222 results about "Dendrobium huoshanense" patented technology

The invention discloses a method for planting Dendrobium huoshanense on a tree. The method comprises the following steps: standard land selection and three selection, tree trunk treatment, Dendrobium fixation, water and fertilizer management and the like. The method of planting Dendrobium huoshanense on the tree provided by the invention not only overcomes the defects that Dendrobium huoshanense wild cultivation is low in yield, is controlled by natural factors, relies on weather, is difficult to pest control, low in quality of greenhouse cultivation and the like, but also maintains the quality and appearance of wild Dendrobium huoshanense, so that the yield can be guaranteed, the pollution is produced, and products have the advantages of safety and high efficiency.

The invention discloses a method for cultivating tissues to obtain seedlings of dendrobium huoshanense at one step. The method includes steps of preparing solid cultivation media; preparing liquid nutrient liquid according to a formula of the solid cultivation media; selecting and disinfecting dendrobium huoshanense capsules; preparing dendrobium huoshanense seed suspension liquid; sowing the dendrobium huoshanense seed suspension liquid in an aseptic condition; cultivating the seedlings; germinating the seedlings. The solid cultivation media are solid nutrient media, and the solid nutrient media in a formula comprise macroelements, trace elements, iron salt, organic components, hormone and additives. The liquid nutrient liquid is free of hormone, agar, chlormequat and banana and apple extracted liquid. The method for cultivating the dendrobium huoshanense has the advantages that the dendrobium huoshanense cultivation period can be shortened, the seedlings can be germinated in 6 months, in other words, the seedlings can be germinated in exactly seedling planting periods in April of a second year if seeds in October of a first year are sown, accordingly, the production cost for cultivating the seedlings of dendrobium huoshanense from the tissues can be reduced by 50%, the planting input can be reduced, and the cultivation survival rate and the cultivation germination rate can be increased.

The invention discloses a bacillus methylothophicus strain for preventing and controlling pear ring rot and soft rot and application of the bacillus methylothophicus strain. The endophytic bacillus methylothophicus strain is separated from dendrobium huoshanense roots which are subjected to surface sterilization, and the strain has the broad-spectrum resistance on multiple plant pathogens and has the good prevention and control effect on pear fruit rot caused by the ring rot and the soft rot. The endophytic bacterium serving as a biocontrol bacterium has the advantages of the broad-spectrum bacterial resistance, the efficient safety and the like and is safe to pear fruits, low in large-scale production raw material, convenient to operate, stable in prevention and control effect and capable of effectively solving the problems of residual pesticides and environmental pollution which are brought by pesticide prevention and control over pear fruit diseases. Accordingly, the huge application potential on biological disease prevention and control after pear harvesting is achieved, and a good basic bacterial strain is provided for researching and developing microbial pesticides.

The invention relates to a method for propagating dendrobidium huoshanness seedlings, which comprises the following steps: preparing explants, performing sterile seed sowing, inducing protocorms, performing artificial pollination, culturing strong seedlings, transplanting test tube seedlings and the like. In the method, strong flowering dendrobidium huoshanness plants are used as mother plants and pollinated artificially to grow fruits, seeds are collected from mature fruits and cultured in a seed germination culture medium to grow protocorms and consequently seedlings, the seedlings are cultured in a flowering culture medium in test tubes, the flowering seedlings are pollinated artificially under a sterile condition, seeds are collected from mature fruits and sowed in a sterile manner, the seeds are grown into small plants, namely seedlings that can be transplanted from the test tubes, and transplanting the seedlings from the test tubes. When the method is used, the operation is simple, the cost is low, the maturing period of the fruit of dendrobidium huoshanness is shortened, the efficiency is high, diseases and insects are prevented, dendrobidium huoshanness seeds can be produced all the year round, the transplantation survival rate of the grown seedlings may reach over 96 percent, and a new approach is provided for propagating dendrobidium huoshanness seedlings.

The invention discloses a greenhouse planting method of dendrobium huoshanense and belongs to the technical field of plant cultivation. The method includes the steps of greenhouse building, seedling growing bed frame erecting, preparation of culture media, preparation and transplantation of dendrobium huoshanense seedlings, management after transplantation, harvesting and the like. A seedling growing bed frame with the bottom hollow is erected in a greenhouse, and ventilation and breathability are facilitated. The growing vigor of the dendrobium planted through the seedling growing bed frame is much better than that of dendrobium planted through a common nursery seedling plate, and stems and leaves are plump; materials suitable for water retention and fertilizer retention are screened as the media, the growth period of the dendrobium huoshanense is shortened, and the survival rate reaches as high as 99% or above; in the whole planting process, nearly no pesticide is applied, seed soaking and sterilization are performed through traditional Chinese medicine liquid, and eating safety of the dendrobium huoshanense is improved; in the fertilizing process, only self-made vegetable cake liquid fertilizer and silkworm dung are adopted, and no chemical fertilizer is used; the content of nutrient ingredients of the dendrobium planted through the method is high, quality is good, the method is environmentally friendly, and the dendrobium huoshanense is safe to eat.

The invention discloses a selenized dendrobium huoshanense culture and a method for producing the selenized dendrobium huoshanense culture. The method comprises the steps of: inducing to obtain an aseptic seedling of dendrobium huoshanense, breeding to obtain callus of selenium-resistant dendrobium huoshanense by collaboration of dark and low-salt medium, domesticating the selenized dendrobium huoshanense callus by the low-salt medium assisted by blue light, and producing the selenized dendrobium huoshanense culture by illumination of a fluorescent lamp. The mass fraction of organic selenium in dried product of the selenized dendrobium huoshanense culture produced by the method described by the invention is not less than 50mug / g. According to the method provided by the invention, high-quality dendrobium huoshanense substitute without hormone and pesticide residue can be produced at low cost and large scale via standardization; the selenized dendrobium huoshanense culture product has activity quality of the dendrobium huoshanense and the organic selenium, and can be applied to medicines and health-care products.

The invention discloses a method for cultivating triploid dendrobium huoshanense by a crossbreeding method of tetraploid plants and diploid plants. The method comprises the following steps: (1) dendrobium huoshanense species which are from different producing areas and have characteristics of good stress resistance, rapid growth, thick stalk, long stem, many tillers, low fiber content and high active ingredient content are preferably selected as polyploidy cultivated original plant parents; (2) seeds of the different species of original plants undergo aseptic sowing in a solid medium so as to obtain diploid protocorms; (3) protoplasts of the different species of diploid protocorms are prepared and cell fusion is carried out; (4) detected tetraploid plants undergo asexual tissue culture so as to obtain tetraploid plant seedlings; (5) the tetraploid plant seedlings undergo hardening-seedling acclimatization and cultivation to flowering; (6) the tetraploid plants and diploid plants undergo artificial pollination and crossbreeding, and fructification is completed to obtain triploid dendrobium huoshanense seeds; and (7) the triploid seeds undergo aseptic sowing and culture so as to obtain a lot of triploid seedlings.

The invention relates to a method for growing cell embryos of Dendrobium huoshanense, which comprises the following operating steps: (1) inducing sterile in-vitro cuttings; (2) inducing embryonic calli; (3) inducing somatic embryos; and (4) performing synchronous propagation of somatic embryos to obtain a large amount of cell embryos of Dendrobium huoshanense. In the method, morphological lower ends of the jointed stem segments of the sterile in-vitro cuttings are used as explants, the oxidation-reduction potential of the induction culture medium is controlled, exogenous hormone is not required to be added to induce the embryonic calli of Dendrobium huoshanensem, and the exogenous hormone is prevented from being enriched into complete plants through somatic cells and endangering health of human body. By controlling ambient humidity, embryonic calla are grown into somatic embryos, the consistency of spherical cell embryos is over 90 percent, and the survival rate of regenerated plants is over 85 percent. In the invention, the process is simple and not limited by time and seasons, and industrial automatic production of g cell embryos of Dendrobium huoshanense and in-vitro cuttings of Dendrobium huoshanense can be carried out in door for satisfying needs for production and planting.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker for identifying dendrobium huoshanense C.Z. Tang et S.J.Cheng and a molecular detection method for the SNP marker, and provides application of SNP of the following SNP site in a dendrobium genome or a substance for detecting the SNP of the following SNP site in the dendrobium genome to identification or auxiliary identification of dendrobium. The SNP site in the dendrobium genome corresponds to the 113th site of a nucleotide sequence shown in sequence 3 in a sequence table; a nucleotide at the SNP site is T or G. According to the SNP marker and the molecular detection method, sequencing analysis is performed on 27 kinds of dendrobium samples comprising all species of dendrobium huoshanense C.Z. Tang et S.J.Cheng complexes to find the functional SNP molecular marker only existing in the dendrobium huoshanense C.Z. Tang et S.J.Cheng by virtue of an RAD (Restriction Site Associated) technology, and the detection method for the SNP marker is established to accurately identify the dendrobium huoshanense C.Z. Tang et S.J.Cheng and other dendrobium (comprising hybrids of the dendrobium huoshanense C.Z. Tang et S.J.Cheng and the other dendrobium).

The invention discloses a yellow-tea preparation process. The yellow-tea preparation process includes the steps of picking of fresh leaves; withering of the fresh leaves; water removing; light rolling; heaping for yellowing; primary baking, cooling and secondary baking; fragrance raising and re-drying; quality testing, weighing and packaging. Huoshan yellow shoots are taken as raw materials, so that the yellow tea prepared by the unique process has the advantages of being attractive in appearance and high in quality, yellow-bright and clear in liquor color, fragrant and mellow in taste, durable in brew and sweet in aftertaste and moist in throat; Huoshan local herbal medicine like dendrobium huoshanense, Huoshan ganoderma lucidum, bulb mite, Longhan pinellia ternate, tuceahoe, eucommia ulmoides, platycodon grandiflorum and clinopodium polycephalum are added, so that efficacy of dehumidification and detoxification, lung moistening and cough relieving, heat clearing and nerve soothing, dampness excreting and diuresis inducing, spleen benefiting and stomach harmonizing and nerve calming is achieved, and auxiliary efficacy for cough haemoptysis, chronic bronchitis, tiredness and nerve exhaustion, neurasthenia, restless sleep and the like is realized; meanwhile, moderating effects on immune system, endocrine system, central nervous system, circulatory system and urinary system to different degrees are achieved.

The invention discloses a production technology for dendrobium huoshanense herbal tea. The production method comprises seven steps of (1) selecting raw materials, (2) protecting the color, (3) fixing, (4) stir-frying to shape, (5) drying, (6) sorting, and (7) weighing, packaging and storing. Due to the step of protecting the color, the color and luster of the flowers are protected to the greatest extent; due to the microwave drying method, the labor intensity can be relieved, the labor cost can be saved, and the production efficiency can be improved; the processed dendrobe flower has no bitterness, the dendrobium huoshanense herbal tea maintains the medical value of the dendrobium huoshanense, has sweet taste, is fragrant and soft and smooth, and has good effects, the dendrobium huoshanense flower is reasonably and effectively developed, and the market blank is filled.

The invention provides an extraction method of dendrobium huoshanense polysaccharide, and in particular relates to a method for extracting dendrobium huoshanense polysaccharide by an ultrasonic wave assistance hot reflux extraction method. According to the extraction method, the extracted polysaccharide is subjected to content detection by a phenol-sulfuric acid method. After the primary impurity removal through diethyl ether addition into dendrobium huoshanense coarse powder, a proper amount of methyl alcohol is added; after ultrasonic wave assistance hot reflux extraction, filtering is performed when the liquid is still hot; washing is performed; filtering residues are put into a flask; a proper amount of water is added; then, ultrasonic wave assistance hot reflux extraction is performed again; filtering is performed when the liquid is still hot; the operations are repeated for four times; obtained filter liquid and washing liquid are merged; through rotary evaporation, a target product, namely the dendrobium huoshanense polysaccharide is obtained. The extraction method of the dendrobium huoshanense polysaccharide provided by the invention has the advantages that the content of the obtained polysaccharide is high; the extraction time is short; the extraction is thorough; the extraction method is suitable for industrial application.

The invention discloses a method for producing selenium-rich dendrobium huoshanense by utilizing organic selenium biological agent, comprising the following steps: the biological agent which is rich in organic selenium and prepared by EM (effective microorganisms) probioltics is diluted to the selenium content of 20-30 mg / L, the biological agent is sprayed to the surface of the dendrobium huoshanense plant and culture medium respectively in April and September each year, the spray mount is 40-60 ml / m<2>, the biological agent is sprayed once every 5-7 days, and the biological agent is totally sprayed for 5-6 times. The leaves of dendrobium huoshanense to which the organic selenium biological agent is sprayed is rich in selenium, the dendrobium huoshanense accords with the limit standard of selenium-rich tea in NT / T600-2002 promulgated by the Ministry of Agriculture of China, so that the effects of disease prevention and disease resistance of the dendrobium huoshanense are better played, and the immune function of a human body is improved.

The invention discloses a dendrobium huoshanense tree cultivation method. Dendrobium huoshanense plants are fixed to tree trunks, moss plants are arranged in water to be soaked completely, then 2-3 dendrobium huoshanense plants are taken as a whole plant to be fixed to a bark, the roots of the dendrobium huoshanense plants are wrapped by completely soaked moss and bundled and fixed by using straw ropes, the bundling positions are located at the root positions of the plants and exposed out of the roots to facilitate sprouting, the roots grow along the grains of the trees, the dendrobium huoshanense grow by absorbing nutrients brought by the trees, and finally the roots are embedded into the trees to grow with the trees together. The dendrobium huoshanense are planted on the tree trunks and are fully implanted into the surfaces of the tree trunks, and organic nutrients are produced due to the photosynthesis of leaves and delivered through the barks so that it can be ensured that dendrobium huoshanense planted on the tress are provided with sufficient organic nutrients, the barks are not removed, the trees are protected, and healthy tress growth is ensured.

The invention discloses a preparation method of a dendrobium huoshanense wine for improving human body immunity. The dendrobium huoshanense wine is prepared from distilled spirit taking as base liquor and traditional Chinese medicinal materials, wherein the traditional Chinese medicinal materials comprise dendrobium huoshanense, desertliving cistanche, American ginseng, herb of shorthorned epimedium, barbary wolfberry fruit, prepared rehmannia root, pulp of cornus, eucommia bark, Chinese taxillus twig, oriental waterplantain rhizome, cassia bark, tuber onion seed, hippocamp, cornu cervi pantotrichum and licorice. The preparation method comprises the following steps: crushing the medicinal materials into fine powder of 80-120 meshes, adding 5-8 times of the white spirit of 55-60 degrees, mixing and soaking for 8-12 days, and filtering out wine liquid; and adding 3-5 times of the white spirit of 55-60 degrees, soaking for 6-9 days continuously, filtering out wine liquid, combing the wine liquids, and mixing to an appropriate wine degree to obtain the dendrobium huoshanense wine. The dendrobium huoshanense wine disclosed by the invention has the effects of reinforcing kidney to strengthen yang, replenishing qi to strengthen essence and keeping fit, and has very good curative effects on hyposexuality, cold semen weakness, lassitude in loin and legs, dysphoria insomnia, chronic prostatitis and the like caused by insufficiency of kidney-yang and deficiency of qi and blood.

The invention discloses a preparation method of dendrobium huoshanense pure powder tablets. The method comprises the following steps: (1) picking and selecting dendrobium huoshanense and removing impurities; retaining stems of the dendrobium huoshanense; shearing off fibrous roots of the Dendrobium huoshanense and cleanly washing the dendrobium huoshanense with drinking water; (2) putting the cleaned dendrobium huoshanense in a steam sterilizing cabinet for sterilizing; (3) putting the sterilized dendrobium huoshanense into an ultra-micro pulverizing machine for pulverizing to obtain dendrobium huoshanense juice liquid; (4) adding the pulverized dendrobium huoshanense juice liquid into purified water for dilution and dissolution; sieving, centrifuging, retaining filtrate and removing insoluble substances; (5) carrying out spray drying on centrifugal liquid to obtain dendrobium huoshanense pure powder; (6) tabletting the dried dendrobium huoshanense pure powder by using a tabletting machine to obtain the dendrobium huoshanense pure powder tablets, and then packaging. The preparation method disclosed by the invention has the advantages that a preparation process is simple; a tablet form is adopted, so that the dissolution speed is high and the use is convenient; any auxiliaries are not contained, so that the purity is high, the quality is stable, the availability is high, and the pure powder tablets are safe and effective.

The invention relates to a method for rapid tissue culture propagation of Dendrobium huoshanense seedlings. The seeds and medium of Dendrobium huoshanense are employed to breed seedlings. Breeding of the seedlings consists of the steps of sowing germination and strong seedling culture. The seeds of Dendrobium huoshanense are adopted as the source of seedling breeding, and are acquired from mature Dendrobium huoshanense capsules. In the sowing germination step, the medium is employed to germinate the seeds of Dendrobium huoshanense, the acquired seeds of Dendrobium huoshanense are uniformly sowed in the medium well prepared in advance. The Dendrobium huoshanense's seeds entering the medium absorb nutrients supplied by the medium to make the seeds of Dendrobium huoshanense germinate rapidly. By means of the sowing germination step, the seeds of Dendrobium huoshanense grow into Dendrobium huoshanense seedlings with stems and leaves. The Dendrobium huoshanense seedlings with stems and leaves then grow into plantlets through the strong seedling culture step, thereby forming the seedlings able to be planted out of bottles. The method provided in the invention has the advantages of reasonable design, method optimization, simple operation, and low cost.

The invention discloses a method for planting dendrobium huoshanense at high altitude and belongs to the technical field of plant cultivation. The method specifically includes the following steps of selection of a planting field, soil preparation and seedbed making, preparation of dendrobium huoshanense seedlings, planting, management after planting and harvesting. A high-altitude region serves as the planting field of dendrobium huoshanense, a proper forest branch density is selected, forest land of the high-altitude region is fully used, the original growth state of dendrobium huoshanense is restored, diseases and insect pests hardly exist, and planting does not need to occupy agricultural cultivated land; in the planting process, only silkworm feces is used, no fertilizer is used, ecological environment protection is achieved, and no harm is caused to human bodies. During warm-keeping protection of dendrobium huoshanense seedlings in winter, materials are obtained locally, the dendrobium huoshanense seedlings are covered with broad-leaved forest fallen leaves, an anti-freezing and warm-keeping effect is achieved, the broad-leaved forest fallen leaves are pushed aside in spring of the next year, the fallen leaves are humified and mineralized to form humus, and fertility of a cultivation matrix can be effectively improved.

The invention discloses a detection method of anti-tumor activity of dendrobium huoshanense refined polysaccharide. The detection method comprises an in-vitro anti-tumor detection method and an in-vivo anti-tumor activity detection method; the inhibition rate of the dendrobium huoshanense refined polysaccharide on tumor cells is calculated through the in-vitro anti-tumor detection method; a dose-effect curve is drawn according to different concentration inhibition rates, and the half inhibitory concentration IC50 of the tumor cells is calculated; the in-vivo growth inhibition effect of the dendrobium huoshanense refined polysaccharide on a C57BL / 6J mouse in-vivo transplantable mouse lung cancer LLC tumor is detected through the in-vivo anti-tumor activity detection method; an in-vitro experiment shows that the dendrobium huoshanense refined polysaccharide has an obvious dose effect on an in-vitro anti-tumor effect; an in-vivo activity experiment shows that the dendrobium huoshanense refined polysaccharide has good anti-tumor activity and a relatively good dose-effect relation; the growth of a mouse lung cancer transplantable tumor can be remarkably inhibited and the dendrobium huoshanense refined polysaccharide has an obvious in-vivo anti-tumor effect; two experiment methods are combined so that the detection of the anti-tumor activity of the dendrobium huoshanense refined polysaccharide is more accurate.

The invention discloses a method for planting dendrobium huoshanense seedlings in a cottage mode. The cottage performed in a period from the April to the May, good-quality and strongly growing dendrobium huoshanense is selected, florescent dendrobium huoshanense is inappropriate, 2-3 bud section truncations are reserved upward when dendrobium huoshanense stems are sheared, and notches are smooth. The sheared dendrobium huoshanense stems are disinfected with 400-600 times of liquid carbendazim, soaked for10-15 minutes and then is inserted into a matrix seedbed, grass meal surrounds the peripheries of the inserted stems, and loess covers the grass meal. The upper portions and the lower portions cannot be reversed in the growth direction during inserting. The liquid carbendazim contains 80-120ppm of sodium selenite. The method simulates a soil environment for field growth, meanwhile nutrient elements of soil are increased, dendrobium huoshanense growth and resistance improvement are facilitated, the cottage seedling rate of the dendrobium huoshanense reaches to 95%, the survival rate is greatly improved, and accordingly the seedling production cost is reduced.

The invention discloses a traditional Chinese medical dendrobium huoshanense composition and a preparation method thereof, wherein the traditional Chinese medical dendrobium huoshanense composition is prepared via water or ethanol extraction, concentration and drying of 25-35 percent of dendrobium huoshanense, 20-30 percent of astragalus mongholicus, 20-35 percent of folium cortex eucommiae and 20-30 percent of American ginseng by mass percentage. Based on the traditional Chinese medicine theory, the dendrobium huoshanense and other valuable Chinese herbs are scientifically and reasonably mixed, and the combination and formula are optimized to achieve the purpose of improving healthcare effects.

The invention discloses an underwood planting method for dendrobium huoshanense and belongs to the technical field of plant cultivation. The method comprises the following steps of planting site selection, soil preparation bed arranging, dendrobium huoshanense seedling preparation, planting, planted management and recovery. The proper forest branch density is selected, a forest land of a high altitude area is sufficiently utilized, the original growth situation of the dendrobium huoshanense is recovered, planting can be achieved while not occupying cultivated land in agriculture, and the survival rate of the dendrobium huoshanense reaches up to 95%; due to soaking of seeds through a Chinese herbal medicine solution, no pesticide is applied in the whole planting process, and the edible safety of dendrobium huoshanense is improved; dendrobe seedlings are insulated and protected in winter, local materials are adopted, forest fallen leaves are utilized for covering the materials, the effects of freeze prevention and insulation are achieved, the fallen leaves are pushed aside in early spring of the second year, the fallen leaves are humified and mineralized to form humus, and the fertility of a culture medium can be effectively improved.

The invention discloses a large dendrobium huoshanense seedling planting method. The method includes the following steps of first, seedling selection, second, matrix supply, third, basic field treatment, fourth, planting and fifth, harvesting. Tissue nutrition cultivation is conducted on seedlings firstly, and after the seedlings grow to large seedlings, the large seedlings are transplanted, cultivated and scientifically managed and planted. Harvested dendrobium huoshanense is good in quality, the quality of the dendrobium huoshanense is guaranteed, and the yield of the dendrobium huoshanense is greatly increased.

The invention relates to the field of medicinal plant processing, and provides a deep processing method of dendrobium huoshanense. The method comprises the following steps: (1) preprocessing; (2) extraction; (3) solid-liquid separation; (4) low temperature concentration; (5) continuous vacuum film drying. The invention further provides an application of the deep processing method of the dendrobiumhuoshanense, in particular to the application in the preparation process of dendrobium huoshanense granules, dendrobium huoshanense extract, dendrobium huoshanense wine and dendrobium huoshanense skin care products. The method has the advantages that the whole processing process is low in energy consumption, green and environmentally friendly, the utilization rate of the dendrobium huoshanense can be increased, the processing cost can be substantially reduced, development of the dendrobium huoshanense industry can be greatly promoted in case of popularization, and quite good social and economic benefits are created.

The invention discloses a dendrobium huoshanense facial cleanser which is prepared from the following raw materials in percentage by weight: a dendrobium huoshanense extracting solution, AESA (dodecyl polyoxyethylene ether ammonium sulfate), K12A (dodecyl ammonium sulfate salt), CAB-35 (35% of a cocamidopropyl betaine solution), CHS-35 (35% of a lauryl Hydroxysultaine solution), a pearlescent tablet (ethylene glycol distearate), MAPK (lauryl potassium phosphate salt), citric acid and ethylene diamine tetraacetic acid (EFTA-2Na). The invention discloses a preparation method of the facial cleanser. The produced dendrobium huoshanense facial cleanser, can effectively promote blood circulation, and condition and purify the skin while thoroughly cleaning skin; and the facial cleanser has functions of protecting the skin, removing wrinkle and resisting aging, and can keep the skin pure, fresh and comfortable for long time.

The invention provides a method for improving the alkaloid content of dendrobium huoshanense protocorm, wherein dendrobium huoshanense protocorm is used as a material, and the alkaloid content of the dendrobium huoshanense protocorm is increased by using a Phomopsis elicitor. According to the present invention, the influence of the Phomopsis elicitor on the alkaloid content of the dendrobium huoshanense protocorm during suspension culture is researched, and the purpose of the present invention is to provide the new idea for the large-scale industrial production of alkaloids and other medicinal components from dendrobium huoshanense, and the theoretical basis for the medicine source development of dendrobium huoshanense.

The invention discloses a sanqingshan cold-resistant dendrobium officinale seedling culturing method. The culturing method sequentially comprises the following steps: hybridizing and breeding dendrobium officinale, improving a dendrobium officinale population, breeding high-quality dendrobium officinale variety, and carrying out expanding propagation on high-quality dendrobium officinale seedlings. The group improvement is combined with breeding of high-quality individual plant, the breeding of a high-quality new variety strain and fast propagation of high-quality seedlings by applying plant tissue cell culture technology through adopting the progeny obtained from hybridization of sanqingshan wild dendrobium officinale and dendrobium huoshanense, varieties can be screened out according to cold resistance and content of active ingredients by utilizing low-temperature induction, and the breeding speed of the new strain can be greatly accelerated; and the breeding method has importance values on promoting economic development by forming high-quality dendrobium officinale variety and expanding the resources of the dendrobium officinale seeds.

The invention discloses a seedling growing method for Dendrobium huoshanense. The method comprises the following steps: (1) processing seeds: sterilizing Dendrobium huoshanense seeds, mixing the sterilized seeds with a nutrient matrix, and burying the obtained mixture in a sand pool for 2-3 months; (2) accelerating germination of the seeds: activating the seeds, and using a fermentation solution to carry out constant temperature immersion in order to accelerate the germination; (3) sowing the seeds in a greenhouse: laying a nutrient matrix layer, a plant fertilizer layer and loess to form soilin the planting area of the greenhouse, and sterilizing the soil by the fermentation solution; and (4) regulating the greenhouse: regulating the illumination intensity and the carbon dioxide concentration within and after one month after the sowing. Compared with the traditional methods, the method improves the germination rate by 28.83%, improves the polysaccharide content by 6.66% and improvesthe co of the total alkaloid content by 0.025%; and Dendrobium huoshanense cultivated through has the characteristics of fast growth and strong drug functions.

The invention discloses a compound Dendrobium huoshanense-cistanche-glossy ganoderma buccal tablet and a production process thereof. A formula for the buccal tablet comprises the following raw materials, by weight, 10 to 15 parts of Dendrobium huoshanense, 20 to 25 parts of mulberry, 5 to 10 parts of cistanche, 5 to 10 parts of glossy ganoderma, 10 to 30 parts of a sweetener, 1 to 2.5 parts of Arabic gum, 1 to 5 parts of dextrin and 0.1 to 0.25 part of magnesium stearate. According to the invention, effective extraction and utilization of medical compositions of Dendrobium huoshanense, mulberry, cistanche, glossy ganoderma and the like are realized; dissolution rates of effective components (water-soluble components and alcohol-soluble components of Dendrobium huoshanense, mulberry, cistanche and glossy ganoderma are improved; and the buccal tablet is an instant buccal tablet, is convenient to take and has a good effect.

The invention discloses a method for producing Dendrobium huoshanense alkaloids by cell suspension culture, particularly a method for producing Dendrobium huoshanense alkaloids by performing suspension culture on Dendrobium huoshanense cells by using a cell culture reactor. The method comprises the following steps: (1) unicell preparation; (2) unicell line culture; (3) cell population amplification culture; and (4) Dendrobium huoshanense alkaloid extraction: when the cell density reaches 60000-80000 / ml, collecting the Dendrobium huoshanense cells, lowering the culture temperature to 7-10 DEG C, continuing culturing for 5-7 days, and extracting the Dendrobium huoshanense alkaloids. The method can greatly shorten the cell culture period and enhance the Dendrobium huoshanense alkaloid product yield; and the technique is simple and stable, and is suitable for industrial production.