Scenedesmus sp. as well as culture method and application thereof
A technology of Scenedesmus and microalgae oil, which is applied in the fields of biotechnology and bioenergy, can solve the problems of the decline of microalgae's ability to produce oil, and achieve the effects of high added value, fast growth rate, and high total lipid content
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Embodiment 1
[0020] Scenedesmus HCS-02 obtained by isolation and domestication screening
[0021] (1) Obtaining the starting algae strain: In June 2011, 150mL of water samples were retrieved from the upper reaches of the Hunchun River in Jilin, filtered with gauze to remove large impurities, and 50ml of the filtered water samples were inoculated into 200mL of BG11 medium The enrichment culture was carried out in the medium, the light intensity of the culture was 5000Lux, the temperature was 25°C, the light-dark cycle was 24h, and the light-dark time ratio was 14:10. After about half a month of culture, the culture medium turned green. Dilute the water sample of the enrichment culture to 10 -5 times, spread it on the BG11 solid plate under sterile conditions and cultivate it. The light intensity of the culture is 5000Lux and the temperature is 25°C. After about 10 days of cultivation, green single algae appear on the plate, and the single algae is picked and dropped on the shaker flask. Cu...
Embodiment 2
[0026] Identification of strains
[0027] The DNA of HCS-02 algal cells was extracted by CTAB method, and the 18S rDNA gene was cloned, and the 3 positive clones obtained were sent to Shanghai Sangong Company for sequencing. The results of 18S rDNA gene sequencing analysis are shown in the sequence table. The 18S rDNA sequence was logged into the Genbank database for Blast comparison, and the results showed that it was consistent with Scenedesmus sp. There is the greatest similarity, its BLASTn value is 2610, the Max index value is 99%, it can be determined that HCS-02 is Scenedesmus ( Scenedesmus sp. ).
Embodiment 3
[0029] Application of Scenedesmus HCS-02
[0030] The algae liquid of HCS-02 in the logarithmic growth phase was inoculated in BG11 medium for cultivation. The formula of BG11 medium is shown in Table 2 and Table 3. The cultivation was carried out in a 500mL shake flask, and the OD of the culture solution after inoculation was 689.5 is 0.1. During the culture process, the light intensity was 5000 Lux, the culture temperature was 25°C, the light cycle was 24 hours, the light-dark time ratio was 14:10, the shake flask was shaken every 8 hours to make the sediment float up, and the culture time was 20 days in a stable period. At the end of the cultivation, the algae liquid was collected by centrifugation, and the starch content of the algae cells was determined by the amylase hydrolysis method after breaking the cell wall with enzyme / ultrasound. The biomass parameter of HCS-02 cultured for 20 days was measured to be 2.0337g / L, and the starch content of algal cells accounted for ...
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