40 results about "Monoraphidium" patented technology

The present invention disclosed the obtaining and application of a single -needle algae LB59, which involves the screening and cultivation technology of oil -producing microalgae; the algae species obtained from the separation of the field environment are analyzed and identified by 18srdna sequences, and the algae is determined that the algae is a single needle.Members of Monoraphidium, the sequence is seqno.1.Monopolysis LB59 can tolerate high -concentration of sodium bicarbonate, easy to carry out open culture, rich in oil. In the case of maintaining the growth and collection rate of 23.6g / m2D%, Fatty acids contain more than 20%18 carbon 3 ole acid and 18 carbon 4 ole acid.

The present invention provides a joint method of cultivating microalgae combined with denitrating an industrial waste gas and a system useful for the same. The joint method comprises the steps of: (1) a step of cultivating microalgae; (2) a separation step of separating a microalgae suspension obtained from step (1) into a wet microalgae (microalgae biomass) and a residual cultivation solution; (3) a NOx absorbing / immobilizing step of denitrating an industrial waste gas with the residual cultivation solution obtained from step (2); wherein the nutrient stream absorbed with NOx obtained from step (3) is used to provide nitrogen source to the microalgae cultivation of step (1). During the microalgae cultivation, EM bacteria is added into the microalgae suspension. The microalgae is preferably Chlorella sp., Scenedesmus sp., Monoraphidium sp. or Spirulina sp.

The invention discloses a monoraphidium sp. strain SS-06 with high yield of starch and fat, wherein the classification name of the monoraphidium sp. strain SS-06 is Monoraphidium sp., and the monoraphidium sp. strain SS-06 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on April 24, 2015, and is assigned with the accession number of CGMCC No.10765. The bred monoraphidium sp. strain SS-06 has good application potential, is wide in environmental adaptation, and can produce starch and fat with high yield under the suitable condition, therefore, the different demands of the downstream production can be satisfied, and the application range of the monoraphidium sp. strain SS-06 is expanded.

The invention discloses a method for producing starch by Monoraphidium. The method comprises (1) preparing a microalgae seed liquid, wherein the microalgae is Monoraphidium sp. SS-06 and has a preservation number of CGMCC No. 10765, (2) inoculating an N element-rich microalgae culture medium with the Monoraphidium seed liquid, carrying out culture until a logarithmic growth phase, carrying out standing and sedimentation, discharging the supernatant and acquiring microalgae cells, (3) inoculating a low N element content microalgae culture medium with the microalgae cells, adding an ADP glucose pyrophosphatase inhibitor into the culture medium and carrying out continuous illumination culture until a stable period to obtain starch-rich microalgae cells. The method is conducive to proliferation of microalgae cells and accumulation of starch, can produce microalgae cells with high starch content and has the characteristics of simple processes, low cost and high yield.

The invention relates to the field of microalgae biology, and discloses a culture method for monoraphidium. The method includes (1) subjecting the monoraphidium to culture of a biomass increasing stage; (2) when the OD680 value of an alga solution is 0.01-10, separating the alga solution to obtain a separated clear liquid I and monoraphidium mud, and returning the separated clear liquid I to the step (1) for reuse; (3) subjecting the monoraphidium mud obtained in the step (2) to culture of an oil and grease accumulating stage, with the culture manner being nitrogen starvation stress culture; (4) separating after 5-10 days of stress culture to obtain a separated clear liquid II and monoraphidium with accumulated oil and grease, and returning the separated clear liquid II to the step (3) for reuse; and (5) drying the monoraphidium with accumulated oil and grease to obtain a monoraphidium product. According to the method, operation of a culture process is stable, quality control is easy, and all culture liquids in the culture process can be recovered and reused so that the culture cost is low and no environment pollution is generated.

The invention discloses a method for producing microalgae lipid by virtue of flue gas. The method comprises the following steps: (1) adding a microalgae medium and a parachlorella kessleri FSH-Y3 seed solution to a photobioreactor, regulating pH value of a culture system to 10-12, supplying the flue gas that a CO2 volume content is 1-5v%, and conducting culture for 2-5 days; and (2) regulating the pH value of the culture system to 8-10, inoculating the culture system with either a desmodesmus sp. MH-04 seed solution or a monoraphidium sp. SS-B1 seed solution or both of them, simultaneously, inoculating the culture system with an ankistrodesmus sp. SS-B7 and implementing mixed culture, and supplying the flue gas that a CO2 volume content is 5-45v%; implementing culture to a stationary phase under a continuous illumination condition, and collecting microalgae cells. According to the method provided by the invention, the tolerance and the solubility of the microalgae culture system to high-concentration CO2 are improved and a carbon fixation efficiency is improved, meanwhile, tolerance to SOx and NOx in the flue gas is improved, and the yield of the microalgae lipid is obviously improved.

The invention discloses a method for producing microalgal oil by using flue gas, wherein the method includes the steps: (1) adding a microalgae culture medium and a scenedesmus obliquus FSH-Y2 seed liquid into a photobioreactor, regulating the pH of the culture system to 10-12, introducing flue gas with the CO2 volume content of 1 v%-5 v%, and culturing for 2-5 days; and (2) regulating the pH value of the culture system to 8-10, inoculating with monoraphidium sp. SS-B1 and ankistrodesmus sp. SS-B7 seed liquids, carrying out mixed culture, introducing flue gas with the CO2 volume content of 5 v%-45 v%, culturing to a stable stage under continuous illumination, and harvesting microalgae cells, wherein the scenedesmus obliquus FSH-Y2, the monoraphidium sp. SS-B1 and the ankistrodesmus sp. SS-B7 are respectively preserved in China General Microbiological Culture Collection Center on September 11, 2012 and on April 15, 2013 respectively and have the preservation numbers of CGMCC No.6551, CGMCC No.7479 and CGMCC No.7478 respectively. The method improves the tolerance and the solubility of the microalgae culture system on high-concentration CO2, improves the carbon sequestration efficiency, obviously increases the yield of the microalgal oil, and can realize purification of the flue gas.

The invention belongs to the technical field of new bio-energy, and discloses a process for preparing biodiesel by using Monopodella cells. algae, step 2) oil extraction, step 3) biodiesel production. The biodiesel prepared by the process of the invention meets relevant standards, has high yield and low cost.

The invention discloses a method for preparing microalgae oil. The method comprises the following steps: adding a microalgae culture medium and a mixed microalgae seed solution to a photobioreactor, keeping the pH value of a culture system to be 8-12 and introducing gas which contains 5-45v% of CO2 by volume, wherein the mixed microalgae includes scenedesmus obliqnus FSH-Y2 and monoraphidium sp SS-B1 which are preserved in China General Microbiological Culture Collection Center on September 11, 2012 and April 15, 2013 by preservation numbers of CGMCC No.6551 and CGMCC No.7479, respectively. By virtue of the method disclosed by the invention, the solubility and the tolerance of the microalgae culture system to the high-concentration CO2 are improved, a carbon sequestration efficiency is improved and the yield of the microalgae oil is obviously improved, and the obtained microalgae oil can be used for producing biodiesel.