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Beautifying and nursing essence and preparation method thereof

A technology of essence and culture solution, which is applied in the fields of biotechnology and cosmetics, can solve the problems of limited beauty and maintenance effects, and achieve the effects of improving phagocytosis, protecting integrity and promoting metabolism

Inactive Publication Date: 2014-07-09
奥思达干细胞有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the fibroblast liquid has the effect of promoting the metabolism of epidermal cells, its beauty and maintenance effect is limited

Method used

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  • Beautifying and nursing essence and preparation method thereof
  • Beautifying and nursing essence and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: A kind of essence that is used for beauty maintenance, this essence is made up of the following components by weight percentage: the epidermal stem cell culture concentrated solution 78.8%, glycerol 20% and hyaluronic acid 0.2% that add various growth factors and vitamins %. Its preparation method comprises the following steps:

[0039] Step 1) Collect neonatal umbilical cord epidermal cells: wash the neonatal umbilical cord tissue with normal saline containing penicillin and streptomycin for 3 times, then cut the umbilical cord tissue into small pieces of 0.2-0.5mm and lay them on the cells cultivated in a petri dish;

[0040] Step 2) Petri dish coating: the epidermal cell culture dish is coated with polylysine at a concentration of 100ug / ml, and used after natural drying;

[0041]Step 3) Epidermal cell culture and subculture: use the primary culture medium for primary culture of the adherent epidermal cells, replace the epidermal cell culture medium ev...

Embodiment 2

[0052] Embodiment 2: Experimental effect test example, take the following scheme:

[0053] Subjects: Volunteers from Shanghai, Suzhou, Wuxi, Jiangyin and Yixing, whose facial skin has spots and wrinkles due to acne, skin inflammation, scars or aging. The experiment excludes women who are pregnant, planning to be pregnant or breastfeeding, and patients with other skin diseases, such as solar dermatitis, facial psoriasis, seborrheic dermatitis, and urticaria. The age is 25-40 years old, 42 males and 68 females . During the clinical observation period, the volunteers were prohibited from eating spicy and stimulating food, quitting smoking and drinking. Volunteers did not use hormonal drugs, anti-allergic drugs, or auxiliary skin care products from 2 weeks before the clinic. Volunteers participated in the experiment voluntarily and signed an informed consent form.

[0054] Experimental method: Professional dermatologists inspected volunteers' facial skin lesions and took photos...

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Abstract

The invention discloses a beautifying and nursing essence and a preparation method thereof. The essence is prepared from an epidermis cell nutrient solution and contains a plurality of cell growth factors, amino acids and vitamins. The essence mainly comprises an epidermal growth factor, a fibroblast growth factor, collagen type I, a stem cell growth factor, a horn cell growth factor and amino acids and vitamins including gamma-aminobutyric acid, coenzyme Q, medical vitamin C, medical vitamin B5 and medical vitamin E. The preparation method for the essence comprises the following steps: acquisition of epidermis of umbilical cord tissue of a newborn; cell culture; collection of a cell nutrient solution; filtration sterilization; condensation of a volume; addition of active components; addition of glycerin and hyaluronic acid; etc. The beautifying and nursing essence provides a plurality of endogenous nutritional ingredients with physiological activity for the skin, can promote metabolism of epidermis cells and repair damaged cells, has a moisture retention effect and is applicable to skin beautifying and nursing.

Description

technical field [0001] The invention relates to a biological cosmetic and a preparation method thereof, in particular to a human epidermal cell culture essence for beauty maintenance and a preparation method thereof, belonging to the fields of biotechnology and cosmetics. Background technique [0002] In recent years, a large number of skin care essences have emerged in the beauty market. However, the active ingredients of existing essences are mostly plant extracts, and the liquid itself belongs to fine chemical products, and in order to improve the beauty effect of cosmetics, most of them contain harmful substances such as lead, mercury, alcohol, preservatives, hormones, spices, etc. substance. When consumers use essence to maintain their skin, it also causes great damage to the skin, and even produces problems such as spots, redness, and allergies, which poses a great threat to consumers' health. In addition, most of the existing products are refined by chemical methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/98A61K8/65A61K8/64A61K8/67A61K8/73A61Q19/00
Inventor 周萱
Owner 奥思达干细胞有限公司
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