172results about How to "Individual big" patented technology

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossed scallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparison with the current yield of Argopecten irradians.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

Disclosed is a process for preparing microbiologically active organic fertilizer by utilizing discarded mushroom residue, which comprises the following steps, disintegrating the discarded mushroom residue, mixing with disintegrated stalks of agronomic crops or leftover bits and pieces of food processing factories, moistening with water, making the water content of the material to be about 65-70%, inoculating 2-8% of enzyme production composite microorganism agent, placing them in a fermenting tank, sealing for fermentation 5-15 days at a predetermined temperature, ventilating 15 minutes for every 24 hours during the fermentation process, when the biomass and enzyme vitality of the microorganism reach index point, the microbiologically active organic fertilizer can be obtained. The invention solves the problem of environmental pollution existed in the conventional treatment process for discarded mushroom residue.

The invention discloses a specific antibody of major royal jelly protein MRJP1 and a preparation method thereof and Elisa quantitative detection thereof. First, homology analysis is performed to amino acid sequences of proteins of all members of the Apismellifera major royal jelly protein MRJPs family (MRJP1-MRJP9) to select a specific polypeptide amino acid sequence unlike other MRJPs family members in MRJP1. The related specific polypeptide is synthesized by using a chemical method, and is used as an antigen to immunize New Zealand white rabbits; taking serum, and performing Elisa assay obtaining polyclonal antibody R2 with a relatively high titer, then purifying the antibody by using an affinity column prepared from the synthesized MRJP1 polypeptide. The titer of antibody R2 is detected via Elisa assay by using MRJP1 as the antigen, and the titer of the antibody is greater than 1:20000. The present invention provides a very reliable new rapid detection method for the qualitative and quantitative detection of MRJP1 in royal jelly, and also provides a very reliable technical means for quality control, freshness detection, and identification of genuine products of royal jelly and honey products for bee product quality supervision departments and processing and trading enterprises.

Cultured Penaeus vannamei Boone has the advantages of high growth speed, strong adaptability and high yield and becomes one of the main cultured varieties in northern areas of China. (1) pond water color control: the ideal color of water for culturing the Penaeus vannamei Boone is yellow green or yellow brown formed by green algae or diatoms; the algae or diatoms are benign biocenosis in the micro-ecological environment of a pond and play a role of purifying water quality; (2) control of pond water pH value, dissolved oxygen and transparency: the pH value suitable for the Penaeus vannamei Boone is 7.8-8.5, but the peak value of 9.2 is generated at the middle and final stages culturing, and therefore, the pH value control is especially focused in the culturing process; the pH value is not suitable to be high, otherwise, the toxicity of ammonia nitrogen can be increased, thereby restraining the growth of the Penaeus vannamei Boone; (3) scientific bait casting: generally, low-cost iced fish cream or small shells are fed, and some formula feeds are also fed; the formula feeds can be manually compounded; the formula feeds contain the following components: 17 percent of fish meal, 40 percent of soya-bean cake, 28 percent of bran, 10 percent of wheat middling, 3 percent of bone meal and 2 percent of additive; and (4) pond patrol: the pond is patrolled in the morning, at night and in the midnight every day to observe the water color change and whether Penaeus vannamei Boone raise above water to breathe or not; the activity condition, the growth condition and the repletion rate of the Penaeus vannamei Boone are observed by adopting two methods of lamplight observing and spoon-net inspection so as to regulate the bait casting amount and decide whether to start an oxygen-enriching machine or not.

The invention provides a muskmelon seed germination accelerating method comprising seed selection, seed soaking with a seed soaking agent, microwave nutrient solution seed soaking, refrigerator temperature-varying seed storage, rare earth seed dressing, and finally germination accelerating. The germination accelerating method greatly improves the germination rate of muskmelon seeds, shortens the germination time, and improves the field temperature difference change resistant ability of muskmelon; and planted mature muskmelon is high in sugar content, larger in individual size, high in yield, and long in storage period.

The invention discloses a method for cultivating earthworms. The method comprises a step 1) of adopting dry straw, pig manure and organic fertilizer zymophyte to prepare cultivation basic material; a step 2) of laying an earthworm bed; a step 3) stocking the earthworms; a step 4) of adding the pig manure or a mixture of the pig manure and the organic fertilizer zymophyte to serve as feed; a step 5) of performing daily management; and a step 6) of harvesting adult earthworms. By adding the organic fertilizer zymophyte in the basic material and the feed, the fermentation time can be effectively shortened, the pig manure is heated and deodorized quickly and is quickly fermented into an organic fertilizer, the preparation efficiency of the basic material is improved, the earthworm cultivation time cost is reduced, and the earthworm yield is improved. By adding the organic fertilizer zymophyte into the feed, the feed curing process can be accelerated, feed nutrition ingredients are improved, the growth speed of the earthworms is accelerated, and the nutritional value is improved. The earthworms cultivated according to the method are large in size, nonhazardous and high in yield.

The invention relates to a method for cultivating a new variety of hybridized and backcrossed Argopecten purpuratus. The method is characterized by comprising the following steps of: selecting excellent individuals in a hybridized first filial generation family or group of Argopecten irradians and the Argopecten purpuratus; backcrossing the excellent individuals with the Argopecten purpuratus to establish a backcrossed family or group; selecting hermaphroditic excellent individuals with good representation in the backcrossed family or group; and continuously cultivating at least third generations of self-bred families to obtain the new variety of the hybridized and backcrossed Argopecten purpuratus having the advantages of steady heredity, rapid growth, large individuals and wide temperature tolerance range. According to the method provided by the invention, the new variety can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 30%.

The present invention relates to a bletilla striata rapid propagation and sunshine seedling strengthening method belonging to the technical field of bletilla striata artificial cultivation. The method comprises the following steps: a, seed induced germination culture, to be more specific, seeds taken from bletilla striata capsules are sowed onto a seed induced germination medium for culturing for 50 days to obtain seedlings; b, rooting culture, to be more specific, the seedlings are put on a rooting medium for culturing for 40 days at the temperature of 23-27 DEG C under the light intensity of 2000-2600Lux to obtain rooting seedlings; and c, sunshine seedling strengthening, to be more specific, the rooting seedlings are transferred to a sunshine greenhouses for culturing for 10 days at the temperature of 20-28 DEG C under the light intensity of 3000-4000Lux, and then the light intensity is adjusted to 5000-6000Lux for culturing for 20 to 25 days. The beneficial effects of the bletilla striata rapid propagation and sunshine seedling strengthening method is that: the bletilla striata rapid propagation and sunshine seedling strengthening method can greatly improve seedling and seed quality, the seedling and seed are thick and solid, and even, the planting survival rate is high, and the survival rate reaches over 95%.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

The invention discloses a tissue culture quick propagation method of polygonatum kingianum, which includes the steps of: 1) selecting and sterilizing explants; 2) inoculating the explants onto an induced differentiation culture medium, and culturing the explants for 40-50 days with the light intensity being 1800-2000 Lx, irradiating period being 8-10 h per day and culture temperature being 25-27 DEG C; 3) cutting the tissue, which has adventitious buds and projections, generated in the induced differentiation culture medium into dices, and inoculating the dices onto a proliferation culture medium to perform proliferation cultivation to achieve proliferation of adventitious buds; 4) cutting the buds, of which the cluster buds are not less than 0.5 cm in diameter and not less than 3 cm in height, obtained in the proliferation cultivation into single bodies with leaves cut away, inoculating the single bodies on a rooting culture medium, and culturing the single bodies for 30-35 days with the light intensity being 1800-2000 Lx, irradiating period being 10 h per day at the first 20 days and 12 h per day later, and culture temperature being 25-27 DEG C to obtain rooting seedlings; and 5) performing domestication and seedling hardening. The method is more than 95% in survival rate.

The invention discloses a method for breeding metapenaeus ensis in a rice field. The method comprises the steps of a, rice field selection, wherein a 2-3 mu rice field is selected; b, preparation before young metapenaeus ensis releasing, wherein field cleaning, water cultivation and rice planting are conducted; c, offspring seed releasing, wherein 0.6-0.7 cm long young metapenaeus ensis is released; d, breeding management, wherein feeding, water quality management and disease control are conducted; e, harvesting. The metapenaeus ensis bred with the method has the advantages of being large in size and high in meat quality, the yield is high, and good breeding economic benefits are realized.

The invention relates to an ecological breeding method for mixed breeding of gold saddle rabbitfish and giant freshwater prawns. The ecological breeding method comprises the steps that 1, pond cleaning is performed before water inflowing, pond drying in the sun is performed for 14-16 days, and then water inflowing begins, wherein the salinity of a water body is 3-8; 2, the gold saddle rabbitfish and the giant freshwater prawns are bred in a breeding pond in a mixed mode, wherein the density of the gold saddle rabbitfish is 2500-2600 per mu, and the density of the giant freshwater prawns is 50000-5200p per mu; a seawater floating mixed feed and a giant freshwater prawn feed are fed respectively every day in month before the breeding process; only the seawater floating mixed feed is fed after one month of breeding. By adopting the breeding method, the gold saddle rabbitfish survival rate can be up to 85%, the yield of the gold saddle rabbitfish per mu is up to 192 kg, the yield of the giant freshwater prawn per mu is up to 200 kg, bred giant freshwater prawns are large and few get ill, a breeding effect is remarkable, and the ecological breeding method has a good application prospect.

A method for selectively culturing the fresh-water lobster includes such steps as choosing strong Keao shrimp as female parent, and strong Australia fresh-water lobster as male parent, hybridizing to obtain the first generation lobster, choosing its female lobster as female parent, hybridizing with the male Australia fresh-water lobster to obtain the second generation lobster, and selective culture to obtain high-quality target lobster.