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Production method for fluorescent microscopic slices of ovule of pollinated azalea

A production method, the technology of Rhododendron, is applied in the direction of fluorescence/phosphorescence, preparation of test samples, material excitation analysis, etc., which can solve the problems of difficult compression of Rhododendron ovary, inability to obtain fertilization information, and ovule structure damage, etc., and achieve reliable The effect of data and technical support

Active Publication Date: 2015-02-18
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Description
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the disadvantages of the existing method of pollination style fluorescent micrographs that Rhododendron ovary is difficult to compress, ovules cannot be seen clearly or ovule structure is destroyed so that comprehensive fertilization information cannot be obtained, and its purpose is to provide Fluorescent microscopic tableting method for rhododendron pollination ovules with good tableting effect, clearly visible ovules, and clearly visible fertilization process

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  • Production method for fluorescent microscopic slices of ovule of pollinated azalea
  • Production method for fluorescent microscopic slices of ovule of pollinated azalea

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Embodiment Construction

[0017] The invention provides a method for making fluorescent microscopic slices of rhododendron pollinated ovules, which consists of five steps of sampling and fixing rhododendron pistils after pollination, softening, dyeing, peeling off the ovary wall, and making slices. Each step is specific. The operation is as follows:

[0018] (1) Sampling and fixation: pick off the pollinated rhododendron flowers, peel off the petals, and leave only the pistil including the style and ovary as the material to be tested and fix it in FAA fixative solution, and transfer to the volume fraction after 24 hours For preservation in 70% ethanol solution, the FAA fixative is formed by mixing 70% ethanol, 38% formaldehyde and glacial acetic acid by volume fraction, and the volume of 70% ethanol: glacial acetic acid: 38% formaldehyde The ratio is 8:1:1;

[0019] (2) Heating and softening treatment: wash the material to be tested stored in the 70% ethanol solution once with water, put it in a 10ml ...

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Abstract

The invention provides a production method for fluorescent microscopic slices of ovule of pollinated azalea. The production method comprises the following steps: sampling pistil of pollinated azalea; and fixing, softening, dyeing, stripping ovary walls and tabletting. In the steps of sampling and fixing, pollinated azalea flowers are picked, flower petals are stripped, only pistil parts comprising stigmas and ovaries are remained and are put into an FAA fixing solution to be fixed, and after 24 hours, the pistil parts are converted into a 70% ethanol solution to be stored; in the softening process, the fixed materials are put into a drying oven to be heated and softened; in the dyeing process, the softened materials are dyed with a 0.1% water-soluble aniline blue dyeing solution without light for one night; in the ovary wall stripping process, the dyed materials are subjected to ovary wall stripping treatment; and in the tabletting process, tabletting is performed on the stripped materials to obtain the slices. According to the method, the slices of ovule of pollinated azalea, which are clearly dyed and have complete tissue structure, can be obtained; and comprehensive fertilization information can be observed.

Description

technical field [0001] The invention relates to a method for making fluorescent microscopic slices, in particular to a method for making fluorescent microscopic slices of rhododendron pollinated ovules. Background technique [0002] Rhododendron occupies an important position in the world's gardens, and there is a saying in Europe that "no garden can't be completed without Rhododendron". Compared with developed countries in Europe and the United States, where there are many varieties of azaleas and are widely used, the varieties of azaleas cultivated and applied in landscaping in my country are relatively single, and mainly imported foreign varieties. my country is one of the main distribution centers of Rhododendron plants in the world. Among the wild species, there are many species with unique flower color, flower shape and fragrance. It is a breakthrough to cultivate new varieties of Rhododendron with independent intellectual property rights in my country. [0003] Our c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N21/64
Inventor 解玮佳李世峰王继华彭绿春李树发蔡艳飞宋杰陆琳张露
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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