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Method for producing starch by Monoraphidium

A single needle algae and starch technology, applied in the fields of biotechnology and bioenergy, can solve the problems of no discovery, single product, narrow application range, etc., and achieve the effects of improving starch yield, high starch content, and high starch content.

Active Publication Date: 2017-04-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But above-mentioned single needle algae is only used for producing grease, and product is single, and application range is narrow
No single needle algae and production method that can produce high starch have been found yet

Method used

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  • Method for producing starch by Monoraphidium
  • Method for producing starch by Monoraphidium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Pick the monospora on the plate ( Monoraphidium sp. ) SS-06 was inserted into BG11 medium, at a temperature of 30°C, with an aeration of 0.25vvm, CO 2 The content is 2v%, the stirring speed is 100rpm, the pH is 7.0, the light intensity is 7000lux, and the light-to-dark ratio is 14:10. The microalgae seed liquid is obtained by culturing to the logarithmic growth phase.

[0030] (2) Insert the microalgae seed solution into the BG11 medium rich in N element according to the inoculation amount of 5v%. The enrichment of N element means adding NaNO at 3.0g / L to the BG11 medium 3 , cultivate until the logarithmic growth phase stops, and discharge the supernatant after standing to settle to obtain algae cells. The culture conditions are the same as step (1).

[0031] (3) Insert the algae cells obtained in step (2) into the microalgae medium containing low N elements. The microalgae medium containing low N elements is to add 0.05 g / L NaNO to the BG11 medium 3 , and add ...

Embodiment 2

[0034] (1) Pick the monospora on the plate ( Monoraphidium sp. ) SS-06 was inserted into BG11 medium, at a temperature of 25°C, an aeration rate of 0.25vvm, CO 2 The content is 1v%, the stirring speed is 100rpm, the pH is 6.0, the light intensity is 5000lux, and the light-to-dark ratio is 14:10. The microalgae seed liquid is obtained by culturing to the logarithmic growth phase.

[0035] (2) Insert the microalgae seed solution into the BG11 medium rich in N element according to the inoculation amount of 5v%. The enrichment of N element means adding NH at 2.0g / L to the BG11 medium 4 NO 3 , cultivate until the logarithmic growth phase stops, and discharge the supernatant after standing to settle to obtain algae cells. The culture conditions are the same as step (1).

[0036] (3) Insert the algae cells obtained in step (2) into the microalgae medium containing low N elements. The microalgae medium containing low N elements refers to adding 0.1g / L NH to the BG11 medium 4 NO ...

Embodiment 3

[0039] (1) Pick the monospora on the plate ( Monoraphidium sp. ) SS-06 was inserted into BG11 medium, at a temperature of 30°C, with an aeration of 0.25vvm, CO 2 The content is 3v%, the stirring speed is 100rpm, the pH is 8.0, the light intensity is 8000lux, and the light-to-dark ratio is 14:10. The microalgae seed liquid is obtained from the cultivation until the logarithmic growth phase.

[0040] (2) Insert the microalgae seed liquid into the BG11 medium rich in N element according to the inoculation amount of 5v%. The enrichment of N element means adding NH at 2.5g / L to the BG11 medium 4 Cl, cultivate until the logarithmic growth phase stops, and discharge the supernatant after standing to settle to obtain algae cells. The culture conditions are the same as step (1).

[0041] (3) Insert the algae cells obtained in step (2) into the microalgae medium containing low N elements. The microalgae medium containing low N elements refers to adding 0.05g / L NH to the BG11 medium ...

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Abstract

The invention discloses a method for producing starch by Monoraphidium. The method comprises (1) preparing a microalgae seed liquid, wherein the microalgae is Monoraphidium sp. SS-06 and has a preservation number of CGMCC No. 10765, (2) inoculating an N element-rich microalgae culture medium with the Monoraphidium seed liquid, carrying out culture until a logarithmic growth phase, carrying out standing and sedimentation, discharging the supernatant and acquiring microalgae cells, (3) inoculating a low N element content microalgae culture medium with the microalgae cells, adding an ADP glucose pyrophosphatase inhibitor into the culture medium and carrying out continuous illumination culture until a stable period to obtain starch-rich microalgae cells. The method is conducive to proliferation of microalgae cells and accumulation of starch, can produce microalgae cells with high starch content and has the characteristics of simple processes, low cost and high yield.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for producing starch by using Monopodella algae. Background technique [0002] Due to the depletion of world oil resources and gradually rising prices, increasingly severe environmental protection issues, and a series of impacts caused by global climate change, since the 1970s, many countries have paid more and more attention to the development of bio-energy and bio-based chemicals. Among the many biomass energy and biomass chemicals, microalgae has received more and more attention due to its advantages of "not competing with others for food and land". [0003] Algae is one of the most primitive organisms with a wide distribution. Most algae live in water. They have a simple structure and have the characteristics of high photosynthetic efficiency, short growth cycle and fast speed. Algae include microalgae (single-celled eukaryotes), macroalgae (s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/89
Inventor 廖莎孙启梅王鹏翔师文静李晓姝樊亚超张霖高大成王领民乔凯
Owner CHINA PETROLEUM & CHEM CORP
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