The invention relates to a medical biological dressing, and in particular relates to a medical biological wrinkle-removing and speckle-inhibiting dressing and a preparation method thereof. The dressing comprises hyaluronic acid, olive oil, collagen, geranium essential oil, camellia oil, apricot kernel oil, active growth factors, pearl powder, vitamin A, vitamin C, vitamin E, amino acid, garlic oil and mint oil. By adopting the formula, skin regeneration organs are reserved and activated to the most extent; the skin condition is improved; cell regeneration is promoted; the yield of skin collagen is increased; therefore, skin whitening, speckle removing and wrinkle smoothing are promoted; a good foundation is laid for repairing wounds; and the medical biological wrinkle-removing and speckle-inhibiting dressing has the excellent effects in early treatment of skin wrinkle, striae gravidarum, crows feet, stain and scar.