692 results about "Indoleacetic Acids" patented technology

The invention discloses a plant growth regulating composition and its application. The composition comprises the following components: A) natural abscisic acid, B) gibberellic acid (GA3, or of GA4 +7), and C) an existing plant growth regulator. The existing plant growth regulator is selected from one or more than two of 6-BA (6-benzylaminopurine), thidiazuron, brassinolide, sodium nitrophenolate, potassium nitrophenolate, chlormequat chloride, triacontanol, naphthlcetic acid and its salt, indolebutyric acid and its salt, indoleacetic acid and its salt, ethephon, diethyl aminoethyl hexanoate, paclobutrazol, uniconazole, fulvic acid, Mepiquat chloride, nitrohumic acid, jasmonic acid and triapenthenol. Specifically, the components A), B) and C) are in a weight ratio of 1:0.001-500:0.001-500. The S-ABA (S-abscisic acid) added in the invention can expand the prevention and treatment objects, reduce or prevent phytotoxicity, reduce dosage, and lower the use cost.

The invention relates to a plant nutrient liquid fertilizer. In order to solve the problem of over fertilization, the plant nutrient liquid fertilizer is prepared from the following raw materials in proportion by weight: aqua brassin 12-36, gibberellin 1-4, indoleacetic acid 30-80, abscisic acid 10-20, naphthaleneacetic acid 50-90, salicylic acid 80-180, vitamin C 1-3, cane sugar 40-100, chitin 20-80, 0.05-0.2 percent triacontanol 400-1,000, hydrolyzed protein 2-5, potassium dihydrogen phosphate 300-400, thiourea 40-120, cobalt chloride 5-12, borax 50-100, sodium silicate 30-55, urea 300-500, ammonium molybdate 50-80, copper sulfate 30-65, zinc sulfate 120-200, magnesium sulfate 100-150, calcium chloride 50-120, manganese sulfate 40-80 and water in the amount of 3-8 times the total amount of all the raw materials or water in the amount capable of dissolving all the raw materials, wherein the water is de-ionized water or micro cluster water; and the preparation method is that all the materials are prepared into aqueous solution. The plant nutrient liquid fertilizer has the advantages of favorably solving the problems of over fertilization and shortage of nutrient elements in soil, obviously improving the plant adverse resistance, improving the photosynthesis and the accumulation efficiency of nutritive materials and improving the yield and the quality.

The invention relates to the technical field of fruit growing and in particular relates to a growth regulator for improving the fruit setting rate of peaches and a regulating method. The growth regulator comprises the following components of gibberellin and auxin, wherein the auxin may be one of 1-Naphthalene acetic acid, sodium alpha-naphthyl acetate, indoleacetic acid, phenylacetic acid, indolebutyric acid, indoleacetic acid and 2,4-dichlorphenoxyacetic acid, and the growth regulator is sprayed after the peaches bloom. The growth regulator provided by the invention is easy to obtain and prepare and low in economic cost, and can effectively remedy deficiency of endogenous hormone caused by poor fertilization of peaches; the method for applying the growth regulator provided by the invention is simple and convenient and easy to operate and control, effectively solves the problem that in the growing of peaches, the fruit setting rate is influenced by abnormal weather in a flowering phase, shriveled flower buds, overly rapid warming and the like, improves the fruit setting rate by more than 34 times, and is obvious in effect and applicable to popularization and application in the production of peaches in common peach orchards, green houses and other facilities.

The invention discloses a bacillus megaterium strain X3 and application thereof to plant growth promotion. The bacillus megaterium strain X3 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 11, 2015 with the preservation number of CGMCC No.10803, is obtained through separation of indoleacetic acid, has the characteristics of siderophore production, phosphate solubilizing, ammonia producing, salt tolerance and disease suppression, can remarkably promote crop growth and improve corn yield, and meanwhile, can increase the diversity and the abundance of microbes in soil. The bacillus megaterium strain X3 is quick in reproduction, simple in production process, strong in adversity ability, easy to preserve and conductive to industrial production, and can not only prevent and cure soil-borne diseases but also promote plant growth when utilized to prepare a bio-fertilizer, thereby having a wide application prospect.

The invention discloses bacillus megaterium and an application thereof, and belongs to the field of microorganisms. A plant growth promoting bacterium (bacillus megaterium JX15) is collected in the China General Microbiological Culture Collection Center on Dec.20, 2011, the class name is bacillus megaterium and the collection number is CGMCC No.5622. The bacillus megaterium has the beneficial effects that a strain can produce indoleacetic acid with high yield, can be used for nitrogen fixation and can grow by using sparingly-soluble phosphates as phosphorous sources, so as to improve the utilization rates of fertilizers, promote plant root development and absorption of the fertilizers, and increases the contents of soil mineral nitrogen and available phosphorus; the strain has a good effect on promoting growth of peanuts; the high-yield indoleacetic acid promotes the growth and the development of the peanuts; and the nitrogen and phosphate fertilizer utilization rates of the peanuts are relatively high due to the increasing of the contents of the soil mineral nitrogen and the available phosphorus.

The invention relates to acinetobacter calcoaceticus X.L.Zhu.SGD07-3-16 with free living nitrogen fixation capability. The acinetobacter calcoaceticus is preserved in China general microbiological culture collection center, and the preservation number of the acinetobacter calcoaceticus is CGMCCNo.5455. The acinetobacter calcoaceticus is high in nitrogenase activity and phosphorus and potassium dissolving capability, and has ACC (1-aminocyclopropane-1-carboxylic acid) deaminase activity, a certain amount of indoleacetic acid can be generated, and the generation of siderophore is avoided; when an acinetobacter calcoaceticus-containing liquid or solid microbial agent is used for inoculating crops such as corns, oilseed rapes and leaf mustard, the growth of the crops can be remarkably promoted, and the yield of the crops can be increased.

The invention relates to a method for the early fruition and luxuriant production of fruit trees, in particular to a method for early fruition and luxuriant production of American thin-shell kiskatoms. The technical proposal of the invention is as follows: cions of American thin-shell kiskatoms of a second year are grafted in parental stocks of American thin-shell kiskatoms of the last year; a part of soil is then dug out to expose partial root of the parental stock for crab rooting cultivation that adopts a growth regulator; the growth regulator used by each strain of American thin-shell kiskatom plantlet is 0.18mg rhodofix and 0.12mg indoleacetic acid; the use method is that mixed liquid is made and mixed by adding 200g water in the rhodofix for dissolving and adding 200g water in the indoleacetic acid for dissolving; the mixed liquid is sprayed at the exposed root of each strain of American thin-shell kiskatom plantlet. The method of the invention has the effect of early fruition and luxuriant production. The mixed growth regulator by adopting the rhodofix and indoleacetic acid can further improve the effect of early fruition and luxuriant production.

The invention relates to a seaweed fertilizer comprising a seaweed leach liquor. The seaweed fertilizer is prepared by the following steps: (1) seaweed extraction; (2) preparation of a fermentation medium; (3) inoculation and fermentation. The seaweed fertilizer provided by the invention is an active biological fungicide fertilizer of seaweed produced by pure physical and biological methods, and has the activity of seaweed and the living body adjusting function of biological fungicide. The number of effective living bacteria is greater than or equal to 2.0*10 to the power of 9 CFU / ml. Meanwhile, the fertilizer is rich in plant growth-regulating activity substances such as alginic acid, seaweed oligosaccharide, gibberellin (GA) and indoleacetic acid (IAA) biologically fermented, degraded and converted from seaweed gel. The fertilizer is directly absorbed by root systems of crops conveniently and fast in fertilizer efficiency, and can effectively accelerate high yield and early-ripe of plants, enhance the disease-resistance and improve the quality of crops, so that the fertilizer is an excellent special fertilizer for organic food.

The invention provides bacillus velezensis for inhibiting virus and promoting plant growth and application of a bacillus velezensis SNB55 strain in prevention and treatment of plant virus diseases andplant fungal diseases and promotion of plant growth. The bacillus velezensis for inhibiting virus and promoting plant growth and the application of the bacillus velezensis have the beneficial effectsthat the bacillus velezensis has no toxicity and pathogenicity, is safe to human and livestock, is environment-friendly, and has high stress resistance and stable antibacterial activity; the bacillusvelezensis can inhibit phytopathogens such as bacillus cereus, tobacco mosaic virus, tomato yellow leaf curl virus, fusarium wilt of cucumbers, tobacco target spot pathogens, phytophthora capsici, rice sheath blight disease and botrytis cinerea, can be secrete indoleacetic acid to promote seed germination and plant growth; and the strain is a multifunctional biocontrol bacterium and has a wide application prospect.

The invention provides a rapid propagation technology of Paris polyphylla Smith. The method comprises the following steps of: culturing tissues of the Paris polyphylla Smith such as rhizome sections and the like on a murashige and skoog (MS) culture medium, exactly taking a rhizome section with 1 to 2 buds as an explant, disinfecting, inoculating the explant into an MS plus 0.5mg / L 6-benzylamino adenine (BA) plus 0.4mg / L naphthylacetic acid (NAA) culture medium for culturing, ensuring the germination of the buds within about 6 to 8 days after the inoculation and the formation of seedlings with leaves within about 10 days, and cutting the seedlings and inoculating the seedlings into an MS plus 1.5mg / L 6-BA plus 0.4mg / L indoleacetic acid (IAA) culture medium to ensure that the high-speed increase is maintained and the multiplication factor is more than 5. MS plus 1.5mg / L 6-BA plus0.4mg / L IAA plus 0.5mg / L NAA is selected as a rooting culture medium, the rooting rate is over 90 percent, and an intact plant can be formed within about 25 days. A test tube seedling is transplanted into sand, the water retention and moisture retention are noticed, the transplant survival rate is over 90 percent after the seedling is transplanted for 1 month, and a set of high-frequency stable regeneration system is established. The method has the advantages of high stability, simple operation, high propagation speed, low production cost, industrialization level and the like.

The invention discloses a method for preparing vegetation concrete greening additive AB bacteria, belonging to the field of ecological restoration of high and steep rocky side slope and aiming at solving the problem in vegetation concrete ecological slope protection technology that the substrate has a bad structure and is easy to harden so as to inhibit seeds of plants from sprouting and growing due to the addition of cement. The vegetation concrete greening additive AB bacteria comprises peanut shell, powdery coal cinder, peat soil, calcium superphosphate, compound fertilizer, urea, carboxymethyl cellulose, agar powder, polyacrylamide, brassinolide, naphthylacetic acid, indoleacetic acid, compound sodium nitrophenolate, gibberellin, and triacontanol, and is prepared in multiple steps. The additive is added while the vegetation concrete substrate is sprayed, so that beneficial microbial communities are increased, the transparent commutativity of the substrate is improved, the buffer capacity of rhizospheric environment is improved, and the growing activity of the plant is improved.

The invention discloses an Azorhizobium strain SCAUs152 and application thereof. The strain is a new strain which is separated and purified from fresh soybean root nodules and belongs to Rhizobium. The strain is collected to China Center for Type Culture Collection of Wuhan University on March 14th, 2014, and the collection number is CCTCC NO.M2014085. The strain is applied to production of Sichuan soybeans. The Azorhizobium strain SCAUs152 is a favorable broad-spectrum Rhizobium japonicum strain with high symbiotic fixation capacity, wide application range of Sichuan soybean species, IAA (indoleacetic acid) secretion capacity, capacities for dissolving inorganic phosphorus, organic phosphorus and potassium and high stress tolerance. The strain has favorable matching affinity with Sichuan dominant bean species, and can enhance the soybean yield by more than 23% in different ecological regions where no nitrogen fertilizer is applied and the Azorhizobium strain SCAUs152 is inoculated, which is much different from the regions where the Azorhizobium strain SCAUs152 is not inoculated.

The invention discloses a seedling raising method for oil-used peony seeds. The method comprises the steps of (1) selecting seeds; (2) soaking the seeds for disinfecting; (3) treating the oil-used peony seeds of step (2) by using cold plasma generated by atmospheric dielectric barrier discharge for 50-60 seconds; (4) preparing a treatment solution containing 0.25mg / L of gibberellin, 2mg / L of indoleacetic acid and 3mg / L of calcium chloride; (5) performing variable temperature stratification: mixing the oil-used peony seeds treated in step (3) with fine sand, perlite and vermiculite in a ratio of 1:(3-5):(1-2):(1-2), adjusting the humidity to 40-50%, and performing variable temperature stratification in a constant temperature incubator according to three stages; (6) sowing; and (7) performing seedling management. By adopting the seedling raising method for the oil-used peony seeds, seed dormancy can be effectively broken during breeding, so that seeds are quickly germinated, germination of seeds is accelerated, the emergence rate of seeds is improved, the emergence time is shortened, the emergence is even, and the growth indexes of young seedlings such as fresh weight, plant height, root system and the like are improved to different degrees; and the method does not have pollution to the environment.

The invention discloses a peanut seed coating additive which consists of the following components in parts by weight: 15-20 parts of Tianda 2116, 3-6 parts of brassinolide, 4-7 parts of gibberellin, 2-5 parts of kinetin, 3-6 parts of 6-benayl aminopurine, 4-7 parts of naphthylacetic acid, 4-6 parts of indoleacetic acid, 3-5 parts of salicylic acid, 2-4 parts of triacontanol, 10-14 parts of amino-oligosaccharin and 2-3 parts of methyl cellulose. According to the optimum mixture ratio of plant growth regulators to physiological activators, the seed germination speed, the germination speed and the seedling growth speed are obviously increased, full, neat and strong seedlings are facilitated, a solid foundation is laid for the final yield of peanuts, the germination of mixed peanut seeds is ahead of time by 2.5-3.8 days, the emergence rate is increased by 4.1-5.4 percent, the content of dry matters in the seedling stage is increased by 13.3-25.0 percent, and the yield of peanut pods is increased by 12.7-21.4 percent.

The invention discloses a method for promoting the accumulation of oil in microalgae and keeping the high biomass of the microalgae. The method is concretely characterized in that enrichment-cultured microalgae are cultured in a nitrogen-deficient medium containing a plant hormone. The plant hormone indoleacetic acid is added to the nitrogen-deficient medium for the first time in order to stimulate the accumulation of oil in microalgae and keep the high biomass of the microalgae, so the microalgae can still keep a high biomass under a nitrogen deficiency condition. Test proves that the method makes the oil content of chlorella higher than the oil content of the chlorella cultured in a nitrogen-free medium free from the plant hormone, so the plant hormone can be used as a good means for stimulating the microalgae to produce oil. The method has the advantages of simplicity in operation, short time, high efficiency, easiness in realization, effective increase of the biomass concentration of the microalgae under a nitrogen-deficient culture condition, and good applicability, and provides an effective technical scheme for microalgae to produce biodiesel or develop other biomass energy sources.

The invention relates to indole carbazole and bisindole maleimide alkaloid, a preparation method and application thereof. In the preparation method, the compound of the type with a novel structure is prepared by a chemical reaction starting from indole and indoleacetic acid. An experiment confirms that the compound has tumor cytotoxin activeness and protein kinase C(PKC) inhibitory activity, so the compound can be used as an cell proliferation inhibitor or an antitumor agent.

The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an ASPAT (Asparatate AminoTransferase) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding an ASPAT polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides hitherto unknown ASPAT-encoding nucleic acids and constructs comprising the same, useful in performing the methods of the invention. Furthermore, the present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by increasing expression in a plant of a nucleic acid sequence encoding a MYB91 like transcription factor (MYB91 ) polypeptide. The present invention also concerns plants having increased expression of a nucleic acid sequence encoding an MYB91 polypeptide, which plants have increased yield- related traits relative to control plants. The invention additionally relates to nucleic acid sequences, nucleic acid constructs, vectors and plants containing said nucleic acid sequences. Even furthermore, the present invention relates generally to the field of molecular biology and concerns a method for improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GASA (Gibberellic Acid-Stimulated Arabidopsis). The present invention also concerns plants having modulated expression of a nucleic acid encoding a GASA, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention. Yet furthermore, the present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important yield-related traits in plants. More specifically, the present invention concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding an AUX / IAA (auxin / indoleacetic acid) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding IAA polypeptide, which plants have enhanced yield-related traits relative to control plants. The invention also provides constructs comprising AUX / IAA-encoding nucleic acids, useful in performing the methods of the invention.

The invention relates to a plant growth regulation composition containing plant activator protein. The composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is selected from the plant activator protein; the active ingredient B is selected from any one of compounds as follows: benzyl aminopurine, brassinolide, indoleacetic acid, harpin protein and gibberellic acid. The composition is mainly used for increasing the plant chlorophyll content, keeping plants green, preventing plant senescence, promoting root development and effective absorption of crops on fertilizers, assisting in good growth of weak parts of the crops, enhancing the absorption of the plants on water and the fertilizers and regulating the balance of the water in the plants so as to improve the drought resistance and the cold resistance of the plants.

The plant growth regulating composition contains natural abscisic acid, 4-chloroindoleacetic acid or indoleacetic acid and naphthylacetic acid as active coponents except common inert processing assistant. If necessary, it may also contains 8-hydroxyquinoline.

The invention discloses a tissue culture and propagation method and inductive culture mediums for Chongzuo camellia nitidissima. According to the method, the improved MS, 6-benayl aminopurine (6-BA) and 3-indoleacetic acid (IAA) are used as an inductive culture medium for the cluster buds of Chongzuo camellia nitidissima; and the combination of the two growth hormones can be used for activating acting enzymes in the body cells of Chongzuo camellia nitidissima, and promoting the germination and the growth of the explants of Chongzuo camellia nitidissima in a proper concentration and a weakly acidic environment; the thick and strong cluster buds can be formed after the culturing for 55 days, and the propagation rate is increased by 20 times. According to the method disclosed by the invention, 1 / 2 improved MS and indolebutyric acid (IBA) or 1 / 2 improved MS and napthaleneacetic acid (NAA) are further used as an inductive culture medium for rooting, and capable of effectively promoting the single seedlings of Chongzuo camellia nitidissima to root to form new plants. Via the tissue culture and propagation method applying the three inductive culture mediums for Chongzuo camellia nitidissima, Chongzuo camellia nitidissima can be rapidly and effectively propagated, thus solving the problems that wild resources are diminished gradually and the natural propagation rate of Chongzuo camellia nitidissima is low, and effectively protecting the precious plant genetic resource which is rare in the world.

The invention provides Bacillus velezensis K01 and application thereof. The Bacillus velezensis K01 has the growth promotion functions of dissolving phosphorus and generating indoleacetic acid, cellulase, protease and the like, also can generate anti-bacteria active substances and volatile anti-bacteria substances, can inhibit growth of various pathogenic bacteria, has a good biocontrol function, also can favorably realize colonization on the rhizosphere of a plant and in the plant, can be applied to plant growth promotion and biological control of plant diseases, and has a good application prospect.

The invention discloses a disease-prevention and growth-promoting Trichoderma harzianum and an application thereof. The strain number of the Trichoderma harzianum is TM2-4, which is registered in theGeneral Microbiology Center of China Microbial Culture Collection Management Committee as CGMCC No. 15881. The Trichoderma harzianum M2-4 can inhibit Botrytis cinerea, Prunus persica, Colletotrichum gloeosporioides and Fusarium oxysporum f. Sp. Cucumerinum on fruits and vegetables, and has good control effect on tomato gray mold in vitro and can promote the germination of tomato seeds and the growth of tomato plants. The Trichoderma harzianum TM2-4 has indoleacetic acid (IAA)-producing activity, ferrophilic activity, glucanase and chitinase-producing activity. The Trichoderma harzianum TM2-4 has high breeding speed and can be cultured artificially, and the culture condition is simple, and the spore production is up to 3.2 * 109 cfu / g on the solid fermentation medium of wheat bran and corncob nutrient solution.

Plant-growth-promoting bio-organic fertilizer is prepared by inoculating 0.5-2% (w / w) of composite fermentation microbial agent to livestock-poultry manure and auxiliary material, fermenting, inoculating 2-5% (w / w) of plant-growth-promoting microbial agent and fermenting. A preparation method includes steps: (1), preparing a fermenting material; (2), performing high-temperature aerobic fermentation; (3), preparing the plant-growth-promoting microbial agent; (4), after-composting organic fertilizer. The plant-growth-promoting bio-organic fertilizer and the preparation method have the advantages that rapid proliferation of plant-growth-promoting functional microorganisms is realized in the process of fermenting of two times, and effective biological viable bacteria count of the organic fertilizer reaches 5x108CFU / g; composting degree is high, the organic fertilizer can generate various plant hormones including 3-indoleacetic acid, cytokinin, gibberellins and ethylene after being applied in soil, can promote plant growth and can increase yield of crops and incoming of farmers; the bio-organic fertilizer prepared by the method is low in cost, environment-friendly and high in efficiency.

The present invention relates to a high-efficient breeding technique of Eurasian raisin grape. After the raisin grape has flowered for 30-50d, the young fruit of the grape is picked. The ovule is taken out in a sterilized state. The embryo is inoculated to an MS culture medium containing different levels of plant growth regulators of gibberellic acid (GA3), indoleacetic acid (IAA) and 6-benzylaminopurine (6-BA) for executing embryo rescue. The average embryo development rate using the breeding technique of the invention is increased from 8.3-11.9% of a patient disclosed in the background art to 84%. The germination rate obtains 75.33% and the seedling rate obtains 74.17%. Furthermore the embryo rescue technique of the raisin grape obtained through the technique of the invention has the advantages of increased seedling rate of offspring, easier obtainment of more filial generation, increased parent selection range of raisin grape cross breeding, accelerated breeding process of new species of the raisin grape and increased breeding efficiency of the raisin grape through increasing the embryo development rate and the germination rate of the raisin grape.

The invention belongs to the field of wheat genetic breeding, and provides major QTL (Quantitative Trait Loci) capable of influencing the wheat ear length. The major QTL are located on a 2D (Two-Dimensional) chromosome short arm, and are located between a left marker AX-108988107 and a right marker AX-111096297 of an SNP (Single Nucleotide Polymorphism) marker; the major QTL can affect the spikelet number at the same time; the QTL have a candidate gene for encoding IAA (Indoleacetic Acid)-amino acid hydrolase; phenotypic variation explanation rates of the QTL on the ear length and the grain number per ear are respectively 27.9 to 20.1 percent and 18.5 to 10.2 percent; positive allelic variation of the QTL are sourced from parent varieties-Banmangzi, and the QTL have huge application valuein wheat breeding.

The invention relates to a determination method for six residual plant-growth regulators in a bean sprout, especially to a method for simultaneous determination of six residual plant-growth regulators in the bean sprout by using gas chromatography-tandem mass spectrometry. Six plant-growth regulators involved in the invention are composed of 4-chlorophenoxyacetic acid, 2,4-dichlorophenoxyacetic acid, naphthylacetic acid, indoleacetic acid, 2,4-D-butyl ester and indolebutyric acid. The method comprises the following steps: 1) sample treatment; 2) determination, wherein gas chromatography-tandem mass spectrometry is employed; 3) drafting of a standard curve; 4) blank test; and 5) result calculation and description. The determination method employs gas chromatography-tandem mass spectrometry for simultaneous determination of the six residual plant-growth regulators in bean sprout; and the method is simple, convenient, rapid, accurate and reliable, and the lower determination limit, recovery rate and precision of the method all accord with requirements.

The invention discloses pseudomonas vranovensis with phosphorus solubilizing capability, a preparation method and application thereof. Through an ultraviolet-ray plasma composite mutation technology for multiple composite mutation, a bacterial strain ZSW-3-5-1a with high solubilizing efficiency on phosphorus and good stability is selected and bred, has the classification name of pseudomonas vranovensis, is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 8750. The bacterial strain has efficient solubilizing capability on calcium phosphate and calcium phytate, and also is capable of generating a certain amount of indoleacetic acid. By using a liquid containing the bacterium or a solid inocula to inoculate corn, soybean, mustard and other crops, utilization rate on phosphorus in soil is improved and crop output is improved.

The invention discloses an alfalfa growth-promoting rhizobacteria MJM-11 and application thereof. An Enterobacter ludwigii strain MJM-11 has a preservation number of CGMCC No.6295. The Enterobacter ludwigii strain MJM-11 can be applied in at least one of the following four ways: 1, preparation of an ACC (1-aminocyclo propane-1-carboxylate) ammonialyase; 2, preparation of indoleacetic acid; 3, preparation of siderophores; and 4, promotion of the growth of plants under the saline-alkali stress. Experiments prove that the alfalfa growth-promoting rhizobacteria MJM-11 which is obtained through separation can be used for synthesizing the ACC ammonialyase and the IAA (Indole Acetic Acid) siderophores and is capable of effectively promoting the alimentation of plants in a saline-alkali stress environment, regulating the growth of plants and enhancing the stress resistance capacity of plants in adverse conditions.

The invention discloses Ensiferadhaerens CGMCC 6315 which is a bacterium having a nitrogen-fixing capability. The strain is also a plant growth promoting rhizobacterium (PGPR), and can produce indoleacetic acid (IAA), extracellular polysaccharide (EPS), siderophore, salicylic acid (SA), 2,3-dihydroxybenzoic acid (DHBA), hydrogen cyanide (HCN) and ammonia. Under the stress condition of a salt containing sodium chloride, soybeans inoculated with the E.adhaerens CGMCC 6315 can have a higher germination rate in comparison with a control group which is not inoculated with the strain.

The invention relates to a DCPTA-containing (2-(3,4-dichlorophenoxy)triethylamine containing) plant growth regulation composition, comprising active ingredient A and active ingredient B; the active ingredient A is DCPTA; the active ingredient B is selected from any one of brassinolide, gibberellic acid, 2-diethylaminoethyl hexanoate, indoleacetic acid, harpin Ea, jasmonic acid, methyl jasmonate, and allantoin; the DCPTA-containing plant growth regulation composition is mainly used for increasing activity of plant endonuclear, increasing the contents of chlorophyll, protein, nucleic acid, juice, oil and lipoid in plants, maintaining green and preventing aging, significantly enhancing photosynthetic effect in crops, promoting development of roots, promoting effective absorption of fertilizer by crops, increasing yield and income, assisting in good growth of subordinate parts of crops, enhancing water-fertilizer absorption by plants, increasing the accumulative storage of proteins, lipids and the like, and promoting cell division and growth.