Sanqingshan cold-resistant dendrobium officinale seedling culturing method

Abstract

The invention discloses a sanqingshan cold-resistant dendrobium officinale seedling culturing method. The culturing method sequentially comprises the following steps: hybridizing and breeding dendrobium officinale, improving a dendrobium officinale population, breeding high-quality dendrobium officinale variety, and carrying out expanding propagation on high-quality dendrobium officinale seedlings. The group improvement is combined with breeding of high-quality individual plant, the breeding of a high-quality new variety strain and fast propagation of high-quality seedlings by applying plant tissue cell culture technology through adopting the progeny obtained from hybridization of sanqingshan wild dendrobium officinale and dendrobium huoshanense, varieties can be screened out according to cold resistance and content of active ingredients by utilizing low-temperature induction, and the breeding speed of the new strain can be greatly accelerated; and the breeding method has importance values on promoting economic development by forming high-quality dendrobium officinale variety and expanding the resources of the dendrobium officinale seeds.

Description

technical field [0001] The invention relates to a method for cultivating cold-resistant dendrobium officinale seedlings in Sanqing Mountains. Background technique [0002] Dendrobium officinale Kimura et Migo is a perennial epiphytic herb of the genus Dendrobium orchidaceae, also known as black knot grass, which is a precious medicinal plant. Dao Zang is known as "the first of the nine Chinese celestial grasses", and the folks call it "life-saving celestial grass". Dry polydipsia, deficiency of stomach yin, lack of food and retching, persistent deficiency and fever after illness, hyperactivity of fire due to deficiency of yin, fatigue and heat due to bone steaming, dark eyesight, weakness of muscles and bones; modern research shows that Dendrobium officinale contains polysaccharides, alkaloids, amino acids and other ingredients can significantly improve the immune function of the body, and have the effects of nourishing the stomach, nourishing the kidneys, nourishing yin an...

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Problems solved by technology

[0006] At present, scientific research units and enterprises in Zhejiang, Guangdong, Yunnan and other provinces have carried out artificial breeding research on Dendrobium officinale, and have bred Xiandrobium No. 1, Xiandou No. 2, Zhongke No. 1, Hongxin No. 1, etc. New varieties (lines), these new varieties (lines) are all full-sibling or half-sibling families, that is, after selfing or hybridization of specific new plants, and then using their capsules (seeds) to reproduce to form the next generation of seedling populations, and The main purpose of breeding these new varieties (lines) is high yield and high quality, and there is no public report on the research on resistance

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