The invention relates to a formula of a fruit and vegetable beverage and application thereof, in particular to a formula which is beneficial to eye nutrition and vision care and is prepared from Chinese wolfberry juice, mulberry juice, carrot juice and other additives. The formula can be added with one or more of vitamin A, beta-carotene, vitamin C, vitamin B1, vitamin B2, vitamin B6, vitamin B12, lutein and lutein ester; the formula can be added with one or more trace elements of zinc, selenium, manganese, chromium, calcium, phosphorus and molybdenum; the formula can be added with one or more of blueberry juice, orange juice, strawberry juice and cherry juice; and the formula can be added with one or more Chinese medicinal extracts of chrysanthemum, kudzuvine root, glossy privet fruit, mulberry leaf, pearl, ginkgo leaf, fiveleaf gynostemma herb, anthocyanin, grape seed, cassia seed, south dodder seed and feather cockscomb seed.