105 results about "Scenedesmus" patented technology

Materials and methods are provided for growing algae while maintaining culture selectivity. Algae that can be grown include, for example, green algae such as those of the genus Scenedesmus. Lipid obtained from the algae can be used to produce biofuels such as biodiesel or polyunsaturated fatty acids such as omega-3 fatty acids. Feedstocks such as animal feed and aquaculture feed can also be produced as can phytonutrients such as asataxanthin and beta-carotene.

The invention discloses a device and a method for coupling producing biological oil by utilizing microalgae to deeply treating wastewater, which belongs to the technical field of water pollution control. The device is arranged after secondary wastewater treatment, and nutrient elements of nitrogen, phosphorus and the like can be absorbed by the photosynthesis of algae cells, and thus, the nitrogen and phosphorus concentration in secondary wastewater treatment effluent can be further reduced, thereby lowering the risk of water bloom eruption because the sewage plant effluent is discharged behind scenic water; the device can be applied to the deep treatment of the wastewater. The growth rate of the algae cells and the nitrogen and phosphorus removal rate can be increased by taking Scenedesmus sp.LX1 as a nitrogen and phosphorus removal dominant algae by adopting an LED red and blue light source; and the algae density in a reactor and the nitrogen and phosphorus removal can be increased by adopting a membrane separation mode and a dense algae liquid reflux mode. Meanwhile, grease can be further extracted by separating the obtained dense algae liquid, and the wastewater is taken as the resource to produce the biological oil. The method has the advantages of good nitrogen and phosphorus removal effect, high economic benefit and the like.

The invention discloses a composite bacteria-algae preparation, which consists of nitrifying bacteria, bacillus licheniformis, scenedesmus obliqnus and selenastrum capricornutum, wherein the volume ratio of the nitrifying bacteria to the bacillus licheniformis to the scenedesmus obliqnus to the selenastrum capricornutum is 1-2: 1-3:1-3:1-3, and the microbe inoculation amount per milliliter is 8*106CFU. The composite bacteria-algae preparation also can be prepared into a globular immobilized preparation by adopting a known embedding method, using the nitrifying bacteria, the bacillus licheniformis, the scenedesmus obliqnus and the selenastrum capricornutum as embedding bacteria and using polyvinyl alcohol, sodium alginate, active carbon, silicon dioxide and calcium carbonate as embedding agents. The bacteria, algae and immobilization materials used by the composite bacteria-algae preparation have no toxic or side effect on aquaculture animals, and can effectively purify the aquaculture waste water; the bacteria and the algae can inhibit the growth of harmful pathogenic bacteria to a certain degree; an immobilization method has simple operation; the immobilized efficient bacteria-algae microbial bactericide is easy to store and transport; and the immobilization materials are economic and practical and easy to recycle.

The invention provides a method for detecting microalgae oil, belonging to the field of oil detection. The method solves the technical problems that the needed sample is large and the consumed time is long in the existing weighing method. The method comprises the following steps: 1, diluting microalgae culture solution to obtain diluent; 2, detecting the fluorescence intensity of the dyed diluent; 3, centrifuging the diluent, and detecting the fluorescence intensity of the dyed supernate; 4, detecting the fluorescence intensity of the undyed diluent; and 5, computing the maximum difference of the fluorescence intensity, and computing the content of the microalgae oil according to a fluorescence intensity-oil content standard curve. The method is applied to detecting the unicellular algae such as the chlorella, the scenedesmus, the spirulina, the diatom, the dinoflagellate, the chrysophyceae, the euglenophyta, the stonewort and the like, and is applied to detecting the macro algae which is preprocessed by cell discruption.

The invention relates to a method for treating waste water, in particular to a biological treatment method for efficiently purifying biogas slurry in a pig farm through composite microalgae, and belongs to the technical field of biological environmental protection.According to the method, the composite microalgae containing scenedesmus and haematococcaceae are prepared, the biogas slurry in the pig farm is treated through the composite microalgae according to the inoculation amount of 15% to 20% (by volume), the treatment temperature ranges from 24 DEG C to 26 DEG C, the pH value ranges from 7.0 to 8.0, and illumination intensity ranges from 4,000 lux to 6,000 lux.The synergistic effect of two kinds of microalgae is utilized, the defect that the treating effect of a single alga species on the biogas slurry is insufficient is overcome, then the utilization rate of organic matter, ammonia nitrogen, phosphorus and other matter in the biogas slurry in the pig farm increased through the microalgae, and therefore the treating and purifying efficiency of the composite microalgae on the biogas slurry is improved.Compared with treatment of the biogas slurry through a single alga species, the removal efficiency of the composite microalgae on ammonia nitrogen in the biogas slurry is improved by 8%, and the removal efficiency on phosphorus is improved by 15%.According to the method, the method for treating the biogas slurry is provided, the microalgae obtained after treatment can be collected and recycled, ecological treatment of the biogas slurry is achieved, and economic and social benefits are generated while the environment is improved.

The invention discloses low-nutrition scenedesmus sp. LX1 in the technical field of water pollution control and the application thereof in sewage deep treatment. Firstly, the scenedesmus is inoculated in second-grade effluent water of municipal sewage, and through the photosynthesis of algae cells, the scenedesmus absorbs nutrient elements in the second-grade effluent water such as nitrogen, phosphorus and the like in the process of growth, thus further reducing the concentration of nitrogen and phosphorus in the effluent water of a municipal sewage treating plant. As the scenedesmus can grow in low-nutrition environment, the nitrogen and phosphorus in the second-grade effluent water can be removed to a quite low level, thus reducing the risk of water bloom breakout to the maximum extent; the scenedesmus is used for removing the nitrogen and phosphorus from the second-grade effluent water of municipal sewage, which is a sewage deep treatment method with eco-friendliness, low cost, high economic efficiency and easy use, and the method has the advantages of easy cultivation of the algae cells, good effect of removing the nitrogen and phosphorus and the like.

The invention provides a scenedesmus strain (Desmodesmus sp.) and application of the scenedesmus strain. The scenedesmus strain is ENN2203A and stored in the general microbe center of China microbe culture preservation management council on November 11, 2012. The preservation number is CGMCC NO.4323. The scenedesmus strain has the advantages of being high temperature resistant, easy to collect, strong in environmental suitability, suitable for high density cultivation, and capable of being used for treatment of sewage containing nitrogen and phosphorus and carbon dioxide emission reduction, and is used for biodiesel production or used for baits of an aquatic product or feeding stuff of animal. Industrialization utilization potential is relatively high.

The invention discloses a strain of scenedesmus sp. as well as a culture method and application thereof. The strain is HCS-02, is classified and named as scenedesmus sp., is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 24, 2015, and has a preservation number of CGMCC No. 10766. The scenedesmus sp. can tolerate lower culture temperature, is large in culture temperature interval and can be easily cultured in an open type. The scenedesmus sp. shows a rapider biomass accumulation speed under different culture conditions; the obtained biomass is rich in starch and oil, thus being used as feed or used for producing biodiesel after being treated.

The invention relates to a method for producing microalgae grease by using flue gas. The method for the producing microalgae grease by using the flue gas comprises the following steps that firstly, amicroalgae culture medium and parachlorella kessleri FSH-Y3 or / and a scenedesmus obliquus FSH-Y2 seed solution are added into a photobioreactor, the pH value is adjusted to 10-12, the flue gas with CO2 content of 1v-5v% is introduced, and culture is carried out for a certain time; and then the pH value is adjusted to 8-10, a chlorella SF-B1 seed solution is introduced, at least one of a scenedesmus MH-04 seed solution a monoraphidium SS-B1 seed solution is introduced, mixed culture is carried out, the flue gas with CO2 content of 5v%-45v% is introduced, and the mixture is cultured to a stableperiod under the condition of continuous illumination, so that microalgae cells are obtained. The method for producing the microalgae grease by using the flue gas improves the tolerance and solubilityof a microalgae culture system to high-concentration CO2, improves the carbon fixation efficiency, obviously improves the harvest amount of the microalgae grease, simultaneously improves the tolerance to SOx and NOx in the flue gas, and can purify the flue gas.

The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chlorella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.

Materials and methods are provided for growing algae while maintaining culture selectivity. Algae that can be grown include, for example, green algae such as those of the genus Scenedesmus. Lipid obtained from the algae can be used to produce biofuels such as biodiesel or polyunsaturated fatty acids such as omega-3 fatty acids. Feedstocks such as animal feed and aquaculture feed can also be produced as can phytonutrients such as asataxanthin and beta-carotene.

The invention discloses an organic freshwater shrimp bait which comprises the following components in parts by weight: 10-15 parts of animal manure, 10-15 parts of chicken manure, 10-15 parts of cow dung, 10-15 parts of pig manure, 8-12 parts of straws, 8-12 parts of vegetable leaves, 8-12 parts of rice bran, 8-12 parts of bean cakes, 8-12 parts of rapeseed cakes, 8-12 parts of internal organs of animals, 2-5 parts of green alga, 2-5 parts of scenedesmus, 2-5 parts of navicula tenera, 2-5 parts of dinoflagellate, 2-5 parts of tetraedron minimum, 2-5 parts of spindle diatom, 2-5 parts of asterionella, 2-5 parts of wheel animalcule, 2-5 parts of flagellate and 2-5 parts of euglena viridis. According to the mode, the freshwater shrimp organic bait disclosed by the invention has the advantages of being natural, free of pollution, wide in source, sufficient in nutrition, free of hormone and the like and has wide market prospects in popularization.

The invention discloses scenedesmus bijuga capable of producing oil in high yield as well as a screening and culture method and application thereof. A algae specie, which is obtained by separating from a polluted water area is identified by virtue of 18SrDNA sequence analysis and the microscopic morphology to determine that the algae is a member of Scenedesmus sp., has a serial number of KJ413176. The algal strain is named as scenedesmus bijuga SDEC-12 and is collected in China Center for Type Culture Collection (CCTCC) in October 2014 and has the collection number of CCTCC NO: M 2014497. By virtue of using artificially prepared domestic sewage as an alternative culture medium, the yield and productivity of oil produced by scenedesmus bijuga SDEC-12 are significantly higher than those of the oil produced by scenedesmus bijuga SDEC-12 which is cultured by the commonly used BG-11 culture medium; the extracted fatty acid methyl ester component analysis shows that scenedesmus bijuga SDEC-12 cultured by the domestic sewage culture medium has the characteristics of better oxidative stability and low-temperature fluidity and therefore the scenedesmus bijuga SDEC-12 is more suitable for serving as a raw material for biodiesel. Scenedesmus bijuga SDEC-12 grows and simultaneously absorbs nitrogen and phosphorus nutrient salts in the domestic sewage so that the effective treatment of the domestic sewage is achieved and the domestic sewage is used as the alternative culture medium to achieve both economic and environmental benefits.

The invention belongs to the field of microalga culture and relates to a heterotrophic culture method of microalgae such as scenedesmus. The method is characterized in that ammonium salt is used as the basic nitrogen source in a heterotrophic culture medium, and ammonia water is used as the pH regulator and / or supplementary nitrogen source. By the method, the biomass of the microalgae such as the scenedesmus can be increased effectively.

The invention discloses a method for treating high-nitrogen and low-carbon rare earth ore mining wastewater by using microalgae and activated sludge and belongs to the technical field of sewage treatment. The method for treating rare earth ore mining water comprises the following steps: 1) regulating the ratio of nitrogen and phosphorus in the rare earth ore mining wastewater to (0.5-1.5):(2-3) byusing a phosphorus-containing compound to obtain to-be-purified wastewater; 2) adding enriching microalgae into the to-be-purified wastewater to carry out purification treatment, and separating algaefrom water to obtain primarily-purified water; and 3) adding activated sludge into the primarily-purified water to carry out purification treatment again to obtain purified water. The wastewater is treated by using scenedesmus and chlorella in the method for treating high-nitrogen and low-carbon rare earth ore mining wastewater by using microalgae and activated sludge, so that the contents of ammonia nitrogen and total nitrogen in the wastewater can be effectively reduced, COD additionally added during microalgae treatment is subjected to purification treatment again by the activated sludge,so that the COD value is reduced to 32mg / L, and the wastewater meets a discharge requirement of the first level A of the discharge standard for pollutants from an urban wastewater treatment plant.

Compositions and methods for indirectly reducing incidence of fungal pathogen infection in a plant are disclosed, wherein the fungal pathogen may be Botrytis, Macrophomina, Rhizoctonia, Sclerotinia, or Verticillium. The compositions comprise pasteurized microalgae cells in an amount effective to reduce incidence of the fungal pathogen in a plant compared to a substantially identical population of untreated plants. The composition treatment can include at least one microalgae such as Chlorella, Aurantiochytrium acetophilum HS399, Galdieria, Scenedesmus, Haematococcus, Isochrysis, Spirulina or any combination thereof. Where the composition comprises a combination of a first microalgae and a second microalgae, the composition may consist of a ratio of 25:75, 50:50: or 75:25.

The invention discloses a method for wall breaking of oil-containing microalgae and assistant assisted extraction of oil. The method comprises the following steps: 1, adjusting the pH value of a suspension of the oil-containing microalgae to 7.5-14, and carrying out hydrothermal treatment; 2, carrying out an enzymatic hydrolysis reaction in the presence of an enzyme; 3, adding an assistant for promoting precipitation of oil in the oil-containing microalgae, and reacting; and 4, adding an organic solvent to extract, and separating the oil. The method has the advantages of high oil extraction rate, mild operation conditions, effective maintenance of nutrition components of the microalgae, small dosage of the organic solvent, environmental protection and low energy consumption, is suitable for Nannochloropsis oceanica, chlorella, diatom, scenedesmus, Monoraphidium sp. and other microalgae, and has a wide application range.

The invention relates to mixotrophic scenedesmus and an application in sewage resource treatment. The scenedesmus has strong anti-pollution capability and can grow stably in actual sewage (non-sterile), the heterotrophic capability is strong, the productivity of biomass and grease is high, namely organic matters in the sewage can be transformed to grease, nutrient substances in the sewage can be further utilized for absorbing carbon dioxide in atmosphere, nitrogen and phosphorus in the sewage can be removed in a sustained manner under the situation that an organic carbon source is used up, and when the sewage is taken as a matrix for inoculating the scenedesmus, the water quality can be purified, and the grease can be simultaneously accumulated, so that sewage resources can be effectively utilized, the waste can be changed to valuable things, and the mixotrophic scenedesmus further has the advantages of low operation cost, good quality of outlet water, simple and convenient technology, easiness in popularization and the like.

The present invention discloses a strain of Scenedesmus sp. and applications thereof. The Scenedesmus sp. has advantages of fast growth, rapid grease accumulation, high oil content, effective removal of nitrogen-containing compounds in water, and the like, contains a suitable part of useful pigments, and can be used for productions of biodiesel, food, health care products, feeds, aquatic product baits, drugs and cosmetics.

The invention relates to scenedesmus capable of highly yielding oil as well as a culture method and application thereof. An alga variety separated from a eutrophic water region is subjected to 18S rDNA sequence analysis and microstructure morphology identification so that the alga is determined as the member of Scenedesmus sp. and the sequence number is KF999043. The alga strain is named as Scenedesmus quadricauda SDEC-8 and is preserved in China Center for Type Culture Collection on October 8, 2014; and the preservation number is CCTCC NO: M2014448. An oil production culture method comprises the following steps: separating single alga cells of the Scenedesmus quadricauda SDEC-8 and carrying out aeration enlarged cultivation on obtained pure cells under laboratory conditions. The Scenedesmus quadricauda SDEC-8 contains rich high-quality oil; and the oil content can be up to 31.8% of the dry weight of the cells and the content of saturated fatty acids in fatty acids can be up to 80%, so that the Scenedesmus quadricauda SDEC-8 can be used as the raw material of biodiesel. Based on a high cetane number (61.38) and a low iodine number (29.38gI2 / 100g), the biodiesel prepared by taking the microalga as the raw material has good oxidization stability.

The invention discloses high-oil-yield scenedesmus SDEC-13 and a culture method and an application thereof. An algal strain disclosed by the invention is scenedesmus quadricauda SDEC-13, and can grow by virtue of high-concentration CO2; a gene sequence is submitted to a GenBank database; the number is KF999644; the algal specie is preserved at the China Center for Type Culture Collection in November 2014; and the preservation number is CCTCC M4014498. According to the algal specie disclosed by the invention, high-concentration CO2 in the waste gas can be utilized to reduce the culture cost. The scenedesmus disclosed by the invention is rich in excellent oil; the total oil content can reach 27.2% of dry weight; the saturated fatty acid content in fatty acid can be up to 82.87%; the content of the fatty acid of which the carbon chain length is 16-18 in fatty acid components can be up to over 98%; and the scenedesmus is suitable for being used as a raw material for producing biodiesel.

The invention discloses a wall-breaking and surfactant-assisted method used for extracting grease from microalgae containing oil. The wall-breaking and surfactant-assisted method comprises following steps: (1) pH value of a suspension liquid of microalgae containing oil is adjusted to be 7.5 to 14, and is subjected to hydro-thermal treatment; (2) after hydro-thermal treatment, enzymatic hydrolysis is carried out in the presence of enzymes; and (3) after enzymatic hydrolysis, a surfactant is added for reaction, and an organic solvent is added for extraction and separation of grease. Grease extraction rate of the wall-breaking and surfactant-assisted method is high; operation conditions are mild; nutritional ingredients of microalgae can be maintained effectively; using amount of the organic solvent is small; the wall-breaking and surfactant-assisted method is friendly to the environment, is low in energy consumption, and is suitable for nannochloropsis oculata, chlorella, diatom, scenedesmus, and monoraphidium komarkova-legnerova; and application range is wide.

The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chlorella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.

The invention discloses a method for preparing microalgae oil. The method comprises the following steps: adding a microalgae culture medium and a mixed microalgae seed solution to a photobioreactor, keeping the pH value of a culture system to be 8-12 and introducing gas which contains 5-45v% of CO2 by volume, wherein the mixed microalgae includes scenedesmus obliqnus FSH-Y2 and monoraphidium sp SS-B1 which are preserved in China General Microbiological Culture Collection Center on September 11, 2012 and April 15, 2013 by preservation numbers of CGMCC No.6551 and CGMCC No.7479, respectively. By virtue of the method disclosed by the invention, the solubility and the tolerance of the microalgae culture system to the high-concentration CO2 are improved, a carbon sequestration efficiency is improved and the yield of the microalgae oil is obviously improved, and the obtained microalgae oil can be used for producing biodiesel.

The invention discloses Scenedesmus sp., CHX1 and a use thereof. The Scenedesmus sp., CHX1 is separated from a pig feeding wastewater treatment tank, is named as CHX1, has a class name of Scenedesmus sp., is preserved in the China general microbiological culture collection center and has a preservation number of CGMCC No. 6649. A Scenedesmus sp., CHX1-optimized medium is obtained by preparing solutes of 1ml of an A5+Co solution, 5g of glucose, 3g of NaNO3, 0.04g of K2HPO4.3H2O, 0.075g of MgSO4.7H2O, 0.036g of CaCl2.2H2O, 0.006g of Fe(NH4)3Cl8H10O14, 0.001g of Na2-EDTA, and the balance water into 1L of a solution. The Scenedesmus sp., CHX1 is cultured into a seed culture solution by the Scenedesmus sp., CHX1-optimized medium. The seed culture solution can be used for preparation of biodiesel and treatment on pig feeding wastewater. The Scenedesmus sp., CHX1 can grow well in pig feeding wastewater, can efficiently remove nitrogen and phosphor in wastewater, has a high biological yield and an oil yield, and is suitable for biodiesel production. A fatty acid produced by the Scenedesmus sp., CHX1 has C16-C18 content more than 70%.

The invention provides application of artemether as an algae inhibiting agent. Artemisinin simple ether derivatives have a good inhibition effect on copper aluminum microcystis, scenedesmus obliqnus and the like. The artemisinin simple ether derivatives can be prepared into missible oil, clathrate, slow release microcapsules and the like as algae inhibiting agents applied in nature water bodies like ponds, reservoirs and lakes for inhibiting algae booms.

The present invention relates to microalgae having high efficiency and ability to remove carbon dioxide, and the use thereof. More specifically, the invention relates to Scenedesmus producto-capitatus microalgae having high carbon dioxide fixation rate and the use thereof. The microalgae of the invention have a high carbon dioxide fixation rate and excellent resistance even to high concentrations of carbon dioxide, sulfur oxide (SOx) and nitric oxide (NOx) and are rich in biomass, and thus can be advantageously used in formulations for removing carbon dioxide.

The invention discloses a liquid foundation with anti-blue light and color change effects and a preparation method of the liquid foundation. The liquid foundation comprises an anti-blue light composition and a color change composition, wherein the anti-blue light composition comprises, in terms of the total mass of the liquid foundation, 8-10% of scenedesmus carotene, 1-4% of a rosemary leaf extract and 5-8% of hyaluronic acid; and the color change composition comprises 0.1-0.3% of colored power with different colors. The liquid foundation can effectively reduce injury of blue light to skin, the color change composition can change colors of the liquid foundation, and a user can adjust required colors according to the skin color of the user.

The present disclosure relates to methods of transforming various species of algae, for example, algae from the genus Scenedesmus and the genus Dunaliella, vectors and nucleic acid constructs useful in conducting such transformations, and recombinant algae, for example, Scenedesmus and Dunaliella produced using the vectors and methods disclosed herein.

The invention provides a culture method with the advantages that the growth speed of the rich-oil scenedesmus obliqnus is improved; the cell density is increased; the culture time is shortened; the grease accumulation is promoted. Bioflocculant fermentation bacterium solution containing various composite flocculant-producing bacteria is added into a culture medium for culturing scenedesmus obliqnus. The method has the advantages that the scenedesmus obliqnus cells can fast grow; the cell density of the cultured scenedesmus obliqnus is great; particularly, due to the addition of the bioflocculant fermentation bacterium solution, the fast proliferation of the scenedesmus obliqnus cells can be promoted, so that the scenedesmus obliqnus cell concentration is increased; the culture time is shortened; the culture cost is further reduced; the grease content is obviously increased. The culture method provided by the invention is particularly suitable for large-batch scaled culture production.