49 results about "Ictalurus" patented technology

The present invention discloses one kind of fast frozen white delicious fish fillet of spotted forked-tail Leiocassis longirostris and its production process. The production process includes the steps of temporarily raising, killing to eliminate blood, slicing, eliminating skin, trimming, soaking to sterilize, loading into tray and quick freezing, grading, coating with ice, packing, etc. The production process features complete elimination of blood, low temperature of fish fillet in icy water at 0-4 deg.c, soaking fish fillet in ozone icy water and ultralow temperature quick freezing at below -35 deg.c. The production process has high fish fillet yield and less nutrient loss, and the produced fish fillet is white, translucent and delicious.

The invention discloses a Ictalurus punctatus compound feed, the contents of mass ratio of characteristic materials are as follows: vitamin A 0.005-0.007, vitamin D3 0.004-0.006, vitamin E 0.070-0.090, vitamin K3 0.002-0.004, vitamin B1 0.003-0.005, vitamin B2 0.005-0.007, vitamin B6 0.08-0.012, vitamin B12 0.001-0.003, nicotinamide 0.030-0.050, calcium pantothenate 0.10-0.30, folic acid 0.01-0.03, biotin 0.001-0.002, VC-phosphate 0.300-0.400, inositol 0.070-0.090, antioxidants 0.200-0.300, wheat-middlings 1.000-1.200, ferrous sulfate 0.400-0.600, copper sulfate 0.010-0.030, zinc sulfate 0.150-0.200, manganese sulfate 0.100-0.150, iodine 0.050-0.150, Se 0.050-0.070, cobalt chloride 0.010-0.030, magnesium sulfate 1.000-1.500, zeolite powder 5.0-6.0, xylo-oligosaccharide 0.008-0.012, bacillus licheniformis 0.01, weatings 90.-110., wheat-middlings 110-130, rice bran 75-85, soy bean powder 55-65, soybean meal 190-210, peanut meal 25-35, fish meal 95-105, cottonseed meal 75-85, rapeseed meal 110-130, gem meal 45-55, soybean oil 15-25, lysine 0.8-1.2, methionine 0.8-1.2, salt 2.0-2.5, betaine 3-5, allicin 0.1-0.3, choline chloride 1.0-2.0, calcium dihydrogen phosphate 15-25. Compared with other feeds, the usage rate of feed nutrient ingredients for Ictalurus punctatus is increased by 10-15%, feed coefficient is reduced by 5-10%, feed waste is reduced, nitrogen and phosphorus excretion of Ictalurus punctatus in the environment is reduced, at the same time the survival rate of Ictalurus punctatus is improved significantly.

The invention relates to a method for preparing bone paste by using Ictalurus punctatus bone, pertaining to the technical field of the comprehensive utilization of the Ictalurus punctatus. The method takes frost fishbone as a raw material to prepare the finished bone paste product through thawing, rinsing, draining, enzymolysis meat removing, smashing, grinding, packaging, sealing and sterilizing. The calcium content of the Ictalurus punctatus bone can reach 4150mg / 100g. The Ictalurus punctatus bone is utilized to produce the bone paste, thus increasing the processing added value and promoting the comprehensive utilization rate of freshwater fish.

The invention discloses a microsatellite family identification method for ictalurus punctatus. The microsatellite family identification method comprises the following steps: (1) breeding an ictalurus punctatus family by adopting an artificial propagation method, and carrying out polyculture on 100 family descendants in one pond; (2) analyzing gene types of an ictalurus punctatus breeding group on 18 microsatellite marker sites by adopting a fluorescence marking primer and a multi-PCR (Polymerase Chain Reaction) amplification method; screening 10 effective microsatellite markers which have high polymorphism and are suitable for family identification; (3) optimizing a multi-PCR amplification system of the 10 microsatellite markers and analyzing gene types of the polycultured descendants on 10 microsatellite marker sites; (4) identifying a parental origin of each descendant according to the gene types of parents and the descendants. According to the microsatellite family identification method disclosed by the invention, a paternity testing method is established for the ictalurus punctatus for the first time and the identification accuracy reaches 96.6 percent; polyculture individual parents of the ictalurus punctatus can be rapidly and accurately identified. The microsatellite family identification method disclosed by the invention can be used for rapidly and accurately carrying out family identification on the polyculture individual parents of the ictalurus punctatus, and the breeding effect is improved.

The invention relates to a hot and spicy sauce containing Ictalurus Punctatus fishbone, and a preparation method thereof, and belongs to the technical field of food production. The raw materials of the hot and spicy sauce comprise a fishbone spicy paste, Ciba chili, pickled pepper, soy, a broad bean sauce, fermented soya bean, white sesame, white granulated sugar, monosodium glutamate, ten-medicine Xiang and potassium sorbate. The preparation method concretely comprises the following steps: heating fragrance oil, adding the pickled pepper, stir-frying, dumping the soy, heating to boiling, adding the broad bean sauce, the Ciba chili, the fishbone spicy paste, the fermented soya bean, the white sesame, the white granulated sugar, the monosodium glutamate, the fishbone spicy paste and potassium sorbate, carrying out mixing frying while continuously stirring, adding a proper amount of purified water to form a sauce, canning to a bottle the sauce while hot, sterilizing, and cooling to obtain the hot and spicy sauce. The hot and spicy sauce containing Ictalurus Punctatus fishbone enriches the kind of Ictalurus Punctatus fishbone foods, solves the present situation of the only utilization of the Ictalurus Punctatus fishbone as a feed raw material, is in favor of the culturing popularization of the Ictalurus Punctatus fishbone, improves the added values of the hot and spicy sauce, and promotes the efficient comprehensive utilization of the Ictalurus Punctatus.

The invention discloses a method for preparing rinsed fish fillets from fillets of ictalurus punctatus Rafinesque. The method is characterized by sequentially comprising preprocessing fish flesh, soaking fish fillets, preparing frozen fish flesh, slicing frozen fish flesh, putting the frozen fish flesh in a slicing machine and then slicing into rinsed fish fillets according to the thickness of the rinsed fillets. By the method, on the one hand, a novel method is found for the deep processing of the fillets of ictalurus punctatus Rafinesque, and the added value of the product is improved; on the other hand, a novel delicacy is provided for the rinsed fillet market. When the fish flesh prepared by the method is sliced and rinsed, the fish flesh is not loosened and broken, and the elastic property is good.

The present invention discloses a manufacturing method of a soft canned seasoned and instant ictalurus punctatus which includes the following steps: pre-treating freshwater fish, removing fishy smell and pickling, drying, oil-frying, seasoning and packaging, and sterilizing and cooling. The soft canned seasoned and instant ictalurus punctatus uses freshwater fish with relatively high fat content as raw materials, the same technology can be popularized and applied in the manufacturing processes of the canned freshwater fish which uses raw materials with the same or similar characteristics, especially with relatively high fat content, and thus the market is enriched.

The invention belongs to a fish artificial incubation method field relating to the artificial incubation method of ictalurus punctatus. After the parent fishes are in natural spawning fertilization in a spawning pond, the egg masses collected are disposed in an incubating plant for hatching, which is characterized in that the hatching water salinity is 0.1 to 8%, the alkalinity and hardness are larger than 20mg / L calcium carbonate, the hydrogen sulfide is smaller than 0.005mg / L, the iron is smaller than 0.2mg / L, the ammonia is smaller than 0.5 mg / L and the dissolved oxygen is larger than 5mg / L. The water temperature should be maintained from 24 degree to 30 degree. The membrane rupture prophase of this incubation method is implemented inside a cement pool or a circuit in which the conditions such as water temperature are relatively stable. After the melanose of eyes are emerged or the egg masses are turned into red, the fries are moved to a hatching tray for the rupture of membrane. Therefore, the fry activities are easy to observe, and the fry collecting can also be facilitated.

The invention discloses a method for carrying out integrated aquaculture of ictalurus punctatus in a pond industrial aquaculture system. The method is scientific and feasible in principle, easy to operate and implement in production, and capable of being adopted and applied by farmers easily in economic benefit. The method comprises the steps of preparation before planting and aquaculture, fry stocking, purification zone floating bed rice planting, planting and aquaculture management, aquatic species capturing and rice harvesting. Accordingly, by means of the proper stocking density of ictalurus punctatus fingerlings in an aquaculture water tank, the stocking size is improved, and by means of the annual aquaculture, commercial longsnout catfish with the best-selling size being about 1.5 kg / fish are sold at a high price at different times; by properly stocking a proportion of silver carp, bighead carp, myxocyprinus asiaticus, penaeus monodon and snails and planting 5-8% of floating bedrice in an ecological purification zone simultaneously, and by means of the integrated planting and aquaculture measure, the in-situ remediation effect on aquaculture water is better exerted, the ecological benefit, comprehensive output and economical benefit of the whole breeding system are significantly increased, and the method has strong practical application and popularization value.

The invention relates to a method for intensive cultivation of ictalurus punctatus. The method comprises a step (1) of performing fingerling cultivation, a step (2) of performing adult fish cultivation, a step (a) of having pond conditions; a step (b) of performing preparation prior to stocking; a step (c) of performing stocking of fingerlings; a step (d) of feeding baits: adopting mixed domestication feeding of compound pellet feed and Chinese herbal medicine baits; a step (e) of performing daily management, and timely increasing the feeding amount of the Chinese herbal medicine baits by 15-20% of an original usage amount when fish disease is found. The Chinese herbal medicine baits are prepared from, by weight parts, 3-5 parts of nucleic acid yeast extract, 0.5-1 part of multi-vitamins, 10-15 parts of Chinese herbal medicine extract, 40-60 parts of enteric soluble polymers and 5-8 parts of vitamin E acetic ester. The Chinese herbal medicine baits used in the cultivation method are reasonable in compatibility, balanced in nutrition and low in processing cost, can remarkably improve immunity of ictalurus punctatus and greatly reduce the probability of edwardsienosis infection of ictalurus punctatus, is free of drug residue, leads to no drug resistance and is high in safety.

The invention discloses a method for preparing quick-freeing concentrated fish soup using ictalurus punctatus trunk bones. The method is as follows: slicing ictalurus punctatus, removing the fish head, fish tail and fish fins from the fish skeleton to obtain a trunk bone about 15 cm in length, washing, draining off, bagging, quick freezing and storing in a freezing manner for later use; then placing the trunk bone in a 0-4 DEG C refrigerator for unfreezing, frying with oil slightly, draining off oil, adding a certain proportion of water, cooking with big fire until boiling, stewing with medium fire, and filtering; taking and cooking the filtrate with soft fire to a certain concentration, adding salt, wei jia xian, monosodium glutamate and cassava modified starch, mixing uniformly, cooling, quick freezing, and storing in a freezing manner. The quick-freeing concentrated fish soup prepared using the method provided by the invention has the advantages of being instant, fresh, delicious, nutritive and convenient to eat, the comprehensive utilization value of the ictalurus punctatus is effectively improved, industrial production is facilitated.

The invention discloses a Danio rerio B-lymphocyte activating factor (zBAFF) cDNA (complementary deoxyribonucleic acid), and a cloning method and application thereof in recombination, and relates to the field of biological gene engineering. The cloning method of the zBAFF gene comprises the following steps: extracting total RNA (ribose nucleic acid) of the spleen of Danio rerio by a TRIzol method, and carrying out reverse transcription; and analyzing BAFF cDNAs of rainbow trout, Atlantic salmon and Tetraodon nigroviridis, and conversed regions of BAFF cDNAs of rainbow trout, Atlantic salmon and Ictalurus punctatus by Clustalw software, designing degenerate primers, and carrying out PCR (polymerase chain reaction) amplification to obtain the 807bp full-length cDNA. The Danio rerio B-lymphocyte activating factor and proliferation inducing ligand cDNA can be used for producing recombinant Danio rerio BAFF by the existing gene engineering method.

The invention relates to a directional hybridization propagation method of Ictalurus punctatuses. The method mainly comprises the following steps: step 1, the Ictalurus punctatuses are put in a copulatory device, and brightness and noise of the copulatory device are controlled; and step 2, laid eggs are put in single hatching troughs for hatching so as to obtain ideal Ictalurus punctatus fries. The invention is beneficial to improve the quality of offspring and prevent a degeneration phenomenon due to close propagation from generating.

The invention belongs to the technical field of aquaculture, and relates to a feed additive for preventing and treating bacterial gill-rot disease of ictalurus punctatus and a preparation method of the feed additive for preventing and treating the bacterial gill-rot disease of the ictalurus punctatus. The feed additive for preventing and treating the bacterial gill-rot disease of the ictalurus punctatus is prepared from the following components in parts by mass: 35 to 45 parts of ginger juice, 26 to 32 parts of lalang grass rhizome, 26 to 32 parts of cactus juice, 25 to 33 parts of acorus calamus, 25 to 32 parts of onion juice, 22 to 30 parts of portulaca oleracea, 22 to 28 parts of dark plum, 22 to 28 parts of dried orange peel, 20 to 28 parts of Chinese gallnut, 18 to 28 parts of almond powder, 16 to 26 parts of euphorbia helioscopia, 16 to 26 parts of acanthaceae sericocalyx chinensis, 10 to 18 parts of fish oil and the like. According to the feed additive and the preparation method disclosed by the invention, the occurrence of the bacterial gill-rot disease of the ictalurus punctatus can be effectively prevented; in addition, the bacterial gill-rot disease of the ictalurus punctatus can be effectively treated.

The invention provides a preparation method of a fermented traditional Chinese medicine for preventing and treating intestinal diseases of aquatic products. The fermented traditional Chinese medicineis also called a bacterial medicine, water is added into a traditional Chinese medicine composition, compound fermentation strains are inoculated, and fermentation is performed to obtain the fermentedtraditional Chinese medicine. The invention further provides the corresponding fermented traditional Chinese medicine. The fermented traditional Chinese medicine has the functions of internally strengthening digestive absorption, nourishing stomach and protecting liver and externally resisting mushroom poisons and killing pathogens, and is used for preventing and treating the intestinal diseasesin aquaculture and applicable to prevention and treatment of the intestinal diseases of special aquaculture animals such as various saltwater fishes, freshwater fishes, shrimps, crabs, sea cucumbers,turbots, bastard halibuts, large yellow croakers, snappers, sea eels, globefishes, rice field eels, soft-shelled turtles and ictalurus punctatus. The fermented traditional Chinese medicine has good effects on the intestinal diseases such as white intestine diseases, empty stomach, white discharge and hangover caused by bacteria, viruses and antibiotics, and particularly has special effects on fatal diseases of the shrimps caused by enteritis, white discharge, empty intestines and empty stomach.

The invention relates to an instant Ictalurus punctatus stomach, which is prepared from fish stomach and a pickling material in a weight ratio of 100:8. The processing steps include: (1) cleaning the inside and outside of an Ictalurus punctatus stomach, removing fish blood and other foreign materials, cutting off attached redundant mucosae, fish intestines and other tissues, performing cleaning, and draining water for standby use; (2) weighing the treated stomach according to the weight ratio of the fish stomach to the pickling material of 100:8, stirring the materials evenly, conducting pickling in a 0-4DEG C cold storage for 6h, and turning the stomach once every 2h to pickle it evenly; (3) draining excess pickling liquid from the fish stomach, placing the fish stomach in a baking oven, and conducting baking at 55-60DEG C for 2-3h; (4) pouring vegetable oil into a pan, adding 0.1g of TBHQ into per 1Kg of oil, frying the baked fish stomach in step (3) in a 150DEG C oil pan for 10-20sec for further removal of fishiness and curing; (5) using an aluminum foil bag to perform vacuum packaging on the fried fish stomach cooled to less than 60DEG C; and (6) carrying out sterilization, cooling, labeling and code marking.

The invention relates to a feed additive for preventing haemorrhagic septicaemia of ictalurus punctatus and further relates to a preparation method of the feed additive for preventing haemorrhagic septicaemia of ictalurus punctatus. The feed additive for preventing haemorrhagic septicaemia of ictalurus punctatus is composed of the following ingredients in parts by mass: 30-36 parts of couch grass root, 23-29 parts of folium artemisiae argyi, 23-29 parts of garlic juice, 21-29 parts of white gourd seeds, 21-29 parts of lophatherum gracile, 20-27 parts of fructus cnidii, 20-27 parts of divaricate saposhnikovia root, 19-27 parts of sanguisorba officinalis, 19-25 parts of lawn pennywort herb juice, 18-26 parts of rodgersia pinnata, 18-26 parts of Japanese pearlwort herb, 17-27 parts of common cephalanoplos herb, 16-25 parts of heartleaf houttuymia herb and the like. The feed additive for preventing haemorrhagic septicaemia of ictalurus punctatus can effectively avoid haemorrhagic septicaemia.

The invention discloses a method for rearing parent fishes of ictalurus punctatus. The method comprises the following steps of (1) selecting high-quality parent fishes and putting the high-quality parent fishes into a breeding pond; (2) after the parent fishes are stocked for 5-10 days, starting breeding and feeding, and using fresh fish blocks for feeding from 30 days before spawning of the parent fishes to the breeding period; (3) during the breeding period of the parent fishes, inspecting the activity conditions of the parent fishes late night every day, and when it is found that the parent fishes have chasing reactions on the water surface, putting artificial fish nests into the pond for the parent fishes to lay eggs; and (4) taking out egg masses in the artificial fish nests, putting the egg masses into a water basin filled with pond water, and feeding the egg masses into a hatching room for hatching under the condition of avoiding direct sunlight. The technology for rearing the parent fishes of the ictalurus punctatus is simple and easy to operate, has good promotion effects on optimizing the breeding process, reducing the labor amount and improving the survival and reproductive capacity of the parent fishes, and has good economic benefits and social benefits.

The invention discloses an ictalurus punctatus bait. The bait is prepared from pig lung powder, pig blood power, pig bone power, freshwater shrimp powder, freshwater snail powder, duck blood powder, animal corrosion powder, bloodworm powder, water fragrant powder, duckweed powder, fish hunger accelerator powder, fish phagostimulant powder, swelling powder, milk powder, soybean protein powder and sorghum protein powder by the following steps of: combining all the powder together in proportion; placing the mixture into a blender for blending for 90 minutes; taking the mixture out and placing the mixture into a drier, wherein the temperature of the drier is controlled to between 45 and 55 DEG C, the drying time is 1 to 2 hours and the dryness of the dried material is controlled to between 60 and 70 percent; taking the dried product out and placing the dried product into the blender again for blending for 50 minutes; taking the obtained product out and placing the obtained product into a swelling machine for swelling, wherein the temperature of the swelling machine is controlled to between 60 and 75 DEG C, the swelling time is 1 hour and the dryness of the swelled material is controlled to between 80 and 90 percent; and finally, taking the swelled product out, airing and packaging the swelled product.

The invention discloses a runway breeding method of ictalurus punetaus. According to the runway breeding method, 4-8 breeding launders are built side by side in the middle of a pond through cement pouring; two ends of each launder are blocked by fish screens; a water-driving oxygenation area is arranged outside one side of each fish screen; pond water is pushed to form high-dissolved-oxygen water flow to enter the launders by utilizing a water-driving oxygenation device; and a sewage suction water channel is arranged outside the other side of each fish screen, breeding excrement and residues are discharged through a waste and excrement collection device, and during the breeding period of channel catfish, the water flow speed of the launder is controlled through a water-driving device on juvenile fish and adult fish and different time periods. The runway breeding method of ictalurus punetaus can realize high-density and high-quality breeding of the ictalurus punetaus, can avoid the complex ex-situ purification circulation process of a breeding water body, can realize a large amount of movement of the ictalurus punetaus during breeding so as to improve the feed utilization and reduce manual management cost, and can remarkably improve the economic and environmental benefits of ictalurus punetaus breeding.

The invention relates to a comprehensive control method for ictalurus punctatus fin rot. The method comprises the steps of 1 primary culturing, 2 second culturing, 3 three stage culturing, 4 intensive breeding and 5 daily management; composite nutrition enhancer bait is prepared from, by weight, 30-40 parts of soybean meal, 50-60 parts of fish bone meal, 20-30 parts of poultry intestine powder, 10-20 parts of mango peel powder, 10-20 parts of grape peel powder, 8-12 part of houttuynia cordata powder, 8-12 parts of chitosan, 8-12 parts of propolis powder, 5-8 parts of rice bran polypeptides, 4-6 parts of mannoproteins, 1-3 parts of multivitamin BC and 1-2 parts of composite mineral salts through processing. The composite nutrition enhancer bait is good in feed attraction property, high in absorption and utilization rate and capable of significantly improving water quality of a breeding pond. By feeding the composite nutrition enhancer bait in the breeding period to strengthen nutrition and enhance ictalurus punctatus constitution and immunoprophylaxis capacity, comprehensive control over the ictalurus punctatus fin rot is achieved.

The invention provides a kit for rapidly detecting Edwardsiella ictalurus of fishes and a detection method. The kit comprises a slide treated by polylysine, a rabbit anti-Edwardsiella ictalurus polyclonal antibody, an HRP labeled goat anti-rabbit lgG, a confining liquid and a developing liquid. The slide is used as a solid-phase carrier, so that the detection cost is greatly reduced; the rabbit anti-Edwardsiella ictalurus polyclonal antibody prepared by the invention can recognize a plurality of epitopes of the same antigen, and is strong in specificity and high in sensitivity; TMB is used asa peroxidase substrate, TMB is used as a developing solution, a soluble light blue product can be generated, and the light blue product is adsorbed on the filter paper, so that the color change can bevisually observed by naked eyes. The detection method is low in equipment requirement, low in cost, simple and convenient to operate, green, environment-friendly and high in sensitivity.

The invention discloses an inactivated vaccine for ictalurus punctatus hemorrhage disease as well as a preparation method and application thereof. The preparation method comprises the following steps: A, culturing CCK (cholecystokinin) cells; B, culturing, harvesting and storing CCRV-730 (channel catfish reovirus-730) virus; C, detecting titer TCID (tissue culture infectious dose) 50 of a virus solution, wherein the virus titer is the titer of the inactivated vaccine; D, inactivating CCRV-730 virus with beta propiolactone, incubating and hydrolyzing the beta propiolactone at 37 DEG C; and E, diluting the inactivated vaccine to a certain titer with normal saline for fish to obtain the ictalurus punctatus cell cultivated inactivated vaccine which can be directly used. The inactivated vaccine is applied to prevention of the ictalurus punctatus hemorrhage disease, is good in inactivation effect, furthest avoids destruction of an inactivator to antigenic protein, and is good in immune effect. Furthermore, the toxicity of the inactivator is eliminated, and the safety of the vaccine is improved. The vaccine has the advantages of good immune effect, safety in use, low production cost, high production efficiency and the like.

The invention discloses application of fisetin in resisting of Aeromonas hydrophila infection of aquaculture animals. The fisetin can lower in-vivo and in-vitro pathogenicity of Aeromonas hydrophila through directly inhibiting activity of aerolysin and is free of influence on growth of the Aeromonas hydrophila. Further, through constructing test animal models, it is discovered that the fisetin hasa remarkable therapeutic action on Aeromonas hydrophila artificial-infected models of Ictalurus punctatus, and the death rate in 8 days is lowered to 25%. The fisetin is an effective ingredient of aplant, i.e., smoke tree, meanwhile, is also distributed in vegetables and fruits such as onions and strawberries, is a flavonoid compound with relatively high safety and can be used for treating and preventing the Aeromonas hydrophila infection of the aquaculture animals as a drug or feed additive.

The invention discloses an ictalurus punctatus fry starter feed and a preparation method thereof. The ictalurus punctatus fry starter feed comprises 30-50 parts of fish meal, 5-15 parts of milk powder, 10-30 parts of fermented soybean meal, 15-19 parts of flour, 5-15 parts of soybean protein concentrate, 1-3 parts of yeast hydrolysate and 1-3 parts of premix. The milk powder is added into the ictalurus punctatus fry starter feed to promote the intestinal development of the fries and improve the protein digestibility and immune globulin; the yeast hydrolysate promotes intestinal development ofthe ictalurus punctatus fries, and meanwhile, the digestion and absorption rate of food in the intestinal tract is increased; and preparation process parameters are optimized to obtain the special starter feed for ictalurus punctatus. According to the special starter feed for the ictalurus punctatus fries obtained by the technical scheme provided by the invention, in the same culture period, the weight reaches 100-150g, the survival rate reaches 86-93%, the growth speed is 1.02-1.26, and data support is provided for development of the starter feed for the ictalurus punctatus fries in 3-20 daysafter the ictalurus punctatus fries are put into a pond.

The invention provides a preparation method of a vaccine for inactivating Edwardsiella icataluri. The preparation method comprises the following steps: separating and purifying Edwardsiella icataluri separated from in vivo infected fish, carrying out small volume activation culture, carrying out enlarged culture by a bioreactor, carrying out inactivation, centrifugation and collection on Edwardsiella icataluri, extracting genomic DNA from pichia yeast, adding the genomic DNA of the pichia yeast to the completely inactivated Edwardsiella icataluri, and mixing to prepare the vaccine for inactivating Edwardsiella icataluri. The vaccine of the invention, which has the characteristics of usage safety, no medicine residual, and substantial immunization control effect, allows the generation of Edwardsiellosis icataluri of Ictalurus punctatus to be effectively prevented, and the immunization protecting rate can reach 80%. Immunizations carried out by adjustable continuous injectors are convenient, so the immunization efficiency and the dosage accuracy of the inoculation vaccine are substantially improved. The preparation method of the vaccine of the present invention has the advantages of simple operation and low cost; and the vaccine which can be used for controlling diseases of Ictalurus punctatus has a high business value.

The invention discloses a method for intercropping ictalurus punctatus and penaeus vannamei, and the method is characterized by comprising the steps of pond selection, breeding preparation, fry stocking, water quality regulation and control, feed feeding, disease prevention and control and the like. According to the method for intercropping the ictalurus punctatus and the penaeus vannamei, an original ecological environment of a pond can be changed, the constitution of the penaeus vannamei is enhanced, the body vitality of the penaeus vannamei is improved, the body immunity of the penaeus vannamei is enhanced, the digestion, absorption and utilization rate of bait can be increased, and disease is reduced. Residual feed, excrement, organic chippings and the like of the penaeus vannamei arefully utilized after polyculture of the ictalurus punctatus, the content of pollutants is reduced, the virtuous cycle and relative stability of pond ecology are promoted, so that the penaeus vannameiis prevented from pathogeny, and the quality and comprehensive economic benefits of vannamei products are improved. The breeding method is simple, the quality is high, the disease is less and the benefit is good.

The invention belongs to a fish artificial incubation method field relating to the artificial incubation method of ictalurus punctatus. After the parent fishes are in natural spawning fertilization in a spawning pond, the egg masses collected are disposed in an incubating plant for hatching, which is characterized in that the hatching water salinity is 0.1 to 8%, the alkalinity and hardness are larger than 20mg / L calcium carbonate, the hydrogen sulfide is smaller than 0.005mg / L, the iron is smaller than 0.2mg / L, the ammonia is smaller than 0.5 mg / L and the dissolved oxygen is larger than 5mg / L. The water temperature should be maintained from 24 degree to 30 degree. The membrane rupture prophase of this incubation method is implemented inside a cement pool or a circuit in which the conditions such as water temperature are relatively stable. After the melanose of eyes are emerged or the egg masses are turned into red, the fries are moved to a hatching tray for the rupture of membrane. Therefore, the fry activities are easy to observe, and the fry collecting can also be facilitated.