The invention relates to a
cell-free compound
artificial skin and a preparation method thereof. The compound
skin of the invention is provided with a double layer structure of a
dermis layer and an epidermis layer; the
dermis layer is a loose porous structure made of
extracellular matrix; gel-shaped epidermis-like materials are coated on the upper surface of the
dermis layer to solidify, thus forming the epidermis layer, and the epidermis layer is inserted into a void structure of the surface of the dermis layer; compared with the existing
skin substitutes, the
skin of the invention has the functions of promoting the regeneration of
wound skin, enhancing the elasticity, flexibility and
mechanical wear resistance of the skin after the wound is healed, reducing the scar proliferation, controlling the
contracture, improving the success rate of skin implantation and improving the healing quality; the skin of the invention has a sustained release function and can make individual-based treatment for different diseases by releasing different drugs and being directly applied to the treatment of skin defects caused by inflammations, ulcers, burn wounds and iatrogenic causes; the epidermis layer and the dermis layer are embedded together organically with a tight structure; the shapes, sizes and the thicknesses of the skin can be prepared according to specific requirements. The skin of the invention has wide sources of preparation materials, simple production process, short production period, long period of product storage and convenient transportation.