218 results about "Eagle" patented technology

A composition for cryopreservation of cells and tissues of human and other animals in a safe manner without using toxic substances such as DMSO, as well as for freeze preserving or freeze-drying of foods and pharmaceuticals. In examples, ε-poly-L-lysine is reacted with succinic anhydride so that 60% or more of amino groups are blocked; and, thus obtained polymer compound is added to Dulbecco-modified eagle MEM culture medium (DMEM) on market sale to form a cryopreservation liquid. In examples for foods or pharmaceuticals, the ε-poly-L-lysine derivative was added by 0.5-10 wt % to curb freeze concentration.

The invention discloses an electric-gas compound driving flexible finger eagle-claw-imitating logistics packaging manipulator comprising a driving part and four same finger parts. Each finger part comprises a finger root joint, a middle finger joint and a fingertip joint. According to the manipulator, the acting point of contact force and the direction of acting force can be selected according toconditions, thus an object is not deformed or damaged in the grabbing process, and grabbing is more accurate and reliable. Meanwhile the angles of the finger root joints and the grabbed object are adjusted, can adapt to large change of the size of the grabbed object and adapt to the change of the shape and vertical and transverse placing of the grabbed object, the acting point and magnitude of thegrabbing force have flexible self-adaptability, response is fast, and buffering performance is good.

The invention discloses a method for sequential culture of human umbilical cord blood mesenchymal stem cells by using two culture media. A lymphocyte separation medium density gradient centrifugation method is used for separating umbilical cord blood mononuclear cells, a dulbecco modified eagle medium (DMEM)/F12 is used for primary culture of the mesenchymal stem cells of the human umbilical cord blood, and the method increases adherence of the cells, reduces growth of osteoclast like cells remarkably, facilitates formation of human umbilical cord blood-mesenchymal stem cells (hUCB-MSCs) colonies, and greatly improves successful rate of culture of the hUCB-MSCs. The DMEM/F12 is used continuously for subculturing to a P2 generation, the method is combined by a using enzymatic digestion and differential velocity adherent method simultaneously, and the method facilitates purification of P1-P2 generation cells remarkably. A P3 generation and the following generations are cultured by using an Oricell human umbilical cord mesenchymal stem cell culture medium, marker proteins and good morphological characteristics and growth characteristics of the hUCB-MSCs are maintained, and multilineage differentiation potential of the hUCB-MSCs is maintained. In addition, costs of the culture media adopted by the method are reduced remarkably.

The invention discloses a quantum-dot-based method for carrying out in-situ and real-time detection on heavy metal ions in cells. The method comprises the following steps of: A. preparing a carboxymethyl chitosan-CdTe quantum dot solution with certain concentration; B. digesting canine kidney cells overgrowing in a T25 cell culture flask bottle into a unicellular suspension, inoculating into a cell culture dish, and culturing; C. adding the carboxymethyl chitosan-CdTe quantum dot solution into the culture dish, and hatching for a period of time, thereby obtaining the canine kidney cells marked with fluorescence; and D. adding a DMEM (dulbecco's modified eagle medium) nutrient solution containing heavy metal ions with gradient concentration and unknown concentration respectively in washed cells in the culture dish, hatching together with the cells, detecting the average fluorescent intensity of the cells respectively by a flow cytometry, and drawing a standard curve through the changes of the cell fluorescent intensity, so that the concentration of the heavy metal ions in the cells can be calculated. The method is simple and convenient to operate; the fluorescence of marked cells can be quenched regularly as the increase of heavy metal ions, and the real-time and in-situ detection on the outer source heavy metal ions in the cells is realized.

The invention discloses a method and a special culture medium for subculturing chicken embryonic stem cells for a long time. The method is characterized by comprising the following steps of: isolating cells of area pellucida of X-stage blastoderm, dispersing into single cells or small cell masses through mechanical blowing and beating, inoculating on an STO feeder layer, culturing the chicken embryonic stem cells in the special culture medium at the temperature of between 37 and 38 DEG C, and subculturing once every 3 to 5 days, wherein the special culture medium comprises 600 to 900mL of conditioned medium, 50 to 150mL of fetal calf serum, 5 to 20mL of chicken serum, 5 to 25mL of one or a mixture of more of non-essential amino acids, 0.146 to 0.292g of L-glutamine, 6 to 14mu L of beta-mercaptoethanol, 1 to 2*10<6> IU of mouse leukemia inhibitory factor, 10 to 50mu g of alkaline fibroblastic growth factor and 5 to 20mu g of stem cell growth factor; and fixing the volume of the ingredients to 1,000mL by using a dulbecco's modified eagle medium (DMEM) (high glucose), and regulating the pH value to 7.2 to 7.5. The conditioned medium is obtained by the steps of: culturing BRL-3A cells until the cells are converged, collecting supernatant of the cultured cells, performing centrifugal separation, and filtering by using a filter membrane to obtain the conditioned medium. Compared with the prior art, the method has the advantages that: the long-term subculture of the chicken embryonic stem cells can be realized, the proliferation of the cells is quick, and the cloning yield is high.

The invention relates to a glede tea beverage and a preparation method thereof. According to the preparation method, glede tea, which accounts for 0.6 to 0.8 percent of the total weight of the beverage, is crushed into tea powder, sieved by a 50-mesh sieve and then weighed. After that, water accounting for 8 to 10 times of the weight of the glede tea powders is added into an extraction tank, heated to 75 to 90 DEG C at the room temperature, added with the tea powders for stirring extraction for 8 to 12 minutes. Then the materials are filtered by a filter screen being 200 to 300 meshes to obtain filter liquid (1). Water accounting for 4 to 6 times of the weight of the filter residue is adopted for extracting for 4 to 6 minutes under the same conditions of the filter residue. The filtering operation is carried out by the filter screen being 200 to 300 meshes to obtain filter liquid (2). The filter liquids (1) and (2) are merged and added into the extraction tank. The filter liquid is heated to 60 to 80 DEG C and the pressure thereof is reduced the to -15kPa to 20kPa for concentration. Condensed liquid, i.e. aromatic water, is collected. The collecting of the condensed liquid is stopped until the weight of the condensed liquid is 1 to 1.5 times of that of the tea powder. Then concentration is continuously carryed out until the weight is 1/4 to 1/3 of the total filter liquid weight. The spray drying is carryed out to obtain dry powder. The prepared dry powder, the aromatic water, the white granulated sugar and the citric acid are mixed and prepared. The glede tea beverage has the advantages that the tea beverage has the effects of relieving summer heat and quenching the thirst, helping digestion and enhancing the human body immunity, the quality is easy to control, and in addition, the taste is good.

The invention relates to a construction method of a fin cell line of anabarilius grahami and belongs to the technical field of freshwater aquatic organism cell culture. The method comprises that the anabarilius grahami tail fin tissue cell serves as a material, a tissue block method is adopted, and the primary culture is strated, culturing is achieved in a dulbecco modified eagle medium (DMEM)/F12 containing fetal calf serum and human basic fibroblast growth factors and having a PH value ranging between 7.0 and 7.4; and subculture is performed by using a trypsin digestion method. The method has the advantages that 1, the minimally invasive processing is adopted, on the premise that the fish is alive, and the operation is easy and feasible; 2, the anabarilius grahami fin muscle cell line (AGF) is in a fibroblast cell patternin a fiber structure, continuous passage can be achieved, the line can be applied to biological characteristic research directly, and requirement for anabarilius grahami molecular cell level theoretical research is met; and 3, the construction method is also applicable to cell lines of constructed fin rays of other fishes.

The invention discloses a human pluripotent stem cell culture medium and a culture method of human pluripotent stem cells. The human pluripotent stem cell culture medium comprises a basic culture medium and additives, wherein the basic culture medium is a DMEM/F12 (Dulbecco Modified Eagle Medium); the additives comprise 400-600mu g/ml sodium bicarbonate, 8-20ng/ml sodium selenate, 10-30ng/ml recombinant human insulin growth factor, 80-120ng/ml recombinant human alkali fibroblast growth factor, 7-13mu g/ml recombinant human lactoferrin, 50-70mu g/ml ascorbic acid and 1-3ng/ml recombinant human transforming growth factor. The culture medium disclosed by the invention is definite in component, stable in quality and free of animal resource components, in the culture process, no animal source cells are needed to be used as a feeder layer, cultured hES cells and hips cells are less in possibility of having human rejection reaction after being transplanted, and the growth efficiency is high.

The invention discloses a resNet-based small target recognition method for imitating eagle brain feature integration: step 1, establishing a small target recognition map library; 2, initializing thatsetting; 3, normalize images: calculate that mean value and variance of all images in the small target recognition map library, normalizing all images by use the mean value and variance, and normalizing the image size; Step 4: Calculating ResNet-34 convolution lay output; 5, integrating that characteristics of the imitate eagle brain; 6, classifying that small targets; 7, training thesmall targetclassification network; 8, testing that small target classification network. The method of the invention can greatly reduce the parameters of the whole connection layer by using the global pooling operation, and the method of using the feature parallel fusion can simultaneously utilize the features of different levels to carry out the small target recognition, thereby achieving better classification effect.

The invention provides an elevator traction machine absorber based on metal rubber. The elevator traction machine absorber comprises a bearing panel, and is characterized in that first counter bores are formed in the four corners of the upper side face of the bearing panel, vibration attenuation machine feet are welded below the corresponding positions of the first counter bores, each vibration attenuation machine foot comprises a machine foot base and a metal sleeve, a through hole is formed in the middle portion of each machine foot base, each metal sleeve penetrates the corresponding through hole to be fixed to the corresponding machine foot base, and two or more layers of different-density metal rubber is arranged in each metal sleeve; and a base plate is fixedly arranged below the machine foot bases, three second counter bores are evenly distributed to the upper side face of each machine foot base, and the base plate and the machine foot bases are fixedly connected in the manner that first inside spread eagle bolt assemblies penetrate the second counter bores. The elevator traction machine absorber is simple in structure and convenient to operate, the defect that elastic materials of an existing traction machine absorber are difficult to achieve long-term stable work under the high temperature condition is overcome, and the requirement of a vibration attenuation supportingdevice for preventing side turning of the traction machine is met.

The invention relates to an eagle policy and adaptive NM simplex-based photovoltaic model parameter identification method. The method comprises the following steps of S1: obtaining a photovoltaic model by adopting a photovoltaic IV curve test instrument to perform an IV characteristic curve, and setting a range of photovoltaic model parameters according to the number of serial and parallel solar cells of a photovoltaic array; S2: performing a rough global search on the photovoltaic model parameters by adopting a swarm intelligent optimization algorithm, and obtaining a plurality of relatively good photovoltaic model parameter initial value vectors; S3: performing a further rough local search on a group of optimal photovoltaic model parameter vector accessories in the step S2 by adopting a plurality of Nelder-Mead simplexes; and S4: performing a further fine local search on optimal model parameter vectors in the step S3 by adopting a single adaptive Nelder-Mead simplex to obtain optimal photovoltaic model parameter vectors. According to the technical scheme, the precision, speed and reliability of photovoltaic model parameter identification can be remarkably improved.

The present invention relates to the field of marine biota specimen preparation technology, in the concrete, it relates to a method for making ray or eagle yay fish into specimen. Said method includes the following several steps: opening middle seam on sample fish abdomen, dressing, soaking the sample fish in alcohol, using arsenic trioxide to make treatment, making wooden internal model, shaping, forming and naturally drying by airing.

The invention provides a serum-free culture medium system for culture of primary human epidermic cells, relates to the technical field of biological medical materials, and discloses application of a serum-free culture medium in in-vitro culture of human epidermic cells. The serum-free culture medium system has the advantage that the cholera toxin and various anchoring factors are not added, the potential hidden hazard due to complicated components is avoided, the epidermic cells can well grow, and the requirement of scaled production and application is met. The serum-free culture medium system for culture of primary human epidermic cells comprises a basic culture medium and exogenous adding factors, wherein the basic culture medium is formed by mixing a DMEM (dulbecco's modified eagle medium) commercial culture medium and a F12 commercial culture medium according to a certain ratio, and can also adopt a commercialized cell culture medium MCDB153; the exogenous adding factors comprise L-glutamine, EGF (epidermic growth factors), BPE (bovine pituitary extract), insulin, penicillin-streptomycin, calcium chloride, transferrin, adenine, hydrocortisone, and sodium selenite.