430 results about "Melanocyte" patented technology

A non-invasive tool for skin disease diagnosis would be a useful clinical adjunct. The purpose of this study was to determine whether visible / near-infrared spectroscopy can be used to non-invasively characterize skin diseases. In-vivo visible- and near-infrared spectra (400-2500 nm) of skin neoplasms (actinic keratoses, basal cell carcinomata, banal common acquired melanocytic nevi, dysplastic melanocytic nevi, actinic lentigines and seborrheic keratoses) were collected by placing a fiber optic probe on the skin. Paired t-tests, repeated measures analysis of variance and linear discriminant analysis were used to determine whether significant spectral differences existed and whether spectra could be classified according to lesion type. Paired t-tests showed significant differences (p<0.05) between normal skin and skin lesions in several areas of the visible / near-infrared spectrum. In addition, significant differences were found between the lesion groups by analysis of variance. Linear discriminant analysis classified spectra from benign lesions compared to pre-malignant or malignant lesions with high accuracy. Visible / near-infrared spectroscopy is a promising non-invasive technique for the screening of skin diseases.

The invention discloses a whitening composition and a skin care product with the same. The whitening composition and the skin care product contain glycyrrhiza glabra extracts, arbutin and 3-o-ethyl ascorbic acid, and preferably contain nonapeptide-1, nicotinamide, ethylhexyl methoxycinnamate or composition of the nonapeptide-1, the nicotinamide and the ethylhexyl methoxycinnamate. Through mechanism analysis, the composition has the advantages that possibly due to the mutual action and influence among various ingredients, the generation of melanin is inhibited through inhibiting the activity of tyrosinase; the migration of melanocytes to the skin is retarded; in addition, the plant and microbial peptide sourced whitening ingredients and various antiallergic ingredients are selected to be combined to obtain the safe and mild whitening effect.

Methods for selection of compounds for treatment of obesity, compounds selected by the disclosed methods, and methods of treatment of obesity, wherein a selective melanocortin-4 receptor compound is identified, which compound is further characterized in that it attenuates the binding of both an agonist, including alpha-melanocyte stimulating hormone, and an inverse agonist, including agouti-related protein, to a melanocortin receptor, including melanocortin-4 receptor.

The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC), human induced pluripotent stem cells (hiPSC), somatic stem cells, cancer stem cells, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and / or hiPSC to nociceptors (i.e. nociceptor cells) using novel culture conditions. The nociceptors made using the methods of the present invention are further contemplated for various uses including, but limited to, use in in vitro drug discovery assays, pain research, and as a therapeutic to reverse disease of, or damage to, the peripheral nervous system (PNS). Further, compositions and methods are provided for producing melanocytes from human pluripotent stem cells for use in disease modeling.

The invention describes peptide analogues of a-melanocyte-stimulating hormone (a-MSH), which posses an increased efficacy compared to the native α-MSH peptide. The α-MSH analogues exhibit increased anti-inflammatory effects and increased capability to prevent ischemic conditions compared to α-MSH. The invention further discloses use of the peptides for the manufacture of pharmaceutical compositions for the treatment or prophylaxis of a condition in the tissue of one or more organs of a mammal, and moreover pharmaceutical compositions.

A method of inhibiting carcinogen mediated conversion of melanocyte cells and protecting DNA against carcinogen-mediated damage by administering a composition comprising a water insoluble carotenoid such as beta-carotene is disclosed.

There is a topical anti-aging composition which is configured to interfere with the production of tyrosinase, as well as melanin by reacting with melanocytes as well as reacting with, or acting on keratinocytes. The anti-aging composition comprises an emulsion comprising a plurality of active ingredients comprising a plurality of pigmentation reducing elements which are configured to interrupt or prevent the production of tyrosinase and melanin. In addition there are a plurality of antioxidants a plurality of anti wrinkling agents a plurality of anti-inflammatory agents at least one additional agent comprising at least one of an emollient, a proactant and a conditioning agent.

The invention discloses a compound traditional Chinese medicine extract and application thereof to a whitening, moisturizing and anti-aging skin care product. The compound traditional Chinese medicine extract is prepared from the following raw material medicines in parts by mass: 10-50 parts of selaginella, 10-50 parts of peach blossom, 10-50 parts of bletilla and 10-50 parts of pseudo-ginseng. The compound traditional Chinese medicine extract which is prepared from the raw materials has an effect of suppressing tyrosinase and proliferation of melanocyte, has a superior whitening effect, has an effect of proliferating and promoting fibroblast and has an excellent effect of promoting generation of ceramide. The whitening, moisturizing and anti-aging skin care product which is obtained by applying the compound traditional Chinese medicine extract to preparing the whitening, moisturizing and anti-aging skin care product has a durable natural whitening effect and can be used for improvingmetabolism and moisturizing effects of skin.

The invention relates to a method for cosmetically treating dark spots on the skin, wherein said method is intended for eliminating at least one of said dark spots located in a region of the hand, the face, the limbs or the chest area of a person suffering from such skin hyperpigmentation, characterised in that said method includes a step of applying a spray of cryogenic fluid to said region, which brings the skin temperature to a temperature of between −4° C. and −15° C. for a consecutive application period of from 2 to 10 seconds, in order to selectively act on the melanocytes, and to a device for carrying out said method.

This invention relates to monoclonal antibodies that recognize an antigen specific to melanocytic lesions. These antibodies are useful in methods of isolating melanoma cells and diagnosing melanocytic lesions. These antibodies are also useful for immunotherapy against melanoma.

Methods and formulations are provided to reduce pigmentation in skin, using an array of compounds selected from benzimidazoles, phenylthioureas, phenyltiols, phenylamines, bi- and multicyclic phenols, thiopheneamines, and benzothiamides. The compounds preferably inhibit pigment systhesis in melanocytes through the tyrosinase pathway. The methods can be used for lightening skin, and for treating uneven skin complexions which result from hyperpigmentation-related medical conditions such as melasma, age spots, freckles, ochronosis, and lentigo. The compounds can be used medically or cosmetically.

The invention discloses application of glacial water in preparation of an externally applied agent for the skin and a glacial water-containing externally applied agent for the skin. It is found for the first time that glacial water can obviously promote propagation of fibroblasts, improve synthesis of fibroblast collagen I and elastin laminin of the skin and substantially inhibit propagation activity of human epidermal melanocytes (HEM-m) and formation of melanin. Glacial water is used as one or more selected from the group consisting of an anti-aging active component, a whitening active component and a moisture-retaining component of the externally applied agent for the skin; so the glacial water-containing externally applied agent for the skin is natural and safe and is capable of improving skin elasticity, resisting crease, repairing impaired skin, reducing hyperpigmentation and whitening the skin.

The present invention is directed to methods for readily propagating somatic hair follicle stem cells or melanocyte stem cells. The methods comprise enhancing guanine nucleotide (GNP) biosynthesis, thereby expanding guanine nucleotide pools. This in turn conditionally suppresses asymmetric cell kinetics in the explanted cells. The methods of the invention include pharmacological methods and genetic methods. For example, the resulting cultured somatic hair follicle stem cells can be used for a variety of applications including cell replacement therapies such as hair transplants, gene therapies, and tissue engineering applications, such as the generation of artificial skin and skin regeneration strategies including skin grafts.

The invention belongs to the biotechnology filed and relates to a pigmented skin equivalent used for screening decolorizing agents and a constructing method thereof. A dermic skin equivalent layer is constructed by skin fibroblasts that are cultured in vitro and collagen, and the equivalent is constructed after inoculating keratinocyte and melanocyte on the skin equivalent layer by using gas-liquid plane three-dimensional culture technology. The obtained pigmented skin equivalent that is similar to the structure of skin can imitate the transportation process of melanin bodies, be used for screening the decolorizing agents and provide a more scientific model for the study of melanocyte in vitro biology and the screening of decolorizing agents and whitening cosmetics.

Methods and systems are described for a rapid and sustainable change in the pigment melanin content of melanocytes of the iris stroma, thereby to change the color of the eye. Also described are nanoparticle compositions for lightening the pigmented tissues or treating a pigmented tissue related disease.

A method for inducing melanogenesis in a human subject having a melanocortin 1 receptor (MC1R) variant allele associated with loss of or diminished receptor function comprises administering to said subject an amount of an α-melanocyte stimulating hormone (α-MSH) analogue effective to induce melanogenesis by the melanocytes in the skin or other epidermal tissue of the subject.

The invention discloses a composition with effects of whitening and removing freckles and a cosmetic. The composition with effects of whitening and removing freckles comprises the following components in parts by weight: 0.01-15 parts of a main whitening and freckle-removing component, 0.1-15 parts of a primary auxiliary whitening and freckle-removing component, 0.01-10 parts of a secondary auxiliary whitening and freckle-removing component and 0.1-8 parts of an assistant component, wherein the main whitening and freckle-removing component is a raw material capable of inhibiting the tyrosinase activity or changing eumelanin into light melanin; the primary auxiliary whitening and freckle-removing component is a raw material capable of inhibiting tyrosinase expression; the secondary auxiliary whitening and freckle-removing component is a raw material capable of increasing melanocyte-stimulating hormone receptor promoting activity of melanocyte; and the assistant component is a liquid crystal emulsifier. The composition with effects of whitening and removing freckles disclosed by the invention fully achieves the synergistic effects of various components, can achieve the effects of effectively improving dull skin and brightening the skin color and has obvious effects of whitening and removing freckles. Moreover, the irritation of the product can be effectively reduced, so that the product is safe.

The invention provides a stem cell exosome capable of repairing skin as well as a preparation method and application thereof. The preparation method comprises the following step: transducing decapeptide-12 into mesenchymal stem cell exosome, thereby obtaining the stem cell exosome capable of repairing skin. According to the stem cell exosome capable of repairing skin prepared by the method disclosed by the invention, the invention first discovers that, by binding the mesenchymal stem cell exosome and decapeptide-12 and by utilizing loading capacity and low immunogenicity of exosome cells, thedecapeptide-12 can reach melanocyte so as to further inhibit tyrosinase activity, the inflammatory response can be regulated, and skin related cell regeneration is promoted. The stem cell exosome capable of repairing skin prepared by the invention can achieve effects of eliminating erythema and edema when applied to sun-burned skin, can reduce melanogenesis and further reduce pigmentation and formation of black spots after sunburn, can be applied to the fields of cosmetics, medicines for treating skin diseases and the like, and has obvious effects and important application value.

A method of stimulating the production of melanin by the pigment-producing cells (keratinocytes and / or melanocytes) of the skin, in particular for the induction of skin tanning in humans, comprises administering alpha-MSH or an alpha-MSH analogue and exposing the skin to ultraviolet irradiation.

The present invention relates to a functional pigmented skin equivalent. The invention relates to an in vivo skin equivalent which is characterized by comprising at least one epidermis equivalent and at least one dermis equivalent. The in vivo skin equivalent further comprises melanocytes constitutively producing melanin and fibroblasts. The invention also relates to a method for preparing the skin equivalent.

Peptide antagonists of alpha-melanocyte stimulating hormone are disclosed, together with methods of inhibiting the effects of alpha-melanocyte stimulating hormone on cells or tissues sensitive to that hormone. In particular, methods for lightening the pigmentation of skin and for treating malignant melanoma, as well as kits for practicing the invention are also disclosed.

The invention discloses a tissue engineering material-based culture method and applications of melanophore. In the method of the invention, the tissue engineering technology is used to build carrier material with good biocompatibility and perform culture (co-culture) and transfer of melanophore or melanophore, fibroblast and keratinocyte and the method can be used in depigmentation diseases such as vitiligo and for the regulation of skin color. The invention relates to the preparation of the carrier material with biocompatibility and the building and transplanting of the cell-carrier composite material. The carrier material can be used to provide good carrier for cell culture, maintain cellular activity and solve the problem that the cell suspension is easy to lose in the cellular transplantation process; the inoculum density and culture time of cells can be regulated at the same time, the regulation to the skin colors of different individuals can be realized; and the carrier materialhas good performances such as damage resistance, tear resistance and easy transfer, has good function of promoting wound healing, and satisfies the use requirements of the large-area depigmentation disease and the regulation of skin color.

Embodiments of methods and apparatus use electron paramagnetic resonance spectroscopy to provide a signal from melanin to image a melanoma. Embodiments of methods and apparatus use electron paramagnetic resonance spectroscopy to provide a signal from melanin to detect metastatic melanoma in a sentinel lymph node. Embodiments of methods and apparatus use electron paramagnetic resonance spectroscopy to provide a signal from melanin to measure light penetration in melanocytes in skin.

Inhibiting the enzymic action of tyrosinase in the melanocyte prior to, during, or after ultraviolet (UV) radiation exposure, including over-exposure causing erythema, or sunburn, prevents the production of melanin and thereby melanoma. Melanoma can be prevented by using a tyrosinase inhibitor agent that inhibits the enzymic action of tyrosinase to prevent damage and / or death of melanocytes. The inhibitor agent can be applied to the skin or ingested.

The invention relates to a method for in-vitro directional differentiation inducing of a mesenchymal stem cell towards melanocyte, particularly relates to a method for in-vitro directional differentiation inducing of the mesenchymal stem cell with an umbilical cord source towards the melanocyte, and belongs to the technical field of biology. The mesenchymal stem cell with the umbilical cord source is cultivated by adopting an explants method, and then the mesenchymal stem cell with the umbilical cord source is identified, so as to determine the characteristics of the mesenchymal stem cell; a culture medium is induced to carry out in-vitro inducing differentiation to form the melanocyte by using improved melanine; and finally the cultivated melanocyte is identified. An ES cell source and a marrow or adipose tissue-derived stromal cell source are limited or not easy to obtain while the stem cell can be easily obtained from the umbilical cord, the immunogenicity is very low, and an individual difference is not easily generated, so that the method is suitable for large-scale clinical application, and the induced melanocyte can be applied to epichrosis leucasmus therapy.

A tissue-engineered artificial skin able to regulate the pigment secretion is configured through externally culturing the ceratin cells, fibroblasts and melanocyte of skin, proportionally mixing by tissue engineering method, and forming artificial skin.

The invention provides a mixed cultivation method of keratinocyte and melanocyte comprising: breaking the skin sample reserving epidermal layer and at least part of corium layer, cold digesting by pancreatin; collecting deposition organization; heat digesting by I type collagenase, collecting cells; primary inoculating, culturing in KGM-Gold medium; subcultring to the cells merge, culturing in KGM-Gold medium to the cells differentiate and stratify; collecting the formed skin sheet. The mixed cultivation method of keratinocyte and melanocyte uses the medium only for skin keratinocyte and melanocyte with which can avoid pollution of the other cells, and can get skin sheet including functional melanocyte and distributing uniformly. The mixed cultivation method of keratinocyte and melanocyte can get large area of skin sheet from quite small area of autologous sample, solve the problem of shortage of the skin source at skin grafting for the patient with large-area skin diseases, and apply in skin diseases of pigment deficiency such as leucoderma and so on.

The invention provides a marine bio-moisturizing and anti-aging cosmetic composition. The composition uses jellyfish extract and fucus extract as active ingredients and comprises 5 to 15% by weight ofjellyfish extract and 5 to 15% by weight of fucus extract. Through combination of jellyfish extract and fucus extract as active ingredients and other extract and a cosmetic matrix, the production offree radicals is reduced through supplementing antioxidant substances. Collagen or enzymes are supplied so that the DPPH free radicals are effectively eliminated, the tyrosinase activity is greatly inhibited, the biosynthesis of melanin in melanocytes is reduced and anti-aging effects are obtained. The marine bio-moisturizing and anti-aging cosmetic composition is natural and environmentally friendly and can well protect the skin.

The present invention relates to a composition for the improvement of skin conditions, comprising an extract of Vaccinium uliginosum as an active ingredient. The Vaccinium uliginosum extract has the effects of inhibiting and scavenging reactive oxygen species produced in skin tissue as a result of ultraviolet irradiation to the skin, effectively inhibiting tyrosinase activity to inhibit the production of melanin in melanin cells, inhibiting the secretion of cytokines in keratinocytes, promoting the production of procollagen, and inhibiting the decomposition of collagen. Thus, the extract will be useful for the prevention of skin photo aging caused by ultraviolet radiation, the enhancement of skin whitening and the improvement of wrinkles. In addition, the Vaccinium uliginosum extract is suitable to use as a component for improving skin wrinkles in cosmetics, foods and drugs, because the Vaccinium uliginosum extract can achieve the effect of improving skin conditions, even when it is applied to the skin or used internally.