Construction method and application of male sterile line of argopecten irradians-argopecten purpuratus hybrid scallops

A male sterile line, male sterility technology, applied in application, climate change adaptation, fish farming, etc., to overcome the obstacles of self-fertilization and low cost

Active Publication Date: 2014-12-10
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to realize the construction method of the male sterile line of Haizi hybrid scallop in the hermaphroditic scallop specifically in animal breeding, to overcome the deficiencies in the existing hybrid scallop breeding technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1) Screening of male sterile individuals of sea purple hybrid scallops and breeding of their male parents: select 50 sea scallops with mature gonads and 50 purple scallops in the first year, and place each scallop in the pre-added Spawn eggs and ejaculate in a separate container in seawater at 23°C, collect the ova of each bay scallop and the sperm and eggs of each purple scallop separately, add a small amount from a single purple scallop to the egg suspension of each bay scallop Stir to mix the sperm and eggs evenly, observe under a microscope so that there are 5-6 sperm around each egg, and place the fertilized eggs in a dark environment to hatch, that is, to obtain the first-generation family of Haizi hybrids. Establish at least 20 such hybrid families with the above method, and simultaneously fertilize the fertilized purple scallop sperm eggs to establish a corresponding number of purple scallop self-bred families, and carry out larvae cultivation, seedling preservat...

Embodiment 2

[0029] At the end of March 2008, the bay scallops with mature gonads were taken, and the eggs of bay scallops were obtained after temperature stimulation. After insemination with the sperm of purple scallops, the first-generation seedlings of hybrids of bay scallops and purple scallops were obtained and raised. In the spring of 2009, the first-generation scallops of the above-mentioned hybrids were matured, and the scallops that were large and only developed in the female area were selected from them. After 30 minutes of drying in the shade, the scallops were placed in a container filled with seawater at 23°C to stimulate spawning. The sperm of the scallop was mixed and fertilized, and the backcross population of the sea purple hybrid scallop and the purple scallop was bred. Most of the backcross populations were hermaphroditic after maturation in the spring of 2010, but <10% of the individuals were still male sterile.

[0030] Continue to backcross the above-mentioned male st...

Embodiment 3

[0033] In 2012, 20 male sterile individuals in the first-generation family of Haizi hybrids were stimulated to lay eggs. These eggs were backcrossed with the sperm of a single purple scallop, and 2 were successfully obtained. When the backcrossing families were harvested in mid-November 2012, compared with the bay scallops in the same period, the shell height, shell length, shell width and weight of one of the backcrossing families increased by 28.2%, 29.5%, 16.4% and 74.0%, respectively. After ripening in the spring of 2013, it was found that most of the offspring only developed in the female area, which proved that the male sterile line of Haizi hybrid scallop can be bred by backcrossing with purple scallop sperm and male sterile individuals.

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Abstract

The invention relates to a construction method and application of a male sterile line of argopecten irradians-argopecten purpuratus hybrid scallops. The male sterile line is a hybrid scallop line, wherein hybrid scallop line can not generate functional sperms, but the eggs can be fertilized and grow normally, progeny can inherit the male sterile characteristic, and the argopecten irradians-argopecten purpuratus hybrid scallops are the hybrid progeny of argopecten irradians eggs and argopecten purpuratus sperms. The construction method includes the steps that a great number of argopecten irradians-argopecten purpuratus hybrid first filial generation families are constructed, and male sterile individuals are found out from the argopecten irradians-argopecten purpuratus hybrid first filial generation families, then male parent argopecten purpuratus sperms of the male sterile individuals are used for continuous backcross selective breeding until all the backcross progeny are male sterile while the eggs are fertile, and the male sterile line of the argopecten irradians-argopecten purpuratus hybrid scallops can be obtained; the male sterile line and a maintainer line are hybridized to produce male sterile line parent scallops on a large scale, and the male sterile line parent scallops and a restorer line are hybridized to produce argopecten irradians-argopecten purpuratus hybrid scallop commodity seeds. The male sterile line of the scallops is set up in animals for the first time, and the obstacle to self-fertilization in hybridization of hermaphrodite scallops is effectively overcome.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to a construction method and application of a male sterile line of Haizi hybrid scallop (the hybrid offspring of bay scallop ova and purple scallop sperm). Background technique [0002] The bay scallop (Argopecten irradians irradians) is native to the Atlantic coast of the United States and was introduced to my country in 1982. The bay scallop grows fast, has a wide range of temperature adaptability, and has strong stress resistance. It is very suitable for farming in the northern seas of my country. At present, the output and farming area account for more than 50% of the scallop farming in my country. However, due to the long-term inbreeding after the introduction, the germplasm of bay scallops has been seriously degraded during the breeding process. The size of commercial shellfish is getting smaller and small...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00A01K61/54
CPCY02A40/81
Inventor 王春德马斌孙奎府赵玉明刘博周顺王文琪赵侠苗国英
Owner QINGDAO AGRI UNIV
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