96 results about "Collagen i" patented technology

Polymerized type I and / or III collagen based compositions for medical use as adhesives and sealants and preparation thereof are described. Prior to polymerization, the collagen monomers are prepared recombinantly whereby chemical modifications of the collagen are not needed to form such monomers. The type I and / or III collagen compositions are useful as medical adhesives for bonding soft tissues or in a sealant film for a variety of medical uses. In a further aspect of the present invention, the polymerized type I and / or III collagen composition includes agents which induce wound healing or provide for additional beneficial characteristics desired in a tissue adhesive and sealant.

A resorbable extracelluar matrix for reconstruction of cartilage tissue includes a mixture of collagen I and collagen II in a respective ratio of from about 1:19 to 19:1. The matrix can be utilized as a scaffold implant for vertebral or meniscal cartilage regeneration.

The invention discloses a tissue induction biomedical material, and particularly relates to a degradable active biological membrane / powder. The tissue induction biomedical material is prepared from, by weight, 0.1-99% of degradable medical polyurethane, 0-99.9% of polyhydroxyalkanoate, 0-99.9% of polylactic acid and 1-99.9% of collagen, wherein polyhydroxyalkanoate is one of PHA, PHB, PHBV, PHBHHx and P3HB4HB, polylactic acid is one of polyglycolic acid and polylactic acid-glycolic acid copolymer, and collagen is one or more of collagen I, collagen II, collagen VI, collagen IX, collagen X, collagen XI and other various in-vivo collagen. The ratio of all the components is adjusted according to the requirements of the implanting positions of different in-vivo tissue for material performance, degradation products and PH values, so better clinical service is provided.

The invention discloses a sgRNA specifically targeting human RSPO2 gene in a CRISPR-Cas9 system and an activation method and application thereof, and belongs to the field of biotechnology. The CRISPR-Cas9 system specifically activates the expression of the human RSPO2 gene, and introduces the activity of human hepatic stellate cells to activate Wnt signaling pathway so as to significantly upregulate the expression of liver fibrosis markers alpha-SMA and Collagen I. The CRISPR-Cas9 system designed for RSPO2 gene targets can effectively activate the hepatic stellate cells, thereby providing an effective way for liver fibrosis research.

The invention relates to a method for constructing a three-dimensional neural stem cell model in two steps by adopting the micro-fluidic technology. The method is characterized in that a rat tail collagen I is used as a three-dimensional support, a micro-pillar array type micro-fluidic chip is used as a culture platform, and a neural stem cell is cultured in two steps, wherein in the early culture stage, a culture medium for promoting the amplification of the neural stem cell is injected into a cell culture chamber, and in the later culture stage, a conditioned medium suitable for the growth of the neural stem cell and the daughter cells thereof is used, and a three-dimensional composite structure which is similar to a nerve tissue is formed by simulating the microenvironment of different neurogenesis stages in the body. The method provided by the invention is good in repeatability and can be used for construction a plurality of groups of samples. The adopted microfluidic culture system is in a microliter volume and can be regulated accurately, thus the amount of various high-cost cell growth factors, immunologic fluorescent antibodies and cell hormones used in the process of culturing the cell can be reduced greatly, and the cell culture cost can be lowered. The three-dimensional neural stem cell model is expected to be a nerve tissue substitute for screening a novel medicament or monitoring an environmental toxin.

The invention relates to a cartilage and bone repair composition comprising a group of human mesenchymal stem cells that are differentiated to the chondro-osteogenic lineage, by means of the amplification thereof with human serum and a transforming growth factor-beta 1 with a molecular domain for binding to collagen I (TGF-β1-DUC) obtained in eukaryotic expression systems, and a biocompatible material which is absorbed by the cells thus treated. The inventive composition can be employed using implants in the area to be repaired or it can be employed directly by injecting the cells in suspension either at the site of the injury or into the systemic circulation for the widespread distribution thereof.

A calcium phosphate bone cement setting to a calcium-deficient hydroxyl apatite is modified by an organic phosphate ester of orthophosphoric acid or a salt of an organic phosphate ester. The base cement contains preferably tricalcium phosphate, dicalcium phosphate (anhydrous), calcium carbonate and precipitated hydroxyl apatite. The organic phosphate ester is added to the base cement in an amount of 0.5 to 5 percent by weight. The bone cement can be modified further by adding mineralized collagen I.

The present invention relates to a cosmetic product with dispelling striae gravidarum effectiveness and a manufacturing method. Nutrient components synergy and can repair the elastic fiber effectively. Sweet almond oil and olive oil in the formula are rich in unsaturated fatty acid, which is totally identical with fats secretory of human; so essential fatty acid is added; the skin is repaired. Vitamin E has anti-aging and antioxidant effectiveness; small molecule faba bean peptide and nanometer parcel epidermal growth factor biomimetic peptide have strong physiological excitation signal which is easily absorbed by the skin; so the skin is stimulated to compose collagen which markedly amends the striae gravidarum. Centella asiatica extract can promote the composition of collagen I and III and can promote the composition of polysaccharide, which effective prevents and repairs the striae gravidarum.

Here the inventors provide a composition for topical application to the skin of animal comprising stearidonic acid in combination with one or octadecatrienoic acids (CODTAs). Preferably the composition comprises echium oil as a source of the stearidonic acid, and a plant lipid as a source of octadecatrienoic acid, such as borage oil (Borago officinalis), wheat germ oil (Triticum vulgare) rosehip oil (refined; Rosa mosqueta), jacaranda oil (Jacandra mimosi folia), and / or calendula oil (Calendula officinalis). The composition of the invention increases collagen I secretion and thus has particular utility for promoting collagen production in skin.

A laminate comprising a transparent collagen I type sheet and a human corneal endothelial cell culture layer provided on the sheet. An endothelial cell culture layer laminate usable in transplantation is provided.

The invention discloses application of glacial water in preparation of an externally applied agent for the skin and a glacial water-containing externally applied agent for the skin. It is found for the first time that glacial water can obviously promote propagation of fibroblasts, improve synthesis of fibroblast collagen I and elastin laminin of the skin and substantially inhibit propagation activity of human epidermal melanocytes (HEM-m) and formation of melanin. Glacial water is used as one or more selected from the group consisting of an anti-aging active component, a whitening active component and a moisture-retaining component of the externally applied agent for the skin; so the glacial water-containing externally applied agent for the skin is natural and safe and is capable of improving skin elasticity, resisting crease, repairing impaired skin, reducing hyperpigmentation and whitening the skin.

The present disclosure relates to bioactive biomatrix compositions. In one embodiment, the biomatrix composition is derived from human amniotic membrane. This biomatrix is ​​called HuBiogelTM. The HuBiogel™ composition precisely mimics the composition of naturally occurring basement membranes and is capable of supporting a variety of cell types in vitro and in vivo. The disclosed HuBiogel™ biomatrix contains laminin, collagen I, and collagen IV in one embodiment, and may further contain any combination of the following: nestin, tenascin, fibronectin, and proteoglycans . The biomatrix composition is substantially free of endogenous growth factors and proteolytic enzymes. The present invention also describes two-dimensional and three-dimensional culture systems and physiological / pathological model systems using HuBiogel™ compositions. HuBiogel(TM) compositions may be modified to contain desired growth stimulators, such as growth factors, polypeptides and small organic molecules, and may also contain growth inhibitory and / or therapeutic agents.

The invention discloses a preparation method of an in-situ-crossly-linked electrospun fibrous membrane dressing made from collangen I. According to the preparation method, the collagen I is dissolved in a novel environment-friendly solvent system (i.e. a composite system of [EMIM][Ac] and at least one lithium sal), so that the triple-helical structure of the collagen I is reserved to the maximum extent; natural cross-linking agent with good biocompatibility (alginate dialdehyde) is adopted to participate in the electrostatic spinning process of the collagen I, and crossly linked with the collagen I in situ. Secondly, through salt removal by simple water washing, freeze drying, sterilization and packaging, the in-situ-crossly-linked electrospun fibrous membrane dressing is obtained. The preparation method is simple and fast. Through the adoption of the preparation method, industrial production can be realized easily, and potential biotoxicity risks, brought by glutaraldehyde crosslinking agent, which is adopted universally at present, are effectively avoided. The in-situ-crossly-linked electrospun fibrous membrane dressing prepared according to the preparation method has the advantages of high biocompatibility, no cytotoxicity and high biological activity.

The peptides have the general following formula: X-Pro*-Pro*-Xaa-Y in which: —Xaa is selected from Leucine (Leu, L), Arginine (Arg, R), Lysine (Lys, K), Alanine (Ala, A), Serine (Ser, S), and Aspartic acid (Asp, D); —At the N terminal end of the peptide, X is selected from H, —CO—R1 and —SO2—R1; —At the C terminal end of the peptide, Y is selected from OH, OR1, NH2, NHR1 and NR1R2; —R1 and R2 are, independently from each other, selected from an alkyle, aryle, aralkyle, alkylaryl, alkoxy and aryloxy group, that can be linear, branched, cyclic, poly-cyclic, non-saturated, hydroxylated, carbonylated, phosphorylated and / or sulfured, and which skeletum can comprise an heteroatom, in particular an O, S and / or N atom;—Pro* correspond to a Proline, an analogue or derivative thereof; —if X is H then Y is selected from OR1, NH2, NHR1 and NR1R2, and if Y is OH then X is —CO— or —SO2—R1; and the peptide hypoxanthine-Pro-Pro-Arg being excluded. The invention provides the use of the peptides of above formula I to stimulate the synthesis of the molecules constituting the dermal extracellular matrix, including collagen I and IV and elastin. A cosmetic treatment according to the invention includes anti-aging, anti-wrinkles, improving mechanical properties of the skin, firmness / tone / elasticity / suppleness / flexibility, increasing density and volume of the skin, restructuring effect, fighting stretch marks, improving skin barrier and / or skin hydration.

A multi-well plate can be loaded with a range of compliant substrates. Commerically-available assays can be used to test cellular responses across a plate with shear modulus from 50 to 51200 Pascals. Cells can be grown in the plates, and can be manipulated and analyzed. Hydrogels can be attached to the bottom of a well. The plates can support the attachment and growth of different cell types and can be compatible with standard 96-well and 384-well plate assays. The mechanical properties of the hydrogels can be reproducible and stable to increase the shelf life of the substrate. The hydrogel can be compatible with growth of a variety of cell types, various attachment ligands such as collagen I, collagen IV, flbronectin, vitronectin, laminin, or RGD peptides and can be coupled to the gel surface.

The invention relates to a double-functional-layer oral restoration membrane and a preparation method thereof. The restoration membrane is formed by a loose porous layer and a dense antibacterial layer. The loose porous layer is formed by collagen I, oxidized polysaccharide and beta-tricalcium phosphate. The dense antibacterial layer is formed by compounding collagen I and oxidized chitosan. The restoration membrane has good mechanical performance, hydrophilia and biocompatibility, certain bacteria resistance and bacteriostasis performance and suitable and controllable degradation absorbability, preparation operation is easy, a good mechanical barrier effect is achieved, meanwhile, bonding of osteoblast and the membrane is facilitated, and Ca2+, P3- and the like are provided for formation of new teeth.

Fibrotic diseases are characterized by the replacement of healthy tissue with scar tissue and extracellular matrix in response to tissue damage. Here we describe the reduction of extracellular matrix (ECM) deposition, interstitial fibroblasts, interstitial volume, expression of Collagen I mRNA and protein, expression of profibrotic cytokines and macrophage infiltration by Cysteamine treatment.

The invention discloses a preparation method of a cell preparation for promoting the regeneration and the repair of traumas. The preparation method comprises the steps of shearing and digesting heel tendon tissues, carrying out centrifuging to remove supernate, adding an obtained tendon stem cell population into a culture solution, carrying out resuspension and filtering, inoculating the culture solution into a culture plate for culturing, mixing the culture solution with platelet rich plasma, and adding normal saline, so as to prepare a tendon stem cell population. Tendon stem cells are separated and cultured, tendon stem cells cultured in vitro are mixed with the platelet rich plasma to prepare the cell preparation, the platelet rich plasma contains a large number of growth factors such as PDGF, EGF, TGF-beta1 and IGF which have great promotion effects to the repair of tendons, the platelet rich plasma is capable of promoting the propagation of tendon cells and the expression of collagens I and III in vivo, and the tendon cells and the collagens I and III are key components of the tendons, so that an experimental result has a certain guiding effect to the repair of tendon injury of a human body.

The peptides have the general following formula: X-Pro*-Pro*-Xaa-Y in which: •Xaa is selected from Leucine (Leu, L), Arginine (Arg, R), Lysine (Lys, K), Alanine (Ala, A), Serine (Ser, S), and Aspartic acid (Asp, D); •At the N terminal end of the peptide, X is selected from H, —CO—R1 and —SO2—R1; •At the C terminal end of the peptide, Y is selected from OH, OR1, NH2, NHR1 and NR1R2; •R1 and R2 are, independently from each other, selected from an alkyle, aryle, aralkyle, alkylaryl, alkoxy and aryloxy group, that can be linear, branched, cyclic, poly-cyclic, non-saturated, hydroxylated, carbonylated, phosphorylated and / or sulfured, and which skeletum can comprise an heteroatom, in particular an O, S and / or N atom; •Pro* correspond to a Proline, an analogue or derivative thereof; •if X is H then Y is selected from OR1, NH2, NHR1 and NR1R2, and if Y is OH then X is —CO— or —SO2—R1; and the peptide hypoxanthine-Pro-Pro-Arg being excluded. The invention provides the use of the peptides of above formula I to stimulate the synthesis of the molecules constituting the dermal extracellular matrix, including collagen I and IV and elastin. A cosmetic treatment according to the invention includes anti-aging, anti-wrinkles, improving mechanical properties of the skin, firmness / tone / elasticity / suppleness / flexibility, increasing density and volume of the skin, restructuring effect, fighting stretch marks, improving skin barrier and / or skin hydration.

The invention provides a biological root scaffold. The biological root scaffold is prepared from animal-origin collagen I and hydroxyapatite, has certain pore structure in microscopic scale, has porosity 70%-95%, pore diameter 50-500 MuMu and has a macroscopically cylindrical shape, cylinder height is 9-12 mm; the shape of the outer side of a cylinder truncated by an axial plane perpendicular to the cylinder is circular or approximately circular; the scaffold is 11-14 mm in perimeter. The mineralized collagen-based biological root scaffold material and a biological root scaffold can be obtained by implementing the invention. The scaffold material has chemical composition and microscopic structure consistent with dentin of a natural tooth root, the shape of the scaffold is close to that of the natural tooth root, the micrometer porous structure and macroscopic internal structure of the scaffold help carry cells, growth factors, pharmaceuticals and the like for the ingrowth of the cells, and therefore the biological root scaffold has ingredients and structure consistent with those of the natural tooth root, is good for the construction of biological tooth roots in root regeneration application and has a promising clinical application prospect.

The present invention relates to use of hydrogel based on RGD-conjugated alginate with and without addition of nanocellulose and / or fibrin as a novel bioink for 3D Bioprinting of human skin, particularly dermis. RGD-conjugated alginate provides adhesion sites for the human fibroblasts which result in cell adhesion and stretching which contribute to upregulation of genes producing Collagen I. In this invention, RGD-conjugated alginate is used as one of the components of the bioink for 3D bioprinting. Another innovation described herewith is use of coaxial needle when 3D bioprinting with alginate and RGD-modified alginate bioinks. A coaxial needle makes it possible to crosslink the bioink upon 3D bioprinting operation and thus achieve high printing fidelity which is required for high cell viability, proliferation and production of extracellular matrix. In this invention, the novel RGD-modified alginate bioink together with human fibroblasts is 3D bioprinted and the resulting construct shows high cell viability, high cell proliferation, high degree of stretching of fibroblasts and high productivity of Collagen I. The cell bioink construct biofabricated with this invention is ideal for testing cosmetics and active ingredients of skin care products particularly those used for skin regeneration. It is also ideal to be used as skin grafts for skin repair for patients with damaged or burned skin.

A topical skin care lotion includes materials for supplementing the production of collagen I and III includes a base of lipophillic or lipophobic material and at least one amino acid. The amino acids are alanine and glycine. Other components of the lotion include iron, vitamin C, manganese, silica and cherry fruit extract. Together, these components assist the body with the production of collagen and promote healing of damaged skin. Antioxidants are also provided to protect the skin from oxidative stress by ultraviolet sunlight and environmental hazards.

Differentiation and stability of neural stem cells can be enhanced by in vitro or in vivo culturing with one or more extracellular matrix (ECM) compositions, such as collagen I, IV, laminin and / or a heparan sulfate proteoglycan. In one aspect of the invention, adult mammalian enteric neuronal progenitor cells can be induced to differentiate on various substrates derived from components or combinations of neural ECM compositions. Collagen I and IV supported neuronal differentiation and extensive glial differentiation individually and in combination. Addition of laminin or heparan sulfate to collagen substrates unexpectedly improved neuronal differentiation, increasing neuron number, branching of neuronal processes, and initiation of neuronal network formation. In another aspect, neuronal subtype differentiation was affected by varying ECM compositions in hydrogels overlaid on intestinal smooth muscle sheets. The matrix compositions of the present invention can be used to tissue engineer transplantable innervated GI smooth muscle constructs to remedy aganglionic disorders.

The invention discloses construction and application for tissue-engineered bone with BMSCs transfected with genes in a combining manner. According to the invention, recombinant lentiviral vectors carrying human bFGF, BMP-2 and GFP genes are constructed, BMSCs are transfected with the genes, the osteogenesis function of BMSCs can be enhanced through human bFGF and BMP2 gene combined-transfection, the fact that bFGF / BMP2 can adjust OPN, collagen-I and other osteogenesis nonspecific gene expression mechanisms is proved, and a GFP-marked tissue-engineered bone is constructed by gene transfected BMSCs composite heterologous bone scaffold material, and a jaw defect animal model is replanted. According to the invention, defects of single gene transfection are overcome, blood vessel forming and osteogenesis functions for solving difficulties in tissue-engineered bone tissue construction and application thereof to jaw defect repairing are obtained, a bone grafting material which has a wide application prospect is provided for mouth rehabilitation and bone transplantation treatment, and a high practical value and practical significance are realized.

In one aspect, the present invention provides methods for determining susceptibility to bone damage in a subject. In some embodiments, the methods comprise screening for polymorphisms in the MTHFR and collagen Iα1 genes that are associated with susceptibility to bone damage. In some embodiments, the methods comprise screening for elevated levels of homocysteine in a subject, wherein elevated levels of homocysteine are associated with an increased risk of bone damage. The methods of the invention may be used in predicting the response of a patient to treatment. Also provided are methods for prevention or reducing the risk of bone damage in a subject.

The invention provides a cyclic peptide including three collinear amino acids, arginine-lysine-lysine (RKK), for binding to the integrin α2I domain. The peptide is the integrin α21 region combined with collagens I and IV and A potent inhibitor of laminin-1 interaction. The present invention also provides methods of using these peptides to block integrin function and inhibit cell migration.

Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing secreted products obtained from the culture medium of human embryonic germ (EG) cell derivatives, such as embryoid body-derived cells. Substrates include compounds such as collagen I, fibronectin, or superfibronectin, or extracellular matrix, typically human derived.