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Method using molasses, alcohol, and waste undecanted wine to culture monoraphidium to produce diesel oil

A technology of biodiesel and molasses alcohol, applied in biofuel, fermentation and other directions, can solve the problems of poor economic operation, limited use range, insignificant effect, etc., and achieve the effect of shortening the growth cycle, improving the utilization rate and shortening the growth cycle.

Inactive Publication Date: 2015-11-11
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some technologies and methods currently used in China, such as oxidation ponds, agricultural irrigation methods, anaerobic biogas production, evaporation concentration, production of single-cell protein feed, etc., some of them have too high investment, poor economic operation, and insignificant effects. Harsh, limited scope of use, difficult to further promote the use of

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The waste mash was diluted 80 times in a gradient as the basic medium of oleaginous microalgae, and then the following inorganic salts and dosage (mg / L) were added to the basic medium: NaNO 3 :700mg / L; K 2 HPO 4 .3H 2 O:40mg / L; MgSO 4 .7H 2 O:75mg / L; CaCl 2 .2H 2 O: 40mg / L; Na 2 CO 3 :20mg / L; MnCl 2 .4H 2 O: 1mg / L; ZnSO 4 .7H 2 O:0.2mg / L; CuSO 4 :0.075mg / L; (NH 4 ) 6 Mo 7 o 24 : 4mg / L; Co(NO 3 ) 2 .6H 2 O: 0.04mg / L; Na 2 .EDTA: 1.25mg / L; H 3 BO 3 : 4mg / L; citric acid: 6mg / L; ferric citrate: 7mg / L; use 1.0M NaOH to adjust the pH value to 6.8; autoclave for 20min, insert Monopodella oleaginum, and control the algae cell density at 10 6 cells / mL, light culture temperature was 23°C, light intensity was 2500 lux, shaker speed was 130 r / min, and light shake flask culture was carried out.

Embodiment 2

[0025] Dilute the waste mash 90 times in a gradient, as the basic medium of oil-producing microalgae, and then add the following inorganic salts and dosages to the basic medium: NaNO 3 :600mg / L; K 2 HPO 4 .3H 2 O:50mg / L; MgSO 4 .7H 2 O:65mg / L; CaCl 2 .2H 2 O: 45mg / L; Na 2 CO 3 : 10mg / L; MnCl 2 .4H 2 O:2.5mg / L; ZnSO 4 .7H 2 O:0.1mg / L; CuSO 4 :0.1mg / L; (NH 4 ) 6 Mo 7 o 24 : 2mg / L; Co(NO 3 ) 2 .6H 2 O: 0.06mg / L; Na 2 .EDTA:0.5mg / L;H 3 BO 3 : 6mg / L; Citric acid: 4mg / L; Ferric citrate: 8mg / L, adjust the pH value to be 6.9. Sterilize under high pressure and high temperature for 20 minutes, insert Monopodella oleaginum, and control the algae cell density at 10 6 cells / mL, light culture temperature was 24°C, light intensity was 3000 lux, shaker speed was 140r / min, and light shake flask culture was carried out.

Embodiment 3

[0027] Dilute the waste mash 100 times in a gradient to serve as the basal medium for oleaginous microalgae, and then add the following inorganic salts and dosages to the basal medium: NaNO 3 :800mg / L; K 2 HPO 4 .3H 2 O: 30mg / L; MgSO 4 .7H 2 O:85mg / L; CaCl 2 .2H 2 O: 30mg / L; Na 2 CO 3 :30mg / L; MnCl 2 .4H 2 O:1.0mg / L; ZnSO 4 .7H 2 O:0.3mg / L; CuSO 4 :0.05mg / L; (NH 4 ) 6 Mo 7 o 24 : 2mg / L; Co(NO 3 ) 2 .6H 2 O: 0.02mg / L; Na 2 .EDTA: 2mg / L; H 3 BO 3 : 2mg / L; citric acid: 8mg / L; ferric citrate: 4mg / L; adjust the pH value to 7; sterilize at high pressure and high temperature for 20min, insert Monopodella oleaginum, and control the algae cell density at 10 6 cells / mL, light culture temperature is 25°C, light intensity is 3500 lux, shaker speed is 150r / min, and light shake flask culture is carried out.

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Abstract

The invention discloses a method using molasses, alcohol, and waste undecanted wine to culture monoraphidium to produce diesel oil. The method comprises the following steps: diluting waste undecanted wine, taking the diluted waste undecanted wine as the basic culture medium for oil-producing monoraphidium; adding inorganic salts into the basic culture medium, adjusting the pH value by NaOH to produce a culture medium; adding 250 mL of the obtained culture medium into a conical flask (500 mL), sterilizing, adding oil-producing monoraphidium, culturing monoraphidium; collecting the cells by a centrifugal separation method, extracting the grease by a chloroform / methanol extraction liquid, and finally adding 2 mL of sulfuric acid-methanol solution (3%) into the extract to carry out methyl esterification reactions so as to obtain the biodiesel oil. The provided method is simple and practical, is capable of largely reducing the culture time of oil-producing monoraphidium and production cost, and obviously increasing the grease yield, and thus the production efficiency of biodiesel oil is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of biodiesel, and in particular relates to a method for cultivating single needle algae to produce biodiesel with molasses alcohol waste mash. Background technique [0002] Biodiesel refers to methyl ester or ethyl ester fuel made from vegetable oil, animal oil, microbial oil, waste cooking oil, etc. through transesterification process. Compared with conventional diesel, it has the characteristics of renewable, easy to degrade, low pollutant emission after combustion, and low greenhouse gas emission. [0003] Microalgae have many advantages as raw materials for biodiesel production, such as simple cell structure; high photosynthetic efficiency, high biological output, fast growth and reproduction, and short growth cycle; and high oil content; low requirements for growth conditions, can be used in Survive under extreme conditions; and microalgae do not contain sulfur, non-toxic and degradable. Therefore, th...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCY02E50/10
Inventor 余旭亚丁柯尚敏敏车绕琼张和平赵永腾
Owner KUNMING UNIV OF SCI & TECH
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