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Hydrophilic interaction chromatography-tandem mass spectrometry detection method of phospholipids in Metapenaeus ensis

A new prawn and hydrophilic chromatography technology is applied in the field of food detection, which can solve the problems of direct coupling of mass spectrometry equipment and inability to eluent, and achieve the effects of stable and reliable results, simple training and strong practicability.

Active Publication Date: 2016-11-23
ZHEJIANG GONGSHANG UNIVERSITY
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Problems solved by technology

[0005] The current conventional method for lipid separation is normal phase chromatography, which requires special chromatographic equipment and mobile phases (n-hexane, isopropanol, chloroform), and the eluent obtained from this mobile phase cannot be directly combined with mass spectrometry equipment

Method used

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  • Hydrophilic interaction chromatography-tandem mass spectrometry detection method of phospholipids in Metapenaeus ensis
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  • Hydrophilic interaction chromatography-tandem mass spectrometry detection method of phospholipids in Metapenaeus ensis

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Embodiment 1

[0059] Embodiment 1, a kind of hydrophilic chromatography-tandem mass spectrometry detection method of phospholipids in Penaeus penaeus

[0060] Specifically, the following steps are carried out in sequence:

[0061] 1), sample pretreatment:

[0062] After peeling and excreting the excretory glands of Penaeus lanceolata, take the abdominal striated muscle tissue as shrimp meat and grind it into minced shrimp (paste). Weigh 1.0g minced shrimp into a 5mL centrifuge tube, add 18mL chloroform / methanol mixture (1:2, v / v) and shake to mix. Ultrasonic (25kHz) ice-bath extraction at 250W for 10 minutes, then add 12.5mL ultrapure water to the centrifuge tube and shake to mix. The mixture was centrifuged at 8000 g for 5 minutes at 4° C. with a refrigerated centrifuge (Thermo Scientific, USA). After centrifugation, the solution in the centrifuge tube was separated into upper and lower layers, and the tissue sample was in a cake shape in the middle of the two phases. Use a pipette gun ...

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Abstract

The invention discloses a hydrophilic interaction chromatography-tandem mass spectrometry detection method of phospholipids in Metapenaeus ensis. The method includes the steps of firstly, performing corresponding preprocessing on ground Metapenaeus ensis to obtain crude lipid extract; secondly, performing liquid phase separation on the crude lipid extract, wherein a chromatographic column is a YMC Triart diol HILIC column, a gradient elution method is used, a gradient system comprises a flowing phase A and a flowing phase B, the flowing phase A is an acetonitrile solution containing 53mmol / L formic acid, and the flowing phase B is an aqueous solution containing 60mM ammonium formate and 53mM formic acid; thirdly, performing mass spectrometric detection analysis on the elution liquid obtained in the second step. By the method, the total content of PE, PS and PC in the Metapenaeus ensis can be detected accurately, and mass spectrometry identification of various molecular species in the PC, PE and PS phospholipids can be achieved.

Description

technical field [0001] The invention belongs to the field of food testing, and relates to a method for detecting phospholipids in Penaeus penaeus by hydrophilic chromatography-tandem mass spectrometry, specifically referring to three major categories of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine in Penaeus penaeus Methods for structural identification and quantitative analysis of phospholipids. Background technique [0002] The new prawn (Metapenaeus ensis), that is, the base shrimp, is one of the small-scale marine shrimps cultured in my country. Because of its advantages such as miscellaneous food habits, fast growth, high temperature resistance, wide salt range, and strong resistance to adverse environments, artificial breeding has been widely carried out in the central and southern coastal areas in recent years, showing good economic prospects. Shrimp aquatic resources are high in lipid content, rich in omega-3 and omega-6 polyunsaturated fatty...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 沈清戴志远冯俊丽金仁耀陈康薛静
Owner ZHEJIANG GONGSHANG UNIVERSITY
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