The invention discloses a broad-spectrum and high-efficiency method for extracting RNA from various plant tissues and belongs to the technical field of biochemistry. In the invention, boric acid buffer solution is used as a buffer system, the plant tissue RNA is effectively separated and enriched first, the mixture of tris(hydroxymethyl)aminomethane (tris), hydrochloric acid (HCl), ethylenediamine tetraacetic acid disodium salt (EDTA-Na2) and sodium chloride (NaCl) is used as extraction buffer solution, hexadecyl trimethyl ammonium bromide (CTAB) is used as a detergent, 1 mol / L guanidinium isothiocyanate is used as a strong denaturant for inhibiting the activity of RNase, and thus high-quality RNA is extracted. The method can extract RNA from tissues such as ovules, anthers, blades and fibers of cotton and can extract high-quality RNA from recalcitrant plants such as arabidopsis thaliana, rice, draba matangensis, banana (peel and meat), masson pine and deodar. When the method is used for extracting RNA, the yield is high, the purity is high, and salt pollutant is avoided.