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136 results about "Feeder Layer" patented technology

A layer of supportive cells that are irradiated to prevent replication but can still secrete growth enhancing factors for use in co-culture

Episomal reprogramming with chemicals

Methods and composition of induction of pluripotent stem cells are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells with cell signaling regulators are described. Furthermore, certain aspects of the invention provide novel compositions comprising induced pluripotent stem cells essentially free of exogenous retroviral vector elements in the presence of a medium comprising signaling inhibitors. In certain aspects, feeder-free episomal reprogramming methods may be provided.
Owner:FUJIFILM CELLULAR DYNAMICS INC

Dynamic layer diagnostic device and method of smart power grid fault

A dynamic layer diagnostic device for smart power grid fault comprises a data collection and monitoring unit, a data processing unit, a data base unit, a communication unit and a man-machine interaction unit. A dynamic layer diagnostic method includes: when a smart power grid breaks down, calculating fault diagnosis starting conditions to conform a diagnosis strategy, wherein the fault diagnosis strategy comprises switch layer diagnosis, feeder layer diagnosis and transformer substation layer diagnosis; starting the switch layer diagnosis when changes of switch motion information are remarkable before and after the fault; starting the transformer substation diagnosis when changes of electricity amount information are remarkable before and after the fault; stopping the diagnosis when a fault element is the only one element during the diagnosis of the switch layer; otherwise, retrieving the switch historical action recording, and starting the transformer substation diagnosis when recording matched with the current switch action exists; and otherwise, starting the feeder layer diagnosis. The dynamic layer diagnostic method performs layering analysis on the fault, fully utilizes various fault information and improves fault diagnosis accuracy according to different characteristics of multisource information after the power grid fault and difficulty layer in obtaining and processing of various information.
Owner:SHENYANG POWER SUPPLY LIAONING POWER +2

Isolation, expansion and use of clonogenic endothelial progenitor cells

A hierarchy of endothelial colony forming cells (EPCs) was identified from mammalian cord blood, umbilical vein and aorta. A newly isolated cell named high proliferative potential—endothelial colony forming cell (HPP-ECFC) was isolated and characterized. Single cell assays were developed that test the proliferative and clonogenic potential of endothelial cells derived from cord blood, or from HUVECs and HAECs. EPCs were found to reside in vessel walls. Use of a feeder layer of cells derived from high proliferative potential-endothelial colony forming cells (HPP-ECPCS) from human umbilical cord blood, stimulates growth and survival of repopulating hematopoietic stem and progenitor cells. Stimulation of growth and survival was determined by increased numbers of progenitor cells in in vitro cultures and increased levels of human cell engraftment in the NOD / SCID immunodeficient mouse transplant system.
Owner:INDIANA UNIV RES & TECH CORP

High-density integration tile-type active phased-array antenna structure

The invention discloses a high-density integration tile-type active phased-array antenna structure, and aims to provide a high-integration tile-type active phased-array antenna structure which is smaller in volume, lighter in weight, lower in cost and stable in performance and can eliminate a self-excitation phenomena. The structure is realized through the following technical scheme: a radiation array element layer is arranged on an upper multi-layer PCB (2), each metal radiation patch (1) array module is electrically connected to an MMIC chip (5) through a radio-frequency feeder line (4), the MMIC chips are packaged in metal sealed cavities (12) between the upper multi-layer PCB and a lower multi-layer PCB to form the RF feeder layer; each MMIC chip is electrically connected to a channel assembly (7), a power supply assembly (8) and a beam control assembly (9) which are positioned on the lower bottom plane of a multi-layer printed board (3) respectively, and an DC power supply and a control circuit layer are formed by the MMIC chips as well as a radio-frequency external interface (10) and a control and power supply external interface (11) which are arranged at two ends of the bottom of the multi-layer printed board respectively.
Owner:10TH RES INST OF CETC

Cell culture platform for single cell sorting and enhanced reprogramming of ipscs

ActiveUS20140220681A1Increased potencyImproving efficiency of reprogrammingCell dissociation methodsGenetically modified cellsFeeder freeCell dissociation
The invention provides cell culture conditions for culturing stem cells, including feeder-free conditions for generating and culturing human induced pluripotent stem cells (iPSCs). More particularly, the invention provides a culture platform that allows long-term culture of pluripotent cells in a feeder-free environment; reprogramming of cells in a feeder-free environment; single-cell dissociation of pluripotent cells; cell sorting of pluripotent cells; maintenance of an undifferentiated status; improved efficiency of reprogramming; and generation of a naïve pluripotent cell.
Owner:FATE THERAPEUTICS

Six-column differential-pressure distillation device for extra edible alcohol and process therefor

The invention discloses a six-tower distilling device and technology of superfine edible spirit, which comprises the following parts: crude distillation column, vacuum aldehydo-removing column, water scrubber, fractionating tower, methanol column and recycling tower, wherein the mature ferment fermented glutinour rice is preheat in the ferment procedure, which enters into the top of crude distillation column; the crude distillation column is negative pressure, whose top wine gas enters in the vacuum aldehydo-removing column through separator after cooling sequently; the aldehydo-removing wine on the bottom is sent into feeder layer of water scrubber after preheating, which flows desalination wine after extracted by micro-negative pressure water in the fractionating tower to proceed positive pressure distillation; the spirit intermediate product is led into the methanol column from top wine hole of fractionating tower to remove menthol, which obtains superfine edible spirit; the steam condensate of fractionating tower reboiler is recycled in the autoclave of recycling tower after flash-boiling disposal, which flows through methanol column reboiler, water scrubber and crude distillation column to proceed secondary condensing by reboiler.
Owner:GUANGDONG ZHONGKE TIANYUAN NEW ENERGY SCI & TECH

Method and special culture medium for subculturing chicken embryonic stem cells for long time

The invention discloses a method and a special culture medium for subculturing chicken embryonic stem cells for a long time. The method is characterized by comprising the following steps of: isolating cells of area pellucida of X-stage blastoderm, dispersing into single cells or small cell masses through mechanical blowing and beating, inoculating on an STO feeder layer, culturing the chicken embryonic stem cells in the special culture medium at the temperature of between 37 and 38 DEG C, and subculturing once every 3 to 5 days, wherein the special culture medium comprises 600 to 900mL of conditioned medium, 50 to 150mL of fetal calf serum, 5 to 20mL of chicken serum, 5 to 25mL of one or a mixture of more of non-essential amino acids, 0.146 to 0.292g of L-glutamine, 6 to 14mu L of beta-mercaptoethanol, 1 to 2*10<6> IU of mouse leukemia inhibitory factor, 10 to 50mu g of alkaline fibroblastic growth factor and 5 to 20mu g of stem cell growth factor; and fixing the volume of the ingredients to 1,000mL by using a dulbecco's modified eagle medium (DMEM) (high glucose), and regulating the pH value to 7.2 to 7.5. The conditioned medium is obtained by the steps of: culturing BRL-3A cells until the cells are converged, collecting supernatant of the cultured cells, performing centrifugal separation, and filtering by using a filter membrane to obtain the conditioned medium. Compared with the prior art, the method has the advantages that: the long-term subculture of the chicken embryonic stem cells can be realized, the proliferation of the cells is quick, and the cloning yield is high.
Owner:FOSHAN UNIVERSITY

Tissue engineering skin construction method and tissue engineering skin produced by the same

The invention discloses a method for constructing tissue engineering skin, which comprises the steps as follows: (1) a tissue engineering skin nutrient solution is prepared; (2) a demic mechanocyte feeder layer is prepared; (3) a gelatin biomaterial is prepared; (4) the tissue engineering skin is constructed and cultivated. The method for constructing the tissue engineering skin adopts the adding of the mechanocytes after proliferation inhibition treatment to complete the interaction with epidermic cells so as to promote the mature and differentiation degree of the epidermic cells, has significant effect on improving the accuracy of compound toxicity detection in vitro to ensure that the prepared tissue engineering skin can more effectively resist the toxic action of the compound on competent cells and the forecast precision of the prepared tissue engineering skin to the compound toxicity is improved so as to create more effective animal substitution detection methods which are based on the tissue engineering skin.
Owner:CHINESE ACAD OF INSPECTION & QUARANTINE

Cultivation of Primate Embryonic Stem Cells

The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferrin, insulin, albumin, and a fibroblast growth factor that is supplied from a source other than just a feeder layer the medium. Also disclosed are compositions capable of supporting the culture and proliferation of human embryonic stem cells without the need for feeder cells or for exposure of the medium to feeder cells.
Owner:WISCONSIN ALUMNI RES FOUND
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