110 results about "Trout" patented technology

A real-time assay coupled with a non-invasive tissue sampling was developed for the detection and quantification of fish viruses. As a proof of principles, data were presented for the detection and quantification of infectious hypodermal necrosis virus (IHNV) in trout. The primers were designed for IHNV nucleocapsid (N), and surface glycoprotein (G) genes, and trout &bgr;-actin and elongation factor-l&agr; (EF-I &agr;) were used as internal control for the assay. The reaction conditions for the real-time RT-PCR were optimized using cDNA derived from IHNV-infected Epithelioma papulosum cyprinid (EPC) cells. Using both N- and G-gene primers, IHNV was successfully detected in liver, kidney, spleen, adipose tissue and pectoral fin samples of laboratory-challenged and wild samples. The dissociation curves with a single melting peak at expected temperature (85° C. for the N-gene and 86.5° C. for the G-gene) confirmed the specificity of the N- and G-gene amplicons. The IHNV N- and the G-gene expression levels in different tissues of laboratory challenged samples were in the order of spleen, liver, kidney, adipose tissue and pectoral fin, however in the field-collected samples the order of gene expression was liver, kidney, pectoral fin, adipose tissue, and spleen. The N- and G-gene expressions in spleen were found to be dramatically lower in the field-collected samples compared to the laboratory-challenged samples indicating a potential difference in the IHNV replication in the laboratory as opposed to field conditions. The real-time PCR assay was found to be rapid, highly sensitive, and reproducible. Based upon the ability to detect the virus in pectoral fins a non-invasive detection method for IHNV and other fish viruses is developed. Such a non-invasive tissue sampling coupled with real-time PCR assay is very valuable for large-scale virus screening of fish in aquaculture facilities as well as for epidemiological studies.

The invention provides a production method for purified recombinant proteins of commercial value prepared in enclosed aquatic systems. The production system provides biosecure containment of genetically engineered aquatic organisms for an alternative production method for complex proteins requiring post-translational modification that is best done in eukaryotic expression systems. Possible aquatic organisms that can be utilized by this invention are crustaceans (e.g. penaeid shrimp or Artemia) and teleost fish (e.g. trout or tilapia).

The invention relates to a long-distance transport method for fresh cold water fishes. The long-distance transport method is characterized by comprising the following steps of putting the cold water fishes in a fish bag filled with water which is 1 / 5-1 / 4 of the weight of the fishes; fully inflating the fish bag with oxygen, and then, sealing; keeping the fish bag water-tight and air-tight; putting the fish bag in a transport case, and putting 1-2 ice bags in the transport case so as to enable the water temperature in the fish bag to be kept to be 0-18 DEG C during a whole transport process. The fish bag is made of a water-tight and air-tight soft material; the water used in the fish bag is clean spring water or clean river water of which the water temperature is 0-18 DEG C. The long-distance transport method for the fresh cold water fishes provided by the invention is suitable for keep-alive transport of the cold water fishes, such as Chinese sturgeon, Polyodonspathala, salmon, rainbow trout, golden trout, giant salamander (megalobatrachus) and the like, and also suitable for the keep-alive transport of other non-cold water fishes.

A method for inducing the tetraploid salmoniform rainbow trout by hot shock or static water pressure process features that the fertilized ova of rainbow trout is treated by hot shock at 30 deg.C for 5-6 min or static water pressure under 600-650 kg / sq.cm for 5-6 min.

A special feedstuff used for hucho taimen larvae relates to a fish feedstuff. The invention solves the problems of low growth speed and high death rate of hucho taimen by feeding the hucho taimen withrainbow trout feedstuff. The hucho taimen special feedstuff comprises the following components in weight percentage: 2-5 percent beer yeast, 2-6 percent wheat-middling, 2-8 percent spray drying bloodflour, 60-70 percent fish flour, 10-20 percent fish oil, 1-4 percent soybean lecithin, 0.2-0.8 percent sodium alginate, 0.01-0.05 percent L-carnitine, 0.05-0.2 percent KCL, 0.02-0.1 percent inositol,0.02-0.07 percent enzyme preparation, 0.01-0.04 percent high stability VC, 0.1-0.5 percent magnesite, 0.01-0.05 percent antioxidant, 0.05-0.02 percent Kemei, 0.8-1.5 percent zeolite. Compared the special feedstuff with the imported rainbow trout feedstuff, by feeding the hucho taimen with the special feedstuff, the survival rate of the hucho taimen can reach above 95% after 4 weeks, and the survivalrate of the hucho taimen can reach above 92% after 8 weeks; the average weight is increased by 18.35%, and the weight gain rate is improved by 9.49%.

The invention relates to the field of aquaculture, in particular to a manual induced spawning method for platichthys stellatus parental fishes. Trout gonadotrophic hormone releasing hormone analogues (S-GnRHa) and domperidone (DOM) are used for performing induced spawning on the platichthys stellatus parental fishes, and female fishes older than 2.5 years and male fishes older than 2 years are selected for being put into a parental fish breeding pond for ripening acceleration. The sexual maturity is controlled in an intensifying mode through water temperature, illumination stimulation and baits. The S-GnRHa and DOM are used for performing induced spawning on the platichthys stellats parental fishes, medication injection is performed on the female and male parental fishes two times according to weights, so that the effect of performing manual induced spawning on the platichthys stellatus parental fishes is achieved and artificial insemination is performed. According to the method, the platichthys stellatus parental fishes are matured synchronously, spawning can be performed within a preset period in a concentrated mode, the spawning quantity is large, the spawning period is shortened by 10-15 days, the insemination rate reaches 80%, the hatching rate reaches 70%, manual induced spawning is performed on the parental fishes, the success rate of performing induced spawning on the male fishes reaches 98%, and the success rate of performing induced spawning on the female fishes reaches 95%.

The invention relates to the technical field of for cultivating salmon and trout and provides a solid mixed cultivation method for salmon and trout flatfish. The salmon and trout and the flounder are cultivated in a same cultivation pool together. The use ratio of the cultivation water can be greatly improved by the complementary feature of life water of salmon and trout and the flounder, and then the unit water yield and effect are improved; and the maximum economic benefit is realized by using the limited water resource.

The invention provides a fusion polypeptide transgene sequence for calcitonin-gene-related peptide and salmon calcitonin and a transgene engineering strain thereof, and relates to a fusion polypeptide transgene sequence and a transgene engineering strain. The invention solves the problem that: the prior calcitonin-gene-related peptide and salmon calcitonin fusion polypeptide are not suitable for industrialized production with microbes and animals as reactors. The calcitonin-gene-related peptide and salmon calcitonin fusion polypeptide transgene sequence is shown as SEQ ID NO.1. The calcitonin-gene-related peptide and salmon calcitonin fusion polypeptide transgene engineering strain is an agrobacterium containing fusion gene plasmids, wherein the fusion genes in the plasmids are shown as SEQ ID NO.1. The calcitonin-gene-related peptide and salmon calcitonin fusion polypeptide transgene engineering strain can be used for mediating a plant with the cost of the engineering strain being 1 / 10 to 1 / 50 of that of bacillus coli.

The invention relates to drug manufacturing field, especially a trout calcitonin powder fog agent and the manufacturing method. It is made up from 3 portions of trout calcitonin, 500-2000 portions of mannitol, and 500-2000 portions of amino acid. It has good solubleness, bulk density, sprayabiligy, and is not easy to absorb moisture.

The invention provides a cultivation method for hybridized all-female golden trout fries. An androgen is utilized to induce golden trout parent fish in a mature period so as to obtain golden trout pseudo-milter, steelhead parent fish eggs are fertilized with golden trout pseudo-milter sperms, hatching is performed, and the hybridized all-female golden trout fries are obtained. The eyed rate of the fertilized eggs during a hatching period is over 81%, the fry hatching rate is over 92%, the female rate of the obtained fries is 100% by detection, the rate of golden trout (with golden yellow body color) of the fries is over 90%, the weight gaining rate is over 122% after the fries are cultivated for 30 days, and the survival rate is over 98%. Compared with fries generated by purebred golden trout mating, the cultivation method for the hybridized all-female golden trout fries maintains the golden trout rate to be unchanged, enables the golden trout fries having double advantages of being hybridized and all-female to be obtained, and has good economic benefit and market value.

A forage for Atlantic salmon contains proportionally fish powder, blood powder, soybean, fish fat, wheat flour, and the additive containing vitamin composition and choline chloride.

The invention provides calcitonin gene-related peptide and salmon calcitonin fusion polypeptide, relating to fusion polypeptide. In order to generate a product containing both calcitonin and the calcitonin gene-related peptide, the invention provides the calcitonin gene-related peptide and the salmon calcitonin fusion polypeptide. The amino acid sequence of the calcitonin gene-related peptide and the salmon calcitonin fusion polypeptide is shown in SEQ ID No:1; moreover, C-terminal amidation is carried out to the calcitonin gene-related peptide and the salmon calcitonin fusion polypeptide. Experiments verify that the calcitonin gene-related peptide and the salmon calcitonin fusion polypeptide preserve respective activities of the calcitonin gene-related peptide and salmon calcitonin.

The invention discloses a formula and a production method for a puffed feed for speckled trouts and aims to provide the puffed feed which is suitable for the digestion physiological characteristics of the speckled trouts, good in palatability, high in disease resistance and high in nutrient digestibility, and can lower the use amount of fish meal, solve the problem of the shortage of fish meal resources, reduce the addition of inorganic phosphorus sources, lower phosphorus pollution to an aquaculture water body so as to promote the development of the speckled trout cultivation industry in China. According to the invention, due to the optimization of the conventional formula of the puffed feed, the problem of overhigh content of fish meal in the conventional puffed feed is solved, and the nutrient digestibility and palatability of the puffed feed are improved. The puffed feed product disclosed by the invention has the advantages of remarkably prompting the quick growth of the speckled trouts, improving the cultivation survival rate and cultivation digestibility, lowering cultivation cost and discharging of nitrogen and phosphorus, improving the cultivation environment, and the like.

The invention provides a cultivating method for offspring seeds of spotted trout. By the cultivating method, a large quantity of high-quality and healthy spotted trout fries can be acquired, and survival rate of juvenile fishes is high, so that cultivation popularization and factorization of the spotted trout can be realized. The cultivating method includes the following steps: (1), transporting eyed eggs of the spotted trout, (2), incubating the eyed eggs, (3), cultivating the fries at an early stage, (4), cultivating the fries at a late stage, (5) cultivating advanced fries, and (6), cultivating the juvenile fishes. By the cultivating method, incubation and cultivation conditions for the eyed eggs of the spotted trout are acquired; experiments show that incubation rate of the eyed eggs of the spotted trout can be increased to 97%, and the survival rate of the fries can reach 92%; and through the cultivating method, the fries grow quickly, cultivation cycle can be shortened, and cultivation cost can be reduced.

The invention discloses a method for breeding large-size trout in an offshore manner in a sea area in the temperate zone. According to the method, when the temperature of sea water on the surface layer is higher than 18 DEG C, the bred trout is moved into offshore net cages or other breeding devices for further breeding, wherein by means of the offshore net cages or other breeding devices, low-temperature sea water resources on the lower layer can be utilized. The method mainly comprises the steps that in autumn, when the temperature of sea water on the upper layer in a breeding area of the sea area in the temperate zone drops to 18 DEG C or below, trout fingerlings bred in fresh water are acclimatized into the sea water and moved into the net cages or other breeding devices to be further bred to early summer of the next year; in early summer, before the temperature of the water of the upper layer in the breeding sea area is higher than 18 DEG C, the bred trout is moved into the offshore net cages or other breeding devices to be further bred to the next year, wherein by means of the offshore net cages or other breeding devices, the low-temperature sea water resources on the lower layer can be utilized; and around June, before the temperature of the sea water on the upper layer rises to 18 DEG C, the large-size commercial trout is harvested, and the trout can also be continuously bred to the required size. The method can be used for producing the large-size commercial trout, has the beneficial effects of being high in economic benefit, environmentally friendly, low in cost and the like and is an offshore high-quality fish breeding technology which is desiderated in China.

The invention discloses a dendrobium huoshanense essence mask. The dendrobium huoshanense essence mask is prepared by the following raw materials including, by weight, 15-25 parts of dendrobium huoshanense extract, 3-6 parts of centella asiatica baking powder, 10-15 parts of placenta extract, 3-7 parts of trout ovary peptide, 5-10 parts of parsley seed extract, 2-6 parts of wall-broken spirulina extract, 0.05-0.1 part of cucumber extract liquor, 1-5-2.5 parts of trehalose, 5-10 parts of ginseng, 5-10 parts of astragalus and 5-10 parts of salicylic acid betaine. Eye cream obtained through the dendrobium huoshanense essence mask has the functions of remarkably promoting cell neogenesis and repairing and reconstructing tissue, so that the eye cream has good moisturizing, anti-aging and repairing functions. Through synergistic effects of the centella asiatica baking powder, the placenta extract, the trout ovary peptide and the homemade dendrobium huoshanense extract, cucumber extract liquor, parsley seed extract and wall-broken spirulina extract, the obtained mask has the functions of remarkably promoting cell neogenesis, repairing and reconstructing the tissue, and moisturizing.

The flexible fish shaped container for disposal of fisher's litter, has an internal hollow body chamber surrounded by the external shape of a trout and is constructed of flexible rubber material which incorporates design functions into a trout fish shape for pleasing user aesthetics, attachment location, ease of one handed operation and user safety.The fin locations of the trout fish shaped container guide finger placement and the natural grip of the hand with thumb and forefinger resting at the tips of the trout shaped tail for one handed operation.Access occurs through a normally closed slit in the tail. When the tips of the fish tail are squeezed toward each other the slit opens thereby providing access to the internal cavity for disposal of fisher's litter and waste items. When thumb and finger pressure is released the slit closes.

The invention discloses a hucho trout biological slice making technology, and relates to a slice making technology. The technology of the invention solves the problems existing in the conventional slice making technology. The problems are that the slice is incomplete, the slice is not clear after slice sealing, and the agent is harmful. The making method includes the steps of: (1) fixing a hucho trout sample by employing the Bouin's solution, washing the sample by running water, and placing the sample into a first liquid, a second liquid, and terpineol in turn; (2) taking out the sample from the terpineol and placing into a mixed liquid which has equal volumes of terpineol and paraffin, and then placing the sample into the paraffin for embedding, slicing the sample after cooling; (3) carrying out the HE dyeing to the slice, and obtaining a finished product after sealing the slice by Euparal. The hucho trout glass sample obtained through the hucho trout biological slice making technology has the characteristics of fresh dyeing color, clear acid and alkaline cells, complete slice composition, clear image, and well-defined outline. The making technology of the invention saves many steps compared with the conventional making method, and reduces environmental pollution.

The invention discloses a composition for preventing and treating trout saprolegniasis. The composition comprises the following ingredients in parts by mass: 1 to 5 parts of diallyl-2,3-thioether and 0.2 to 1 part of salicylic acid. A preparing method comprises the following steps of: proportionally mixing the diallyl-2,3-thioether and the salicylic acid; stirring the mixture to be made into raw medicine; putting 0.15 to 0.25g of raw medicine in per cubic meter of area; adding water for diluting the solution to 1000 times to form medicine liquid; spreading the prepared medicine liquid into a fishpond; meanwhile, breaking the water supply, and turning on aeration equipment; injecting fresh water after 15 to 45min; adding the medicine once every other day; and performing continuous operation for 3 to 5 times. The diallyl-2,3-thioether and the salicylic acid are scientifically matched, so that the permeability of the composition is high; the sterilization effect is exponentially increased; the residue content of the diallyl-2,3-thioether and the salicylic acid in the environment and trout bodies is low; the medicine cost is low; and the composition can be used for replacing malachite green. Compared with traditional Chinese herbal medicine and Meibinglike in an aspect of the curative effect on the saprolegniasis, the composition has the advantages that the cure rate and the survival rate are high; and the national aquatic product fish medicine new standard is met.

The invention relates to a trout production technology, belongs to the field of fish breeding, and particularly relates to a trout roe production method. The method is characterized by comprising: after bred trout achieves sexual maturity, selecting trout parent fishes at a breeding season, wherein ovary of the selected trout parent fish develops to a V stage; adopting an extrusion method to extrude trout roes; carrying out immersion washing on the trout roes at a temperature of below 4 DEG C with an iodine-free salt solution with a concentration of 0.5-5%; carrying out pickling with an iodine-free salt solution with a proper concentration; draining water with gauze; and carrying out ultraviolet disinfection and packaging. The production method has the following beneficial effects that: the produced trout roe achieves a pollution-free food standard, and has characteristics of natural shape and high nutrition value; the operation method is simple and convenient, and complex equipment is not required; and the trout roe is produced so as to extend a trout industry chain, improve breeding benefits, avoid disadvantages of trout roe production through wild trout fishing, protect trout resources and maintain ecological balance.

The invention discloses a health food with sorbering up and liver protection functions, and a preparation method thereof. The health food with sorbering up and liver protection functions comprises the following components in parts by weight: 25-35 parts of trout, 14-20 parts of longan pulp, 10-15 parts of black bamboo roots, 10-15 parts of costus roots, 10-15 parts of herba rabdosiae, 5-10 parts of purslane, 5-10 parts of durian powder, 5-8 parts of rhizoma bolbostemmae, 2-4 parts of erythritol, 2-4 parts of pagodatree flower bud extract, 2-4 parts of Agrimonia pilosa Ledeb extract, and 2-4 parts of corn stigma extract. Compared with the prior art, the prepared health-care food has no toxic and side effects, not only has nutritional value, but also has sorbering-up and liver protection functions, tests prove obvious effects of sorbering-up and liver protection functions.

The invention takes a method for developing and breeding rainbow trout and transforming the rainbow trout into trout as a task. Through screening a rainbow trout body with the weight of 200g-300g among the rainbow trout, the screened rainbow trout body is raised for 3-10 days in fresh water within the temperature range of the seawater temperature + / -3 DEG C and is stopped to be fed with fodders during the period, then the screened rainbow trout body is transferred to be raised in seawater, and thus the task provided by the invention can be solved.

The invention discloses flammulina velutipes tea powder and a preparation method thereof. The flammulina velutipes tea powder is prepared from the following raw materials in parts by weight: 80-100 parts of flammulina velutipes, 10-15 parts of medlar mud, 20-30 parts of yacon, 15-25 parts of boilen ta, 8-12 parts of trout meal, 20-40 parts of blueberry juice, 30-60 parts of pomegranate wine, 4-6 parts of spirulina powder, 20-40 parts of milk, 2-3 parts of nitraria roborowskii, 2-3 parts of official magnolia flowers, 1-2 parts of pear flowers, 2-3 parts of pineapple peels, 1-2 parts of gynostemma pentaphylla, 8-15 parts of green tea powder, 10-15 parts of nutritional health powder and 4-6 parts of coix seed oil. The flammulina velutipes tea powder has fresh tea fragrance, tastes fragrant, sour and sweet and has fine and smooth texture and a unique flavor; the nutrition is rich and balanced; various food materials and healthy traditional Chinese medicines work synergistically, so that the flammulina velutipes tea powder has functions of strengthening the stomach, promoting digestion and enhancing the oxidization resistance of the organism and also has an effective healthcare function of reducing blood fat and an effective health function.

The invention discloses a salmon trout cultivating and feeding method, and belongs to the technical field of aquaculture. The technical point of the invention is that the salmon trout cultivation is performed in two sets of sealed recirculating aquaculture systems, and every set comprises 24 culture ponds, and water for cultivation is well water; the cultivation index is that the temperature of water for cultivation is 15-17 DEG C, the dissolving oxygen is 7-8 / mg / L, and water quality indexes of ammonia nitrogen, nitrate, and nitrite are the same condition; the food feeding parameters: the daily feeding amount includes four groups which are 0.7%, 1%, 1.5% and 1.8% of fish weight; the daily feeding frequency is 4 times, and the feeding time is set at 6: 00, 12: 00, 18: 00 and 24: 00; the overall growth condition of the fish is tested once monthly; the survival rate and fatness of fish are calculated; water quality is monitored at fixed time and regular period; the detection position is awater returning pond. The invention is used for salmon trout cultivation.

Feed for adult trout is made from, by weight, 3-12 parts of pork powder, 18-26 parts of corn flour, 15-20 parts of rapeseed cake, 11-24 parts of longan shell powder, 22-33 parts of purple cabbage, 0.01-0.02 part of vitamin A, 0.5-1.5 parts of magnesium sulfate, 0.5-1.5 parts of Rhizoma Drynariae, 9.5-12 parts of Exocarpium Benincasae, 5.5-7.5 parts of diatom powder, and 5-10 parts of a modifying additive. The feed is suitable for adult trout, can promote further growth of trout, greatly enhance the activity and reproductivity of trout, promote immune cell differentiation in trout and enhance its disease resistance, never causes the rise of nitrogen, phosphorus and potassium content in water and has low impact on water quality.

The invention provides a cage culture method of the speckled trout. The method includes production and application of culture cages, satisfying the requirements of a culture zone environment, setting culture density, feed preparation, feeding, and daily management. The speckled trough is fed by means of periodic starvation; for example, feeding is stopped for one day after the speckled trough is fed continuously for 7 days; accordingly, feed conversion rate and speckled trough yield are increased. Astaxanthin is added to the feed, so that meat color of the speckled trout is improved evidently.

The invention discloses a reagent for multiple detection of salmonidae virus and an application thereof. The reagent provided by the invention comprises a specific primer group, wherein the specific primer group comprises at least one pair from a first primer pair to a sixth primer pair; the first primer pair to the sixth primer pair are sequentially primer pairs of: nucleic acid of specifically-amplified spring viraemia of carp virus, nucleic acid of infectious hematopoietic necrosis virus, nucleic acid of viral haemorrhagic septicaemia virus, nucleic acid of infectious salmon anaemia virus,nucleic acid of salmonid alphavirus, and nucleic acid of infectious pancreatic necrosis virus. The upstream and downstream primers of the first primer pair to the sixth primer pair are sequentially the sequences as shown in Seq ID No.1 to Seq ID No.12. The reagent can carry out multiple detection on six salmonidae viruses, is simple and convenient to use, shortens the detection time of the six salmonidae viruses, improves the detection efficiency and quality, and has great significance for rapid detection and prevention and control of the six salmonidae viruses.

The invention discloses speckled trout puffing mixed feed for resource utilization of salicornia europaea. A shortcoming that existing feed fish meal is high in usage amount is overcome, and waste is turned into wealth to utilize salicornia europaea resources at a high value. The feed product is mainly made of salicornia europaea ferment powder, steam fish meal, peeled bean pulp, wheat middling, rapeseed meal, beer yeast, fish oil, animal fat, sodium alginate, mono calcium phosphate, multi-vitamins, composite minerals, allicin, bacillus licheniformis, lactobacillus, phytase and non-starch polysaccharide enzyme and the like. The salicornia europaea ferment powder is made of salicornia europaea, paederia scandens, rhodiola rosea, liquorice, epimedium herb, malt and medicated leaven and the like through a secondary fermentation process. The product has the advantages of remarkably promoting quick growth of speckled trout, improving cultivation survival rate, reducing cultivation cost, improving cultivation digestibility, reducing nitrogen and phosphorus discharge and improving a breeding environment and the like, and the speckled trout puffing mixed feed has high cost performance and popularization and application values.

The invention discloses a method for controlling the synchronous maturity of brook trout and rainbow trout. The method comprises the following steps that female rainbow trout and male brook trout are cultured in a separated mode; when the ovaries of the female rainbow trout are developed to the end of the III period and the beginning of the IV period, stimulation by running water is conducted for 2 hours one time every 3 days, and the water temperature in a culture pond is decreased to 6 DEG C; meanwhile, the average sunlight irradiation time of the male brook trout is adjusted to 5.8 hours, the male brook trout is subjected to sunlight irradiation one time every day, and stimulation by the running water is conducted for 6 hours one time every day; the female rainbow trout is fed with fresh fish one time every week; the male brook trout is fed with the fresh fish one time every day, the female rainbow trout is fed with commercial feed two times every day, and the male brook trout is fed with granulated feed two times every day. By means of the method, the synchronous maturity rate of the brook trout and the rainbow trout can be increased to 85.7%, it is ensured that the amount of the rainbow trout is enough for hybridization, and the method is beneficial to popularizing the hybridization breeding work of the brook trout and the rainbow trout.