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Nano silver-porcine acellular dermal matrix (PADM) biological dressing and preparation method thereof

A technology of decellularized dermis and biological dressings, applied in the field of medical devices, can solve the problems of complex chitosan membrane processing, lack of bactericidal ability, frequent dressing changes, etc., achieve durable anti-infection ability, reduce the number of dressing changes, The effect of good biocompatibility and stability

Inactive Publication Date: 2011-04-20
NANTONG UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The structure of pigskin is similar to that of human skin, and the anatomical and physiological characteristics of the skin are basically the same, and the source is wide and the cost is low. Traditional biological dressings such as allogeneic skin and heterogeneous pigskin have played a very good role in the treatment of second-degree burn wounds. However, there are also disadvantages such as easy infection of the wound surface, frequent dressing changes, and the risk of cross-infection.
And various synthetic dressings themselves do not have the ability to kill bacteria
This limits its wide application in clinical practice.
[0004] Also have the research (CN 1775300A) to nano-silver bionic dressing, but it is comparatively complicated to the processing of the chitosan membrane as substrate, it is also relatively difficult to obtain uniform membrane body in the processing process, simultaneously it is membrane structure, not Facilitate the growth of fibroblasts and vascular endothelial cells into the dermis in the proper histological manner

Method used

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  • Nano silver-porcine acellular dermal matrix (PADM) biological dressing and preparation method thereof
  • Nano silver-porcine acellular dermal matrix (PADM) biological dressing and preparation method thereof
  • Nano silver-porcine acellular dermal matrix (PADM) biological dressing and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. Preparation of nano silver particles:

[0036] (1) 1×10 -3 mol / LAgNO 3 Solution: accurately weigh out 0.085gAgNO 3 Place in a beaker, dissolve with sub-boiling water, transfer to a 500mL volumetric flask, and dilute to volume. After filtering to remove impurities, add it to a container rinsed with sub-boiling water;

[0037] (2) 10mg / mLNa 3 C 6 H 5 O 7 ·2H 2 O solution: accurately weigh 0.1gNa 3 C 6 H 5 O 7 ·2H 2 Place O in a beaker and dissolve it with sub-boiling water, transfer to a 10 mL volumetric flask, and dilute to volume. Filter to remove impurities;

[0038] (3) Heat and stir to contain AgNO 3 Solution container, after boiling, add Na dropwise 3 C 6 H 5 O 7 ·2H 2 The O solution is reacted for about 30 minutes, removed from the heat source, and naturally cooled to obtain a nano silver particle solution with an average particle size of about 70 nm.

[0039] 2. Treatment of porcine acellular dermal matrix (PADM):

[0040] (1) Put the purchased PADM in 75% alcohol for ...

Embodiment 2

[0053] 1. Preparation of nano silver particles:

[0054] (1)0.5×10 -3 mol / LAgNO 3 Solution: accurately weigh out 0.042gAgNO 3 Place in a beaker, dissolve with sub-boiling water, transfer to a 500mL volumetric flask, and dilute to volume. After filtering to remove impurities, add it to a container rinsed with sub-boiling water;

[0055] (2) 5mg / mLNa 3 C 6 H 5 O 7 ·2H 2 O solution: accurately weigh out 0.05gNa 3 C 6 H 5 O 7 ·2H 2 Place O in a beaker and dissolve it with sub-boiling water, transfer to a 10 mL volumetric flask, and dilute to volume. Filter to remove impurities;

[0056] (3) Heat and stir to contain AgNO 3 Solution container, after boiling, add Na dropwise 3 C 6 H 5 O 7 ·2H 2 O solution, after reacting for about 20 minutes, remove from the heat source and cool naturally to obtain a nano silver particle solution with an average particle size of 55-75 nm.

[0057] 2. Pretreatment of porcine acellular dermal matrix:

[0058] (1) Put the purchased PADM in 75% alcohol for 6 hou...

Embodiment 3

[0067] 1. Preparation of nano silver particles:

[0068] (1) 1.5×10 -3 mol / LAgNO 3 Solution: accurately weigh out 0.085gAgNO 3 Place in a beaker, dissolve with sub-boiling water, transfer to a 500mL volumetric flask, and dilute to volume. After filtering to remove impurities, add it to a container rinsed with sub-boiling water;

[0069] (2) 15mg / mL Na 3 C 6 H 5 O 7 ·2H 2 O solution: accurately weigh 0.15gNa 3 C 6 H 5 O 7 ·2H 2 Place O in a beaker and dissolve it with sub-boiling water, transfer to a 10 mL volumetric flask, and dilute to volume. Filter to remove impurities;

[0070] (3) Heat and stir to contain AgNO 3 Solution container, after boiling, add Na dropwise 3 C 6 H 5 O 7 ·2H 2 O solution, react for about 40 minutes, remove from the heat source, and cool naturally to obtain a nano silver particle solution with an average particle size of 55-75 nm.

[0071] 2. Pretreatment of porcine acellular dermal matrix:

[0072] (1) Put the purchased PADM in 75% alcohol for 6 hours, and u...

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Abstract

The invention discloses a nano silver-porcine acellular dermal matrix (PADM) biological dressing and a preparation method thereof. The biological dressing comprises PADM and nano-silver particles assembled on the PADM, wherein, the content of the nano-silver particles is 435.66-447.66 mu g / g, and the particle size of the nano-silver particles is 55-75nm. The preparation method of the biological dressing comprises the following steps: (1) reducing silver nitrate with trisodium citrate to prepare nano silver solution; (2) carrying out gradient dehydration with alcohol and ultrasonic treatment on the PADM; and (3) placing the treated PADM obtained from the step (2) in the nano silver solution for self-assembly, thus finally obtaining the nano silver-PADM biological dressing. The biological dressing obtained in the invention has the advantages of strong antibacterial property, high biological safety and good tissue compatibility; and simultaneously, the process is simple, and the use is convenient.

Description

Technical field [0001] The invention belongs to the field of medical devices, and particularly relates to a nano-silver-porcine acellular dermal matrix biological dressing and a preparation method thereof. Background technique [0002] The skin is a barrier to maintain the stability of the internal environment and prevent the invasion of microorganisms. Rebuilding or restoring the skin barrier is the ultimate goal of burn treatment. Before reaching this goal, a wound covering with excellent performance can temporarily function as a skin barrier and provide an environment conducive to wound healing. An ideal wound cover should have: ①Good adhesion and permeability, and can control water evaporation, and have a water evaporation rate similar to that of normal skin; ②Reduce the loss of nutrients through the wound, prevent bacterial invasion and limit bacteria in Colonization on the wound; ③Relief of pain; ④Good compliance, good elasticity and flexibility, can cover the wound accord...

Claims

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Application Information

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IPC IPC(8): A61L15/44A61L15/40
Inventor 顾海鹰鲁双云夏栋林何红张逸黄桂娟景红霞王雨飞
Owner NANTONG UNIVERSITY
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