The invention discloses a method for separating and purifying
heparin sodium and
heparan sulfate from
heparin byproducts. The method comprises the steps of dissolving raw
heparin byproducts, adding
potassium acetate to precipitate and separate
heparin sodium, adding
potassium acetate to precipitate
heparin sodium after precipitating and dissolving
heparin sodium, collecting a precipitate, dissolving and then conducting
fractional precipitation repeatedly with
anhydrous alcohol to prepare the heparin
sodium with a higher purity, adding a benedict
reagent and a saturated
sodium hydroxide solution to supernate, centrifuging to obtain a solution containing the
heparan sulfate, removing
copper ions with a decoppering agent, adding
active carbon to absorb the precipitate, decolorizing and filtering, and then absorbing and eluting with anion exchange resin to obtain the high-purity
heparan sulfate by separation. According to the method, the high-purity heparin sodium and heparan
sulfate can be separated again from the byproducts used for producing the heparin sodium, so that financial resources and
material resources required for storing or discarding a large number of heparin byproducts can be saved, wastes can be changed into valuables, the byproducts are recycled, the cost is saved greatly, and a value is created.