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Method for cultivating monoraphidium sp. to produce biodiesel by using walnut shell extracting solution

The technology of single needle algae and walnut shell is applied in the field of biodiesel, which can solve the problems of complex extraction process, long cultivation time, low oil yield and the like, achieves simple and easy method, increases utilization efficiency, and improves single needle algae oil production. rate effect

Inactive Publication Date: 2019-06-25
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complex extraction process of substances in walnut shells, most of the walnut shells are directly burned or discarded directly with the sewage pipes, and resources are wasted.
[0005] In the prior art, the method of cultivating Monopodella to produce oil by using walnut shell extract has a long culture time and low oil yield

Method used

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  • Method for cultivating monoraphidium sp. to produce biodiesel by using walnut shell extracting solution
  • Method for cultivating monoraphidium sp. to produce biodiesel by using walnut shell extracting solution
  • Method for cultivating monoraphidium sp. to produce biodiesel by using walnut shell extracting solution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: a kind of method that utilizes walnut shell extract to cultivate monopodella to produce biodiesel, the specific steps are:

[0037] (1) Add water to the walnut shell and boil for more than 4 hours until the mass ratio of the liquid in the system to the walnut shell is 8:1, separate the solid from the liquid, and use the liquid as the basal medium, add glucose to the basal medium, and use sodium hydroxide The solution adjusts the pH value of the culture medium to 6.82, and then sterilizes to obtain the monospora culture medium; wherein the solid-liquid ratio g:L of glucose to the basic culture medium is 1:1;

[0038] (2) Inoculate Monospiria into the culture medium of Monospiria in step (1), and then cultivate it under the condition of temperature of 25°C and light intensity of 6800 lux; wherein Monospiria is Monospiria Monoraphidium For sp.QLZ-3, the inoculum amount of Monospinatus is 0.4g / L;

[0039] (3) Determination of the biomass of Monopodella under ...

Embodiment 2

[0046] Embodiment 2: a kind of method that utilizes walnut shell extract to cultivate monopodella to produce biodiesel, the specific steps are:

[0047] (1) Add water to the walnut shell and boil for more than 4 hours until the mass ratio of the liquid in the system to the walnut shell is 8:1, separate the solid from the liquid, and use the liquid as the basal medium, add glucose to the basal medium, and use sodium hydroxide The solution adjusts the pH value of the culture medium to 7.00, and then sterilizes to obtain the monospora culture medium; wherein the solid-liquid ratio g:L of glucose to the basic culture medium is 2:1;

[0048] (2) Inoculate Monospiria into the culture medium of Monospiria in step (1), and then culture it under the condition of temperature of 24°C and light intensity of 6900 lux; wherein Monospiria is Monospiria Monoraphidium For sp.QLZ-3, the inoculum amount of Monospinatus is 0.4g / L;

[0049] (3) Determination of the biomass of Monopodella under th...

Embodiment 3

[0056] Embodiment 3: a kind of method that utilizes walnut shell extract to cultivate monopodella to produce biodiesel, the specific steps are:

[0057] (1) Add water to the walnut shell and boil for more than 4 hours until the mass ratio of the liquid in the system to the walnut shell is 24:1, separate the solid from the liquid, and use the liquid as the basal medium, add glucose to the basal medium, and use sodium hydroxide The solution adjusts the pH value of the culture medium to 6.96, and then sterilizes to obtain the monostipium culture medium; wherein the solid-liquid ratio g:L of glucose to the base medium is 3:1;

[0058] (2) Inoculate Monospiria into the culture medium of Monospiria in step (1), and then culture it under the condition of temperature of 26°C and light intensity of 6800 lux; wherein Monospiria is Monospiria Monoraphidium For sp.QLZ-3, the inoculum amount of Monospinatus is 0.4g / L;

[0059] (3) Determination of the biomass of Monospina under the cultur...

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Abstract

The invention relates to a method for cultivating monoraphidium sp. to produce biodiesel by using a walnut shell extracting solution, and belongs to the technical field of biodiesel. The method includes adding water in a walnut shell, boiling for more than 4h until the mass ratio of the liquid to the walnut shell in the system (g:g) is (4-32):1, conducting solid-liquid separation, utilizing the liquid as a basic medium, adding glucose in the basic medium, adjusting the pH of the basic medium to 6.8-7.0 with a sodium hydroxide solution and then conducting sterilization to obtain a monoraphidiumsp. medium; inoculating the monoraphidium sp. into a monoraphidium sp. medium and culturing at the temperature of 24-26 DEG C and the illumination intensity of 6000-7000 lux; measuring the biomass ofthe monoraphidium sp. under the culture conditions and utilizing an organic solvent to extract grease in monoraphidium sp. cells after the growth of the monoraphidium sp. for 60-80 hours; adding sulfuric acid-methanol into the grease for methyl esterification for 3-3.5 hours to obtain the biodiesel. According to the method, the walnut shell extracting solution is used as the basic medium, the glucose is added for concurrent culture of the monoraphidium sp., and the grease yield of the monoraphidium sp. is improved.

Description

technical field [0001] The invention relates to a method for producing biodiesel by using walnut shell extract to cultivate Monopodella and belongs to the technical field of biodiesel. Background technique [0002] In recent years, due to the extensive use of fossil fuels, leading to the depletion of fossil fuels, biodiesel, as an environmentally friendly and renewable energy alternative to fossil fuels, has received widespread attention. Biodiesel refers to a renewable biomass fuel made from vegetable oil, animal oil, microbial oil, waste cooking oil, etc. through transesterification process. Compared with conventional diesel, it is renewable, easy to degrade, CO 2 Low emissions, high cetane number and other characteristics. [0003] As a raw material for biodiesel production, Monostipella has the advantages of easy cultivation, short growth cycle, high oil content, simple growth conditions, non-toxicity, and does not occupy arable land. However, due to the limitation of...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
CPCY02E50/10
Inventor 余旭亚乔腾生董训赞
Owner KUNMING UNIV OF SCI & TECH
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