134 results about "STEM CELL GROWTH FACTOR" patented technology

The present invention provides a class of interpenetrating polymeric networks (IPNs) and semi-interpenetrating polymeric networks (sIPNs) which include a covalently grafted growth factor or differentiation factor for a stem cell.

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.

The invention provides application of human steam cell growth factors in cosmetics. In an application method, human cell growth factors are added into the cosmetics by a homogenizing process, wherein the human steam cell growth factor content in each gram of cosmetics is between 0.1 and 20.0 ng, and the purity is between 85 and 99 percent. The specific application method comprises the following steps of: dividing components which need to be heated in raw materials of the cosmetics into an oil-based component and a water-based component, heating respectively, and mixing; and homogenizing and emulsifying, cooling to the temperature of between 37 and 50 DEG C, adding solution containing the human steam cell growth factors and components which do not need to be heated, stirring uniformly, and performing vacuum degassing. In the application method, the human steam cell growth factors are applied to the cosmetics for the first time, the human steam cell growth factors still can keep excellent activity in a cosmetic matrix material, and the wrinkle resistance of the cosmetics containing the human steam cell growth factors is still high after the cosmetics are stored for one month.

The present invention provides a method for expanding an endothelial progenitor cell in vitro. More particularly, the present invention provides a method for culturing a hemangioblast comprising incubating a hemangioblast in a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin, and a vascular endothelial cell produced by the method; and a serum-free culture medium containing one or more factors selected from the group consisting of stem cell growth factor, interleukin-6, FMS-like tyrosine kinase 3 and thrombopoietin, and a kit for the preparation of the serum-free culture medium and the like.

The invention discloses an HSCGF (Human Stem Cell Growth Factor) liposome as well as the preparation and the application thereof. The HSCGF liposome comprises 2.0 to 4.0 percent of HSCGF, 8.0 to 10.0 percent of phosphatide, 2.5 to 3.5 percent of cholesterol and 10 to 25 percent of protecting agents. The HSCGF is prepared into liposome, the activity of the HSCGF is kept effectively, the stability of SCGF (Stem Cell Growth Factor) can be improved obviously, skin can be promoted to absorb and utilize the SCGF, and the HSCGF liposome has the advantages of good affinity, no toxicity or side effects, and wide application range. The anti-wrinkle performance of cosmetics containing the HSCGF coated with the liposome can still be kept well after the stability is examined for three months. The HSCGF liposome is used as raw material and other active materials and auxiliary materials are used as supplements so as to prepare frequently-used medicine forms to illustrate the drug effect of the SCGF, so that the bioavailability can be improved.

The invention relates to a medical dressing with bioactivity and a preparation method of the medical dressing and belongs to the technical field of biomedical engineering and material science. The medical dressing with the bioactivity is used for covering skin defect wounds caused by burning, scalding, operation, wounds and diseases and inducing wound repair and is prepared as follows: one or more of embryonic stem cells, umbilical cord blood stem cells, amniotic fluid stem cells, peripheral blood stem cells and bone mesenchymal stem cells of humans or animals are cultured in vitro, stem cell growth factors secreted in the logarithmic growth phase are collected and mixed in proportion with one or more of I type collagen, III type collagen, chitosan, hyaluronic acid, chondroitin sulfate and sodium alginate, thin-layer sponge is taken as an inner layer, a porous high-polymer material film outer layer prepared from a mixture formed by one or more of PLA (polylactic acid), PGA (polyglycolic acid), PLGA (poly(lactic-co-glycolic acid)) and PCL (polycaprolactone) is attached, the product is frozen-dried and cut, and the medical dressing with the bioactivity is formed and used for treating skin defect wounds caused by burning, scalding, operation, wounds and diseases and inducing wound repairing.

The invention discloses a beautifying and nursing essence and a preparation method thereof. The essence is prepared from an epidermis cell nutrient solution and contains a plurality of cell growth factors, amino acids and vitamins. The essence mainly comprises an epidermal growth factor, a fibroblast growth factor, collagen type I, a stem cell growth factor, a horn cell growth factor and amino acids and vitamins including gamma-aminobutyric acid, coenzyme Q, medical vitamin C, medical vitamin B5 and medical vitamin E. The preparation method for the essence comprises the following steps: acquisition of epidermis of umbilical cord tissue of a newborn; cell culture; collection of a cell nutrient solution; filtration sterilization; condensation of a volume; addition of active components; addition of glycerin and hyaluronic acid; etc. The beautifying and nursing essence provides a plurality of endogenous nutritional ingredients with physiological activity for the skin, can promote metabolism of epidermis cells and repair damaged cells, has a moisture retention effect and is applicable to skin beautifying and nursing.

The invention discloses a serum-free medium of a stem cell, which comprises a basal medium and an additive, wherein the basal medium is DMEM / F12 (Dulbecco Modified Eagle Medium / F12); the additive comprises 2-15%(v / v) of serum replacement, 20-100ug / ml vitamin C, 0.5-10ng / ml stem cell growth factor, 5-20ng / ml human platelet-derived growth factor and 1-5mmol / ml L-glutamine. The serum-free medium of the stem cell for cell culture has the advantages of short cell cycle, strong multiplication capacity, good cell uniformity and high purity, effectively inhibits differentiation of the stem cell and adherence growth of an endothelial cell, ensures purity and dryness of the stem cell and is free from ingredients of animal origin such as fetal calf serum, and the stem cell obtained by the medium is suitable for clinical application.

The invention provides a serum-free adipose-derived stem cell culture medium, belonging to the technical field of stem cells. The serum-free adipose-derived stem cell culture medium comprises an IMDM (Iscove's modified Dulbecco's medium) basal culture medium, recombinant human insulin, recombinant transferrin, vitamin C, human serum albumin, fibroblast growth factor, platelet-derived growth factor, epidermal growth factor, insuline-like growth factor, transforming growth factor, fiber connexin, fetuin, tea polyphenol and stem cell growth factor. The serum-free adipose-derived stem cell culture medium provided by the invention does not need to perform culture bottle coating, and has the advantages of cell adherent property, favorable morphology and high cell proliferation rate.

The invention discloses an injection gel containing submicron hyaluronic acid microspheres and a preparation method thereof. The diameter of each microsphere is 100 nanometers to 50 micrometers. Swelling ratio of the injection gel is 2-2000. The preparation method includes: dissolving biodegradable hyaluronic acid into organic solvent to obtain spinning solution; electrospinning the spinning solution using electrostatic spinning, and crosslinking through coagulating bath to obtain nano / submicron microspheres; dialyzing to purify the microspheres, preparing into injection with phosphate buffer or normal saline, and homogenizing with suspension of a stem cell growth factor system according to a certain volume ratio to obtain the injection gel. The injection gel containing submicron hyaluronic acid microspheres prepared by the method is fine in shaping performance, long in shape preservation, and fine in cell compatibility, and is applicable as therapeutic for ophthalmology and orthopedics, also applicable as tissue-engineered repair materials such as bone tissues, cartilage tissues, blood vessel tissues, heart tissues and nerve tissues or as filling material for plastic surgery.

The invention provides a serum-free medium for human umbilical cord mesenchymal stem cells and belongs to the technical field of stem cells. The serum-free medium comprises a DMEM basic medium and further comprises recombinant human insulin, human serum albumin, transferrin, fibronectin, vitamin C, biotin, stem cell growth factors and stem cell factors. The serum-free medium for human umbilical cord mesenchymal stem cells provided by the invention needs no enveloping of a culture flask, operating is convenient and simple, cell proliferation rate is high, good stem cell pedomorphism and stem cell characteristics are kept, the serum-free medium has a good potential for cell induced differentiation, and the cost is lowered.

The invention discloses a method and a special culture medium for subculturing chicken embryonic stem cells for a long time. The method is characterized by comprising the following steps of: isolating cells of area pellucida of X-stage blastoderm, dispersing into single cells or small cell masses through mechanical blowing and beating, inoculating on an STO feeder layer, culturing the chicken embryonic stem cells in the special culture medium at the temperature of between 37 and 38 DEG C, and subculturing once every 3 to 5 days, wherein the special culture medium comprises 600 to 900mL of conditioned medium, 50 to 150mL of fetal calf serum, 5 to 20mL of chicken serum, 5 to 25mL of one or a mixture of more of non-essential amino acids, 0.146 to 0.292g of L-glutamine, 6 to 14mu L of beta-mercaptoethanol, 1 to 2*10<6> IU of mouse leukemia inhibitory factor, 10 to 50mu g of alkaline fibroblastic growth factor and 5 to 20mu g of stem cell growth factor; and fixing the volume of the ingredients to 1,000mL by using a dulbecco's modified eagle medium (DMEM) (high glucose), and regulating the pH value to 7.2 to 7.5. The conditioned medium is obtained by the steps of: culturing BRL-3A cells until the cells are converged, collecting supernatant of the cultured cells, performing centrifugal separation, and filtering by using a filter membrane to obtain the conditioned medium. Compared with the prior art, the method has the advantages that: the long-term subculture of the chicken embryonic stem cells can be realized, the proliferation of the cells is quick, and the cloning yield is high.

The invention relates to a biological preparation for promoting quick growth and repair of a skin tissue. The preparation comprises the components in parts by weight as follows: 20-50 parts of active growth factor group and nutritional components and 1 part of biological general flavone, wherein the active growth factor group and nutritional components comprise epidermal growth factors (EGF), nerve growth factors (NGF), vascular endothelial growth factors (VEGF), stem cell growth factors (SCF), hepatocyte growth factors (HGF), transforming growth factors (TGF), fibroblast growth factors (bFGF), collagen, hyaluronic acids, polypeptide, nucleic acids and amino acids; the source for extracting the active growth factor group and nutritional components is original stem cells separated from cord blood or umbilical cord or placenta of new-born; and the biological general flavone is extracted from natural Chinese herbal medicines. The preparation provided by the invention is a pure natural biological preparation and can promote quick growth and repair of the skin tissue.

The invention discloses a preparation method for the dendritic cell (DC) of an umbilical cord blood source and a dendritic cell (DC) vaccine, which relates to a preparation method for the dendritic cell. According to the method, various cell factors are adopted to induce DC obtained by umbilical cord blood separation, and then the DC is stimulated by a tumor specific antigen so as to improve the specific antigen presentation capability of the DC; and a stem cell growth factor and Flt3-L are added into a cell culture medium so as to effectively accelerate a hematopoietic cell in the umbilical cord blood to induce and proliferate to an immune cell. The DC vaccine prepared with the method has the specific antigen presentation capability, can be combined with a CIK (cytokine induced killer) cell to mutually treat the malignant tumor when being used as a tumor immunotherapy product, and is used as an important adjuvant therapy after operations and chemoradiotherapy. Recurrence and metastasis after the operations can be effectively prevented, and toxic and side effects caused by the chemoradiotherapy on patients are lowered so as to improve the treatment effect.

The invention provides a human skin epidermal cell culture medium and application thereof. The culture medium is prepared by the following steps: mixing a K-SFM (keratinocyte serum-free medium), a DMEM (dulbecco's modified eagle medium) and a F12 culture medium according to the ratio of 2:1:1, and adding a BPE (bovine pituitary extract), an EGF (epidermal growth factor), an SCGF (stem cell growth factor), an FGF (fibroblast growth factor), a TGF-beta (transforming growth factor-beta), a VEGF (vascular endothelial growth factor), CaCl2, glutamine and a double-antibody. The culture medium is free of serum, and can be used for human skin epidermal cell primary culture and subculture. The human skin epidermal cell culture medium can obtain abundant epidermal cells by in-vitro culture by using only a small amount of patient skin tissues. Compared with the traditional epidermal cell culture medium, the human skin epidermal cell culture medium provided by the invention greatly shortens the time required by amplifying the same cell count, and satisfies the demands for clinical therapy.

The invention belongs to the technical field of stem cells and relates to a serum-free medium for placenta-derived mesenchymal stem cells and a preparation method thereof. The serum-free medium comprises a DMEM basic medium and further comprises vitamin H, glutathione, recombinant human insulin, human serum albumin, transferrin, fibroblast growth factors, epidermal growth factors, stem cell growth factors, Human stem cell factors and magnolol. The serum-free medium provided by the invention has the advantages of high safety and capability for significant improvement of proliferative capability of placenta-derived mesenchymal stem cells, the serum-free medium is capable of well keeping cell forms and stem cell characteristics of the placenta-derived mesenchymal stem cells and is convenient to popularize and apply.

The invention relates to a liposome face-nourishing skin care product, belonging to the technical field of cosmetics. The liposome face-nourishing skin care product comprises the following components in parts by weight: 0.5-8.0 parts of epidermal growth factor (EGF), 0.01-0.1 part of human stem cell growth factor (SCGF), 10.0-10,000.0 parts of hyaluronic acid, 0.003-3000.0 parts of phospholipid, 0.001-10.0 parts of metallothionein, 0.001-10.0 parts of collagen, 0.01-100.0 parts of male larva, and 0.001-200.0 parts of cholesterol. The skin care product provided by the invention has the beneficial effects that the liposome is composed of phospholipid bilayers and contains pure water micro vesicles, and the vesicles can enclose a certain amount of nutrients and essences, are 100 nanometers in mean diameter which is 1 / 200-1 / 300 of the human cell diameter, can easily penetrate through the surface layer of human skin and can be fused with cells, thus exerting efficacy. The skin care product can deeply humidify horny cells and enhance hydration to improve the skin softness, and can provide sufficient nutrients to ensure skin health and increase skin resistance.

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.

The invention belongs to the technical field of stem cells, and relates to an ex-vivo expansion culture medium of umbilical cord blood hematopoietic stem cells and an application thereof. The ex-vivo expansion culture medium comprises an IMEM basal culture medium, serum, thrombopoietin, stem cell factors, FMS-liketyrosinc kinase 3 ligand, interleukin-1, interleukin-6, stem cell growth factors and berberine. The ex-vivo expansion culture medium of the umbilical cord blood hematopoietic stem cells has the advantages of being high in hematopoietic stem cell proliferation rate, capable of significantly improving the implantation capacity of the hematopoietic stem cells transplanted into a receptor and the reconstruction capacity of a hematopoietic system and capable of well maintaining the properties of the hematopoietic stem cells and is an ideal ex-vivo expansion culture medium of the umbilical cord blood hematopoietic stem cells.

The invention relates to a liposome sun-screening skin care product, belonging to the technical field of cosmetics. The liposome sun-screening skin care product comprises the following components in parts by weight: 0.5-8.0 parts of epidermal growth factor (EGF), 0.01-0.1 part of human stem cell growth factor (SCGF), 10.0-10,000.0 parts of hyaluronic acid, 0.003-3000.0 parts of phospholipid, 0.001-10.0 parts of metallothionein, 0.001-10.0 parts of collagen, 10.0-5000.0 parts of water-soluble pearl powder the weight percentage of which is 20%, and 0.001-200.0 parts of cholesterol. The sun-screening skin care product provided by the invention has the beneficial effects that the liposome is composed of phospholipid bilayers and contains pure water micro vesicles, and the vesicles can enclose a certain amount of nutrients and essences, are 100 nanometers in mean diameter which is 1 / 200-1 / 300 of the human cell diameter, can easily penetrate through the surface layer of human skin and can be fused with cells, thus exerting efficacy. The liposome sun-screening skin care product can deeply humidify horny cells and enhance hydration to improve the skin softness, and can provide sufficient nutrients to ensure skin health and increase skin resistance.

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. In particular, the invention relates to novel stem cell growth factor-like polypeptides, including novel proteins named SCGF3248Fk081_aa2, SCGF3248Fk081_aa1, SCGFFk081_aa3, and SCGF323401Fe131_aa1. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.

The invention provides an endothelial cell culture medium and an endothelial cell culture method, wherein the endothelial cell culture medium comprises a serum-free basal culture medium, insulin, transferin, vitamin C, bovine serum albumin, a basic fibroblast growth factor, a blood vessel endothelium growth factor, a stem cell growth factor, laminin, an epidermal growth factor, non-essential amino acid and melbinum hydrochloride. By virtue of compatibility of ingredients, the endothelial cell culture medium provided by the invention is capable of improving the endothelial cell multiplication capacity. In addition, the endothelial cell culture medium provided by the invention is further capable of improving the blood vessel formation capability of endothelial cells. The experimental result indicates that when endothelial cells are cultured to the P10 generation by virtue of the endothelial cell culture medium provided by the invention, the cells still grow vigorously; the P10-generation endothelial cell phenotype CD309 and CD31 double-positive expression is as high as 97.2%.

The invention provides an inflammation diminishing and acne removing mask. Hydrogel is adopted as the mask matrix, and the hydrogel contains a stem cell growth factor, a honeysuckle flower extract, an aloe extract, and pearl powder. The inflammation diminishing and acne removing mask provided by the invention takes hydrogel as the mask matrix, and integrates the stem cell growth factor, the honeysuckle flower extract, the aloe extract, and pearl powder, has good acne removing, inflammation diminishing and aging defying effects. The invention provides a preparation method of the inflammation diminishing and acne removing mask. The method consists of: mixing the honeysuckle flower extract, the aloe extract, pearl powder, the stem cell growth factor powder and hydrogel to obtain a mask liquid; and preparing the mask liquid into a mask, thus obtaining the inflammation diminishing and acne removing mask. The mask prepared by the method provided by the invention has high absorption efficiency during use, and has good acne removing, scar fading and anti-aging effects.

The invention relates to the technical field of cell culture, in particular to a culture medium, application thereof and a culture method. The culture medium comprises transferrin, recombinant human insulin, progesterone, fetal calf serum, stem cell growth factors, vitamin C, interleukin 2, interleukin 4, zinc sulfate and a DMEM / F12 culture medium. When the culture medium is used for culturing CD19-CAR-T cells, the cell proliferation multiple can be improved, the number of cell culture days is increased, and the CD19CAR and other surface markers can be kept expressed.

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human and mouse secreted stem cell growth factor-like polypeptide. These polynucleotides comprise nucleic acid sequences isolated from cDNA libraries prepared from mouse bone marrow and human fetal liver spleen, ovary, adult brain, lung tumor, spinal cord, cervix, ovary, endothelial cells, umbilical cord, placental, lymphocyte, lung fibroblast, fetal brain, and testis. Other aspects of the invention include vectors containing processes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides.

The invention discloses a preparation method and application of stem cell culture solution containing wash-free mask liquid. The mask liquid is prepared from, by weight, 0.2%-0.5% of high molecular weight sodium hyaluronate, 1%-5% of glycerin, 0.1%-2.0% of a stem cell culture solution, 0.05%-0.5% of nanosized sodium hyaluronate, 0.3%-1% of 1,2-hexanediol, 0.3%-0.5% of p-hydroxyacetophenone, 0.05%-0.2% of xanthan gum, 1%-5% of moringa seed extracts and the balance deionized water. Accordingly, the mask liquid contains the stem cell culture solution which contains a larger number of growth factors, ultra-low molecular weight nanosized sodium hyaluronate and the moringa seed extracts can make the skin care effect of the stem cell culture solution brought into full play, stem cell growth factors go deep into the skin dermis and subcutaneous tissue to comprehensively activate stem cell differentiation inside the skin when nanosized sodium hyaluronate is subjected to transdermal absorption, stem cell differentiation inside the skin is comprehensively activated, senescent cells are rapidly updated and replaced, the skin is improved, moisturizing and elasticity are improved, and wrinkles are reduced. Accordingly, cleaning is not needed after a mask is used.