The invention discloses a breeding method for a tropical high-oil maize self-bred line, and belongs to the technical field of crop breeding. In the method, temperate high-oil maize hybrids is used as a donor parent, a tropical maize self-bred line is used as a recurrent parent, one-generation cross breeding, two-generation backcross and five-generation selfing are performed, in backcross and selfing, the tropical maize self-bred line is used as a male parent in backcross and selfing, large-germ seeds are selected in each generation in backcrossing and selfing, namely seeds of which seed germ planes account for more than 3 / 4 of seed planes are selected, and the tropical high-oil maize self-bred line is bred. The tropical high-oil maize self-bred line bred by the method integrates the high oil content of temperate high-oil maize germplasm and the high resistance of tropical maize germplasm, is high in expression quality, resistance and yield, and particularly is obviously superior to the original temperate high-oil maize in aspects of stress resistance and yield, so the basis of the temperate high-oil maize germplasm is broadened, and the excellent basis is established for the breeding of high-oil maize hybrids in tropical and subtropical zones.