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Process for preparing biodiesel from Monopodella cells

一种生物柴油、单针藻的技术，应用在单细胞藻类、基于微生物的方法、微生物等方向，能够解决成本高、生物柴油效率低等问题

Active Publication Date: 2020-08-21
HANGZHOU FUYANG GAOBO INFORMATION TECH SERVICE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the defects of low efficiency and high cost of preparing biodiesel from algae in the prior art, and provides a process for preparing biodiesel using Monopodella cells

Method used

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  • Process for preparing biodiesel from Monopodella cells
  • Process for preparing biodiesel from Monopodella cells

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The technique for preparing biodiesel by utilizing Monopodella cells comprises the steps of:

[0032] Streak inoculation of Cellulomonas xanthogenes on the slant medium to obtain a single colony; pick a single colony and inoculate it on the primary seed medium for cultivation, and then culture it on the secondary seed medium to obtain Cellulomonas xanthogenes Bacteria seed liquid; the components of the slant medium are: yeast extract 50g / L, glucose 30g / L, agar 20g / L; the components of the primary seed medium and the secondary seed medium are: corn flour 30g / L, glucose 20g / L, ammonium nitrate 1g / L, magnesium sulfate heptahydrate 0.2g / L, potassium dihydrogen phosphate 0.3g / L, dipotassium hydrogen phosphate 0.3g / L, ferrous sulfate heptahydrate 0.1g / L L;

[0033] Pick Monotipella, plant them in a container containing growth medium, light intensity 5000lux, light-dark cycle 24h, light-dark ratio 14:10, cultivate at 28°C, grow to the logarithmic growth phase, and get Monotip...

Embodiment 2

[0038] The technique for preparing biodiesel by utilizing Monopodella cells comprises the steps of:

[0039] Streak inoculation of Cellulomonas xanthogenes on the slant medium to obtain a single colony; pick a single colony and inoculate it on the primary seed medium for cultivation, and then culture it on the secondary seed medium to obtain Cellulomonas xanthogenes Bacteria seed liquid; the components of the slant medium are: yeast extract 50g / L, glucose 30g / L, agar 20g / L; the components of the primary seed medium and the secondary seed medium are: corn flour 30g / L, glucose 20g / L, ammonium nitrate 1g / L, magnesium sulfate heptahydrate 0.2g / L, potassium dihydrogen phosphate 0.3g / L, dipotassium hydrogen phosphate 0.3g / L, ferrous sulfate heptahydrate 0.1g / L L;

[0040] Pick Monotipella, plant them in a container containing growth medium, light intensity 5000lux, light-dark cycle 24h, light-dark ratio 14:10, cultivate at 28°C, grow to the logarithmic growth phase, and get Monotip...

Embodiment 3

[0052] The present invention has detected the influence of culture time on fatty acid components in oil:

[0053]The fatty acid composition of different algae species is very different, and the content of C16 and C18 is an important factor to determine the performance of oil. Taking Example 1 as an example, set the time gradient as 1, 3, 5, 7, 9 (d), and analyze the oil composition by GC-MS. The fatty acids produced by Monostipella during heterotrophic culture are mainly C16 and C18 . Such as figure 1 As shown, there is only a small amount of C16 in the early stage of culture, and as the culture time increases, the content of C16 increases, reaches the peak on the 5th day, and then decreases, but the decline is not large; C18 begins to be produced on the 3rd day , and then increased rapidly, and the increase was not obvious after the 7th day. Considering the cost and time of cultivation, it is most appropriate to choose to cultivate for about 7 days.

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Abstract

The invention belongs to the technical field of new bio-energy, and discloses a process for preparing biodiesel by using Monopodella cells. algae, step 2) oil extraction, step 3) biodiesel production. The biodiesel prepared by the process of the invention meets relevant standards, has high yield and low cost.

Description

technical field [0001] The invention belongs to the technical field of algae, and in particular relates to a process for preparing biodiesel by using Monopodella cells. Background technique [0002] As the amount of carbon dioxide in the atmosphere continues to increase, the greenhouse effect is also intensifying, and the burning of fossil energy accelerates this process. Therefore, finding new sustainable renewable energy can effectively reduce the emission of carbon dioxide into the atmosphere. Biodiesel is a long-chain fatty acid ester substance, which is a product obtained by reacting short-chain alcohols (methanol or ethanol) with certain fatty oils. The most commonly used production method for biodiesel is transesterification, that is, adding a certain amount of methanol to vegetable oil and heating it to a certain temperature. Under the action of a catalyst (acid or base), the fatty acid methyl ester is reacted and the by-product glycerin is separated. As a clean bi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/64C12R1/89C12R1/685C12R1/01
CPCC12P7/649C12P39/00C12P7/6463C12N1/20C12N1/14C12N1/12Y02E50/10
Inventor 宋庆恒潘宏涛陈生红洪元明李航
Owner HANGZHOU FUYANG GAOBO INFORMATION TECH SERVICE
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