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Culture method of rich-oil scenedesmus obliqnus

A technology of Scenedesmus obliquus and its cultivation method, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of unfavorable large-scale cultivation, collapse of the cultivation system, slow growth, etc., and achieve favorable flocculation recovery , expanding application conditions, and the effect of reasonable nutritional formula

Inactive Publication Date: 2017-12-08
HEILONGJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the main problems in the large-scale cultivation of Scenedesmus obliquus are slow growth, low biomass, and it is easily polluted by foreign species in the conventional culture system, which leads to the collapse of the culture system, which is not conducive to large-scale cultivation.

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  • Culture method of rich-oil scenedesmus obliqnus
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  • Culture method of rich-oil scenedesmus obliqnus

Examples

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Effect test

Embodiment 1

[0021] Example 1: Screening and community structure identification of Scenedesmus obliquus and mixed flora symbiotic system

[0022] The water sample extracted from the aeration tank of the wastewater treatment process of the biological flocculant production enterprise in Harbin City was packed into a sterile sampling bag and brought back to the laboratory. Microscopic observation found that there were many microorganisms and algae cells in the sample, and the corresponding microalgae and microalgae The associated flora was enriched, isolated and screened. Put the raw water samples into the simulated aeration biochemical tank reactor for continuous cultivation, place the reactor under natural light, mix with the pre-prepared simulated biological flocculant production wastewater, and aerate for a long time to keep the oxygen content at 4-5mg / L, cultured at 32°C, pH 7.5, COD maintained at around 800mg / L, TN around 90mg / L, TP around 20mg / L. The improved BG11 medium was used to s...

Embodiment 2

[0029] Example 2: Compound flora and activity verification

[0030] The composition ratio of flocculant producing bacterial community Rhizobium sp. (Rhizobium sp.), Bacillus (Bacillus sp.) and Klebsiella (Klebsiella sp.) in simulation example 1, and Scenedesmus obliquus ) for compounding. Wherein Rhizobium sp. selects specific species Rhizobium radiobacter (Rhizobium radiobacter), Bacillus sp. selects specific species as Bacillus sphaeicus, Klebsiella sp. The specific species selected is Klebsiella variicola. Among them, the single bacteria (algae) were activated with LB medium respectively, and the cell concentration was adjusted after activation, while the added concentration of Scenedesmus obliquus was two orders of magnitude higher than the concentration of the three flocculant-producing bacteria. The three flocculant-producing bacteria, namely Rhizobium ( Rhizobium sp.), Bacillus sp. and Klebsiella sp. were inoculated according to 4:4:3. All the above bacteria and alga...

Embodiment 3

[0032] Example 3: Optimization of Growth and Oil Content of Scenedesmus obliques with Different Nutrient Concentrations and Bioflocculant Addition Concentrations

[0033] Use sterile water to configure the optimized medium of Scenedesmus obliquus with the following formula: glucose 10000mg / L, NaNO 3 150mg / L, MgSO 4 ·7H 2 O 50mg / L, K 2 HPO 4 50mg / L, MnCl 2 4H 2 O 1.00mg / L, Co(NO 3 ) 2 ·6H 2 O0.35mg / L, H 3 BO 3 8.50mg / L, EDTA 30mg / L, FeSO 4 ·7H 2 O 2.50mg / L. Additions include Rhizobium sp. Rhizobium radiobacter, Bacillus sp. Bacillus sphaeicus and Klebsiella sp. Klebsiella Bacteria (Klebsiella variicola) mixed flora fermentation liquid 10mL, wherein the inoculation ratio of the three strains is 4:4:3. The specific composition and fermentation conditions of its mixed flocculant bioflocculant fermentation liquid are as follows: glucose 10g / ml, K 2 HPO 4 5g / L, MgSO 4 ·7H 2 O 0.2g / L, urea 0.5g / L, H 2 PO 4 2g / L, NaCl 0.1g / L, yeast extract 0.5g / L, pH 7.5. The ...

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Abstract

The invention provides a culture method with the advantages that the growth speed of the rich-oil scenedesmus obliqnus is improved; the cell density is increased; the culture time is shortened; the grease accumulation is promoted. Bioflocculant fermentation bacterium solution containing various composite flocculant-producing bacteria is added into a culture medium for culturing scenedesmus obliqnus. The method has the advantages that the scenedesmus obliqnus cells can fast grow; the cell density of the cultured scenedesmus obliqnus is great; particularly, due to the addition of the bioflocculant fermentation bacterium solution, the fast proliferation of the scenedesmus obliqnus cells can be promoted, so that the scenedesmus obliqnus cell concentration is increased; the culture time is shortened; the culture cost is further reduced; the grease content is obviously increased. The culture method provided by the invention is particularly suitable for large-batch scaled culture production.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and in particular relates to a method for cultivating Scenedesmus obliquus. Background technique [0002] With the shortage of resources in human society and the increasingly prominent environmental problems, the world is facing the crisis of energy shortage and ecological environment destruction. Therefore, seeking a green and renewable new energy has become a scientific issue that scientists from all over the world pay close attention to. Biodiesel is a clean, environment-friendly renewable energy, but due to its high cost of raw materials, the price of biodiesel is still higher than that of traditional diesel. Among the many biodiesel raw materials, microalgae has many advantages. Compared with terrestrial crops, microalgae has the characteristics of large biomass, short growth cycle, high lipid content, and does not occupy arable land. It has become a biofuel raw material. first c...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 李立欣战友宋志伟
Owner HEILONGJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
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