The invention discloses a Chinese medicinal preparation for treating an acquired immune deficiency syndrome (AIDS) and a preparation method thereof. Active ingredients of the Chinese medicinal preparation are prepared from the following Chinese medicaments in part by weight: 2 parts of java brucea fruit, 3 parts of India madder root, 3 parts of India canna, 2 parts of striga, 3 parts of fiveleaf gynostemma herb, 2 parts of Chinese starjasmine stem, 3 parts of copperleaf herb, 2 parts of spike, 3 parts of raphanus sativus, 2 parts of Asiatic pennywort herb, 3 parts of bupleurum, 3 parts of muskrat, 2 parts of golden thread, 3 parts of scutellaria, 3 parts of loquat leaf, 3 parts of vine root, 2 parts of dahurian patrinia herb, 3 parts of black bamboo root, 1 part of prepared rhubarb, 1 part of borneol, 1 part of iron ore, 1 part of fish bile (bile of whale), 3 parts of unibract fritillary bulb and 3 parts of capparis spinosa. The Chinese medicinal preparation is a pure Chinese medicinal preparation and has the advantages of inhibiting reproduction of viruses in vivo, enhancing immunity, preventing and treating opportunistic infections and curing the AIDS, along with little toxic or side effect, quick response and good curative effect.