69results about How to "Rich germplasm resources" patented technology

The invention discloses a novel tuckahoe strain (china center for type culture collection (CCTCC) M2011072) and bag-material efficient cultivation technology. The novel tuckahoe stain is applied to cultivation by adopting the bag-material efficient cultivation technology. The novel tuckahoe stain is mainly characterized in that 1) a mycelium is strong, white, obvious in clamp connection; and hypha is spread quickly in a cultivation process; 2) the tuckahoe fruits earlier, the fruit body is even and strong, the color of pulp is white or light yellow, and the skin of tuckahoe is thin and presents purplish red color; 3)the novel tuckahoe strain has the advantages of being stable inheritable characteristics, strong in anti-bacteria infection capacity, high in biological efficiency and yield, good in quality and the like; and 4) the novel tuckahoe strain remarkably improves content of effective components of amino acid, pachymaran and the like in the tuckahoe.

The invention belongs to a plant breeding method and in particular relates to a breeding method for a high-yield and high-resistance green Chinese onion variety applicable to multi-season cultivation. The breeding method comprises the following processing steps of: breeding crossing parents, carrying out a matching breeding test for cross combination, propagating the parents and propagating a generation F1. According to the breeding method provided by the invention, the problems that varieties are complex, characters of a strain degenerate, individual uniformity is poor, resistance is low and all-year production can not be realized, and the like in the prior art can be solved; the bred green Chinese onion hybrid is high in yielding capability, high in lodging resistance and flooding tolerance and applicable to multi-season cultivation, and disease resistance is obviously enhanced; and application amount of pesticide is reduced, production cost is reduced, land cropping index is increased, environmental protection is facilitated, pollution is reduced, vegetable basket safety is guaranteed, and income is increased.

The invention discloses a method for preparing a cabbage type rape BnPABP3 promoter (PBnPABP3) and the application thereof. The method comprises the following steps of: cloning PBnPABP3 sequence by utilizing the genome walking method, extracting genome DNA by utilizing the SDS cracking method, carrying out enzyme digestion on DNA by respectively using endonuclease DraI, EcoRV, PvuII and StuI in different systems, carrying out the first round of PCR amplification by adopting the product as a template after joint segments are connected with the product, and carrying out the second round of PCR amplification by adopting the 50 times diluent of PCR products obtained in the first round as a template so as to obtain the PBnPABP3. The PBnPABP3 is applied to anther and pollen grains of plant. The promoter has the function of driving exogenous gene expression, the expression parts of the promoter are in the anther and pollen grains of plant, and the promoter has the application potentiality in the aspects of improving the safety of rape edible oil as well as the quality of crops, artificially creating sterile line and restorer line, enriching germ plasm resources, and the like.

The invention relates to a successful cutting propagation method of desert caragana, which comprises the following steps of: selecting a strongly growing bush as a maternal plant, and respectively scissoring a twig, a hard branch and a taproot of the desert caragana for cutting; putting the twig of the desert caragana into 100ppm of growth regulator solution, wherein the soaking depth is 2-3 centimeters, and the soaking time is 6.5-10 hours; putting the hard branch into 500ppm of growth regulator solution, wherein the soaking depth is 2-3 centimeters, and the soaking time is 1-3 hours; soaking the cutting of the taproot in 50ppm of growth regulator solution for 18-20 hours; and selecting a position with flat land and good drainage for arranging a cutting bed, selecting sandy soil as a cutting ground mass, cutting at the depth of 4-5 centimeters, watering after cutting, setting up a shading net in time, watering once every five days, tearing off the shading net after one month, and counting the survival rate after three months. An experiment result indicates that the processing method can ensure the success of the cutting propagation of the desert caragana, and provide abundant germ plasm resources for ecological restoration and stockbreeding production in desert regions.

The invention discloses a method for restoring cadmium pollution soil in an ecological mode through turfgrass, and relates to the technical field of heavy metal pollution soil ecological restoring. The method comprises the steps of (1) cadmium-resisting Bermuda grass screening, (2) ecological restoration of cadmium pollution soil and (3) maintenance and management. According to the method, the cadmium-resisting Bermuda grass screened through graded-concentration cadmium stress treatment is used for restoring the cadmium pollution soil in the ecological mode, the bare earth surface can be quickly covered, the ecological afforestation problem of cadmium pollution soil is effectively solved, the purpose of continuously extracting and removing cadmium in soil can be achieved through grass cutting, and the method is easy to popularize and apply, meets the afforestation requirement, effectively lowers cadmium face source pollution, and has good social benefits and environment benefits.

The invention relates to the field of cross breeding of plants and particularly relates to an interspecific hybrid embryo rescuing and seedling forming method of sweet cherries in southern China and Chinese cherries. The method combines advantages of European sweet cherries and the Chinese cherries, and takes the 'European sweet cherries' as a female parent and the 'Chinese cherries' as a male parent to carry out interspecific distant hybridization. Aiming at the abortion problem of a distant hybrid embryo, the hybrid embryo is subjected to embryo rescuing. A proper sampling period of the embryo rescuing of each hybrid combination, concentrations and ratios of respective exogenous growth regulators for hybrid young embryo germination, subculture propagation and rooting culture, and a proper acclimatization and transplanting method are determined. By applying an interspecific hybrid embryo rescuing system of the sweet cherries in southern China and the Chinese cherries, which is established by the technology, the embryo germination rate reaches 80.00 percent, the multiplication coefficient of subculture hybridized new shoots reaches 5.0, the rooting rate of rooting culture is 90.91percent, the growth vigor of embryo culture seedlings is good and the seedlings grow robustly and have green leaves, and the acclimatization and transplanting survival rate reaches 77.27 percent.

The invention provides a tissue culture and propagation method of viburnum tinus. The tissue culture and propagation method comprises the following steps: selection and treatment of explants: selecting stem segments with axillary buds of viburnum tinus as culture materials, rinsing with water, performing surface sterilization with alcohol on a super clean bench, rinsing with sterile water, sterilizing with mercuric chloride, rinsing with sterile water, and inoculating in an induction medium; induction culture: taking an MS medium to be mixed with 6-benzylamino adenine, naphthylacetic acid, sucrose and agar, using the mixture I as the induction medium, and inoculating the selected stem segments with axillary buds of viburnum tinus in the induction medium; proliferation culture: transferring the explants growing in the induction medium to the induction medium formed by mixing the MS medium with 6-benzylamino adenine, naphthylacetic acid, sucrose and agar, and performing continuous culture; rooting culture: selecting 1 / 2 MS medium to be mixed with indolebutyric acid, activated carbon, sucrose and agar, and using the mixtureII as a rooting medium; transplanting: taking out the rooted plants, and transplanting into a mixed medium containing humus soil, cinders and sawdust.

The invention discloses a brassica napus BnPABP8 promoter and a preparation method and use thereof. The preparation method comprises the following: A, a step of primer design, which is to design specific walking primers according to known sequences; B, a step of brassica napus promoter preparation, which is to extract brassica napus DNA by using a sodium dodecyl sulfonate (SDS) lysis method, perform genome walking according to genome walking kits, digest 5 micro liters of DNA by using DraI enzyme, detect the purity of the DNA, perform enzyme digestion of the DNA by using the incision enzymes provided by four kits, and purify the product of the enzyme digestion; C, a step of purification, which is to add ethanol into enzyme digestion solution, precipitate, centrifuge, dry in the air, dissolve in sterile water and add specific connectors after purification is accomplished, wherein GSP1 and AP1 are used as primers in primary polymerase chain reaction (PCR) reaction; and D, a step, which is to perform amplification by using the diluted solution of the product of the primary PCR reaction as a template and GSP2 and AP2 as primers in a secondary PCR reaction, recover purified product of the secondary PCR product, connect with a T vector, and obtain the PBnPABP8. The invention also discloses the use of the PBnPABP8 in roots, stems, leaves, buds and anthers of plants. When the promoter is used, the safety of the edible brassica napus oil can be improved, and the disease resistance, stress resistance and lodging resistance in crops can be improved by transgenosis.

The invention discloses a breeding method of sterile and maintainer lines of north glutinous sorghum. The breeding method comprises the following specific steps: 1, hybridizing by taking non-glutinous sorghum LgB / R5M874B of a maintainer line as a female parent and a glutinous sorghum maintainer line 45B as a male parent, and inbreeding generations by selecting single plants with three good characters of glutinousness, large spikes and early mature; 2, hybridizing by taking a non-glutinous sorghum sterile line A2V4A as a female parent and an F2 generation in the step one as a male parent, carrying out four-generation backcross transformation by adopting a method of carrying out stable hybridization together with backcross transformation and taking good single plants of a filial generation as a male parent, and breeding a glutinous sorghum sterile line by taking good sterility, glutinous grains and heat smut resistance as breeding standards, wherein in glutinousness identification in the step one or step two, only endosperm is cut for carrying out iodine solution reaction, so that normal sprouting of the glutinous grains is ensured, and embryo is prevented from being damaged due to length cutting of the grains. According to the breeding method disclosed by the invention, good characters of yield, quality, resistance, combining ability, appearance and the like of south and north sorghum are organically integrated, so that the sterile and maintainer lines can become basic parent lines of the north glutinous sorghum, and the germplasm resource of the glutinous sorghum is enriched.

The invention discloses a nectarine planting method, which relates to a cultivation method of fruit trees and belongs to the technical field of crop cultivation. The cultivation method is realized by the fact that plentiful nutrients are supplied to nectarine trees, so as to shorten the bearing time, increase the bearing quantity, reduce the fruit cracking, enhance the fruit quality and sense and conveniently manage the fruit trees. The cultivation method has the beneficial effects that the nectarine cultivation technique of the province is improved, germplasm resources are enriched, standardized production is realized, the production purposes of early production, high yield and good quality are achieved, tree bodies are fully exposed to light, fruits have the advantages of high sugar degree, good taste and high quality, and the trees are in small sizes and can be conveniently managed. The cultivation method has the advantages of simplicity, convenience in operation, time saving, labor saving and good economic benefit and has great popularization value.

The invention discloses a preparation method and an application of a brassica napus BnPABP5-3 promoter (PBnPABP5-3). A genome walking method is utilized to clone a PBnPABP5-3 sequence: an SDS (sodium dodecyl sulphate) cracking process is applied to extract genome DNA; in different systems, endonucleases DraI, EcoRV, PvuII and StuI are used to digest DNA, and the digested DNA is respectively connected with joint segments, then primary PCR (Polymerase Chain Reaction) amplification is carried out by taking the DNA connected with the joint segments as templates, and secondary PCR amplification is carried out by taking 50-time diluted solution of products of the primary PCR as a template, thus the PBnPABP5-3 is obtained. The PBnPABP5-3 can be applied to the anther, pollen grain, root tip and ovule of a plant. The promoter has the function of driving expression of a foreign gene, the expression part is in the anther, pollen grain, root tip and ovule of the plant, and the promoter has application potential in the improvement of the safety of rape edible oil, the improvement of the stress resistance and lodging resistance, improvement of the quality of crops, artificial sterile line creation, germ plasma resource enrichment and the like.

The invention discloses a method for preparing a promoter (PBnPABP2) of Brassica napus BnPABP2 and the application thereof. The operation of cloning a PBnPABP2 sequence by using a genome walking method comprises the following steps: extracting genome DNA by using a SDS cracking process; in different systems, carrying out restriction endonuclease reaction on the extracted DNA respectively by using endonucleases DraI, EcoRV, PvuII and StuI; after the obtained DNA is connected with a linker fragment, carrying out first-round PCR amplification by taking the linker fragment as a template; and carrying out second-round PCR amplification by taking a50-time diluent of the obtained first-round PCR product as a template, thereby obtaining the PBnPABP2. The PBnPABP2 is applied in the roots, stems, leaves, flower buds, anthers and peels of plants. The promoter has the function of driving the expression of exogenous genes, and the expression sites thereof are respectively in the roots, stems, leaves, flower buds, anthers and peels of plants; and the promoter has application potentials in the aspect of safety of edible oil of Brassica napus and the aspects of improving the disease resistance, stress resistance and lodging resistance of crops, improving the quality of crops, artificially creating a male sterile line and a restoring line, enriching germ plasm resources, and the like through transgenosis.

The invention discloses a sanqingshan cold-resistant dendrobium officinale seedling culturing method. The culturing method sequentially comprises the following steps: hybridizing and breeding dendrobium officinale, improving a dendrobium officinale population, breeding high-quality dendrobium officinale variety, and carrying out expanding propagation on high-quality dendrobium officinale seedlings. The group improvement is combined with breeding of high-quality individual plant, the breeding of a high-quality new variety strain and fast propagation of high-quality seedlings by applying plant tissue cell culture technology through adopting the progeny obtained from hybridization of sanqingshan wild dendrobium officinale and dendrobium huoshanense, varieties can be screened out according to cold resistance and content of active ingredients by utilizing low-temperature induction, and the breeding speed of the new strain can be greatly accelerated; and the breeding method has importance values on promoting economic development by forming high-quality dendrobium officinale variety and expanding the resources of the dendrobium officinale seeds.

The invention relates to a method for acquiring a peanut mutant by ion beam mutagenesis. The method includes: using ion beams of different doses to irradiate different species of peanut embryos; subjecting the irradiated peanut embryos to water culture germination experiment, and subjecting M1 generation to field sowing; after harvesting of the M1 generation, treating and sowing M2 generation according to different species and different doses to obtain M3 generation peanut seeds; selecting the same number of field overground part plants from different species of mutagenesis groups, conducting single plant harvesting, performing airing, and then determining the fat content and fatty acid content so as to obtain a mutant. The method provided by the invention has the advantages that by means of ion beam mutagenesis, ion beam irradiation mutagenic treatment is carried out on different species of peanut embryos. A large number of experimental data researches show that ion beams enable peanuts to produce various mutants, but the effects are different among different species; mutant peanuts show traits different from normal plants at different growth and development stages, thus being conducive to selection and identification of peanut breeding.

The invention discloses a brassica napus BnCP51 promoter as well as a preparation method and an application of the promoter. The preparation method comprises the following steps of: performing polymerase chain reaction (PCR) on the basis of a primer designed according to a BnCP51 gene upstream 2kb sequence obtained from the all genomic sequencing of brassica napus, thus obtaining the sequence of a 5' upstream promoter of the brassica napus BnCP51; and designing a primer according to the 5' upstream 2kb sequence of the BnCP51 obtained from the all genomic sequencing of the brassica napus, extracting genomic DNA of brassica napus leaves, performing PCR amplification on the genomic DNA, connecting the genomic DNA with a pMD18-T carrier after performing purification and recovery, transformingcompetent cells, picking positive clone, and sequencing after PCR positive detection and digestion verification, thus obtaining the upstream 2kb flanking sequence of target genes, namely PBnCP51. Dueto the efficient expression of the PBnCP51 in rape anther and pollen grains, the expression efficiency of foreign genes in transgenic plants is improved, thus the PBnCP51 can be used in the gene engineering study of the plants and in the transgenic safety study of seed rape.

The invention discloses a method for performing sterilization by using a mixed antibacterial agent in the cultivation process of chlamydomonas reinhardtii and belongs to a method for removing bacteria and fungi from chlamydomonas reinhardtii. The method comprises the following steps of: A. inoculating mixed infectious microbes polluted chlamydomonas on a TAP solid medium plate and cultivating for 3 to 5 days under the conditions of 23 + / - 0.5 DEG C, 14 / 10 hour light / dark period and light intensity of 8000 Lx; B. preparing a mixed antibacterial agent of azoxystrobin of 10 mu g / ml and nalidixic acid of 15 mu g / ml for later use on the TAP solid medium plate; C. transferring and lining the cultivated infectious microbe-containing chlamydomonas to the TAP plate containing the mixed antibacterial agent and cultivating for 5 to 8 days under the conditions of 23 + / - 0.5 DEG C, 14 / 10 hour light / dark period and light intensity of 8000 Lx; and D. transferring and lining the microbe-removed chlamydomonas to the common TAP solid medium plate again, cultivating for 3 to 5 days under the conditions of 23 + / - 0.5 DEG C, 14 / 10 hour light / dark period and light intensity of 8000 Lx, and performing subsequent experiments or performing conservation for standby application. The method has the advantage that fungi are removed by azoxystrobin and bacteria are removed by nalidixic acid, so that the problem about infectious microbe pollution in scientific experiments and application process of the chlamydomonas reinhardtii is solved.

The invention belongs to methods for killing bacteria and fungi in chlamydomonas reinhardtii and discloses a method for sterilization in a chlamydomonas reinhardtii culture process by mixture of tebuconazole and nalidixic acid. The method includes: A, inoculating chlamydomonas contaminated by mixed bacteria onto a TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in an occulting light period of 14 / 10 and under the light intensity of 8000Lx; C, transferring the chlamydomonas well grown on the TAP solid medium plate to a TAP plate with mixed antibacterial agents, and culturing for 5-8 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx; D, transferring the sterilized chlamydomonas to the common TAP solid medium plate, and culturing for 3-5 days at the temperature of 23+ / -0.5 DEG C, in the occulting light period of 14 / 10 and under the light intensity of 8000Lx to achieve a standby for subsequent experiments or breed conservation. The method has the advantage that the problem of bacterial contamination in scientific experiment and application process of chlamydomonas is solved by adoption of the mixed antibacterial agents for treating bacterial and fungus contamination of chlamydomonas.

The invention discloses an inducing method of gracilaria verrucosa callus. The method is characterized by comprising the steps of inoculating gracilaria verrucosa thallus which is adopted as an explant and sterilized by a detergent and mercury bichloride in an inducing culture medium, and adding 2mg of 2,4-D, 1mg of IAA, 1mg of KT and 30g of cane sugar in a 1L PESI culture medium; inducing the explant for 7d in darkness, and continuously culturing the explant under the illumination condition to form a great amount of callus. By adopting the method, the callus can be successfully induced by adopting the gracilaria verrucosa as the explant, the induction rate of the callus can reach 82 percent, the callus can further develop to form bud or thallus of the gracilaria verrucosa, and not only can a great amount of gracilaria verrucosa thallus be formed, but also rich seed resource can be provided for the gracilaria verrucosa.

The invention belongs to the field of molecular markers and provides a rapid identification method of a self-compatible Si molecular marker, a self-compatible marker primer and a self-compatible variety of loquat. The Si molecular marker is shown in a sequence SEQ ID NO. 1, and the size is 830bp; an AS-PCR (allele-specific polymerase chain reaction) sequence of the marker primer is that a forward primer is shown in SEQ ID NO. 2, and a reverse primer is shown in SEQ ID NO. 3. According to the identification method, by using Si as the molecular marker, DNAs of different loquat varieties are amplified by using the AS-PCR of the marker primer; the DNAs are screened and the DNAS where 830bp fragment can be amplified have the Si molecular marker, which shows that the loquat variety is the self-compatible variety. The method can quickly identify the self-compatible variety of loquat without arranging a pollinate tree or manual pollination, so that the cost can be greatly lowered; the method is hardly affected by external factors, is great in precision, breaks the limitation of conventional method, and has great significance on the yield and quality of loquat.

The invention discloses a cultivation method of a stress-resistant broccoli new variety. The method includes: building callus regeneration systems of different sources; building a broccoli loose embryonic callus induction system; screening an appropriate mutagenesis method; building an efficient regeneration system, and acquiring a regeneration culture medium; acquiring a callus growth culture medium with screening pressure; screening resistant calluses; inducing regenerated buds to obtain regenerated seedlings; further breeding individual plants survived under the screening pressure, and transplanting to a natural environment to perform further selective culture to obtain ideal stress-resistant mutants so as to obtain the stress-resistant broccoli new variety. The method has the advantages a foundation is laid for the cultivation of high-quality vegetable varieties with disease resistance, drought resistance, cold resistance and the like during production, chemical fertilizer use reduction and ecological system restoration are promoted, a new approach is provided for vegetable stress-resistant breeding, vegetable seed resources are enriched, and a foundation is laid for safety vegetable production.

The invention discloses a method for breeding high-yield high-quality stress-resistant good new common andrographis herb varieties. The method includes the steps: taking common andrographis herb seedsas raw materials, taking 60 Co-gamma rays as radiation sources, radiating the common andrographis herb seeds, dividing the radiated seeds into three groups, soaking the seeds by distilled water, germinating the seeds in the salt stress environments containing NaCl solution and the drought stress environments containing PEG 6000 solution, and calculating germination rate, germination potential, germination indexes, vigor indexes, root length and bud length or fresh weight; selecting new salt-tolerant and drought-resistant high-yield high-quality stress-resistant varieties according to germination rate, germination potential, germination indexes, vigor indexes, root length and bud length or fresh weight of each sample. According to the method, the common andrographis herb seeds are radiatedby the aid of suitable dosages of 60 Co-gamma rays, good tolerance improvement methods of common andrographis herbs under salt stress and drought stress are expected to be achieved in practice, and application potential is high. According to the method, injury of adverse situations to plants is relieved, and the method has high application values.

The invention discloses an induction method of gulfweed regeneration plant. The induction method of the gulfweed regeneration plant comprises the following steps: preparing a sterile explant, inoculating the explant to an induction culture medium and inducing callus to form at an induction temperature of 15-20 DEG C with a light intensity of 4000-5000Lx and a light period of 8 hours; processing gulfweed rhizoid in 0.2% povidone for 3 minutes, and then sterilizing in a 3% sodium hypochlorite solution for 5 minutes, sterilizing in 0.1% mercury bichloride for 3 minutes so as to obtain a sterile survival explant; adding 0.1-1mg of ZT, 1-2mg of IAA (Indole Acetic Acid), 1-3g of ascorbic acid, 30g of cane sugar and 7g of agar in a 1LPES culture medium, sterilizing at a high temperature and a high pressure for 10-20 minutes to bud the gulfweed.

The invention belongs to the field of distant hybridization breeding methods for plants, and discloses a method for cultivating a corn-tripsacum monosomic addition line by using a corn allopolyploid. The method comprises the following steps: hybridizing the corn allohexaploid (Tripsazea creammaize T.; 2n=76) serving as a nonrecurrent parent with a corn (Zea mays L.) serving as a recurrent parent, then carrying out distinguishing and selection of the number of chromosomes and chromosome compositions on a fructification progeny, and finally obtaining the corn-tripsacum monosomic addition line with total 21 chromosomes including 20 corn chromosomes and 1 tripsacum chromosome. According to the method, an effective way for introducing high-quality germplasm of tripsacum into the corn is supplied; furthermore, the monosomic addition line supplies good materials for researches on a gene function; the monosomic addition line is high in stability and can be stably transmitted to the progeny. The method is simple, short in time and high in efficiency.

The invention discloses a wolfiporia cocos YX1 which is preserved in the China Center for Type Culture Collection (CCTCC) with the preservation number of CCTCC NO: M2021434. The invention further discloses a stock culture medium and a mother culture medium of the wolfiporia cocos YX1. The invention further discloses a cultivation method of the wolfiporia cocos YX1. The wolfiporia cocos YX1 is screened out, and is stable in economic character, high in yield, excellent in quality and stable in genetic gene; a high-yield and stable-yield culture medium of the wolfiporia cocos YX1 is screened out; and the scientific and practical cultivation method is provided.

The invention discloses a BnALS1 mutant gene and protein based on gene editing and application of the BnALS1 mutant gene and protein. The invention also discloses a cytosine base editing vector. The invention reports that single base editing is realized in rape for the first time. A base editing method provided by the invention can directionally improve rape traits. Compared with chemical mutagenesis, the method is faster, more efficient and more accurate. Meanwhile, germplasm resources of the rape resisting herbicides are enriched. Mutant materials R10 and R144 obtained in the invention havestrong tribenuron-methyl herbicide resistance. 20 days after 20 ml of 15 mg ai / L tribenuron-methyl is sprayed to each plant in the 5-6 leaf stage (approximately equivalent to 5 times of a recommendedamount for broadleaf weed prevention and control), the R10 and R144 materials are not affected at all, and transgenic receptor materials of the rape suffer from severe phytotoxicity (new leaves wither, and old leaves stop growing and turn purple).

The invention discloses a Chinese herbal medicine plant cultivation system and device with a hyperlipidemia prevention and treatment effect. The aim is to provide the indoor plant cultivation system for assisting growth of Chinese herbal medicine plants through microorganisms (beneficial flora) and a Chinese herbal medicine plant cultivation device of the system. According to the system, the compatibility of the beneficial flora and Chinese herbal medicine is achieved through special matching, the effect of effectively preventing and treating hyperlipidemia can be achieved, symbiosis of the beneficial flora is achieved, and the promotion effect on growth of the Chinese herbal medicine plants can also be achieved. Meanwhile, the established system can also purify indoor air and beatify an indoor environment, the indoor vertical space is sufficiently used, the cost is low, and the beneficial effect is significant.

The invention relates to a method for obtaining tartary buckwheat seeds easy to shell and belongs to the technical field of genetic seed breeding of crops. According to the provided method, a chemicalmutagenesis method is adopted to obtain the high-quality tartary buckwheat seeds. The adopted technical scheme includes the steps that a, ethylmethane sulfonate is utilized to process 5,000 ethylmethane sulfonate (Heifeng No.1) seeds, and the seeds are soaked in an EMS solution for 8-10 hours and stirred several times during soaking; then, the solution is poured out carefully, sodium thiosulfateis adopted for neutralizing the EMS solution, distilled water is adopted for flushing several times, and the seeds are placed in a fuming cupboard for airing for preparation of sowing; b, the processed seeds are sown in land which is ploughed previously, wherein if the statistic fatality rate of the sown seeds reaches 30-50%, mutagenesis is effective; c, M1-generation mutants are obtained from each single plant in order to harvest potential available mutants; d, after screening and seed baking, it is found out that shells of seeds of three single plants are broken, after continuous planting ofM2-M5 generations, three plant systems with stable gene expression are obtained, and more than 100 single plants are obtained. The method has significant meaning on diversification of tartary buckwheat seed resources, shortening of a traditional seed breeding process and boosting of tartary buckwheat seed breeding work.

The invention discloses an anti-fatigue health-care product and a preparation method thereof. The anti-fatigue health-care product comprises the following raw materials: malt, Chinese dates, radix astragali, Chinese wolfberry fruits, Chinese yams, mint, green tea and additives, wherein the additives are pine nodular branches and nardostachys roots, and the additives are chrysanthemums and white funguses. The preparation method of the anti-fatigue health-care product comprises the following steps of selecting materials, performing cleaning, performing pretreatment on the raw materials, then decocting the raw materials for the first time, performing filtration so as to obtain a first-time medicine liquid, then performing decoction once to twice, mixing the medicine liquids, performing decoction, performing filtration, performing concentration to obtain clear paste, adding an appropriate amount of maltose or honey, and performing decoction so as to obtain finished products. According to the anti-fatigue health-care product and the preparation method thereof, the anti-fatigue health-care product is prepared based on the guidance of the theory of traditional Chinese medicines, main components are selected from medicinal and edible medicinal materials, and the anti-fatigue health-care product is free from toxic and side effects, wide in application crowds, quick to take effect, goodin effects, simple to prepare, mild in medicine properties and suitable for long-term taking, has favorable treatment and relief effects on fatigue caused by various factors, and concurrently has theefficacy of strengthening and consolidating body resistance.