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Scenedesmus sp. and applications thereof

A common technology for Scenedesmus, applied in the field of microorganisms, can solve the problems of low processing capacity and performance, and achieve the effects of high CO2 utilization rate, wide temperature tolerance range, and fast growth rate

Active Publication Date: 2013-03-20
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although microalgae have very good development prospects in energy, environmental protection and even medicine, and related research work has been gradually carried out, the currently known microalgae, especially Scenedesmus, usually have an oil content below 40%, and are resistant to CO 2 The performance in terms of processing capacity and extreme environmental tolerance is still generally low

Method used

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  • Scenedesmus sp. and applications thereof
  • Scenedesmus sp. and applications thereof
  • Scenedesmus sp. and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 screening Scenedesmus strain

[0040] Take the soil sample taken from the Ulanqab area of ​​Inner Mongolia after dilution with sterile water, and add BG11 medium for enrichment culture. After observation under a 400-fold microscope, the approximate cell concentration is 800-1200 cells / ml. 1 ul of algae liquid was inoculated in a 48-well culture plate equipped with BG11 medium, and cultured at 25°C with a light intensity of 50umol / m2.s. When a certain cell concentration was reached, observe with a microscope and select only single algae Strain wells were diluted to a cell concentration of 1000-3000 cells / ml and plated to obtain a single algae strain.

Embodiment 2

[0041] Embodiment 2 identifies algal species and its classification on the evolutionary tree

[0042] Identification of algae strains takes a combination of morphological identification and molecular identification.

[0043] 1. Shape identification (see figure 1 ): The isolated algae strain ENN32 was observed under a 1000-fold microscope, and the algal body was in the form of a single cell, the cell wall was smooth, and the single cell was oval. The long diameter of the cells is 8-10 μm, and the short diameter is 6-8 μm. Chromosomes are perinatal, flaky, with a pyrenoid, and produce spore-like spores through asexual reproduction. Retrieval of "Freshwater Algae in China - System, Classification and Ecology", found that the algae are classified in the same form as Chlorophyta, Chlorophyceae, Chlorococcales, Scenedesmaceae ), Scenedesmus.

[0044] 2. Molecular identification:

[0045] A, Amplification of its ITS and 18S partial nucleotide sequence from isolated ENN32

[004...

Embodiment 3

[0072] Indoor culture and oil accumulation of embodiment 3ENN32 Scenedesmus

[0073] Inoculate the green swimming cells of Scenedesmus strain ENN 32 in the logarithmic growth phase in the prepared BG11 medium (see Table 1 for the formula) to make the cell density reach OD 750 It is between 0.2-0.8. During the cultivation process, the light intensity is controlled at 50-500umol / m 2 .s, during the culture period, adjust the pH value of the culture medium between 7-9 and the temperature within the range of 15-35°C by feeding a mixture of 1.5-2% carbon dioxide and air into the culture medium . The reactor used for the cultivation was a column reactor with an inner diameter of 40 mm and a length of 600 mm. During the cultivation process, samples were taken regularly to determine the dry weight, and the results were shown in Figure 4 . On the 16th day of cultivation, when the color of the algae liquid turns yellow, the algae liquid is collected, and the algae mud is obtained b...

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Abstract

The present invention discloses a strain of Scenedesmus sp. and applications thereof. The Scenedesmus sp. has advantages of fast growth, rapid grease accumulation, high oil content, effective removal of nitrogen-containing compounds in water, and the like, contains a suitable part of useful pigments, and can be used for productions of biodiesel, food, health care products, feeds, aquatic product baits, drugs and cosmetics.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to Scenedesmus ENN32 (Scenedesmus sp.) and its application. Background technique [0002] With the gradual depletion of the world's fossil fuel reserves, the continuous increase of carbon dioxide emissions and the increasing warming of the global climate, human survival and development have faced unprecedented challenges. Therefore, countries all over the world are actively looking for renewable energy that can replace petroleum products. Among them, biodiesel is an important bioenergy. However, common oil crops such as corn and soybeans have a long cultivation cycle and occupy more farmland. The problem of "competing with people for food and land with food" will arise, which will lead to the embarrassing result of "the energy crisis has been solved, but the food crisis has emerged". [0003] Microalgae is a kind of low-level plants that are widely distributed on land and in the ocean...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P7/64C12P7/06C09B61/00C02F3/34B01D53/84B01D53/62A61K36/05A61K8/99A23L1/30A23K1/00A01K97/04C12R1/89
CPCY02E50/17Y02E50/13Y02A50/20Y02E50/10Y02P20/151Y02P20/59Y02W10/37
Inventor 罗少敬邓平张晓楠吴洪王冰
Owner ENN SCI & TECH DEV
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