172results about How to "Individual big" patented technology

The invention relates to a method for cultivating a new variety of hybridized and backcrossed Argopecten purpuratus. The method is characterized by comprising the following steps of: selecting excellent individuals in a hybridized first filial generation family or group of Argopecten irradians and the Argopecten purpuratus; backcrossing the excellent individuals with the Argopecten purpuratus to establish a backcrossed family or group; selecting hermaphroditic excellent individuals with good representation in the backcrossed family or group; and continuously cultivating at least third generations of self-bred families to obtain the new variety of the hybridized and backcrossed Argopecten purpuratus having the advantages of steady heredity, rapid growth, large individuals and wide temperature tolerance range. According to the method provided by the invention, the new variety can be cultivated in sea areas in the north, such as Qingdao, Dalian and the like; and, compared with the Argopecten irradians, the yield at the same year is increased by more than 30%.

The invention relates to a breeding method for a new marine backcrossed scallop variety. The breeding method is characterized in that: a best individual is chosen from the first filial generation family or group of Argopecten irradians and Argopecten purpuratus, and is first backcrossed with Argopecten irradians to establish a backcrossed family or group, a best hermaphrodite individual is then chosen from the backcrossed family or the backcrossed group with excellent performance, at least three generations of selved families are then bred in succession, and thereby the new marine backcrossed scallop variety which has the characteristics of stable heredity, fast growth, big individual and wide temperature tolerance range is obtained. The new marine backcrossed scallop variety bred by the breeding method has the characteristics of both Argopecten irradians and Argopecten purpuratus, i.e. fast growth, big individual, wide temperature tolerance range and stable heredity, and can be cultured in northern sea areas such as the sea areas of Tsingtao and Dalian, and moreover, the current yield of the new marine backcrossed scallop variety is increased by more than 30 percent in comparison with the current yield of Argopecten irradians.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

Disclosed is a process for preparing microbiologically active organic fertilizer by utilizing discarded mushroom residue, which comprises the following steps, disintegrating the discarded mushroom residue, mixing with disintegrated stalks of agronomic crops or leftover bits and pieces of food processing factories, moistening with water, making the water content of the material to be about 65-70%, inoculating 2-8% of enzyme production composite microorganism agent, placing them in a fermenting tank, sealing for fermentation 5-15 days at a predetermined temperature, ventilating 15 minutes for every 24 hours during the fermentation process, when the biomass and enzyme vitality of the microorganism reach index point, the microbiologically active organic fertilizer can be obtained. The invention solves the problem of environmental pollution existed in the conventional treatment process for discarded mushroom residue.

The invention provides an earthworm cultivating method which is characterized by comprising preparation of earthworm beds, inoculation of earthworms, management and harvest of adult earthworms. Drainage ditches are arranged among the earthworm beds. Pedestrian paths are arranged on two sides of each earthworm bed. The cross section of each earthworm bed is designed to be a trapezoid. Water conveying pipelines are laid so that a humid growing environment for the earthworms is ensured, the environment of the upper portion of each earthworm bed is more humid and softer than that of the lower portion of each earthworm bed, automatic separation of the adult earthworms and young earthworms is achieved, sundry separating work in a later stage is avoided, and working efficiency is improved. The adult earthworms and the young earthworms grow separately, cultivating space is utilized sufficiently, and population competition is avoided to some extent. The adult earthworms are large in size, bright in color, high in spawning rate and high in high quality protein level, and therefore improvement of yield and quality of the earthworms is facilitated.

The invention discloses a specific antibody of major royal jelly protein MRJP1 and a preparation method thereof and Elisa quantitative detection thereof. First, homology analysis is performed to amino acid sequences of proteins of all members of the Apismellifera major royal jelly protein MRJPs family (MRJP1-MRJP9) to select a specific polypeptide amino acid sequence unlike other MRJPs family members in MRJP1. The related specific polypeptide is synthesized by using a chemical method, and is used as an antigen to immunize New Zealand white rabbits; taking serum, and performing Elisa assay obtaining polyclonal antibody R2 with a relatively high titer, then purifying the antibody by using an affinity column prepared from the synthesized MRJP1 polypeptide. The titer of antibody R2 is detected via Elisa assay by using MRJP1 as the antigen, and the titer of the antibody is greater than 1:20000. The present invention provides a very reliable new rapid detection method for the qualitative and quantitative detection of MRJP1 in royal jelly, and also provides a very reliable technical means for quality control, freshness detection, and identification of genuine products of royal jelly and honey products for bee product quality supervision departments and processing and trading enterprises.

The invention relates to a method for preparing microbial feed additive used for promoting growth of goose liver. All of the microbial strain is screened out from the animal intestinal tract. The method comprises culturing all the strains on the same culture medium through combined culture, constituting powerful compound microbial community by using mixed sync solid fermentation process, and forming effective enzyme producing ability to make the product has amount of physiologic metabolite such as organized enzyme for analysing cellulose and fructose, peptide, antioxidants, free amino acid, and vitamin. The invention can promote feed digestion and absorption of animal, promote synthesize and utilization of protein, enhance immunologic competence of livestock, fowl and aquatic livestock, and improve feed conversion ratio and production capacity of breed animal. The inventive product has good practicability and pertinency.

The invention discloses a freshwater crayfish culturing method, comprising the following steps of: a, selecting a culturing ping; b, preparing before culturing; c, putting into crayfish fingerlings; d, scientifically feeding; e, controlling water quality. According to the above manner, cultured crayfish have the advantages of being large and high in meat quality, and also have high yield and good culturing economic benefit.

The invention discloses a tilapia fry rearing method. The tilapia fry rearing method comprises the following steps: breeding parent tilapia fries which are bigger than nine-sieve, picking parent tilapias which are disease free, injury free, strong and good in sexual maturity to rear, hybridizing the female parent tilapias and with the male parent tilapias according to ratio of stocking, picking up roes, incubating the roes with flowing incubating water in an incubating tank, cultivating the roes by marking in bold, and cultivating fries. Oviposition period is shortened by about seven days, incubating rate is as high as 67.9%, fry survival rate is 89.6%, each female tilapia lays 6700 fries each year averagely, fry production rate is improved by 30%, and the sizes of the fries are uniform. By means of the tilapia fry rearing method, fries can be reared all through a year, a large amount of tilapia fries are reared and the tilapia fries grow fast and are strong in stress resistance. The tilapia fries reared in mass are born at the same time, gonad developments are synchronous, sizes are the same, characters are unified, and heredity purity is high. Moreover, the tilapia fry rearing method has the advantages that incubating period is short, incubating efficiency is high, inheritable characters are good, fertility is strong, and fries can be reared in an overwintering mode.

Cultured Penaeus vannamei Boone has the advantages of high growth speed, strong adaptability and high yield and becomes one of the main cultured varieties in northern areas of China. (1) pond water color control: the ideal color of water for culturing the Penaeus vannamei Boone is yellow green or yellow brown formed by green algae or diatoms; the algae or diatoms are benign biocenosis in the micro-ecological environment of a pond and play a role of purifying water quality; (2) control of pond water pH value, dissolved oxygen and transparency: the pH value suitable for the Penaeus vannamei Boone is 7.8-8.5, but the peak value of 9.2 is generated at the middle and final stages culturing, and therefore, the pH value control is especially focused in the culturing process; the pH value is not suitable to be high, otherwise, the toxicity of ammonia nitrogen can be increased, thereby restraining the growth of the Penaeus vannamei Boone; (3) scientific bait casting: generally, low-cost iced fish cream or small shells are fed, and some formula feeds are also fed; the formula feeds can be manually compounded; the formula feeds contain the following components: 17 percent of fish meal, 40 percent of soya-bean cake, 28 percent of bran, 10 percent of wheat middling, 3 percent of bone meal and 2 percent of additive; and (4) pond patrol: the pond is patrolled in the morning, at night and in the midnight every day to observe the water color change and whether Penaeus vannamei Boone raise above water to breathe or not; the activity condition, the growth condition and the repletion rate of the Penaeus vannamei Boone are observed by adopting two methods of lamplight observing and spoon-net inspection so as to regulate the bait casting amount and decide whether to start an oxygen-enriching machine or not.

The invention provides a method for industrially producing zophobas morio, which includes the following basic steps that: (1) a population is bred by way of purification, crossbreeding and rejuvenation; (2) larvae are bred with a special-made artificial feed under constant temperature and humidity, frass is cleaned up by a sieving machine every 5 to 8 days, most of the larvae are used as commercial larvae, part of the larvae are left for breeding in the next production period, and the rest continue to be cultured into pupae; (3) disinfection is periodically carried out at the pupal stage; (4) adults are fed with concentrate and green material alternately in the morning and the evening; and (5) eggs are collected in egg-laying trays by egg-laying sieves, the egg-laying sieves are taken up after 5 to 7 days, and a thin layer of fine wheat bran is sprinkled on each egg-laying tray to carry out incubation. The method for producing zophobas morio solves the key technical bottlenecks, including low production efficiency, low benefit and heavy labor, and the industrial and mass production of zophobas morio is put into practice.

The invention provides a muskmelon seed germination accelerating method comprising seed selection, seed soaking with a seed soaking agent, microwave nutrient solution seed soaking, refrigerator temperature-varying seed storage, rare earth seed dressing, and finally germination accelerating. The germination accelerating method greatly improves the germination rate of muskmelon seeds, shortens the germination time, and improves the field temperature difference change resistant ability of muskmelon; and planted mature muskmelon is high in sugar content, larger in individual size, high in yield, and long in storage period.

The present invention relates to culture of marine green algae, and is especially the process of fast cultivating marine green algae. The process includes sterilizing and cooling natural sea water and adding trace nutritious elements to form the culture medium; inoculating marine green algae in the density of (5.0-6.0)x10 cells / ml; and lighting culturing in the liquid surface illumination intensity of 3500-4000 Lx and lighting / no-lighting time ratio of 12-14 to 12-10 for 6-8 days to reach algae density of (2.0-2.5)x10 cells / ml. The process has slow change of pH in the algae liquid, high chlorophyll content each algae cell unit and large algae cell. The present invention uses trace elements in culturing marine green algae, and has fast algae cell growing rate and high chlorophyll content each algae cell unit.

The invention discloses a method for cultivating earthworms. The method comprises a step 1) of adopting dry straw, pig manure and organic fertilizer zymophyte to prepare cultivation basic material; a step 2) of laying an earthworm bed; a step 3) stocking the earthworms; a step 4) of adding the pig manure or a mixture of the pig manure and the organic fertilizer zymophyte to serve as feed; a step 5) of performing daily management; and a step 6) of harvesting adult earthworms. By adding the organic fertilizer zymophyte in the basic material and the feed, the fermentation time can be effectively shortened, the pig manure is heated and deodorized quickly and is quickly fermented into an organic fertilizer, the preparation efficiency of the basic material is improved, the earthworm cultivation time cost is reduced, and the earthworm yield is improved. By adding the organic fertilizer zymophyte into the feed, the feed curing process can be accelerated, feed nutrition ingredients are improved, the growth speed of the earthworms is accelerated, and the nutritional value is improved. The earthworms cultivated according to the method are large in size, nonhazardous and high in yield.

Technology for bulk preserving spermatozoa of Chinese sturgeon through freezing at ultralow temperature comprises collecting and detecting fresh spermatozoa, diluting, balancing, adding antifreeze, subpackaging, program cooling, and preserving in liquid nitrogen container of -196deg.C. Each bulk can freeze 100 mL fresh spermatozoa. when the first bulk spermatozoa is in the procedure cooling step, after 5 minute, starting diluting the second bulk spermatozoa, and repeating above steps until all spermatozoa is preserved 8-12 h before its effective time. The diluent is Tris-HCl 20-30 millimole, sucrose 30-60 mol, potassium chloride 2-10 millimole, and distilled water 1000mL, and regulating pH to 8-8.5 with HCl. The antifreeze is methanol. The invention can efficiently carry out bulk preservation of Chinese sturgeon spermatozoa through freezing at ultralow temperature and long term ultralow temperature freezing preservation, and provide enough spermatozoa resource for artificial propagation of Chinese sturgeon.

The invention discloses an ecological circulation culture method and device for algae, prawns and sinonovacula constricta in winter. The device comprises an alga pond, a high-position prawn culture pond, settling ponds, a sinonovacula constricta culture pond and purifying ponds, wherein a thermal-insulation plastic shed covers each pond; the high-position prawn culture pond is provided with a boiler. The method includes: inoculating micro-alga seed sources artificially cultured in the alga pond into the high-position prawn culture pond, the optical prawn culture temperature is guaranteed through boiler heat supply, micro-algae in prawn culture water passing the settling ponds is pumped into the sinonovacula constricta culture pond to serve as baits, and micro-alga seawater filtered by sinonovacula constricta is purified by the purifying ponds and pumped into the high-position prawn culture pond for recycling. The ecological circulation culture method and device has the advantages that the sinonovacula constricta can obtain sufficient micro-alga baits and appropriate growth water temperature in the whole culture period, and the high-position prawn culture pond is allowed to keep a relatively-stable water ecological environment system at the same time.

The invention discloses a method for quickly establishing cross-bred XY holandric pelteobagrus fulvidraco. The method is characterized in that based on mating offspring of pelteobagrus fulvidraco YY physiological female fish and pelteobagrus vachelli XY male fish, cross-bred YY super-male fish is obtained by cultivation through a genetic method so as to mate with common pelteobagrus fulvidraco female fish to produce the XY holandric pelteobagrus fulvidraco. According to the method disclosed by the invention, the breeding period can be obviously shortened; compared with technologies such as Varadaraj and the like, two generations can be shortened; and compared with a method of continuously producing the holandric pelteobagrus fulvidraco by using sex reversal and gynogenesis, one generation can be shortened. Besides, the cross-bred holandric pelteobagrus fulvidraco obtained by the method disclosed by the invention is stable in genetic character, high in growth speed, large, and strong in adaptive capacity to the environment, has cross-breeding advantages and inherits advantages of the holandric fish, so that better breeding and economic benefits can be obtained.

The present invention relates to a bletilla striata rapid propagation and sunshine seedling strengthening method belonging to the technical field of bletilla striata artificial cultivation. The method comprises the following steps: a, seed induced germination culture, to be more specific, seeds taken from bletilla striata capsules are sowed onto a seed induced germination medium for culturing for 50 days to obtain seedlings; b, rooting culture, to be more specific, the seedlings are put on a rooting medium for culturing for 40 days at the temperature of 23-27 DEG C under the light intensity of 2000-2600Lux to obtain rooting seedlings; and c, sunshine seedling strengthening, to be more specific, the rooting seedlings are transferred to a sunshine greenhouses for culturing for 10 days at the temperature of 20-28 DEG C under the light intensity of 3000-4000Lux, and then the light intensity is adjusted to 5000-6000Lux for culturing for 20 to 25 days. The beneficial effects of the bletilla striata rapid propagation and sunshine seedling strengthening method is that: the bletilla striata rapid propagation and sunshine seedling strengthening method can greatly improve seedling and seed quality, the seedling and seed are thick and solid, and even, the planting survival rate is high, and the survival rate reaches over 95%.

The invention relates to a culture method of a new hybrid variety of Argopecten irradias and Argopecten purpuratus. The culture method comprises the following steps of fertilizing Argopecten irradias eggs with Argopecten purpuratus sperms to build multiple hybrid first filial generation lines, selecting excellent hybrid lines according to phenotype of each hybrid line, selecting excellent hermaphroditic individuals from the excellent hybrid lines, maturing the excellent hermaphroditic individuals, and then carrying out self-fertilization to build multiple selfing lines; selecting excellent lines from the selfing lines, selecting excellent hermaphroditic individuals from the excellent lines, carrying out selfing and selecting; and carrying out selfing for more than three generations according to the same method to obtain the new hybrid variety of Argopecten irradias and Argopecten purpuratus. The obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has stable inheritance, rapid growth, large size and wide temperature tolerance range. The culture method also can be implemented by adopting a colony culture method. Since the obtained new hybrid variety of Argopecten irradias and Argopecten purpuratus has the excellent characteristics of Argopecten irradias and Argopecten purpuratus at the same time, the new hybrid variety can be cultivated in Qingdao, Dalian and other northern sea areas and the yield is improved by more than 30% as compared with the yield of bay scallop.

The invention discloses a tissue culture quick propagation method of polygonatum kingianum, which includes the steps of: 1) selecting and sterilizing explants; 2) inoculating the explants onto an induced differentiation culture medium, and culturing the explants for 40-50 days with the light intensity being 1800-2000 Lx, irradiating period being 8-10 h per day and culture temperature being 25-27 DEG C; 3) cutting the tissue, which has adventitious buds and projections, generated in the induced differentiation culture medium into dices, and inoculating the dices onto a proliferation culture medium to perform proliferation cultivation to achieve proliferation of adventitious buds; 4) cutting the buds, of which the cluster buds are not less than 0.5 cm in diameter and not less than 3 cm in height, obtained in the proliferation cultivation into single bodies with leaves cut away, inoculating the single bodies on a rooting culture medium, and culturing the single bodies for 30-35 days with the light intensity being 1800-2000 Lx, irradiating period being 10 h per day at the first 20 days and 12 h per day later, and culture temperature being 25-27 DEG C to obtain rooting seedlings; and 5) performing domestication and seedling hardening. The method is more than 95% in survival rate.

The invention discloses a pear tree fertilizer slow-release additive containing brassinolide. The pear tree fertilizer slow-release additive containing brassinolide comprises, by weight, 20-25 parts of rice straw, 4-6 parts of coal gangue powder, 0.2-0.4 part of brassinolide, 1-2 parts of guanylthiourea, 1-4 parts of glucose, 1-2 parts of polyethylene glycol, 1-2 parts of brown sugar, 1-2 parts of ardealite, 6-8 parts of weathered coal, 1-3 parts of polyacrylamide, 0.2-0.3 part of potassium nitrophenolate, 0.6-0.8 part of polypropylene glycol, 1-3 parts of agricultural rare earth nitrate, 1-2 parts of sodium dodecyl benzene sulfonate, 4-5 parts of alfalfa, 1-2 parts of flos chrysanthemi indici, 1-2 parts of plumeria rubra, 1-3 parts of stellera chamaejasme, 3-5 parts of corn gluten meal, 3-5 parts of cow dung and 0.1-0.3 part of HM decomposition maturing agent.

The invention discloses a method for cultivating American cockroaches. The method comprises the following steps of a, taking one-age nymphs of the American cockroaches to conduct dark cultivation, wherein the cultivation temperature is 29-31 DEG C and the relative humidity is 80-85%, and obtaining 6-8-age nymphs; b, continuously conducting dark cultivation for 80-100 days, wherein the cultivation temperature is 28-30 DEG C, and the relative humidity is 65-75%. The method for cultivating the American cockroaches is high in the survival rate, the prepared American cockroaches are high in medicinal component content, and the method is short in cultivation period and low in cost.

A breeding method for a new strain of Jianyang big-eared sheep relates to the technical field of animal breeding, in particular to a breeding method for a new strain of Jianyang big-eared sheep. The purpose of the invention is to provide a goat breed with fast growth and development, strong adaptability, high meat production rate and slaughter rate, and a reproduction rate of more than 210%. After long-term selection and breeding, the present invention has the conditions of an excellent population in terms of quality, quantity and genetic performance, and has formed a population with high reproduction rate, large individual, fast growth and development, good meat production performance, rough feeding resistance, and strong disease resistance. , good leather, tender meat, no smell and other characteristics of the variety, and basically formed an excellent variety with relatively stable genetic performance and a certain population size, which is easy to promote.

The invention provides a method of identifying day age of cephalopods through beaks and includes a method of grinding a beak longitudinal section and counting growth striation. The method mainly includes the steps of: extraction, cleaning, soaking, cutting, arrangement, resin embedding, cutting, immobilization on a glass slide, grinding and polishing, picture forming, and growth striation counting. In the method, the beak longitudinal section is ground respectively from the left and right sides to a core surface towards a central line. A prepared section is photographed by CCD to ensure that the growth striation can be read at any time in future. The growth striation is counted from the rear end to the front end of the beak in a direction being vertical to the growth striation all the time, thereby ensuring counting accuracy. The method solves the problems that the beak is difficult to grind and is liable to break during grinding, and the growth striation is difficult to observe and count.

The invention discloses a method for breeding metapenaeus ensis in a rice field. The method comprises the steps of a, rice field selection, wherein a 2-3 mu rice field is selected; b, preparation before young metapenaeus ensis releasing, wherein field cleaning, water cultivation and rice planting are conducted; c, offspring seed releasing, wherein 0.6-0.7 cm long young metapenaeus ensis is released; d, breeding management, wherein feeding, water quality management and disease control are conducted; e, harvesting. The metapenaeus ensis bred with the method has the advantages of being large in size and high in meat quality, the yield is high, and good breeding economic benefits are realized.

The invention relates to an ecological breeding method for mixed breeding of gold saddle rabbitfish and giant freshwater prawns. The ecological breeding method comprises the steps that 1, pond cleaning is performed before water inflowing, pond drying in the sun is performed for 14-16 days, and then water inflowing begins, wherein the salinity of a water body is 3-8; 2, the gold saddle rabbitfish and the giant freshwater prawns are bred in a breeding pond in a mixed mode, wherein the density of the gold saddle rabbitfish is 2500-2600 per mu, and the density of the giant freshwater prawns is 50000-5200p per mu; a seawater floating mixed feed and a giant freshwater prawn feed are fed respectively every day in month before the breeding process; only the seawater floating mixed feed is fed after one month of breeding. By adopting the breeding method, the gold saddle rabbitfish survival rate can be up to 85%, the yield of the gold saddle rabbitfish per mu is up to 192 kg, the yield of the giant freshwater prawn per mu is up to 200 kg, bred giant freshwater prawns are large and few get ill, a breeding effect is remarkable, and the ecological breeding method has a good application prospect.

Disclosed is a diced breeding technique for Chinese narcissus which comprises, using one or two year-old narcissus bulb as raw material, cryogenic processing 4-8 weeks, cutting the bulb longitudinally into uniform small blocks in a radiation shape by using plateau as the center at normal temperature, with each block keeping the plateau and all the flakes, loading each cut small blocks of breeding material into poly bag containing washed and filtered dried sand, sealing the pocket opening, placing in dark places, shifting for culture in the open. The ball forming rate for cutting breeding and transplanting survival rate can be as high as 100%.

The invention provides a daphnia magna domestication method and application thereof. The method comprises the following steps: feeding daphnia magna into a domestication pool, cultivating the daphnia magna for a certain time period with specific domestication food, repeatedly domesticating and screening dominant species, and performing stabilization and rejuvenation to finally domesticate the daphnia magna into natural enemies of cyanophyta. The invention further discloses the domestication food for domesticating the daphnia magna and the application of the daphnia magna domestication method to the field of ecological water body remediation. The daphnia magna domesticated by the method has the characteristics of large individuals, high cyanophycean toxin conversion capability and high outdoor stability; when the domesticated daphnia magna is used for ecological remediation, underwater lighting conditions can be improved, the clarity of a water body can be rapidly improved, and quick response, environment friendliness, low carbon and high efficiency are achieved.

A method for selectively culturing the fresh-water lobster includes such steps as choosing strong Keao shrimp as female parent, and strong Australia fresh-water lobster as male parent, hybridizing to obtain the first generation lobster, choosing its female lobster as female parent, hybridizing with the male Australia fresh-water lobster to obtain the second generation lobster, and selective culture to obtain high-quality target lobster.