High-efficient breeding technique of Eurasian raisin grape

Abstract

The present invention relates to a high-efficient breeding technique of Eurasian raisin grape. After the raisin grape has flowered for 30-50d, the young fruit of the grape is picked. The ovule is taken out in a sterilized state. The embryo is inoculated to an MS culture medium containing different levels of plant growth regulators of gibberellic acid (GA3), indoleacetic acid (IAA) and 6-benzylaminopurine (6-BA) for executing embryo rescue. The average embryo development rate using the breeding technique of the invention is increased from 8.3-11.9% of a patient disclosed in the background art to 84%. The germination rate obtains 75.33% and the seedling rate obtains 74.17%. Furthermore the embryo rescue technique of the raisin grape obtained through the technique of the invention has the advantages of increased seedling rate of offspring, easier obtainment of more filial generation, increased parent selection range of raisin grape cross breeding, accelerated breeding process of new species of the raisin grape and increased breeding efficiency of the raisin grape through increasing the embryo development rate and the germination rate of the raisin grape.

Description

technical field [0001] The invention relates to a high-efficiency breeding technology for Eurasian seedless grapes, belonging to the technical field of plant breeding. Background technique [0002] 1. Publication No. CN 101238791 A. Title "A Technology for Improving Seedless Grape Breeding Efficiency", which is prepared by dissolving a plant growth inhibitor substance chlormequat or a cytokinin substance 6-BA The medicament is sprayed on the flower spikes 10-20 days before flowering, the grape fruit grains are picked 40-50 days after flowering, and the ovules are taken out for embryo rescue. The embryo development rate of the seedless white of the present invention is reduced from 0 to 4.4%. Increased to 8.3-11.9%; Moritani Seedless increased from 0.5-2.5% to 1.8-21.9%; Flame Seedless increased from 7.1-17.1% to 14.1-25.4%. [0003] 2. The zygotic embryos of seedless grapes are aborted during the development process and cannot develop into normal seeds. Therefore, conventio...

AI Technical Summary

Problems solved by technology

[0003] 2. The zygotic embryos of seedless grapes are aborted during the development process and cannot develop into normal seeds. Therefore, conventional breeding can only use seedless varieties as male parents to crossbreed seedless grape varieties, but the probability of seedless grapes in the offspring is low ( 0~15.9%), low breeding efficiency

In terms of the sampling time of immature embryos, if the collection time is too early, the immature embryos are too small to survive; if the collection time is too late, the immature embryos have already died, and the purpose of cultivation cannot be achieved.