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Method for realizing grease accumulation of microalgae on basis of waste molasses-alcohol mash culture

A technology of molasses alcohol and waste mash, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of accumulation of oil, low biomass yield and high-quality yield, and achieve energy saving, oil Content and biomass increase, the effect of protecting the environment

Inactive Publication Date: 2018-11-06
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of adjunct biomass and high-quality yield is low, so adjunct is generally not suitable for accumulating oil

Method used

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  • Method for realizing grease accumulation of microalgae on basis of waste molasses-alcohol mash culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of medium: Gradiently dilute the waste mash 1600 times, as the Monoraphidium sp. FXY-10 basal medium, then add MgSO to the basal medium 4 ·7H 2 O: dosage 182.2mg / L; adjust the pH value to 6.8-7.0;

[0026] (2) Cultivation of oleaginous microalgae: sterilize under high pressure and high temperature for 20 minutes, insert Monopodella oleaginum, and control the algae cell density at 10 6 Melatonin was added to the culture medium so that the final melatonin concentration was 1 μmol / L, the light culture temperature was 25°C, the light intensity was 3500 lux, and the shaker speed was 150 r / min, and light shake flask culture was carried out.

[0027] (3) Preparation of biodiesel: enrich the microalgae cultured to the stable stage, centrifuge at 3500r / min for 10min, wash twice with distilled water and freeze-dry; add 2 times the weight of freeze-dried algae powder Quartz sand was ground for 20 minutes and oil was extracted with chloroform-methanol with a vol...

Embodiment 2

[0030] (1) Preparation of culture medium: the waste mash was diluted 1550 times, as the Monoraphidium sp. FXY-10 basal medium, then add MgSO to the basal medium 4 ·7H 2 O: dosage 176.5mg / L; adjust the pH value to 6.8-7.0;

[0031] (2) Cultivation of oleaginous microalgae: sterilize under high pressure and high temperature for 20 minutes, insert Monopodella oleaginum, and control the algae cell density at 1.2×10 6 cells / ml, add melatonin to the culture medium so that the final melatonin concentration is 10 -3 μmol / L, the light culture temperature is 23°C, the light intensity is 4000 lux, and the shaker speed is 130r / min, and light shake flask culture is carried out.

[0032] (3) Preparation of biodiesel: enrich the microalgae cultured to the stable stage, centrifuge at 3500r / min for 10min, wash twice with distilled water and freeze-dry; add 2 times the weight of freeze-dried algae powder Quartz sand was ground for 20 minutes and oil was extracted with chloroform-methanol w...

Embodiment 3

[0035] (1) Preparation of medium: Gradiently dilute the waste mash 1650 times, as the Monoraphidium sp. FXY-10 basal medium, then add MgSO to the basal medium 4 ·7H 2 O: dosage 187.5mg / L; adjust the pH value to 6.8-7.0;

[0036](2) Cultivation of oleaginous microalgae: sterilize under high pressure and high temperature for 20 minutes, then insert Monopodella oleaginum, and control the algae cell density at 1.5×10 6 cells / ml, add melatonin to the culture medium so that the final melatonin concentration is 10 -4 μmol / L, light culture temperature is 26°C, light intensity is 2500 lux, shaker speed is 160r / min, and light shake flask culture is carried out.

[0037] (3) Preparation of biodiesel: enrich the microalgae cultured to the stable stage, centrifuge at 3500r / min for 10min, wash twice with distilled water and freeze-dry; add 2 times the weight of freeze-dried algae powder Quartz sand was ground for 20 minutes and oil was extracted with chloroform-methanol with a volume r...

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Abstract

The invention discloses a method for realizing grease accumulation of microalgae on the basis of waste molasses-alcohol mash culture, and belongs to the technical field of biodiesel. The method comprises the following steps: waste molasses mash is diluted to serve as a minimal medium for Monoraphidium, then MgSO4.7H2O is added to the minimal medium, pH is regulated to be 6.8-7.1, and a medium is prepared; the medium is sterilized under high temperature and high pressure and inoculated with oil-producing Monoraphidium, density of algae cells is 1*10<6>-1.5*10<6> / ml, then melatonin mother liquorprepared from absolute ethyl alcohol is added to diluted induction algae liquid, the final melatonin concentration is enabled to be 10<-4> mu mol / L-1 mu mol / L, and the algae liquid is cultured underintense illumination; microalgae cultured to a stable stage are enriched, grease is extracted, and biodiesel is prepared. The method is simple and feasible, culture time of the oil-producing Monoraphidium can be shortened substantially, production cost is reduced, and grease yield is increased remarkably, so that efficiency for producing the biodiesel from the microalgae is improved greatly.

Description

technical field [0001] The invention relates to a method for cultivating oil accumulation of microalgae with molasses alcohol waste mash, which belongs to the technical field of biodiesel. Background technique [0002] Biodiesel refers to a renewable diesel fuel that can replace petrochemical diesel and is made from vegetable oil, microbial oil, animal oil, waste cooking oil, etc. through transesterification or thermochemical processes. Compared with conventional diesel, it has the characteristics of environmental protection, renewable, easy to degrade, low pollutant emission after combustion, and low greenhouse gas emission. [0003] Microalgae are used as raw materials to replace terrestrial plants to produce biofuels, because they can perform photosynthesis and can effectively use solar energy to convert water, CO 2 and inorganic salts into organic matter. Microalgae have the advantages of high photosynthetic utilization rate, fast growth rate, low requirement for growt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/649Y02E50/10
Inventor 余旭亚宋雪婷董训赞
Owner KUNMING UNIV OF SCI & TECH
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