Method of identifying day age of cephalopods through beak

Abstract

The invention provides a method of identifying day age of cephalopods through beaks and includes a method of grinding a beak longitudinal section and counting growth striation. The method mainly includes the steps of: extraction, cleaning, soaking, cutting, arrangement, resin embedding, cutting, immobilization on a glass slide, grinding and polishing, picture forming, and growth striation counting. In the method, the beak longitudinal section is ground respectively from the left and right sides to a core surface towards a central line. A prepared section is photographed by CCD to ensure that the growth striation can be read at any time in future. The growth striation is counted from the rear end to the front end of the beak in a direction being vertical to the growth striation all the time, thereby ensuring counting accuracy. The method solves the problems that the beak is difficult to grind and is liable to break during grinding, and the growth striation is difficult to observe and count.

Description

technical field [0001] The invention belongs to the field of fisheries, and mainly relates to a method for identifying the age of cephalopods by using horny jaws. Background technique [0002] Cephalopods belong to molluscs, are generally annuals, and have the characteristics of dying after laying eggs. They are important marine economic species and are widely distributed in the world's oceans and Antarctic sea areas. [0003] Age determination is an important research topic in cephalopod fishery biology and ecology. The age identification methods of cephalopods can be divided into indirect and direct methods. Due to the characteristics of cephalopods such as fast growth, long spawning period, and complex population structure, the indirect estimation of age by carcass length or body weight is not accurate enough. Among all the direct methods, the use of growth lines in hard tissues is considered to be the most effective method for age identification of cephalopods. [000...

AI Technical Summary

Problems solved by technology

In the prior art, scissors are usually used to directly cut the beak of the horny jaw with scissors in the production of slices of the horny jaw of cephalopods. This operation is likely to cause the fracture of the beak section and affect the interpretation of growth lines; secondly, the grinding of the beak of the horny jaw is direct grinding , on the one hand, it will cause the cuticle to break during the grinding process, and on the other hand, it will cause the offset of the grinding plane, which will increase the damage to the cutin during the grinding process, and the success rate of slicing is low.

In addition, in the prior art, counting the growth lines of the horny palate is directly observed under a microscope, and it is extremely inconvenient to count the growth lines; The growth lines at the junction of the dorsal side and the re-measurement (the dotted line in the figure), and this counting method will mistake some sub-daily lines for sun lines, resulting in a large error

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