Method for detecting specific IgE (immunoglobulin E) of metapenaeus ensis

Abstract

The invention discloses a method for detecting specific IgE (immunoglobulin E) of metapenaeus ensis and belongs to the cross field of materials and biomedicine. FHMNs (fluorescent hollow mesoporous silica nanoparticles) are adopted as a vector of an anti-IgE antibody, Fe3O4@SiO2 prepared through coating of Fe3O4 with SiO2 is used as an allergen vector, the two materials capture targeted sIgE (specific IgE) of the metapenaeus ensis simultaneously, the targeted sIgE is quickly enriched and separated by use of magnesium of Fe3O4@SiO2, the signal of low-concentration targeted sIgE is amplified by use of fluorescent molecular signals in the FHMNs, the fluorescence intensity is associated with concentration of the IgE, and the sIgE of the metapenaeus ensis in human blood is detected quickly and sensitively. With the adoption of the method, the IgE detection limit is effectively reduced and the detection time is shortened.

Description

technical field [0001] The invention relates to a method for detecting the specific immunoglobulin E of Penaeus lanceolata, which belongs to the intersection field of materials and biomedicine. Background technique [0002] Shrimp and crab are the most common crustaceans in my country's aquatic product market, and they are high-protein and high-nutrition foods. But it is also one of the eight types of highly allergenic foods, with an incidence rate of 15.6%-35%, leading to the occurrence of allergic asthma, anaphylactic shock and other diseases. [0003] At present, my country is in its infancy in the field of allergy-related research, and there are no relevant diagnostic reagents for shrimp and crab food allergy. Clinical detection reagents mainly rely on imports, such as the Immuno CAP system automatic detection system certified by the US FDA, its recognized detection limit is 0.24ng / mL, and the detection cost is relatively expensive, most allergic patients cannot afford ...

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Problems solved by technology

But it is also one of the eight types of highly allergenic foods, with an incidence rate of 15.6%-35%, leading to the occurrence of allergic asthma, anaphylactic shock and other diseases

[0003] At present, my country is in its infancy in the field of allergy-related research, and there are no relevant diagnostic reagents for shrimp and crab food allergies

Clinical detection reagents mainly rely on imports, such as the Immuno CAP system automatic detection system certified by the US FDA, its recognized detection limit is 0.24ng / mL, and the detection cost is relatively expensive, most allergic patients cannot afford it; The wide range of ELISA detection kits has a high detection limit and cannot accurately identify whether they are allergic patients

Moreover, the allergens used in allergy testing are crude extraction products of biological materials. The crude extraction products contain allergens, non-specific allergens, and suspected allergens that have not yet been characterized, which interfere with the results of allergy testing, and their sensitization efficiency cannot be standardized.

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