218 results about "Eagle" patented technology

The invention relates to the field of health-care, and in particular relates to a culture method of hair follicle stem cell for treating baldness, as well as a syringe. The culture method comprises the following steps: A, digesting excised hair follicles by utilizing separase; B, cutting bugle of follicles; C, digesting the follicle bugle by utilizing pancreatin; D, centrifugally collecting the follicle bulge obtained in the step C to obtain cells; E, culturing the cells in DEME (Dulbecco's Modified Eagle's Medium) culture solution, and primarily separating; F, culturing the product which is obtained in primary separation of the step E in a culture dish; and G, centrifugally collecting upper unattached keratinocyte and follicle fibroblast which are cultured in the step F, and culturing to obtain hair follicle stem cells. The syringe comprises a hair follicle stem cell extraction component and a syringe needle, wherein the hair follicle stem cell extraction component is used for quantitatively extracting hair follicle stem cells, and connected with the syringe needle; and the syringe needle is bent and blunt. According to the method and the syringe, hair growth under baldness situation can be improved.

A composition for cryopreservation of cells and tissues of human and other animals in a safe manner without using toxic substances such as DMSO, as well as for freeze preserving or freeze-drying of foods and pharmaceuticals. In examples, ε-poly-L-lysine is reacted with succinic anhydride so that 60% or more of amino groups are blocked; and, thus obtained polymer compound is added to Dulbecco-modified eagle MEM culture medium (DMEM) on market sale to form a cryopreservation liquid. In examples for foods or pharmaceuticals, the ε-poly-L-lysine derivative was added by 0.5-10 wt % to curb freeze concentration.

The invention discloses an electric-gas compound driving flexible finger eagle-claw-imitating logistics packaging manipulator comprising a driving part and four same finger parts. Each finger part comprises a finger root joint, a middle finger joint and a fingertip joint. According to the manipulator, the acting point of contact force and the direction of acting force can be selected according toconditions, thus an object is not deformed or damaged in the grabbing process, and grabbing is more accurate and reliable. Meanwhile the angles of the finger root joints and the grabbed object are adjusted, can adapt to large change of the size of the grabbed object and adapt to the change of the shape and vertical and transverse placing of the grabbed object, the acting point and magnitude of thegrabbing force have flexible self-adaptability, response is fast, and buffering performance is good.

The invention discloses a method for sequential culture of human umbilical cord blood mesenchymal stem cells by using two culture media. A lymphocyte separation medium density gradient centrifugation method is used for separating umbilical cord blood mononuclear cells, a dulbecco modified eagle medium (DMEM) / F12 is used for primary culture of the mesenchymal stem cells of the human umbilical cord blood, and the method increases adherence of the cells, reduces growth of osteoclast like cells remarkably, facilitates formation of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) colonies, and greatly improves successful rate of culture of the hUCB-MSCs. The DMEM / F12 is used continuously for subculturing to a P2 generation, the method is combined by a using enzymatic digestion and differential velocity adherent method simultaneously, and the method facilitates purification of P1-P2 generation cells remarkably. A P3 generation and the following generations are cultured by using an Oricell human umbilical cord mesenchymal stem cell culture medium, marker proteins and good morphological characteristics and growth characteristics of the hUCB-MSCs are maintained, and multilineage differentiation potential of the hUCB-MSCs is maintained. In addition, costs of the culture media adopted by the method are reduced remarkably.

The invention discloses a quantum-dot-based method for carrying out in-situ and real-time detection on heavy metal ions in cells. The method comprises the following steps of: A. preparing a carboxymethyl chitosan-CdTe quantum dot solution with certain concentration; B. digesting canine kidney cells overgrowing in a T25 cell culture flask bottle into a unicellular suspension, inoculating into a cell culture dish, and culturing; C. adding the carboxymethyl chitosan-CdTe quantum dot solution into the culture dish, and hatching for a period of time, thereby obtaining the canine kidney cells marked with fluorescence; and D. adding a DMEM (dulbecco's modified eagle medium) nutrient solution containing heavy metal ions with gradient concentration and unknown concentration respectively in washed cells in the culture dish, hatching together with the cells, detecting the average fluorescent intensity of the cells respectively by a flow cytometry, and drawing a standard curve through the changes of the cell fluorescent intensity, so that the concentration of the heavy metal ions in the cells can be calculated. The method is simple and convenient to operate; the fluorescence of marked cells can be quenched regularly as the increase of heavy metal ions, and the real-time and in-situ detection on the outer source heavy metal ions in the cells is realized.

The invention discloses an imitation eagle grabbing system of an air-operated multi-rotor aircraft. Two two-degree-of-freedom bionic motion arms are mounted at the bottom of the multi-rotor aircraft, and each bionic motion arm has a hip joint, a knee joint, an ankle joint and a mechanical gripper for grabbing. The imitation eagle grabbing system carries on motion planning according to bionics, and therefore the rapidity, accuracy and sensibility of double arm grabbing of the multi-rotor aircraft are realized. From the point of view of the bionic motion planning, the imitation eagle grabbing system establishes a mapping relationship between eagles grabbing animals and air operation of the multi-rotor aircraft, realizes the dynamic grabbing task of the multi-rotor aircraft in high-speed motion, and shows the superiority of bionics applied to the related fields of robots. Meanwhile, by related means of bionics, the imitation eagle grabbing system establishes a structural model imitating eagle claws and has a guarantee for effective grabbing tasks.

The invention discloses a preparation method of angiogenesis-facilitating temperature-sensitive hydrogel. The preparation method comprises the following steps: under an ice-bath condition, adding the temperature-sensitive hydrogel powder provided by the invention and used as a raw material into a precooled PBS (phosphate buffer saline) solution, distilled water or DMEM (dulbecco's modified eagle medium), wherein the ratio of the temperature-sensitive hydrogel powder to the precooled PBS solution, distilled water or DMEM is 30mg:1ml, uniformly stirring, and placing the mixture in an environment with the temperature of 37 DEG C, so as to obtain the angiogenesis-facilitating temperature-sensitive hydrogel. The invention further discloses novel temperature-sensitive hydrogel powder and a preparation method thereof. The temperature-sensitive hydrogel powder can be stored for a long time at room temperature, the sterilization method for the temperature-sensitive hydrogel powder is simple, and the temperature-sensitive hydrogel powder can be conveniently produced in a large-scale manner and put into a market; the temperature-sensitive hydrogel prepared from the temperature-sensitive hydrogel powder has good temperature sensitiveness and biocompatibility, can be degraded in a body, has an angiogenesis-facilitating effect, and has a good application prospect in regenerative medicine and clinical treatment.

The invention relates to an efficient and reliable simulation machine eagle device which can faithfully protect farmland grains and plants, is driven by solar energy for realizing unmanned and automated farmland protection. The solar-energy machine eagle utilizes solar-energy batteries to generate electrical power, and utilizes a controller to control and drive a direct-current motor to drive a minitype turbofan to rotate at a high speed and generate air flows jetting backwards to impel the model body of the simulation eagle. Meanwhile, the eagle model generates action effects of soaring high up in the air and hovering by buoyancy generated by a hydrogen wing bag and atmosphere lift force caused by elevation angles of two wings and by controlling the pivot angle of a tail rudder and the air content of the left and the right hydrogen wing bags and the front and rear hydrogen wing bags by the controller under the control of a remote controller, frightening and deterring animals such as cocks, birds, mice, and the like from stealing farm crops in farmland, particularly in large area of grainfield which cannot be protected by manpower.

The invention discloses a method and a special culture medium for subculturing chicken embryonic stem cells for a long time. The method is characterized by comprising the following steps of: isolating cells of area pellucida of X-stage blastoderm, dispersing into single cells or small cell masses through mechanical blowing and beating, inoculating on an STO feeder layer, culturing the chicken embryonic stem cells in the special culture medium at the temperature of between 37 and 38 DEG C, and subculturing once every 3 to 5 days, wherein the special culture medium comprises 600 to 900mL of conditioned medium, 50 to 150mL of fetal calf serum, 5 to 20mL of chicken serum, 5 to 25mL of one or a mixture of more of non-essential amino acids, 0.146 to 0.292g of L-glutamine, 6 to 14mu L of beta-mercaptoethanol, 1 to 2*10<6> IU of mouse leukemia inhibitory factor, 10 to 50mu g of alkaline fibroblastic growth factor and 5 to 20mu g of stem cell growth factor; and fixing the volume of the ingredients to 1,000mL by using a dulbecco's modified eagle medium (DMEM) (high glucose), and regulating the pH value to 7.2 to 7.5. The conditioned medium is obtained by the steps of: culturing BRL-3A cells until the cells are converged, collecting supernatant of the cultured cells, performing centrifugal separation, and filtering by using a filter membrane to obtain the conditioned medium. Compared with the prior art, the method has the advantages that: the long-term subculture of the chicken embryonic stem cells can be realized, the proliferation of the cells is quick, and the cloning yield is high.

The invention relates to a glede tea beverage and a preparation method thereof. According to the preparation method, glede tea, which accounts for 0.6 to 0.8 percent of the total weight of the beverage, is crushed into tea powder, sieved by a 50-mesh sieve and then weighed. After that, water accounting for 8 to 10 times of the weight of the glede tea powders is added into an extraction tank, heated to 75 to 90 DEG C at the room temperature, added with the tea powders for stirring extraction for 8 to 12 minutes. Then the materials are filtered by a filter screen being 200 to 300 meshes to obtain filter liquid (1). Water accounting for 4 to 6 times of the weight of the filter residue is adopted for extracting for 4 to 6 minutes under the same conditions of the filter residue. The filtering operation is carried out by the filter screen being 200 to 300 meshes to obtain filter liquid (2). The filter liquids (1) and (2) are merged and added into the extraction tank. The filter liquid is heated to 60 to 80 DEG C and the pressure thereof is reduced the to -15kPa to 20kPa for concentration. Condensed liquid, i.e. aromatic water, is collected. The collecting of the condensed liquid is stopped until the weight of the condensed liquid is 1 to 1.5 times of that of the tea powder. Then concentration is continuously carryed out until the weight is 1 / 4 to 1 / 3 of the total filter liquid weight. The spray drying is carryed out to obtain dry powder. The prepared dry powder, the aromatic water, the white granulated sugar and the citric acid are mixed and prepared. The glede tea beverage has the advantages that the tea beverage has the effects of relieving summer heat and quenching the thirst, helping digestion and enhancing the human body immunity, the quality is easy to control, and in addition, the taste is good.

The invention relates to a construction method of a fin cell line of anabarilius grahami and belongs to the technical field of freshwater aquatic organism cell culture. The method comprises that the anabarilius grahami tail fin tissue cell serves as a material, a tissue block method is adopted, and the primary culture is strated, culturing is achieved in a dulbecco modified eagle medium (DMEM) / F12 containing fetal calf serum and human basic fibroblast growth factors and having a PH value ranging between 7.0 and 7.4; and subculture is performed by using a trypsin digestion method. The method has the advantages that 1, the minimally invasive processing is adopted, on the premise that the fish is alive, and the operation is easy and feasible; 2, the anabarilius grahami fin muscle cell line (AGF) is in a fibroblast cell patternin a fiber structure, continuous passage can be achieved, the line can be applied to biological characteristic research directly, and requirement for anabarilius grahami molecular cell level theoretical research is met; and 3, the construction method is also applicable to cell lines of constructed fin rays of other fishes.

The invention relates to a processing method for eagle tea. The processing method is characterized by comprising the following steps of: (a) spreading out fresh leaves; (b) withering; (c) twisting; (d) fermenting; (e) drying; (f) carrying out stalk extraction; and (g) fragrant baking. According to the traditional processing method for the eagle tea, the fragrance is heavy, the taste is thick and tastes astringent firstly and then sweet, the specific fragrance of Lauraceae plants is obvious, and a tea drinker normally has a feel of 'disgusting taste' in the first time. According to the processing method provided by the invention, the specific processes of withering, fermenting and fragrant baking are utilized, the eagle red is formed, the liquor color is red bright, the eagle tea has specific quality of flavor of wild chrysanthemum flower, and the tea drinker feels as if staying in nature after drinking and feels as if both the mind and the body are sublimated and purified.

The invention discloses a resNet-based small target recognition method for imitating eagle brain feature integration: step 1, establishing a small target recognition map library; 2, initializing thatsetting; 3, normalize images: calculate that mean value and variance of all images in the small target recognition map library, normalizing all images by use the mean value and variance, and normalizing the image size; Step 4: Calculating ResNet-34 convolution lay output; 5, integrating that characteristics of the imitate eagle brain; 6, classifying that small targets; 7, training thesmall targetclassification network; 8, testing that small target classification network. The method of the invention can greatly reduce the parameters of the whole connection layer by using the global pooling operation, and the method of using the feature parallel fusion can simultaneously utilize the features of different levels to carry out the small target recognition, thereby achieving better classification effect.

The invention provides an elevator traction machine absorber based on metal rubber. The elevator traction machine absorber comprises a bearing panel, and is characterized in that first counter bores are formed in the four corners of the upper side face of the bearing panel, vibration attenuation machine feet are welded below the corresponding positions of the first counter bores, each vibration attenuation machine foot comprises a machine foot base and a metal sleeve, a through hole is formed in the middle portion of each machine foot base, each metal sleeve penetrates the corresponding through hole to be fixed to the corresponding machine foot base, and two or more layers of different-density metal rubber is arranged in each metal sleeve; and a base plate is fixedly arranged below the machine foot bases, three second counter bores are evenly distributed to the upper side face of each machine foot base, and the base plate and the machine foot bases are fixedly connected in the manner that first inside spread eagle bolt assemblies penetrate the second counter bores. The elevator traction machine absorber is simple in structure and convenient to operate, the defect that elastic materials of an existing traction machine absorber are difficult to achieve long-term stable work under the high temperature condition is overcome, and the requirement of a vibration attenuation supportingdevice for preventing side turning of the traction machine is met.

A precision micro taxidermy cutting instrument is provided which includes a single hold tailless frame member to which a pivotable body member is attached. The body member can provide a blade element which is concave and through which a lifting edge which cuts and a retention edge which cuts can both combine to provide a centering feature. This centering feature can be enhanced by blade body designs which also center through the use of an offset concave element. A chisel element can separate the hide from the viscera and through the selection of appropriate angles and the like, a continuous and precise cut can be made in taxidermy operations. The frame member can include a thumb rest as well as a rotational restraining element which are diametrically opposed and which can present roughly orthogonal forces. Flared separators can be included on the blade body member to further enhance the operation of the device. The open design, trademark named the "Flying Eagle(TM)" feature is also provided through a variety of releasable locking mechanisms which permit pivoting and locking in place at various locations.

The invention relates to an eagle policy and adaptive NM simplex-based photovoltaic model parameter identification method. The method comprises the following steps of S1: obtaining a photovoltaic model by adopting a photovoltaic IV curve test instrument to perform an IV characteristic curve, and setting a range of photovoltaic model parameters according to the number of serial and parallel solar cells of a photovoltaic array; S2: performing a rough global search on the photovoltaic model parameters by adopting a swarm intelligent optimization algorithm, and obtaining a plurality of relatively good photovoltaic model parameter initial value vectors; S3: performing a further rough local search on a group of optimal photovoltaic model parameter vector accessories in the step S2 by adopting a plurality of Nelder-Mead simplexes; and S4: performing a further fine local search on optimal model parameter vectors in the step S3 by adopting a single adaptive Nelder-Mead simplex to obtain optimal photovoltaic model parameter vectors. According to the technical scheme, the precision, speed and reliability of photovoltaic model parameter identification can be remarkably improved.

The invention discloses tissue preserving fluid. Each 50mL of the tissue preserving fluid is prepared from the following components: 50ml of DMEM (Dulbecco's Modified Eagle Medium) / F12, 90 to 110mu Lof bFGF (Basic Fibroblast Growth Factor), 240 to 260mu L of N2, 480 to 520mu L of B27, 40 to 60mu L of Y-27632, 400 to 500ng of BDNF (Brain Derived Neurotrophic Factor) and 400 to 500ng of NT3. By applying the tissue preserving fluid disclosed by the invention, the activity of fresh tumor cells can be kept for relatively long time, so that the efficiency of obtaining a PDX (Patient-Derived tumor Xenograft) model is improved. Compared with convectional preserving fluid, the PDX model utilizing the preserving fluid has the advantage that the tumor formation rate is greatly increased and the tumor utilization rate is greatly improved; the tumor formation and primary cell culture time is remarkably shortened. Moreover, the method disclosed by the invention can be used for enhancing the activity of a chordoma sample, so that the cost of a chordoma PDX model experiment is reduced; precision medical treatment can be carried out on tumors of more patients through the PDX model, so that the possibility of healing chordoma is improved.

The invention discloses a high-altitude bird repeller for a power iron tower. The high-altitude bird repeller comprises a fixing frame, a power windmill and a simulated bird, wherein the fixing frame comprises a base and a supporting rod; a fixing hole is formed in the base; and one end of the supporting rod is connected with the base, and the other end of the supporting rod is connected with the simulated bird. Fan blades drive a fan to mechanically charge a storage battery; and meanwhile, a movable rod drives wings to flutter, a storage battery supplies power to a sound production device and a light emitting device, the sound production device can produce a loud eagle sound, and the light emitting device can emit flickering light, so that the aim of effectively repelling birds is fulfilled. The high-altitude bird repeller for the power iron tower is simple in structure, economical and practical.

The invention provides an eagle claw type automatic Chinese chestnut opening machine which comprises a frame. A feeding system, a cutting system and a driving system are arranged on the frame, the feeding system comprises a feeding box and a conveying belt, a plurality of elastic Chinese chestnut fixing claws are arranged on the conveying belt, and anti-slip projections are arranged on the Chinese chestnut fixing claws. Each Chinese chestnut can be accurately positioned and firmly fixed and then cut by a saw blade wheel, the height of the saw blade wheel can be automatically adjusted according to the shape and the size of each Chinese chestnut, and the notch effect is fine after the Chinese chestnuts are cut. In addition, the eagle claw type automatic Chinese chestnut opening machine has the advantages of reasonable structural design, simple and effective process, high cutting speed, convenience in maintenance and durability.

The present invention relates to the field of marine biota specimen preparation technology, in the concrete, it relates to a method for making ray or eagle yay fish into specimen. Said method includes the following several steps: opening middle seam on sample fish abdomen, dressing, soaking the sample fish in alcohol, using arsenic trioxide to make treatment, making wooden internal model, shaping, forming and naturally drying by airing.

The invention provides a serum-free culture medium system for culture of primary human epidermic cells, relates to the technical field of biological medical materials, and discloses application of a serum-free culture medium in in-vitro culture of human epidermic cells. The serum-free culture medium system has the advantage that the cholera toxin and various anchoring factors are not added, the potential hidden hazard due to complicated components is avoided, the epidermic cells can well grow, and the requirement of scaled production and application is met. The serum-free culture medium system for culture of primary human epidermic cells comprises a basic culture medium and exogenous adding factors, wherein the basic culture medium is formed by mixing a DMEM (dulbecco's modified eagle medium) commercial culture medium and a F12 commercial culture medium according to a certain ratio, and can also adopt a commercialized cell culture medium MCDB153; the exogenous adding factors comprise L-glutamine, EGF (epidermic growth factors), BPE (bovine pituitary extract), insulin, penicillin-streptomycin, calcium chloride, transferrin, adenine, hydrocortisone, and sodium selenite.

The invention discloses a cell culture medium for preparing a human skin flbroblast sheet. The cell culture medium comprises the following components: (1) a basic culture medium, namely, H-DMEM (high glucose dulbecco's modified eagle medium) or DMEM-F12; (2) an additive component, namely, vitamin C 50-100 [mu]g / ml, and epidermal growth factors (EGF) 3-20 ng / ml, and (3) serum 5-15%. In the fibroblast basic culture medium, other components are added, so that the cultured fibroblasts form a sheet. The invention further discloses a using method of the cell culture medium.

The invention discloses a megawatt wind power generation blade with a blade-tip turbulent flow structure. The root of the blade is connected with a wind power generator, the leeward of the blade is a suction surface while the windward side is a pressure surface, the turbulent flow structure formed by a lightening-protection flash receiving point and a fusion transition portion and used for improving pneumatic performance of a blade tip is arranged at the position close to one end of the blade, the fusion transition portion bends to the position of the suction surface, and the lightening-protection flash receiving point is fixed on the suction surface of the fusion transition portion. The blade-tip turbulent flow structure is designed by the application of the bionics concept and reference of large birds such as eagles gliding and flying to have feathers at the positions of wing tips bend upwards as well as successful application of winglets at aircraft wingtips which is used for reference, induced resistance can be effectively decreased, blade tip turbulence can be restrained, pneumatic noise of the blade can be effectively controlled, and pneumatic performance of the blade can be improved.

The invention belongs to the technical field of building construction, and relates to a molding method of a window opening. A beam bottom molding plate is braced on the bottom of a structural beam firstly, a first batten is additionally arranged on the inner side of the beam bottom molding plate corresponding to a window, beam side molding plates are symmetrically braced on the two sides of the structural beam, sill lintel side molding plates are symmetrically braced on the two sides of a sill lintel, a second batten is fixedly braced at a window bottom outer port, the window opening is pouring-molded along with a concrete structure, after the concrete structure is poured, a braced window top opening molding plate and a window bottom opening molding plate are disassembled, an opening which is increased in height from outside to inside and comprises an eagle beak is formed in the upper port of a window cavity, and the opening which is increased in height from outside to inside and comprises a water slope is formed in a lower port of the window cavity, so that the once molding of the window opening is achieved. The molding method is simple in method, convenient to operate, high in production efficiency, low in cost, and capable of effectively solving the window leakage problem and can be widely applied to the molding processes of the window openings during building construction.

The invention discloses a tooth-care soft candy and a preparation method thereof. The tooth-care soft candy is composed of the following raw materials in parts by weight: 1.5-2.5 parts of eagle tea, 2.0-3.0 parts of dried hawthorn, 10-12 parts of gelatin, 25-32 parts of white sugar, 0.012-0.018 part of vitamin A and 0.001-0.0014 part of vitamin D. The invention further discloses a preparation method of the tooth-care soft candy. The prepared soft candy has crystal clear appearance, eagle tea flavor and no any food additives, and is sour and sweet and delicious, thus being a visually appealing and flavorous green tooth-care healthcare good.

The invention relates to a culture medium for culturing a Marc-145 cell. The culture medium consists of the following components: DMEM (dulbecco's modified eagle medium), an enzymolysis gelatin solution, amino acid DMEM solution and fetal calf serum in a volume ratio of (1-2):(1-5):(2-5):100. The invention further provides a preparation method of the culture medium, wherein the preparation method comprises the following steps: respectively weighing the DMEM, the enzymolysis gelatin solution, the amino acid DMEM solution and the fetal calf serum according to the volume ratio of a formula, and mixing to obtain the culture medium. The culture medium for culturing the Marc-145 cell disclosed by the invention can greatly reduce usage amount of the bovine serum in a Marc-145 cell culture process, so that the production cost is reduced; meanwhile, the preparation method is simple and easy to operate.

The invention discloses a mesenchymal stem cell cryopreservation agent which comprises 0.2%w / v cane sugar, 0.5%w / v low-density lipoprotein, 3%w / v trehalose, 2%w / v lecithin, 100ppm w / v polypeptide, 1.5%w / v bFGF, 1%w / v vitamin E, 5% v / v glycerin and the balance of a DMEM (Dulbecco's Modified Eagle Medium) and fetal calf serum in a ratio of 1:1 till 100ml. In the mesenchymal stem cell cryopreservation agent disclosed by the invention, the glycerin, the trehalose, the vitamin E and the polypeptide have definite functions of preventing external damage to cells, the polypeptide also has a function of preventing cells from damage of ice crystal generated in cryopreservation, and the other components are essential for maintaining survival of specific MSCs (Mesenchymal Stem Cells). The cell cryopreservation agent disclosed by the invention is applied to cryopreservation and culture of cells, is particularly capable of increasing the survival rate of revived cryopreservation cells, and has relatively good application prospects.

The invention relates to a frozen stock solution used for preserving adult stem cells for a long time, and a preparation method thereof. Every 100ml of base frozen stock solution contains: 10-20ng / ml of herba epimedii total flavones, and every 100ml of base frozen stock solution contains: 50-70% of DMEM (dulbecc' modified eagle medium) cluture medium, 10% of dimethyl sulfoxide, and 40-20% of Knock-out serum. The frozen stock solution can improve capacities for preventing cells from frozen damage and resisting oxidation and apoptosis after the cells are recovered, prevents the cells from contacting with heterologous animal original serum during a frozen stock process and further increases security of the adult stem cells for clinic applications.

The invention relates to a human mycoplasma pneumoniae culture medium and a diagnosis kit thereof. The human mycoplasma pneumoniae culture medium comprises a TSB culture medium and an additive, wherein the additive comprises bovine serum, DMEM (dulbecco's modified eagle medium), avian yolk extract, sodium pyruvate and glutamine. The DMEM accounts for 5-15 vol% of the human mycoplasma pneumoniae culture medium. On the basis of the total volume of the human mycoplasma pneumoniae culture medium, the avian yolk extract content is 10-100 ml / L, the sodium pyruvate content is 0.2-1.0 g / L, and the glutamine content is 0.3-1.0 g / L. Compared with the prior art, the culture medium provided by the invention performs practical innovation design on the basis of abundant practices, conforms to the demands, ensures the specificity and reliability of the clinical Mp infection diagnosis, is convenient to use, and has the advantages of high speed, high safety, environment friendliness and resource saving.