116 results about "Collagen type" patented technology

Polymerized type I and/or III collagen based compositions for medical use as adhesives and sealants and preparation thereof are described. Prior to polymerization, the collagen monomers are prepared recombinantly whereby chemical modifications of the collagen are not needed to form such monomers. The type I and/or III collagen compositions are useful as medical adhesives for bonding soft tissues or in a sealant film for a variety of medical uses. In a further aspect of the present invention, the polymerized type I and/or III collagen composition includes agents which induce wound healing or provide for additional beneficial characteristics desired in a tissue adhesive and sealant.

Neural stem cells which can be provided stably and which are free from the problem of compatibility in transplantation are disclosed. The stem cells are separated from human amniotic mesenchymal cell layer and express vimentin, nestin and BrdU which are markers of neural stem cells. The stem cells can also be differentiated to cells expressing alkaline phosphatase, that is, osteocytes, and to cells expressing collagen type II, that is, chondrocytes.

An animal toy containing food having a first portion with food delivery means integrated therein adapted to securely house solid food treats, including a nutritional pet supplement, to be removed by the animal during play or through chewing action, and a second side with a plurality of gum stimulation teeth integrated within and projecting therefrom that act to massage the gums of the animal. Food delivery means may be provided as a plurality of cavities integrated within the first portion (and optionally, the second portion), preferably having a grooved or threaded interior for securely holding food pieces securely therein. Alternatively, the food delivery means is provided as a food portion composing the first portion. A pet supplement may be utilized, including joint preserving and joint rebuilding compositions comprising chicken collagen type II, glucosamine hydrochloride and chondroitin sulfate, a vitamin composition comprising vitamins C, D and K, a mineral composition comprising calcium, magnesium, zinc, copper, manganese and boron, a herbal anti-oxidant cofactor blend comprising citrus bioflavonoids, red grapes anthocyanins, turmeric rhizome, boswellia resin and fennel seed.

The present invention relates to compositions for the protection, treatment and repair of connective tissues in humans and animals comprising any or all of anabolic, anti-catabolic, anti-oxidant and analgesic agents, including aminosugars, S-adenosylmethionine, arachadonic acid, GAGs, including pentosan, collagen type II, tetracyclines or tetracycline-like compounds, diacerin, super oxide dismutase, L-ergothionine, one or more avocado/soybean unsaponifiables, and an analgesic, e.g., acetaminophen, and to methods of treating humans and animals by administration of these novel compositions to humans and animals in need thereof.

This invention discloses methods to attach and grow a monolayer of cultured human corneal endothelial cells onto the endothelial side of the stroma synthesized from biopolymer to generate a more bio-equivalent artificial cornea. The approaches will include the use of attachment and growth promoting agents such as fibronectin, laminin, RGDS, collagen type IV, bFGF conjugated with polycarbophil, and EGF conjugated with polycarbophil. The patent also describes a method to create a self-sustaining polymer containing adhesive molecules and growth factors to support the attachment and proliferation of cultured human corneal endothelial cells for corneal transplantation either as a half-thickness device or full-thickness button replacement. An approach for the implantation of cultured retinal pigment epithelial (RPE) cells into the sub-retinal space for treatment of age-related macular degeneration (ARMD) is disclosed in this invention. This method will enable the delivery of the transplanted RPE in a sheet of monolayer cells and will be better suited to perform their physiological function.

The invention discloses a beautifying and nursing essence and a preparation method thereof. The essence is prepared from an epidermis cell nutrient solution and contains a plurality of cell growth factors, amino acids and vitamins. The essence mainly comprises an epidermal growth factor, a fibroblast growth factor, collagen type I, a stem cell growth factor, a horn cell growth factor and amino acids and vitamins including gamma-aminobutyric acid, coenzyme Q, medical vitamin C, medical vitamin B5 and medical vitamin E. The preparation method for the essence comprises the following steps: acquisition of epidermis of umbilical cord tissue of a newborn; cell culture; collection of a cell nutrient solution; filtration sterilization; condensation of a volume; addition of active components; addition of glycerin and hyaluronic acid; etc. The beautifying and nursing essence provides a plurality of endogenous nutritional ingredients with physiological activity for the skin, can promote metabolism of epidermis cells and repair damaged cells, has a moisture retention effect and is applicable to skin beautifying and nursing.

This invention provides porated cartilage products, methods of producing porated cartilage products, and methods of treating subjects by administering cartilage products. Optionally, the cartilage products are sized, porated, and digested to provide a flexible cartilage product. Optionally, the cartilage products comprise viable chondrocytes, bioactive factors such as chondrogenic factors, and a collagen type II matrix. Optionally, the cartilage products are non-immunogenic.

The present invention relates to compositions for the modulation of inflammation in connective tissues in humans and animals and the modulation of markers of such inflammation, including COX-2, TNF-α, IL-1β, iNOS, p38, and chemokines, comprising any or all of anabolic, anti-catabolic, anti-oxidant and analgesic agents, including aminosugars, S-adenosylmethionine, arachadonic acid, GAGs, including pentosan, collagen type II, tetracyclines or tetracycline-like compounds, diacerin, super oxide dismutase, L-ergothioneine, methylsulfanylmethane, one or more avocado/soybean unsaponifiables, and an analgesic, e.g., acetaminophen, and to methods of treating humans and animals by administration of these novel compositions to humans and animals in need thereof.

The present invention relates to a solid composition useful for tissue gluing, tissue sealing and haemostasis consisting essentially of a) a carrier which has at least one of the following physical properties: elasticity module in the range of 5-100 N/cm, density of 1-10 mg/cm3, chamber diameter of more than 0.75 mm and less than 4 mm and/or having a chamber diameter average below 3 mm and evenly distributed and fixed upon said carrier, b) solid fibrinogen, and c) solid thrombin. The carrier is a biodegradable polymer such as a polyhyaluronic acid, polyhydroxy acid, e.g. lactic acid, glucolic acid, hydroxybutanoic acid, a cellulose, gelatine or collagen, such as a collagen sponge, e.g. a collagen sponge consisting essentially of collagen type I fibres. The fibrinogen and thrombin are preferably human, purified from a natural source, or transgenic or recombinant human fibrinogen and/or thrombin. In a preferred embodiment the composition does not comprise any antifibronolytic agent such as aprotinin, epsi-aminocaproic acid or alpha2-antiplasmin,

The present invention relates to a method for inducing the bionic calcification in collagen fibers through a polymer polyelectrolyte, and applications thereof. The method comprises: treating different types of collagen scaffolds in a calcium phosphate mineralized solution containing a cationic polyelectrolyte or anionic polyelectrolyte to obtain the bionic calcification collagen scaffold material having the inside-fiber mineralization characteristic, wherein the collagen scaffold is recombinant collagens type I having different sources, completely demineralized bone collagen, completely demineralized dentin collagen, or rat tail collagen, and the mineralization liquid is a calcium phosphate solution added with a cationic polyelectrolyte or anionic polyelectrolyte. According to the present invention, through the stabilizing effect of the polyelectrolyte on the calcium phosphate solution, the novel bionic calcified collagen scaffold material with characteristics of similar natural bone tissue structure, good mechanical property, good biocompatibility and suitable biodegradability is constructed.

The invention discloses a scar repair material which is prepared by the following method: dissolving 0.5-5 parts by weight of water-soluble chitosan or chitosan the deacetylation degree of which is 75-97% into 10-100 parts by weight of water or an acetic acid aqueous solution the mass concentration of which is 1-5% while stirring; adding 1-3 parts by weight of polyoxyethylene Sorbitan lauric acid monoester which has emulsifying action and 5-10 parts by weight of humectant glycerol to obtain a transparent gelatinous solution; adding 20-90 parts by weight of polydimethylsiloxane into the gelatinous solution under homogeneity; and evenly mixing to obtain the scar repair material. The scar repair material disclosed in the invention can actively and passively moisturize skin, has the efficacy on actively resisting and inhibiting bacteria, has the action on quickening the healing of skin wound surface, has better repair and protection actions on tiny flaws and infection on the scar surface as being used on the scar surface, and can inhibit the secretion of collagen type I in cheloid skin so as to lower the ratio of collagen type I/collagen type III.

The invention relates to a large-scale preparation method of fish scale type I collagen peptides. The preparation method comprises the following steps: firstly, performing alkali and acid pretreatment on marine fish scales or freshwater fish scales serving as raw materials to remove impurities, and directly performing directed enzymatic hydrolysis on collagen type I in the fish scales by adopting a one-step biological enzymatic hydrolysis technology to form collagen peptides type I with relatively centralized molecular weight distribution; secondly, separating, purifying and concentrating directed enzyme liquid at the normal temperature by adopting a continuous centrifugal separation and membrane separation combined technology to obtain a fish scale type I collagen peptide solution with purity of not less than 95% and molecular weight of less than 1,000 Dalton; finally, quickly drying finished fish scale type I collagen peptides by adopting a spray-drying technology. The method is simple and feasible in overall process, efficient, energy-saving, short in production cycle and suitable for large-scale production; a fish scale type I collagen peptide product produced by adopting the method is high in purity and good in quality, and the content of peptides with molecular weight of less than 1,000 Dalton is more than 97%.

An articular cartilage graft and a preparation method thereof are provided. The prepared articular cartilage graft is composed of a superficial layer, a middle layer and a deep layer from outside to inside; the thickness, shape and size of each layer are all matched with a cartilage injury part, and thus preoperative shaping is not required; after grafting, the articular cartilage graft has layer distribution corresponding to distribution of each layer of surrounding normal cartilages, is conducive to intercellular signal transmission, transduction and regulation, and can be better integrated with surrounding normal cartilage tissues; the articular cartilage graft has structure characteristics consistent with those of the natural cartilages, the collagen type II content is gradually decreased from the superficial layer to the deep layer, the GAG content is increased gradually from the superficial layer to the deep layer, and compression resistance and wear resistance are good; and chondrocytes in the cartilage graft are wrapped with an extracellular matrix, have low immunogenicity, allow generation of immunologic rejection to be avoided after grafting, can survive for a long term and exert functions, and improve cartilage repair long-term curative effects.

A device for repairing a dural puncture, including: a patch formed of collagen type of material that includes a bulbous head portion that is larger than the puncture opening, and a neck portion that is proportioned to fit through the puncture opening.

This invention provides porated cartilage products and methods of producing porated cartilage products. Optionally, the cartilage products are sized, porated, and digested to provide a flexible cartilage product. Optionally, the cartilage products comprise viable chondrocytes, bioactive factors such as chondrogenic factors, and a collagen type II matrix. Optionally, the cartilage products are non-immunogenic.

The invention discloses a method for producing human collagen type II, and belongs to the technical field of gene engineering. The method is characterized in that a baculovirus multi-gene expression system is adopted, insect cells are infected by the bacteria converting the baculovirus multi-gene expression system to produce baculovirus with multiple genes expressed, the virus liquid is infected in the insect cells or injected into silkworm larva bodies to produce recombinant human collagen type II full-length protein, the protein structure is that 3 peptide chains with (G-X-Y) n repetitive sequences form a three-strand helical structure, to both keep the conformation of the natural collagen type II and have the activity of the natural collagen type II. By adopting the method disclosed by the invention, multiple genes can be expressed to facilitate the production of then collagen type II full-length protein; the method disclosed by the invention is simple in process method, simple and convenient to operate and short in process route.

The invention discloses a method for extracting pure type II collagen from chicken cartilage. The method is characterized by including, using the fresh chicken cartilage as raw material, removing other non-cartilage components, performing degreasing pretreatment, using mixed solution of 4mol/L NaCl and 0.05mol/L Tris-HCl to remove soluble impure protein, using mixed solution (pH 7.5) of 4mol/L guanidine hydrochloride and 0.05mol/L Tris-HCl to remove insoluble impure protein, using acetic acid solution containing 1-2% pepsin for solid matter enzymolysis, then centrifuging to take supernate, adding NaCl until final concentration is 3-4.2mol/L, 1.2-1.8mol/L and 0.8-1.0mol/L, successively salting out to remove other types of collagen, repeatedly adding NaCl until the final concentration is 0.8-1.0mol/L, salting out for 2-5 times, and dialyzing to obtain the pure type II collagen. The type II collagen produced by the method is single in type, high in bioactivity, stable in physical and chemical property. Meanwhile, the method is simple to operate and capable of benefiting large-scale production.

The present invention provides a method for preparing a biomaterial involving extracting Collagen Type I (Col I) protein from small intestine submucosa (SIS) under conditions that preserve the native helical configuration. The extract of the present method contains a substantial amount of the alpha helical form of Col I. Further the present method includes inducing the Col I protein to form a polymer material in solution or on a target surface.