus; synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten purpuratus; inducing the hybridized first filial generation individuals to ovulate by using a conventional shade drying and temperature stimulation method; simultaneously, stimulating the Argopecten purpuratus to spawn and discharge seminal fluid; filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten purpuratus; fertilizing the seminal fluid of the Argopecten purpuratus with the hybridized first filial generation eggs; carrying out larva cultivation and fry intermediate cultivation according to a conventional method after hatching; and thus obtaining the hybridized and backcrossed Argopecten product fries having obvious heterosis. The hybridized and backcrossed marine Argopecten fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed Argopecten product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed Argopecten product fries can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

The invention relates to a construction method and application of the maintainer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The maintainer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus to have the characteristics of male sterility and ovum fertility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that firstly, a large number of the first filial generations of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are constructed, and male sterile individuals are found out from the first filial generations for breeding of the male sterility line; then sperms of male parent Argopecten irradians concentricus are used for continuous backcross, and the combinations of the male sterility line-maintainer line with all generations of male sterility individuals are respectively bred; finally, the male sterility line parent scallops are produced through hybridization of the male sterility line and the maintainer line, and the maintainer line is used for producing commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the maintainer line of the scallops is firstly built in animals, and the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated.

The invention relates to a selective breeding method for a hybridized-Argopecten irradians backcross low-temperature-resistant scallop line. The method is characterized by comprising the steps: firstly, selecting individuals, which are high in growth speed and high in low-temperature resistance, from a family or population of a first filial generation of Argopecten irradians and Argopecten purpuratus as parent scallops; carrying out back crossing on individuals, with high low-temperature resistance, of the first filial generation and Argopecten irradians ova so as to establish a backcross family or population; and then, carrying out selective breeding on low-temperature resistant breeding core groups for at least three times. According to the method, the first filial generation which is high in growth speed and remarkable in heterosis is adopted as a male parent and then is subjected to back crossing with the Argopecten irradians (a female parent) so as to polymerize a chilling-resistant gene of the Argopecten irradians to a first generation of hybridized-Argopecten irradians backcross, the individuals, with high low-temperature resistance, of the first generation of hybridized-Argopecten irradians backcross are taken as a breeding core group and is subjected to continuous 3 generations of low-temperature resistant breeding, the low-temperature resistance is extended to 0 DEG C, the growth speed is increased by 15% compared with that of the Argopecten irradians, and then, a low-temperature resistant new line which is applicable to wintering of Shandong sea area is obtained. Apparently, the method is simple and convenient in operation and is extensively applied to the selective breeding of hybridized low-temperature resistant scallop lines.

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossedscallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparisonwith the current yield of Argopecten irradians.

The invention relates to a method for cultivating hybridized and backcrossed marine product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten purpuratus and Argopecten irradians, and synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten irradians; inducing the hybridized first filial generation individuals to spawn by using a conventional shade drying and temperature stimulation method so as to obtain unfertilized eggs; simultaneously, stimulating the Argopecten irradians to spawn and discharge seminal fluid, and filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten irradians; and fertilizing the hybridized first filial generation eggs with the seminal fluid of the Argopecten irradians, cultivating according to a conventional method after hatching germcells, and thus obtaining the hybridized and backcrossed marine product fries which are above 1.5 cm in shell height and used for commercial cultivation. The hybridized and backcrossed marine productfries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed marine product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed marine product fries canbe cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention relates to a method for breeding low-temperature-tolerant strain of Argopecten irradias hybrid-backcrossed scallops, characterized by including: selecting individuals that are high in growth speed and tolerance to low temperatures from Argopecten irradias and Argopecten purpuratus hybrid first-generation family line or population as breed scallops; backcrossing the individuals having high tolerance to low temperatures in the hybrid first generation with semen of Argopecten irradias to establish a backcrossed family line or population; finally, performing breeding of low-temperature-tolerant breeding core population at least three times to develop a low-temperature-tolerant strain. The Argopecten irradias and Argopecten purpuratus hybrid first-generation high in growth speed and significant in hybridization advantage is used as a female parent that is backcrossed with Argopecten irradias (male parent) to gather cold-tolerant genes of Argopecten irradias to the Argopecten irradias hybrid backcrossed first-generation, the individuals having high tolerance to low temperatures in the Argopecten irradias hybrid backcrossed first-generation are used as a breeding core population, the breed core population is subjected to continuous three generations of low-temperature-tolerant breeding, and the strain can tolerate a low temperature down to 0 DEG C and grows 15% higher than Argopecten irradias. It is apparent that the method is simple and convenient to perform and is widely applicable to the breeding of low-temperature-tolerant strains of hybrid scallops.

The invention relates to a method for cultivating hybridized and backcrossed Argopecten product fries. The method is characterized by comprising the following steps of: selecting excellent individuals that only a female area grows in a hybridized first filial generation family or group of Argopecten irradians and Argopecten purpuratus; synchronously maturing the selected excellent individuals with excellent individuals of the Argopecten purpuratus; inducing the hybridized first filial generation individuals to ovulate by using a conventional shade drying and temperature stimulation method; simultaneously, stimulating the Argopecten purpuratus to spawn and discharge seminal fluid; filtering through 500-mesh bolting cloth to obtain the seminal fluid of the Argopecten purpuratus; fertilizingthe seminal fluid of the Argopecten purpuratus with the hybridized first filial generation eggs; carrying out larva cultivation and fry intermediate cultivation according to a conventional method after hatching; and thus obtaining the hybridized and backcrossed Argopecten product fries having obvious heterosis. The hybridized and backcrossed marine Argopecten fries cultivated by using the method provided by the invention simultaneously have the characteristics of the Argopecten irradians and the Argopecten purpuratus; compared with the Argopecten purpuratus, the hybridized and backcrossed Argopecten product fries have obvious advantages on growth, individual size and temperature tolerance, therefore, the hybridized and backcrossed Argopecten product fries can be cultivated in sea areas inthe north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 50%.

The invention relates to a construction method and application of an argopecten purpuratus and argopecten irradians irradians hybridized scallop restoring line. The restoring line belongs to an argopecten irradians irradians strain which enables male sterile line progenies to restore the male fertile property, wherein argopecten purpuratus and argopecten irradians irradians hybridized scallop belongs to hybrid progenies of argopecten purpuratus ovums and argopecten irradians irradians sperms. The construction method comprises the steps that excellent individuals in the male sterile line are selected, which are continuously backcrossed through different argopecten irradians irradians until all progenies are hermaphrodite, then the restoring line of the male sterile line is adopted, and finally the restoring line and corresponding male sterile line are subjected to hybridization, so that argopecten purpuratus and argopecten irradians irradians hybridized scallop product seedlings are produced. The method establishes a male sterile line restoring line in animals for the first time, and effectively overcomes the obstacle of autofertilization during hermaphrodite scallop hybridization, and the annual yield of the produced argopecten purpuratus and argopecten irradians irradians hybridized scallops is increased by 40 percent above compared with that of argopecten irradians irradians.

The invention discloses a method for cultivating a new variety of argopecten purpuratus and argopecten irradians concentricus hybrid scallops. The method comprises the following steps that S1, argopecten purpuratus and argopecten irradians concentricus scallops are utilized for colony hybridization, and a mixed colony including a parent selfing colony and a hybrid colony is obtained; S2, after shell color primary selection is carried out, hybrid individuals are identified with molecular biomarkers; S3, selfing purification of argopecten purpuratus and argopecten irradians concentricus hybrid F1 is carried out, wherein the identified hybrid individuals good in expression are selected, signal parent self sperms and eggs are subjected to selfing to set up a selfing family, a hybrid F2 familygood in expression is selected from the selfing family next year, at least three generations are continuously subjected to selfing breeding according to the same method, and the new variety of the argopecten purpuratus and argopecten irradians concentricus hybrid scallops good in character expression is obtained. The new variety of the character argopecten purpuratus and argopecten irradians concentricus hybrid scallops obtained through the method is high in combining ability, fast in growth, high in dressing percentage and bright in shell color, has good hybrid vigor, and can adapt to the high-temperature environment of the southern sea area in summer well.

The invention belongs to a shellfish breeding technology, and particularly relates to a 'northern breeding and southern raising' culture method in which hybrid scallops of argopecten purpuratus and argopecten irradians are bred in the north and then transferred to the south for raising to culture large-sized scallops. A new hybrid variety bred from the first-filial generation of argopecten purpuratus and argopecten irradians is used as seed scallops, scallop seedlings are bred in the north in early summer or autumn and then transported to a southern sea area to continue being cultured, and large-sized commercial scallops are obtained. The method can effectively increase the size of cultured scallops, increase the value of scallop products, effectively guarantee the product quality and safety of scallops, increase the production of high-quality marine protein and increase the income of fishermen, and provides a new path for the healthy and sustainable development of the scallop cultureindustry.

The invention relates to a construction method and application of a male sterile line of argopecten irradians-argopecten purpuratus hybrid scallops. The male sterile line is a hybrid scallop line, wherein hybrid scallop line can not generate functional sperms, but the eggs can be fertilized and grow normally, progeny can inherit the male sterile characteristic, and the argopecten irradians-argopecten purpuratus hybrid scallops are the hybrid progeny of argopecten irradians eggs and argopecten purpuratus sperms. The construction method includes the steps that a great number of argopecten irradians-argopecten purpuratus hybrid first filial generation families are constructed, and male sterile individuals are found out from the argopecten irradians-argopecten purpuratus hybrid first filial generation families, then male parent argopecten purpuratus sperms of the male sterile individuals are used for continuous backcross selective breeding until all the backcross progeny are male sterile while the eggs are fertile, and the male sterile line of the argopecten irradians-argopecten purpuratus hybrid scallops can be obtained; the male sterile line and a maintainer line are hybridized to produce male sterile line parent scallops on a large scale, and the male sterile line parent scallops and a restorer line are hybridized to produce argopecten irradians-argopecten purpuratus hybrid scallop commodity seeds. The male sterile line of the scallops is set up in animals for the first time, and the obstacle to self-fertilization in hybridization of hermaphrodite scallops is effectively overcome.

The invention relates to a building method and application of an Argopecten irradians irradians and Argopecten purpuratus hybrid scallop maintainer line. The maintainer line allows the corresponding male sterility line offspring of the corresponding Argopecten irradians irradians and Argopecten purpuratus hybrid scallop to have male sterile and egg fertile features. The Argopecten irradians irradians and Argopecten purpuratus hybrid scallop is the hybrid offspring of the eggs of the Argopecten irradians irradians and the sperms of the Argopecten purpuratus. The method includes: building a large amount of Argopecten irradians irradians and Argopecten purpuratus hybrid scallop first filial generation families, and finding male sterile individuals, for male sterility line breeding, in the families; using the sperms of male parent Argopecten purpuratus for continuous back crossing, and respectively breeding a male sterility line-maintainer line combination with all the offspring being male sterile individuals; using the male sterility line to hybridize with the retainer line to produce male sterility line parent scallops, and using the male sterility line parent scallop produce Argopecten irradians irradians and Argopecten purpuratus hybrid scallop commercial fry. The method has the advantages that the scallop retainer line is built in animals for the first time, and the barrier of self-fertilization of the hermaphrodite scallops during hybridization is overcome effectively.

The invention relates to the technical field of marine economic shellfish cross breeding, in particular to a breeding method based on argopecten purpuratus, argopecten irradians and Argopecten irradians concentricus three-way hybrid scallop commodity fries. The argopecten irradians and the Argopecten irradians concentricus are subjected to intersubspecific hybridization, then the obtained first-generation scallops of the intersubspecific hybridization and the argopecten purpuratus are used as seed scallops and are respectively subjected to high selfing purification, and the obtained purified sea and ink sea (or ink sea) hybrid scallops and the argopecten purpuratus are subjected to interspecific hybridization; therefore, large-scale preparation of the sea-black-black-purple (or black-sea-black-purple) three-way hybrid scallop commodity offspring seeds is realized. According to the method for preparing the three-way hybrid scallop commodity offspring seeds of the Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus, Argopecten purpuratus,

The invention relates to a construction method and application of a male sterility line of Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops. The male sterility line is a hybridization scallop strain according to which functional sperms cannot be generated, eggs of the male sterility line can be fertilized and grow normally and a male sterility characteristic of the male sterility line can be entailed on offsprings. The Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops are a filial generation between Argopecten irradians concentricus eggs and Argopecten purpuratus sperms. The method comprises the steps that first a large number of first filial generation families obtained after hybridization between Argopecten irradians concentricus and Argopecten purpuratus are constructed, and male sterile individuals are found among the first filial generation families obtained after hybridization between Argopecten irradians concentricus and Argopecten purpuratus; second, sperms of male parent Argopecten purpuratus of the male sterile individuals are used for continuous backcross for seed selection till backcross offsprings are all male and sterile and have fertile eggs, and the male sterility line of the Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops is obtained; third, male sterility line parent scallops are produced on a large scale through hybridization between the male sterility line and a maintainer line, and Argopecten irradians concentricus and Argopecten purpuratus hybridization scallop commercial offspring seeds are produced on a large scale through hybridization between the male sterility line parent scallops and a restorer line. According to the construction method and application of the male sterility line of the Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops, the male sterility line of scallops is constructed among animals for the first time, and the obstacle in self-fertilization of hybridization of hermaphrodite scallops is effectively removed.

The present invention relates to a chimeric nucleotide sequence encoding peptides with antimicrobial activity, to be expressed on plants as bioreactors, plant cell or transformed plant material and E. coli, where plants and bacteria were used for scale-up production of antimicrobial peptide and that produces the peptide sequence derived from SEQ ID No. 1, SEQ ID No. 6 and SEQ ID No. 12. It includes a method for obtaining the amino acids sequence SEQ ID No. 9 derived from a chimeric nucleotide sequence which encodes to antimicrobial peptides, wherein said amino acids sequence is derived from the synthetic DNA sequence SEQ ID No. 1, obtained from a back-translation of the amino acid sequence of the peptide Ap-S of Argopecten purpuratus scallop.

The invention relates to the technical field of cross breeding of marine economic shellfish, in particular to a breeding method based on three-way cross of argopecten purpuratus, argopecten irradians and Argopecten irradians concentricus. The method comprises the following steps: firstly, carrying out intersubspecific hybridization on argopecten irradians and argopecten irradians concentricus to obtain first filial generation of hybridized scallops of which the fertility is not influenced; performing interspecific hybridization on the obtained first filial generation of scallops and argopecten purpuratus to obtain the first filial generation of interspecific hybridization scallops which normally develop and have remarkable growth and stress resistance advantages, and purifying the three-way filial generation through group mixing; and the new species of the sea-black-black-purple (or black-black-black-black-purple) three-way hybrid scallops with genetic stability and excellent characters can be obtained through continuous breeding. According to the method disclosed by the invention, the excellent characters of the argopecten purpuratus, the argopecten irradians and the Argopecten irradians concentricus can be integrated, so that a new three-way hybrid scallop variety with more remarkable growth and stress resistance advantages is cultivated, and the improved variety preservation and supply capability of the scallop breeding industry is improved.

The invention relates to a selective breeding method for a hybridized-Argopecten irradians backcross low-temperature-resistant scallop line. The method is characterized by comprising the steps: firstly, selecting individuals, which are high in growth speed and high in low-temperature resistance, from a family or population of a first filial generation of Argopecten irradians and Argopecten purpuratus as parent scallops; carrying out back crossing on individuals, with high low-temperature resistance, of the first filial generation and Argopecten irradians ova so as to establish a backcross family or population; and then, carrying out selective breeding on low-temperature resistant breeding core groups for at least three times. According to the method, the first filial generation which is high in growth speed and remarkable in heterosis is adopted as a male parent and then is subjected to back crossing with the Argopecten irradians (a female parent) so as to polymerize a chilling-resistant gene of the Argopecten irradians to a first generation of hybridized-Argopecten irradians backcross, the individuals, with high low-temperature resistance, of the first generation of hybridized-Argopecten irradians backcross are taken as a breeding core group and is subjected to continuous 3 generations of low-temperature resistant breeding, the low-temperature resistance is extended to 0 DEG C, the growth speed is increased by 15% compared with that of the Argopecten irradians, and then, a low-temperature resistant new line which is applicable to wintering of Shandong sea area is obtained. Apparently, the method is simple and convenient in operation and is extensively applied to the selective breeding of hybridized low-temperature resistant scallop lines.

The invention discloses a method for cultivating a new variety of argopecten purpuratus and argopecten irradians concentricus hybrid scallops. The method comprises the following steps that S1, argopecten purpuratus and argopecten irradians concentricus scallops are utilized for colony hybridization, and a mixed colony including a parent selfing colony and a hybrid colony is obtained; S2, after shell color primary selection is carried out, hybrid individuals are identified with molecular biomarkers; S3, selfing purification of argopecten purpuratus and argopecten irradians concentricus hybrid F1 is carried out, wherein the identified hybrid individuals good in expression are selected, signal parent self sperms and eggs are subjected to selfing to set up a selfing family, a hybrid F2 familygood in expression is selected from the selfing family next year, at least three generations are continuously subjected to selfing breeding according to the same method, and the new variety of the argopecten purpuratus and argopecten irradians concentricus hybrid scallops good in character expression is obtained. The new variety of the character argopecten purpuratus and argopecten irradians concentricus hybrid scallops obtained through the method is high in combining ability, fast in growth, high in dressing percentage and bright in shell color, has good hybrid vigor, and can adapt to the high-temperature environment of the southern sea area in summer well.

The invention relates to a construction method and application of an argopecten irradians-argopecten purpuratus hybrid scallop restorer line. The restorer line is argopecten purpuratus which can recover the male fertile characteristic for male sterile line progeny, wherein argopecten irradians-argopecten purpuratus hybrid scallops are hybrid progeny of argopecten irradians eggs and argopecten purpuratus sperms. The construction method includes the steps that excellent individuals in a male sterile line are selected, the different argopecten purpuratus sperms are used for continuous backcross until the progeny are all hermaphrodite, and the restorer line of the male sterile line is obtained; finally, the restorer line is hybridized with the corresponding male sterile line of the argopecten irradians-argopecten purpuratus hybrid scallops to produce argopecten irradians-argopecten purpuratus hybrid scallop commodity seeds. The restorer line of the male sterile line is set up in animals for the first time, the obstacle to self-fertilization in hybridization of hermaphrodite scallops is effectively overcome, and the yield of the produced argopecten irradians-argopecten purpuratus hybrid scallops in the current year is larger than that of argopecten irradians and is increased by over 50%.

The invention relates to a construction method and application of a male sterile line of argopecten irradians-argopecten purpuratus hybrid scallops. The male sterile line is a hybrid scallop line, wherein hybrid scallop line can not generate functional sperms, but the eggs can be fertilized and grow normally, progeny can inherit the male sterile characteristic, and the argopecten irradians-argopecten purpuratus hybrid scallops are the hybrid progeny of argopecten irradians eggs and argopecten purpuratus sperms. The construction method includes the steps that a great number of argopecten irradians-argopecten purpuratus hybrid first filial generation families are constructed, and male sterile individuals are found out from the argopecten irradians-argopecten purpuratus hybrid first filial generation families, then male parent argopecten purpuratus sperms of the male sterile individuals are used for continuous backcross selective breeding until all the backcross progeny are male sterile while the eggs are fertile, and the male sterile line of the argopecten irradians-argopecten purpuratus hybrid scallops can be obtained; the male sterile line and a maintainer line are hybridized to produce male sterile line parent scallops on a large scale, and the male sterile line parent scallops and a restorer line are hybridized to produce argopecten irradians-argopecten purpuratus hybrid scallop commodity seeds. The male sterile line of the scallops is set up in animals for the first time, and the obstacle to self-fertilization in hybridization of hermaphrodite scallops is effectively overcome.

The invention belongs to the cultivation technology of shellfish, and in particular relates to a cultivation method of "north breeding and south cultivation" in which purple sea hybrid scallops are raised in the north and then moved to the south for cultivation to cultivate large-scale scallops. The new hybrid species selected from the first generation of hybrids of purple scallops and bay scallops is used as seed shells, and scallop seedlings are cultivated in the north in early summer or autumn, and then transported to the southern waters for further breeding, and large-scale commercial scallops are cultivated. The invention can effectively improve the specifications of cultured scallops, increase the value of scallop products, effectively ensure the product quality and safety of scallops, increase the production of high-quality marine protein, increase the income of fishermen, and open up a new path for the healthy and sustainable development of scallop farming. the way.

The invention relates to a construction method and application of a argopecten irradians concentricus and argopecten purpuratus hybrid scallop maintenance line. The maintenance line can enable corresponding male-sterile line offsprings of argopecten irradians concentricus and argopecten purpuratus hybrid scallops to still have argopecten purpuratus lines with the characteristics of male sterility and egg fertility, wherein the argopecten irradians concentricus and argopecten purpuratus hybrid scallops are the hybrid offsprings of argopecten irradians concentricus eggs and argopecten purpuratus sperms. The construction method comprises the following steps of firstly, constructing lots of argopecten irradians concentricus and argopecten purpuratus hybrid scallop generation lines, and finding male-sterile individuals for select breeding a male-sterile line; secondly, using sperms of the male parent argopecten purpuratus scallops to continuous back cross, and respectively select breeding the offsprings to be male-sterile line-maintenance line combination of the male-sterile individuals; finally, hybridizing the male-sterile line and the maintenance line to produce male-sterile line scallops, and using the maintenance line to produce argopecten irradians concentricus and argopecten purpuratus hybrid scallop commercial fingerlings. According to the construction method and the application of the argopecten irradians concentricus and argopecten purpuratus scallop maintenance line, the maintenance line of the scallops is constructed in animals for the first time, and the obstacle of autofertilization in hermaphrodite scallop hybridization is effectively overcome.

The invention relates to a construction method and application of an argopecten purpuratus and argopecten irradians irradians hybridized scallop restoring line. The restoring line belongs to an argopecten irradians irradians strain which enables male sterile line progenies to restore the male fertile property, wherein argopecten purpuratus and argopecten irradians irradians hybridized scallop belongs to hybrid progenies of argopecten purpuratus ovums and argopecten irradians irradians sperms. The construction method comprises the steps that excellent individuals in the male sterile line are selected, which are continuously backcrossed through different argopecten irradians irradians until all progenies are hermaphrodite, then the restoring line of the male sterile line is adopted, and finally the restoring line and corresponding male sterile line are subjected to hybridization, so that argopecten purpuratus and argopecten irradians irradians hybridized scallop product seedlings are produced. The method establishes a male sterile line restoring line in animals for the first time, and effectively overcomes the obstacle of autofertilization during hermaphrodite scallop hybridization, and the annual yield of the produced argopecten purpuratus and argopecten irradians irradians hybridized scallops is increased by 40 percent above compared with that of argopecten irradians irradians.

The invention relates to a construction method and application of a male sterility line of Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops. The male sterility line is a hybridization scallop strain according to which functional sperms cannot be generated, eggs of the male sterility line can be fertilized and grow normally and a male sterility characteristic of the male sterility line can be entailed on offsprings. The Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops are a filial generation between Argopecten irradians concentricus eggs and Argopecten purpuratus sperms. The method comprises the steps that first a large number of first filial generation families obtained after hybridization between Argopecten irradians concentricus and Argopecten purpuratus are constructed, and male sterile individuals are found among the first filial generation families obtained after hybridization between Argopecten irradians concentricus and Argopecten purpuratus; second, sperms of male parent Argopecten purpuratus of the male sterile individuals are used for continuous backcross for seed selection till backcross offsprings are all male and sterile and have fertile eggs, and the male sterility line of the Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops is obtained; third, male sterility line parent scallops are produced on a large scale through hybridization between the male sterility line and a maintainer line, and Argopecten irradians concentricus and Argopecten purpuratus hybridization scallop commercial offspring seeds are produced on a large scale through hybridization between the male sterility line parent scallops and a restorer line. According to the construction method and application of the male sterility line of the Argopecten irradians concentricus and Argopecten purpuratus hybridization scallops, the male sterility line of scallops is constructed among animals for the first time, and the obstacle in self-fertilization of hybridization of hermaphrodite scallops is effectively removed.