38 results about "Metapenaeus ensis" patented technology

The invention discloses a hydrophilic interaction chromatography-tandem mass spectrometry detection method of phospholipids in Metapenaeus ensis. The method includes the steps of firstly, performing corresponding preprocessing on ground Metapenaeus ensis to obtain crude lipid extract; secondly, performing liquid phase separation on the crude lipid extract, wherein a chromatographic column is a YMC Triart diol HILIC column, a gradient elution method is used, a gradient system comprises a flowing phase A and a flowing phase B, the flowing phase A is an acetonitrile solution containing 53mmol / L formic acid, and the flowing phase B is an aqueous solution containing 60mM ammonium formate and 53mM formic acid; thirdly, performing mass spectrometric detection analysis on the elution liquid obtained in the second step. By the method, the total content of PE, PS and PC in the Metapenaeus ensis can be detected accurately, and mass spectrometry identification of various molecular species in the PC, PE and PS phospholipids can be achieved.

The invention discloses sheep appetite promoting feed taking apple pomace as a raw material. The feed is prepared from the following raw materials in parts by weight: 500-550 parts of apple pomace, 20-30 parts of sodium lactate, 54-56 parts of moringa leaf, 25-27 parts of flower of kudzuvine, 5-7 parts of blueberry jam, 84-92 parts of peanut meal, 32-36 parts of locust powder, 3-5 parts of garlic paste, 15-17 parts of metapenaeus ensis, 3-4 parts of lentinus edodes powder, 4-5 parts of spinach powder, 72-76 parts of barley flour, 3-5 parts of aromatic vinegar, 55-58 parts of sweet potato residue, 34-36 parts of buckwheat bran, 0.4-0.5 part of candida utilis, 33-35 parts of bamboo leaf powder, 24-26 parts of pawpaw residue, 0.4-0.5 part of table salt and 2-3 parts of bone meal. The feed disclosed by the invention is sour and sweet and fragrant, has good taste, promotes the digestive function of sheep, improves the sheep appetite, promotes the sheep absorption of nutrient substances and enhances the stress resistance and disease resistance of sheep; and moreover, the feed has abundant nutrition and ensures that sheep can quickly grow and has strong immunity, robust body and good mutton quality.

The invention discloses a method for breeding metapenaeus ensis in a rice field. The method comprises the steps of a, rice field selection, wherein a 2-3 mu rice field is selected; b, preparation before young metapenaeus ensis releasing, wherein field cleaning, water cultivation and rice planting are conducted; c, offspring seed releasing, wherein 0.6-0.7 cm long young metapenaeus ensis is released; d, breeding management, wherein feeding, water quality management and disease control are conducted; e, harvesting. The metapenaeus ensis bred with the method has the advantages of being large in size and high in meat quality, the yield is high, and good breeding economic benefits are realized.

The present invention discloses a metapenaeus ensis cultivation method. The metapenaeus ensis cultivation method comprises: step 1, selecting a cultivation pond; after exposing the cultivation pond under the sun, wiping an inner wall of the cultivation pond by using kaempferiae slices and then exposing the cultivation pond under the sun again; coating the inner wall of the cultivation pond by medicine liquid and exposing the cultivation pond under the sun, and repeating the wiping and exposing for 3-4 times; blending quick lime with water and spraying on the inner wall of the cultivation pond, and then exposing the cultivation pond under the sun; step 2, controlling a water temperature, a pH value and salinity of pond water; then adding young shrimps into the cultivation pond; feeding young shrimp feed every day from the first day to the 20th day; adding adult shrimp feed every day after 20 days; and step 3, oxygenating the pond water of the cultivation pond every 2-3 days, replacing the water of the cultivation pond every 4-5 days, and adding moroxydine into the cultivation pond every 30-40 days. With the adoption of the metapenaeus ensis cultivation method, the immunity and resistance of metapenaeus ensis can be enhanced, the growth of the metapenaeus ensis is accelerated and the survival rate and meat quality of the metapenaeus ensis are improved.

The invention relates to a metapenaeus ensis ball and a preparing process thereof. The spicy shrimp ball is prepared by mixing fresh metapenaeus ensis with shrimp meat, chicken breast meat, ginger, green onion, eggs, starch, salt, aginomoto and ground pepper into pulp, cooling down the pulp, preparing small balls by using a forming machine through the pulp, putting the small balls into salad oil of 60 DEG C to fry, naturally cooling down, sterilizing and putting the small balls into sterilized bags according to rated weights. The original ingredients of the metapenaeus ensis are kept unchanged, no preservatives are added, and the metapenaeus ensis ball is easy to preserve and eat, has the taste of freshness, smoothness and tenderness, and is applicable to preparing in batch.

The invention discloses a water-saving metapenaeus ensis culture pond, comprising a culture pond body and a cover body. The water-saving metapenaeus ensis culture pond is characterized in that two opposite edges of the top of the culture pond body are provided with slide rails, the bottom of the cover body is provided with rollers, the rollers are slidably connected with the slide rails, the side face of the culture pond body is provided with a vertical rod, a step motor is arranged on the vertical rod and is connected with a screw rod, a movable block is arranged on the screw rod and is connected with the cover body, and the step motor is connected with a control module connected with a setting module. The cover body in the water-saving metapenaeus ensis culture pond can be opened and closed in a timed way, so too fast evaporation of water is prevented, and the water-saving effect is obvious.

The invention discloses a method for breeding metapenaeus ensis in a rice field. The method comprises the first step of field clearing, the second step of fry putting, the third step of feed putting, the fourth step of managing and the fifth step of fishing. According to the method, a metapenaeus ensis and rice field ecological breeding mode is adopted, metapenaeus ensis breeding and rice planting can be organically combined, mutualism is achieved between the metapenaeus ensis and rice, the productivity of the field is brought into full play, and the economic benefits of field management is improved; the metapenaeus ensis bred through the method has the advantages that the sizes are large, the meat quality is high, meat is delicious and nutrition is abundant, the yield is increased, and good economic benefits of breeding are achieved; compared with a traditional breeding method, the method has the advantages that the metapenaeus ensis grows quickly, specifications are uniform, and the meat quality and the edible value of the metapenaeus ensis are improved.

The invention provides a metapenaeus ensis farming method. The metapenaeus ensis farming method comprises the following steps of pond selection, pond clearing and sterilization, shrimp larvae putting, feed putting and daily management. The metapenaeus ensis farmed scientifically by adopting the metapenaeus ensis farming method is delicious in flesh, large in size and high in yield, and the maximum economic profit is brought to farmers. The immunity and resistance of the metapenaeus ensis can be improved, the metapenaeus ensis growth speed is improved, and the survival rate and flesh quality are improved.

The invention discloses a metapenaeus ensis culture pond which comprises a pond body; a string bag with an opening in the top is adhered to the inner wall of the pond body; a pulling wire is connectedto the top of the string bag; a reel which spans the pond body is hinged to the top of the pond body through a bracket; the bracket is provided with a rolling curtain motor; a rotary shaft of the rolling curtain motor is connected with one end of the reel; the reel is wound with the upper part of the pulling wire; a drainage hole is formed in the middle part of the bottom of the pond body; a drainage pipe is connected to the drainage hole and is provided with a drainage pump; scrubbing brushes used for brushing the bottom of the pond body are symmetrically arranged on the two side walls of the pond body; a cylinder which is used for driving the scrubbing brushes to perform back and forth motion is installed at the bottom of the pond body; the rolling curtain motor, the drainage pump and an electromagnetic reversing valve which is communicated with the cylinder are all electrically connected with a controller; and the controller is installed on the bracket. The metapenaeus ensis culture pond provided by the invention effectively saves water resources and lowers the culture cost while ensuring full cleaning of excrements of metapenaeus ensis.

The invention discloses a culture method for DHA-rich Metapenaeus ensis. The Metapenaeus ensis is fed with a feedstuff prepared from the following raw materials in parts by weight: 20-30 parts of peanut powder, 10-25 parts of bean pulp, 2-5 parts of earthworm powder, 0-10 parts of Schizochytrium limacinum powder, 10-25 parts of Schizochytrium limacinum waste residue, 1-5 parts of Bacillus natto, 0.2-0.5 part of yeast extract, 10-25 parts of maize germ oil and 1-5 parts of herba houttuyniae extracting solution. The culture method disclosed by the invention is low in cost, safe and reliable, and causes no pollution; the Metapenaeus ensis is fed with the feedstuff using the Schizochytrium limacinum and the waste residue thereof as the raw materials, thereby increasing the DHA and EPA contents in the prawn meat, enhancing the immunity of the Metapenaeus ensis, and promoting the growth and development of the Metapenaeus ensis. The culture method is applicable to large-scale culture of DHA-rich safe and functional Metapenaeus ensis products.

A metapenaeus ensis cultivation management system comprises a water body physical index detection signal emission device, a water body chemical index synchronous detection device, a central processing platform and a user terminal. According to the system, actual measurement results of eight indexes, including temperature, dissolved oxygen, pH, ammonia nitrogen, nitrite, permanganate, phosphorus content and nitrogen content, of to-be-detected culture pond water quality under control can be transmitted to the central processing platform; the received eight key technical indexes are compared with water quality technical parameters preset in metapenaeus ensis cultivation technical specifications through the central processing platform; and when the corresponding indexes deviate from the specified technical parameters, control information can be sent to the corresponding user terminal according to a preset handling scheme, and the user performs handling in time according to the handling scheme received in a mobile phone.

The present invention discloses a self-heating hot pot metapenaeus ensis meat package and a preparation method thereof. After pretreatment of metapenaeus ensis, the pre-treated metapenaeus ensis is subjected to ultrasonic soaking with preservation liquid, then the soaked metapenaeus ensis is pre-heated in a microwave oven, the pre-heated metapenaeus ensis is taken out to be soaked with ice water for 20-30 s, then the soaked metapenaeus ensis is put into a steaming basket to be steamed for 2 min, the steamed metapenaeus ensis is taken out, the taken out metapenaeus ensis is placed in a chitosansolution to be cooled until a center temperature of the metapenaeus ensis meat is 20-25 DEG C, and finally vacuum packaging is conducted under sterile conditions. After the specially prepared preservation liquid is subjected to high-speed stirring and vacuum homogenization, the preservation liquid can directly act inside the metapenaeus ensis meat, thus significantly increases water holding capacity of the metapenaeus ensis meat, can also catalyze intramolecular and intermolecular covalent crosslinking of metapenaeus ensis meat protein polypeptides, thus improves structures and functions of the metapenaeus ensis meat proteins, improves freezing resistance of the metapenaeus ensis meat, reduces protein denaturation, at the same time can also provide delicious mouthfeel for the heated metapenaeus ensis meat, and preserves flavor substances. The used technology also has characteristics of low energy consumption and short processing cycles, and truly improves economic value of the metapenaeus ensis in pre-conditioned food.

The present invention relates to the use of alpha lipoic acid as feed additive for aquatic animals including fish and shrimp, especially for cold water fish as for example salmon, bream, bass and forwarm water fish as for example carp, tilapia, catfish. More particular, this invention relates to the use of alpha lipoic acid for the improvement of the feed conversion ratio and / or daily weight gainin fish, for reducing mortality by regulating the micro flora of the gut and / or by protecting the animal against infections caused by pathogenic viruses.

The invention discloses a formula of Metapenaeus ensis disease-resistant feed. The Metapenaeus ensis disease-resistant feed is prepared from 10-20 parts by weight of corn powder, 3-4 parts by weight of soybean polypeptide, 1-2 parts by weight of calcium hydrogen phosphate, 0.5-1.4 parts by weight of stone flour, 0.01-0.1 parts by weight of lysine with a concentration of 98%, 4-6 parts by weight of cottonseed meal, 12-14 parts by weight of purslane, 10-15 parts by weight of wheat bran, 2-3 parts by weight of lotus leaves, 13-27 parts by weight of bean dregs, 1-2 parts by weight of bitter orange, 1-2 parts by weight of ligusticum wallichii, 1-2 parts by weight of fish oil, 10-15 parts by weight of peanut meal, 1-3 parts by weight of zinc, 1-3 parts by weight of iodine, 1-3 parts by weight of VC, 3-8 parts by weight of brewer yeast, 12-15 parts by weight of dried orange peel, 1-3 parts by weight of seaweed meal, 0.5-1 part by weight of garlic powder, 1-2 parts by weight of roses, 0.1-0.4 parts by weight of table salt, 10-15 parts by weight of radix pulsatillae, 1-5 parts by weight of threonine, 1-2 parts by weight of isatis root, 1-2 parts by weight of grosvenor momordica fruits and 1-2 parts by weight of bighead atractylodes rhizome. Compared with the existing feed, the Metapenaeus ensis disease-resistant feed utilizes combination of coarse grains and extract, utilizes traditional Chinese medicines with effects of clearing fire, has a scientific formula, is suitable for Metapenaeus ensis growth and development, has virus resistance, reduces Metapenaeus ensis mortality by 25% and improves a yield by 62%.

The invention relates to a special feed for metapenaeus ensis and a processing method, and belongs to the technical field of processing of aquaculture feeds. Raw materials of the special feed comprise the following components in parts by weight: 20-30 parts of fish meal, 8-15 parts of corn starch, 10-15 parts of wheat flour, 5-10 parts of bone meal, 3-6 parts of blood powder, 5-10 parts of dried marine algae powder, 3-8 parts of fish oil, 0.3-0.7 part of lysine, 0.3-0.5 part of methionine, 0.2-0.7 part of protease, 0.5-2.5 parts of complex vitamins and 0.8-3.0 parts of composite mineral substances. The processing method comprises the following steps of (1) weighing the raw materials; (2) mixing the weighed raw materials; (3) pulverizing the mixed materials; (4) quenching and tempering the pulverized materials, and ageing the quenched and tempered materials; (6) performing granulation; (7) drying granules; (8) cooling the dried granules; and (9) crumbling the cooled granules with a roller type granule crumbler. According to the formula of the special feed disclosed by the invention, in accordance with the growth characteristics of the metapenaeus ensis, an appropriate amount of the bone meal and an appropriate amount of the blood powder are added to the formula, so that calcium and heme desired for the metapenaeus ensis are increased, and the nutrition in the feed can meet the growth need of the metapenaeus ensis; the lysine and the methionine are added, so that the utilization rate of plant peptide hydrolysates is increased, the nutritional absorption is promoted, the digestibility of the raw materials is improved, besides, the taste is strengthened, the lower feed coefficient of the metapenaeus ensis is guaranteed, and the specific growth rate is high.

The invention discloses shrimp feed. The shrimp feed consists of the following formula components in parts by mass: 8 to 12 parts of laver powder, 2 to 4 parts of trollflower, 10 to 20 parts of plant feed, 3 to 5 parts of gallnut, 2 to 4 parts of soyabean lecithin, 5 to 15 parts of alga meal, 2 to 4 parts of szechuan melandium root, 10 to 20 parts of sorghum flour and 2 to 4 parts of soya bean oil. The formula of the shrimp feed can reduce diseases of shrimps and prawns; the feed is health; the source is wide, and the cost is low.

Metapenaeus ensis feed is prepared from the following components in parts by weight: 20 to 30 parts of corn, 10 to 15 parts of wheat bran, 15 to 20 parts of bean cake, 8 to 15 parts of bone meal, 8 to15 parts of fish meal, 6 to 10 parts of peanut powder, 0.5 to 2 parts of table salt, 5 to 8 parts of carrots, 3 to 6 parts of sweet potatoes, 3 to 6 parts of folium isatidis, 4 to 7 parts of fructuscrataegi, 5 to 8 parts of rhizoma dioscoreae, 3 to 6 parts of liquorice root, 3 to 5 parts of radix astragali seu hedysari, 3 to 5 parts of radix gentianae, 3 to 6 parts of honeysuckle flower, 10 to 16 parts of apples, 5 to 10 parts of fructus lycii, 2 to 5 parts of rhizoma phragmitis, 0.5 to 1 part of garlic bulb and 2 to 4 parts of radix salviae miltiorrhizae. The metapenaeus ensis feed disclosed by the invention not only is balanced in nutrition, but also is capable of effectively preventing virus diseases of metapenaeus ensis, greatly enhancing immunity of the metapenaeus ensis and quickening growth, has no residual damage and is natural and environment-friendly green food.

The invention discloses a method for cultivating metapenaeus ensis. The method comprises the following steps of (1) pond conditions; (2) stocking of offspring seeds; (3) feeding management; (4) fishing. By means of the method, the metapenaeus ensis is fat with thin shells, and is tender and tasty, and production cost is low.

The invention belongs to the field of gene engineering, and particularly relates to a prawn Y-organ 7,8-dehydrogenase gene and application thereof. The invention provides a gene for coding 7,8-dehydrogenase, which is obtained from a constructed Metapenaeus ensis Y-organ cDNA (complementary deoxyribonucleic acid) library; and the nucleotide sequence of the gene is disclosed as SEQ ID NO.1. The application method comprises the following steps: cloning the 7,8-dehydrogenase coding gene, and connecting with an expression plasmid to construct a recombinant expression plasmid; after linearizing and purifying the recombinant expression plasmid, converting a receptor bacterium, and screening to obtain a gene engineering bacterium; and carrying out induction expression, purification and identification to obtain the 7,8-dehydrogenase of which the amino acid sequence is disclosed as SEQ ID NO.2. The 7,8-dehydrogenase can be used for converting cholesterol into 7-dehydrocholesterol by a one-step enzyme process, thereby enhancing the conversion efficiency, shortening the process route for producing vitamin D3, lowering the production cost and reducing the environmental pollution.

The invention provides small-sized shrimp overwintering feed and a preparation method thereof. The feed is prepared from the following raw materials: defatted fish meal, greenlip mussel meal, high gluten flour, fermented blood meal, seaweed meal, sleeve-fish liver powder, peeled soybean meal, cotton seed meal, haematococcus pluvialis, peanut meal, lycopene, shrimp shell meal, a feeding attractant and plant oil. The feed disclosed by the invention has the beneficial effects that the additives of the feed disclosed by the invention have scientific compatibility, malnutrition in ordinary small-sized shrimp feed is avoided, growth of the small-sized shrimps in winter is promoted, the feed has the effects of resisting bacteria, sterilizing and inhibiting viruses and can obviously improve the body immunity, cold resistance and survival rate of the shrimps, and traditional Chinese medicine components in the feed have the advantages of being low in cost, convenient to process, free of drug resistance and drug residue, small in side effects and obvious in effect.

The present invention discloses a feather hydrolysis powder feed used for metapenaeus ensis and capable of improving disease resistance by combination of antimicrobial peptide and cordyceps sinensis polysaccharide, and a preparation method thereof. The feed combines the antimicrobial peptide and the cordyceps sinensis polysaccharide, can improve the disease resistance of the metapenaeus ensis, enhance the immune function of juvenile metapenaeus ensis, and conduct comprehensive conditioning on the physical function of the metapenaeus ensis. The feed can improve the disease resistance and the stress resistance of the metapenaeus ensis, promote the growth and development of the metapenaeus ensis, and increase aquaculture production and survival rate. The feed is natural, healthy, palatable and easy to prepare.

The present invention relates to unfermented piper sarmentosum flavored seafood sauce and a preparation method thereof. The piper sarmentosum flavored seafood sauce consists of the following components in parts by mass: 125-500 parts of metapenaeus ensis, 125-500 parts of lingulae, 63-250 parts of piper sarmentosum leaves, 250-1,000 parts of Pixian thick broad-bean sauce and 250-1,000 parts of salad oil. The preparation method of the unfermented piper sarmentosum flavored seafood sauce is as follows: blend oil is heated and the raw materials are stir-fried to be fragrant and even to obtain the piper sarmentosum flavored seafood sauce. The piper sarmentosum leaves are added into the metapenaeus ensis and lingulae prepared sauce, on a basis of the seafood sauce, delicate fragrance of a special plant of the piper sarmentosum leaves is increased, the preparation method reduces stink odor of the seafood sauce, also increases new flavored characteristics and improves health-care effects of the seafood sauce, and the obtained seafood sauce has advantages of being mellow in flavor, delicious, fragrant and palatable, long in aftertaste, etc. The provided piper sarmentosum flavored seafood sauce is prepared by the stir-frying method, short in operation time and simple in technology. Besides, the preparation method focuses on controls of fire and matching and use of the raw materials on a technology, and provides the new seafood sauce.

The invention provides a metapenaeus ensis fast-growing strain group breeding method which includes the steps: (1) parent acquisition; (2) parent transportation; (3) marking and pathogen detection; (4) parent spawning; (5) first-generation offspring pathogen detection; (6) standardization; (7) screening; (8) nutrient enrichment; (9) mating and spawning. Compared with the prior art, metapenaeus ensis offspring groups bred by the metapenaeus ensis fast-growing strain group breeding method have the advantages that pathogens are not carried, growth speed is high, and metapenaeus ensis individualsare uniform in size specification, good in condition and high in genetic diversity.

The invention discloses a hydrophilic interaction chromatography-tandem mass spectrometry detection method of phospholipids in Metapenaeus ensis. The method includes the steps of firstly, performing corresponding preprocessing on ground Metapenaeus ensis to obtain crude lipid extract; secondly, performing liquid phase separation on the crude lipid extract, wherein a chromatographic column is a YMC Triart diol HILIC column, a gradient elution method is used, a gradient system comprises a flowing phase A and a flowing phase B, the flowing phase A is an acetonitrile solution containing 53mmol / L formic acid, and the flowing phase B is an aqueous solution containing 60mM ammonium formate and 53mM formic acid; thirdly, performing mass spectrometric detection analysis on the elution liquid obtained in the second step. By the method, the total content of PE, PS and PC in the Metapenaeus ensis can be detected accurately, and mass spectrometry identification of various molecular species in the PC, PE and PS phospholipids can be achieved.

The invention discloses application of metapenaeus ensis in preparation of angiotensin converting enzyme (ACE) inhibitors. Preferably, metapenaeus ensis is the product obtained after meat paste of metapenaeus ensis is hydrolyzed by simulated gastric fluid, simulated gastrointestinal fluid and protease. The invention presents a new approach to development and utilization of metapenaeus ensis and the metapenaeus ensis has certain application prospect.

The invention discloses a method for detecting specific IgE (immunoglobulin E) of metapenaeus ensis and belongs to the cross field of materials and biomedicine. FHMNs (fluorescent hollow mesoporous silica nanoparticles) are adopted as a vector of an anti-IgE antibody, Fe3O4@SiO2 prepared through coating of Fe3O4 with SiO2 is used as an allergen vector, the two materials capture targeted sIgE (specific IgE) of the metapenaeus ensis simultaneously, the targeted sIgE is quickly enriched and separated by use of magnesium of Fe3O4@SiO2, the signal of low-concentration targeted sIgE is amplified by use of fluorescent molecular signals in the FHMNs, the fluorescence intensity is associated with concentration of the IgE, and the sIgE of the metapenaeus ensis in human blood is detected quickly and sensitively. With the adoption of the method, the IgE detection limit is effectively reduced and the detection time is shortened.

The invention discloses strange-taste vegetable shrimp dried tofu, which is prepared from the following raw materials in parts by weight: 80 to 100 parts of soybean, 8 to 10 parts of red bean paste, 5 to 8 parts of fresh metapenaeus ensis, 1 to 2 parts of fresh lily bulb, 1 to 2 parts of celery, 1 to 2 parts of cayenne pepper, 0.3 to 0.4 part of table salt, 0.4 to 0.5 part of corn starch, 0.1 to 0.2 part of chicken powder, 0.8 to 0.9 part of egg white, 0.4 to 0.5 part of cooking wine, 1 to 2 parts of soup stock, 1 to 2 parts of corn starch water, 0.5 to 0.7 part of white soy sauce, 0.05 to 0.07 part of chopped ginger, 0.05 to 0.07 part of chopped garlic, 0.01 to 0.02 part of seed powder of Chinese prickly ash, 0.02 to 0.03 part of white sugar, 0.1 to 0.2 part of aromatic vinegar, 0.05 to 0.07 part of fistular onion stalk, 0.1 to 0.2 part of sesame paste and 0.03 to 0.05 part of monosidum glutamate. The dried tofu has a unique taste, a seafood flavor, high nutritional value and smaller calories compared with traditional pure meat food.