371results about How to "Does not affect normal growth" patented technology

The invention discloses a mycotoxin detoxification agent for feed and a preparation method and a feed additive thereof, and belongs to the field of feed additives. The mycotoxin detoxification agent can be used for detoxification of feed raw materials and compound feed of mycotoxin polluted corn, grains and the like. The mycotoxin detoxification agent consists of modified montmorillonite, yeast cell wall, chitosan and the like; and the preparation method has simple process. The novel mycotoxin detoxification agent for feed can effectively remove pollution of multiple mycotoxins such as aflatoxin, zearalenone, T2 toxin, vomitoxin and the like in the feed raw materials and the feed of the corn, the grains and the like, and prevent various diseases of livestock and poultry because of eating the mycotoxin polluted feed. The mycotoxin detoxification agent has simple preparation process, stable and controllable quality, convenience in use and low price, meanwhile has the advantages of supplementing nutrients, improving the disease resistance of cultured animal bodies and avoiding adsorbing nutrient elements in the feed, and guarantees the health and safety of the cultured animals.

The invention discloses a method for controlling weeds in a tea garden, which comprises the following steps: first, plowing is carried out on the ground between tea plant rows in winter to remove root systems of the weeds such as nutgrass flatsedge, alligator alternanthera, greenbrier, chinaroot greenbrier, cogongrass, smartweed, lygodium and the like; a high-quality organic fertilizer is applied in spring: a certain amount of rape seed cake fertilizer is broadcast; the ground is covered with rice straws in spring: after the rape seed cake fertilizer is broadcast, all tea plant rows in the garden are immediately evenly covered with a certain amount of the rice straws; the high-quality organic fertilizer is applied in summer: a proper amount of the rape seed cake fertilizer is broadcast at a certain time; and the ground is covered with the rice straws in summer: after the rape seed cake fertilizer is broadcast, all tea plant rows are immediately evenly covered with a certain amount of the rice straws. The method enriches biodiversity of the tea garden, improves the ecological condition of the tea garden, increases tea yield, improves the quality of tea leaves; in addition, the method has the advantage of simple operation, is easy to be accepted by tea growers and has great popularization and application prospect.

The invention provides an ecological method for restoring polycyclic aromatic hydrocarbon polluted farmland soil, which comprises the following steps: selecting plants such as corns, soybean, castor-oil plants and alfalfa with the restoring function of the polycyclic aromatic hydrocarbon polluted soil for seeding according to a plant conventional mode, wherein the plant growth period is one growth season and is between 100 and 150 days; 2, applying efficient biological microbial agents to farmland at 5 to 10 days before the plant seeding with the application amount between 40 to 50 kg/mu, and carrying out ploughed fallow on the farmland in 24 hours after the application; 3, applying sludge fermentation fertilizer into the farmland at 2 to 3 days before the plant seeding with the application amount of 100 to 200 kg/mu, and carrying out ploughed fallow and ridge preparation on the land in 24 hours after the application; and 4, installing a water discharge pump used for water discharge in a rain reason in the restoring field every 4 to 5 ridges of the land, and carrying out ploughed fallow for 2 to 3 times in each month of the three months before the plant growth. The invention has the characteristics of low cost, strong operability, no secondary pollution and high restoring efficiency.

The invention discloses a visual inspection system and method for scale micro changes of plant fruits. The visual inspection system comprises an image collection device, an image processing and controlling device, a supporting device and an external trigger device. After images of the plant fruits are collected, denoised, computed and converted into binary images, the diameters of the fruits are obtained. By means of the system, the scale micro changes of the plant fruits can be detected in a non-contact and high-accuracy mode, and normal growth of the scale micro changes of the plant fruits will not be affected. The visual inspection system and method for the scale micro changes of the plant fruits can be widely applied to the aspects such as searching of plant growth rules, growth abnormity detection, water-saving irrigation and production optimum control.

A method based on the computer visualization for detecting the information about the water demand of crops in order to save water in intelligent irrigation system is disclosed. Its system is composed of a black reference with known sizes, image acquisition equipment, image acquisition card and computer. Said method includes such steps as arranging said block reference close to stem or fruit (seed) of crops, taking the image, filtering noise, separating the reference and object to be detected, calculating the numbers of their pixels, and calculating the water demand.

The invention discloses a quantum-dot-based method for carrying out in-situ and real-time detection on heavy metal ions in cells. The method comprises the following steps of: A. preparing a carboxymethyl chitosan-CdTe quantum dot solution with certain concentration; B. digesting canine kidney cells overgrowing in a T25 cell culture flask bottle into a unicellular suspension, inoculating into a cell culture dish, and culturing; C. adding the carboxymethyl chitosan-CdTe quantum dot solution into the culture dish, and hatching for a period of time, thereby obtaining the canine kidney cells marked with fluorescence; and D. adding a DMEM (dulbecco's modified eagle medium) nutrient solution containing heavy metal ions with gradient concentration and unknown concentration respectively in washed cells in the culture dish, hatching together with the cells, detecting the average fluorescent intensity of the cells respectively by a flow cytometry, and drawing a standard curve through the changes of the cell fluorescent intensity, so that the concentration of the heavy metal ions in the cells can be calculated. The method is simple and convenient to operate; the fluorescence of marked cells can be quenched regularly as the increase of heavy metal ions, and the real-time and in-situ detection on the outer source heavy metal ions in the cells is realized.

The invention discloses a method for quickly and preliminarily screening excellent tea tree resources based on contained contents. The method comprises the following steps: taking first expanded leaves of tea tree buds with consistent growth conditions and growth vigor, performing freezing treatment to the leaves by liquid nitrogen, crushing the leaves, using methanol to quickly and directly extract the leaves, centrifuging the leaves, taking supernatant, utilizing a high performance liquid chromatography device to detect the contained contents in the tea leaves, comparing the contents of the contained ingredients, preliminarily screening the excellent tea tree genetic resources, and based on the preliminary screening, adopting the known method to further screen the preliminarily screened excellent tea tree genetic resources. The method is simple and convenient, low in the device requirements, low in the reagent dosage, simplifies the pretreatment procedures of the test materials, greatly reduces the labor intensity, reduces the determination cost and time, and can be used for high throughput analysis.

The invention belongs to the technical field of plant cultivation, provides a plant cultivation transplant technology, and relates to an ascendens mucronatum cuttage technology, in particular to the ascendens mucronatum cuttage cultivation and transplant technology. The ascendens mucronatum cuttage technology includes the steps of preparing a seedbed, picking ears, conducting root promoting treatment, conducting cuttage, managing and protecting the seedbed, hardening seedlings in a greenhouse and the like, wherein the seedbed is managed and protected under the specific temperature, humidity and light conditions, a proper amount of fertilizer and pesticide for preventing and controlling diseases and pests are applied before and after cuttage for ascendens mucronatum seedling cultivation. By means of the ascendens mucronatum cuttage technology, the ear picking cost is lowered, the ears are wide in source, the seedbed management and protection costs are lowered, the seedbed can be reused in the same year, the seedbed is used for cuttage in spring, survival ear seedlings can be transplanted outside the greenhouse to be hardened when summer comes, the seedbed is idle, cuttage in summer is facilitated, and the use frequency of the seedbed is increased.

The invention discloses a method for controlling large algae in a sea cucumber pond on the basis of biotechnology. After water temperature rises to 5 DEG C for the first time after early spring and when the large algae grow in the pond or pond transparency is larger than 80cm, bacillus subtilis is evenly sprinkled to the water surface of the pond with the large algae, the microbial content per square meter is 1.5-2.5 billion, diatom breeding fertilizer and fermentation organic fertilizer for promoting bacillus subtilis growth and lowering transparency are evenly sprinkled, so that the pond transparency is lowered to 80cm, and restraint of the large algae is achieved. According to the biological method, the relative transparency is adjusted through alga introduction and matched using of specific inorganic fertilizer, organic fertilizer and bacillus subtilis according to growth laws of the large algae in ponds of different areas at different time nodes so as to restrain photosynthesis of the large algae, and accordingly the goals of controlling rampant growth of the algae and not influencing normal growth of sea cucumbers are achieved.

The invention relates to a plant restoration method of tetracycline-polluted wastewater, which comprises the following steps: selecting flat foam plastic as a floating bed, making holes which are uniformly distributed in the floating bed, and growing 1-2 ciliate desert-grass plants in each hole, wherein the distance between every two adjacent holes is 10-15 cm; discharging tetracycline-polluted wastewater to be treated into a water storage tank; putting the floating bed in the water storage tank, wherein the root of the ciliate desert-grass is completely immersed in water, and the overground part of the ciliate desert-grass is above the water; and finishing water body restoration within 4-6 days. The invention does not have special requirements for the pH value of the polluted water body, has the advantages of high tetracycline removal efficiency, low investment, low maintenance cost, small engineering work load, low energy consumption and the like, is simple to operate, can be used repeatedly, does not generate secondary pollution, and is a green restoration technique with wide application range and favorable effect.

The invention provides a culture water quality regulating and controlling method of a penaeus vannamei boone freshwater pond. The culture water quality regulating and controlling method comprises the following steps in sequence: (1) carrying out anaerobic nitrification; (2) carrying out aerobic nitrification; (3) purifying photosynthetic bacteria; (4) carrying out microalgae cultivation and purification. Water pumped out from the freshwater pond for culturing penaeus vannamei boone is subjected to the anaerobic nitrification, the aerobic nitrification, the photosynthetic bacterium purification and the microalgae cultivation and purification in sequence, and then the water is reflowed back to the freshwater pond for culturing penaeus vannamei boone. According to the culture water quality regulating and controlling method, organic matters in the pond water can be decomposed into small molecular substances and the small molecular substances are cultured and absorbed by the photosynthetic bacteria and mixotrophic microalgae, and are converted into a microalgae organism; the treated and qualified water and cultured beneficial microalgae are discharged into the pond for cyclic utilization; after the beneficial microalgae enter the pond, population advantage is formed and a culture biological environment is improved; the water quality treatment effect is good, and a whole water treatment process does not utilize a culture water body and normal growth of the penaeus vannamei boone is not influenced; the method is high in operability and low in operation cost, and is safe and reliable to the cultured penaeus vannamei boone.

The invention provides special weedicide for bletilla striata and application of the special weedicide and belongs to the field of plant cultivation. The weedicide is prepared from an effective component and an auxiliary agent, wherein the content of the effective component accounts for 55 percent to 65 percent of the total amount of the weedicide and the pH (Potential of Hydrogen) value of the weedicide is 5.5 to 6.5; the effective component is prepared from the following components in parts by weight: 18 to 24 parts of jatrorrhizine, 15 to 20 parts of palmatine, 6 to 8 parts of benzothiazooxadiazon, and 6 to 8 parts of flucetosulfuron. The weedicide provided by the invention can be used for effectively preventing and removing almost annual grass weeds, broadleaf weeds and the like in bletilla striata fields, has the advantages of wide weeding spectrum and good prevention and treatment effect and is relatively moderate to bletilla striata plants; when the weeds are removed, normal growth of the bletilla striata plants is not influenced. When the weedicide is used for removing the weeds of the bletilla striata fields, the weeding effect is good and no phytotoxicity on the bletilla striata plants is caused; meanwhile, active components of the weedicide are natural small molecule compounds so that pesticide residues and environment pollution are not caused.

The invention provides a method for quickly building a genetic transformation regeneration system of wine grape Riesling, and belongs to the technical field of transgenic breeding of grapes. The method is characterized by comprising the following steps: performing tissue culture to induce grape anther to obtain callus; subculturing and screening to obtain embryonic callus; with embryonic callus as a receptor, screening through a selective medium by an agrobacterium tumefaciens-mediated method to obtain resistant callus; and then regenerating a grape plant. Whether the gene gfp is expressed can be observed and reported by PCR (Polymerase Chain Reaction) verification and through a stereo fluorescence microscope, and a transformant also can be identified quickly, therefore, the grape transgenosis efficiency can be improved. Compared with the prior art, the method has the advantages that the culture medium has a good effect on culturing and screening, and achieves quick regeneration of a Riesling transgenic grape plant, thus the grape transgenosis efficiency is greatly improved, and the regeneration rate reaches 81%; the transformation cycle is short, the transgenic plant can be obtained within seven months; the transformant is greatly increased; the transformation rate can reach 63%.

The invention discloses a breeding method for chickens. One month after roasters are castrated serves as a recovery stage, and recovery stage feed is adopted for feeding the castrated roasters, wherein the recovery stage feed is prepared from, by weight, 40-60 parts of wheat, 30-50 parts of sorghum, 15-30 parts of chaff, 15-30 parts of millet, 10-20 parts of dried small shrimps, 10-20 parts of fish meal, 10-25 parts of bone meal, 5-15 parts of eggshell powder, 5-10 parts of red date powder, 5-10 parts of edible fungus powder, 2-5 parts of salt, 0.5-1 part of pearl powder, 0.5-1 part of selenium yeast, 15-20 parts of kiwi fruits, 0.1-0.5 part of vitamin K and 3-6 parts of traditional Chinese medicine powder. By feeding the castrated roasters with the recovery stage feed in the recovery stage and carrying out scientific management, the wounds of the castrated roasters heal fast and are not likely to be infected, and the survival rate of the castrated roasters is remarkably increased; besides, the normal growth of the castrated roasters is not influenced, the castrated roasters can grow fast, and the anti-stress capability of the castrated roasters is improved.