133 results about "Schizochytrium" patented technology

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

The invention relates to a method for producing triglyceride type docosahexenoic acid fat by industrially fermenting schizochytrium. The method comprises the following step: the docosahexenoic acid fat is prepared by fermenting the schizochytrium taken as the strain with the a culture medium with microelements; wherein the culture medium with the microelements comprises a carbon source, a nitrogen source, inorganic salt and microelements, and the microelements comprise at least one of biotin, chlorophyll, beta-carotene, vitaminB1, vitaminB6 and vitaminB12. The prepared docosahexenoic acid fat does not need to be further chemically modified and can be directly used by people needing DHA, including infants, pregnant women, breast feeding women or patients who suffer from diseases due to insufficient DHA and need to supplement DHA. The invention has the advantages of high DHA content, high purity, safe and reliable quality, low production cost, stable production and almost no EPA; and the environmental-friendly, safe and high-content triglyceride type DHA fat can be produced with low cost at a large scale.

The invention discloses a method for producing DHA by Schizochytrium in high-density culture through fermentation, relates to a preparation method of DHA, and provides a method for producing DHA by using a microbial fermentation method, which replaces fish oil to extract omega-3 polyunsaturated fatty acids. The method comprises the following steps of: inoculating strains in a shake flask according to the ratio of strains to a culture medium of 1: (5-10) for culturing until the dry weight of cells in fermentation liquor is 10-12g / L; inoculating the strains in the shake flask in a 1000L seed fermenter according to the inoculum size of 5-10%, culturing until the dry weight of the cells in the fermentation liquor is 20-24g / L; inoculating the strains in the 1000L seed fermenter in a 8000L seed fermenter according to the inoculum size of 5-10%, and culturing for 120-130 hours, wherein the dry weight of the cells in the fermentation liquor is 120-150g / L, and the DHA content is up to 26-30g / L. In the invention, through optimizing the culture medium and optimizing the culture conditions and by adopting a conventional fermentation method, the production rate of the DHA is at least 3 times more than the reported production rate. The production costs of the strains (the Schizochytrium), the culture medium and the culture condition are low, the yield is high, and the method is applicable to the industrial production of the DHA.

The invention provides a fermentation production technique of schizogenesis chytrid, which solves the problems of lower yield and higher production cost in the prior art. The fermentation production technique has the technical scheme that bean pulp is taken as main nitrogen source for fermentation production, and the technique comprises the steps of bean pulp hydrolysis, pressing filter, preparation of fermentation medium, arrangement of a fermentation tank system, collection and drying of the schizogenesis chytrid, and preparing the schizogenesis chytrid dry powder. The new fermentation production technique of the schizogenesis chytrid takes the bean pulp as main nitrogen, thus not only reducing the cost of fermentation production, but also improving the growth rate of the schizogenesis chytrid and promoting fatty acid accumulation of the cells of the schizogenesis chytrid.