306 results about "Methyl jasmonate" patented technology

Lipo-chito oligosaccharides (LCOs) are produced by culturing rhizobacteria cells in or on a culture medium comprising at least one of: jasmonic acid or a derivative thereof; linoleic acid or a derivative thereof; or linolenic acid or a derivative thereof. Preferably, the rhizobacteria cells are Bradyrhizobium japonicum cells having the identifying characteristics of B. japonicum strain USDA 3. Preferably, the derivative of jasmonic acid is an ester thereof, preferably methyl jasmonate. Also provided are methods for improving LCO production at low temperatures, particularly temperatures below 25° C.

Described is a bioprocess for the high-yield production of food flavor-acceptable jasmonic acid and methyl jasmonate, as well as a novel jasmonic acid isomer produced thereby and organoleptic uses thereof. The process yields at least 5% of the "cis" isomer defined according the structure:(wherein R is hydrogen or methyl) or at least 5% of the "cis" isomer defined according to the structure:(wherein R is hydrogen or methyl). Compositions containing at least 98% of the isomer having the structure:with an optical rotation (alphaD20) of +58° are novel. Compositions containing at least 98% of the isomer having the structure:with an optical rotation (alphaD20) of +58° are also novel. The process of our invention comprises the cultivation under aerobic condition of one or more specific strain of Diplodia gossypina in a nutrient medium followed either by (1) isolation of the jasmonic acid product or (2) esterification of the jasmonic acid to form methyl jasmonate followed by the isolation of the methyl jasmonate and novel products produced by such process.

Personal care composition comprising at least one hair growth regulating compound selected from the group consisting of glyceryl dilaurate, apigenin, tetrahydrocurcumin, oleanolic acid, azelaic acid, sulforaphane, canavanine, pyridoxal 5-phosphate, phytic acid, tannic acid, grape seed extract, NG-nitro-L-arginine-methyl ester, benzamidine, sodium butyrate, betulinic acid, polyornithine, polyarginine, fisetin, jasmonates, methyl-jasmonate, cis-jasmone, caffeic acid phenethyl ester, delphinidin, ethyl abietate, esculetin, sorbic acid methyl ester, canaline, N-formyl-methionine, N-formyl-alanine, taurine, palmitoyl carnitine, undecanol, undecylenic acid, rutin, fusidic acid, phenyl pyruvic acid, L-isoleucine, phenyl glycine, silibinin, silymarin, L-ascorbic acid-6-palmitate, N-undecylenoyl-L-phenylalanine, and salts, derivatives and mixtures of any of the foregoing; and a dermatologically-acceptable carrier.

The invention relates to the field of biotechnology and discloses an agilawood production method. The method comprises the steps of first drilling a hole in a trunk of aquilaria malaccensis or aquilaria sinensis, enabling an agilawood inducer formed by plant hormones, inorganic salt and phenethyl alcohol to be added dropwise into the tree in a transfusion mode, then sealing the hole, adding the agilawood inducer dropwise at an interval of 1-2 months, performing treatment for 3-24 months in total, then cutting brown oily matter and yellowish-brown discolored xylem in the trunk, and obtaining agilawood after drying in the sun. The plant hormones are one or more than two of methyl jasmonate, ethephon and 6-BA; and the inorganic salt is one or both of sodium chloride and magnesium chloride. Components of the agilawood inducer are adjusted on the basis of an existing agilawood inducer, a trunk defense system is excited, related enzymes for synthesizing agilawood chemical components are generated and act on phenethyl alcohol, the trunk generates related chemical components in the agilawood, and finally the agilawood is formed by accumulation. According to the method, not only are contents of agilawood oil and sesquiterpenes in the agilawood oil improved, but also contents of alcohol-soluble extracts are improved.

The invention relates to a natural anti-staling agent suitable for the storage and the fresh keeping of mangos and a preparation method thereof. The natural anti-staling agent consists of eugenol or clove oil, methyl jasmonate, CaCL2, a solvent, water and a surfactant, wherein the mass volume ratio of the eugenol or the clove oil to the methyl jasmonate to the CaCL2 to the solvent to the surfactant to the water is 5-20:0.5-2.0:0.5-2.0:10-30:0.1-0.4:70-230. The preparation method comprises the following steps of: dissolving the methyl jasmonate in an organic solvent at the room temperature or in a water bath between 25 and 35 DEG C to prepare a methyl jasmonate solution; adding a proper quantity of the eugenol or the clove oil into the methyl jasmonate solution, and stirring and mixing the mixture evenly; then adding the CaCL2 into a small amount of the water until the CaCL2 is dissolved completely; adding the surfactant into water to prepare an emulsion of which the volume ratio is 0.5 to 1.0 percent; and finally, adding the emulsion into the methyl jasmonate solution added with the eugenol or the clove oil and into the CaCL2 solution to 100 percent, and mixing the two solutions to obtain the natural anti-staling agent. The natural anti-staling agent has the functions of fresh keeping and sterilization at the same time, and the mangos treated by the natural anti-staling agent has obviously relieved diseases, and the product appearance and the quality are obviously improved.

The present invention discloses a kind of diazosulfide new compound structure and its application for promoting plant cell secondary metabolic product growth. Said invention selects and uses taxus chinensis cell as application system, and proceeds from structure of immunoactivating agent thiobiazole to design and synthesize new type plant cell inductive activating agent. The test results show that the capacity of said invented benzothiobiazole compound for promoting production of taxane is similar to or higher than that of existent methyl jasmonate.

The method of preparing low molecular polyamide by china wood oil relates to a different method to prepare epoxide resin curing agent. Firstly, china wood oil and methyl alcohol have ester interchange with the inorganic alkali or Louis catalyst which is 0:1%-5% of china wood oil, generating methyl jasmonate, then have addition reaction of methyl jasmonate it and unsaturated binary acid with Louis, generating which finally has amidation with polybasic amine in 150-230deg.C; then clear away the material did not react, acquiring low molecular polyamide. The invention has no pollution to the environment, low energy cost, the epoxide resin and its solified product have good resistance to heat and high mechanical strength.

The invention discloses an artificial cultivation method for increasing content of an active ingredient in roots, stems and blades of red-rooted sage. In the method disclosed by the invention, a step of spraying a mixed aqueous solution of methyl salicylate and methyl jasmonate is only added on the basis of the original conventional production and planting process, and the sprayed aqueous solution is prepared by adding 0.01-0.25g of chemically pure or industrially pure methyl salicylate and 0.015-0.40g of methyl jasmonate into 100L of water and then stirring. The method is simple to implement, the investment is less, the harvest is great, the total content of salvianolic acid ingredient in a red-rooted sage plant can be greatly increased, specifically, the total content of the salvianolicacid ingredient is increased to about 10% from about 6% and is nearly twice of the original total content of the salvianolic acid ingredient, especially the content of the salvianolic acid ingredientin the stems and blades of the red-rooted sage plant can be greatly increased, specifically, the total content of the salvianolic acid ingredients is increased to about 5% from about 1.6% and is nearly more than four times of the original total content of the salvianolic acid ingredient, thus the stems and blades of the red-rooted sage also have values in development and utilization.

The invention discloses a gene promoter of ginseng PgPDR3 responded by methyl jasmonate and an application thereof. The sequences of the gene promoter of the ginseng PgPDR3 are shown in SEQ ID NO.1, or are the DNA sequences of which the homology with SEQ ID NO.1 is over 99%. According to the invention, a Genomewalker technology is adopted to clone, later the sequences of the gene promoter of the PDR transport protein is obtained, and then the expression vector Pcambia1301-ProPDR3::GUS of the promoter is built; if the promoter in the transgenic tobacco drives the GUS to express, and can be controlled by ginsenoside, salicylic acid, gibberellins and abscisic acid, the promoter participates in the transport and accumulation of the ginsenoside, which provides a basis for cloning the gene of key control recording factor in the ginsenoside transport signal pathway and controlling the transport and accumulation of the terpenoid, such as ginsenoside; and fine plants containing rich ginsenoside can be obtained by using a method for controlling or improving the ginseng and other plants via the promoter or the derivative thereof.

The invention discloses a balance growth regulator for a plant. The balance growth regulator is characterized by comprising a filling agent and at least four crop growth regulators selected from brassinolide, methyl jasmonate, auxin (heteroauxin), salicylic acid, cytokinin, gibberellic acid, abscisic acid, ethylene and triacontanol. The product disclosed by the invention is a plant growth regulator prepared from at least more than four traditional endogenous plant growth regulators, the balance growth regulator not only can be used for greatly increasing the yield, but also can be used for additionally producing dry matters, shows favorable crop disease and disadvantage resistance and is green, safe and incapable of injuring eaters.

The invention relates to an application method of an elicitor in the hairy root of American ginseng, belonging to the field of crop biotechnology. The process comprises the following steps: (1) induction of the hairy root of American ginseng; (2) measurement of the growth dynamic and ginsenoside content of the asexual system of the hairy root of American ginseng; (3) optimization of culture conditions for the hairy root of American ginseng; (4) preparation of an elicitor; (5) regulation of enzyme activity in a metabolic pathway. In the method provided by the invention, the combination of the culture conditions for the hairy root of American ginseng is put forward, the influence of two elicitors, namely salicylic acid and methyl jasmonate, on the metabolism of American ginseng is studied in order to increase the saponin output, a basis is provided for large-scale production of American ginseng saponin, and a foundation is laid for realizing industrial production of American ginseng saponin so as to meet the ever-increasing needs of people.

The invention provides a method for producing astaxanthin by haematococcus pluvialis induced by methyl jasmonate, which comprises the following steps: (1) culturing methyl jasmonate cells to exponential phase so as to obtain cultured microalgal liquid for induction; and (2) accumulation of astaxanthin: adding methyl jasmonate concentrate the mass concentration of which is 95 percent into cultured microalgal liquid until the final concentration of the methyl jasmonate is 4.95 to 5.05 mg / L; then culturing the microalgal liquid added with methyl jasmonate until the haematococcus pluvialis cells are completely red under an optical microscope at a temperature of 25 to 28 DEG C and an optical intensity of 4500 to 5000lx and under the conditions of continuous illumination of fluorescent lamps and nutritive salt starved compound stresses so as to finish the accumulation of astaxanthin, wherein at the moment of finishing the accumulation of astaxanthin, the maximum output of the astaxanthin is reached. The invention is simple, easy to operate and low in cost, can greatly shorten the time that the haematococcus pluvialis cells are completely red, and significantly increase the output of the astaxanthin, thereby greatly improving the efficiency of producing astaxanthin by haematococcus pluvialis.

The present invention relates to a plant growth regulator composition, and is characterized by that in addition to the convertional inert processing adjuvant it contains the following active components: A). natural abscisic acid; B), nicotinamide; and C). at least one plant growth regulating material selected from jasmonic acid, methyl jasmonate or brassinolide.

The invention discloses a biological corrosion preventing and freshness-keeping method for oranges and tangerines, and the method sequentially comprises the following steps:1) adjusting the concentration of methyl jasmonate in a sealed container to 95-105mumol / L, and putting oranges and tangerines into the sealed container so as to be steamed for 22-26 hours at the room temperature; 2) taking out the oranges and tangerines processed by the step 1, and then naturally ventilating the oranges and tangerines; 3) immersing the ventilated oranges and tangerines into a 1*10<10>-1*10<12> cell / L cryptococcus laurentii suspension for 1-3 minutes, and then taking out the oranges and tangerines and drying by virtue of natural ventilation.

The invention discloses a turpentine collection method, a lipotrophic agent used in the method in a matching manner and a method for preparing the lipotrophic agent. The collection method comprises the following steps of: pre-treating a pine tree to be subjected to turpentine collection by spraying 0.05 percent methyl jasmonate; formally cutting and applying the lipotrophic agent; subsequently, cutting and applying the lipotrophic agent every other 15 days until the turpentine collection period in the current year comes to an end; and continuously cutting on the upper edge of the cutting plane of the last year for the collection cutting of the next year. The lipotrophic agent comprises the following components: 18 to 33 parts of 98 percent concentrated sulphuric acid, 150 to 135 parts of water, 25 parts of 40 percent ethephon, 4 parts of vaseline, 2 parts of AE (Ethyl Alcohol Polyoxyethylene Ether), 1 part of CDHT (Cetyl Alcohol), 75 to 65 parts of white carbon black, and 75 to 65 parts of glutinous rice flour. The method for preparing the lipotrophic agent comprises the following steps of: diluting the concentrated sulphuric acid; adding the ethephon reagent; adding the vaseline,the AE and the CDHT; and adding the white carbon black and the glutinous rice flour. According to the invention, the advantages of small cut plane load rate, low cutting frequency, prolonged collection cutting age limit of a single pine tree, increased turpentine yield of the single pine tree and increased production efficiency are achieved.

Late embryogenesis abundant (Lea) proteins accumulate in maturing seeds after many of the storage compounds have been synthesized, and they are considered relevant to maturation. We report here the molecular organization and expression of BnLea3-1, a novel Group 3 Lea gene from Brassica napus. BnLea3-1 contains a coding region of 798 bp, sharing 84.4% homology at the amino acid level with Lea76 of B. napus. Two tandem 11-mer repeats are truncated from the coding region of BnLea3-1, compared to the 13 conserved 11-mer repeats of Lea76. Substitutions of consensus residues are found at various positions within the 11-mer repeats. A 1561 bp 5′ flanking promoter fragment of BnLea3-1 fused to E. coli-glucuronidase (GUS) coding region conferred seed-specific GUS expression in stable transgenics of B. napus, tobacco and in transiently-transformed pea. A −137 bp minimal promoter preceding the first transcription start site, identified through progressive deletions from the upstream was sufficient for basal GUS expression in the seeds and in leaves treated with ABA. Deletion studies indicate the presence of enhancing elements located between −137 bp to −742 bp and suppressing elements located between −742 and −1561 bp. BnLea3-1 expression in seeds precedes that of Lea76. Unlike other Group 3 Lea members including HVA1 and Dc3, BnLea3-1 is active in seeds and responsive weakly in vegetative tissues to ABA and methyl jasmonate (MeJA) but not to stress treatments. Possible functions of BnLea3-1 and another member of the Group 3 Lea family BnLea3-2 in embryo development is discussed.

The invention relates to a DCPTA-containing (2-(3,4-dichlorophenoxy)triethylamine containing) plant growth regulation composition, comprising active ingredient A and active ingredient B; the active ingredient A is DCPTA; the active ingredient B is selected from any one of brassinolide, gibberellic acid, 2-diethylaminoethyl hexanoate, indoleacetic acid, harpin Ea, jasmonic acid, methyl jasmonate, and allantoin; the DCPTA-containing plant growth regulation composition is mainly used for increasing activity of plant endonuclear, increasing the contents of chlorophyll, protein, nucleic acid, juice, oil and lipoid in plants, maintaining green and preventing aging, significantly enhancing photosynthetic effect in crops, promoting development of roots, promoting effective absorption of fertilizer by crops, increasing yield and income, assisting in good growth of subordinate parts of crops, enhancing water-fertilizer absorption by plants, increasing the accumulative storage of proteins, lipids and the like, and promoting cell division and growth.

The present invention relates to cotton tissue specific and pathogenic bacterium inducing promoter and their application, and belongs to the field of biotechnology. The promoter contains CAAT-box, TATA-box, ethylene, methyl jasmonate, abscisic acid response element, pathogenic bacterium inducer response elements W boxes, GT-1, MYB, MYBST1, MYB1LEPR, etc. There are also specific root expressing elements to enhance the expression of GUS gene, which expresses specifically in the phloem of root, bud and stem, the vein of foliage and the guard cell of air pore. Paraffin section observation shows that GHNBS promoter expresses in phloem companion cell. After treatment with SA, MeJA, Ethylene, pathogenic bacteria of blight and pseudomonas syringae DC3000, GUS will have obviously raised activity, so that it is one organ specific and pathogenic bacterium relevant promoter.

The invention relates to a plant growth adjusting agent, in particular to the application of jasmonic acid methyl ester analogue serving as the plant growth adjusting agent. The analogue serving as the plant growth adjusting agent has high biological activity, and low-density water solution can promote growth and sprouting of the plants. In addition, the adjusting anent promotes resistance of the plants, such as insect pest resistance, high temperature resistance, low temperature resistance, salt and alkali resistance, drought resistance, cold damage resistance and the like.

The invention relates to a plant growth regulating composition containing jasmonic acid and methyl jasmonate. The plant growth regulating composition contains an active component A and an active component B, wherein the active component A is jasmonic acid or methyl jasmonate, and the active component B is any one selected from a group consisting of benzyl aminopurine, forchlorfenuron, ethephon, tetramethyl glutarate, mepiquat chloride and choline chloride. The plant growth regulating composition is mainly used for improving enzyme activity, promoting seed germination, enhancing germination capacity and photosynthetic intensity, increasing the content of chlorophyll, maintaining greenness, preventing ageing, promoting development of roots and effective fertilizer absorption of crops, assisting the inferior part of a crop in good growth, enhancing absorption of water and fertilizer by plants and adjusting the balance of moisture in plants so as to improve drought resistance and cold resistance of plants; and the plant growth regulating composition can improve immunity, disease and insect resistance and crop quality and increase yield and income.

The invention belongs to the technical field of biological culture and particularly relates to a method for improving artemisinine content in sweet wormwood herb by methyl jasmonate treatment. The method comprises the following steps: firstly, planting seeds of the sweet wormwood herb in a sprouting culture medium for sprouting; secondly, transplanting sprouts of the sweet wormwood herb into a peat pearlstone culture medium, and then transplanting the strains of the sweet wormwood herb into the field for planting; and finally, spraying the strains of the sweet wormwood herb by using methyl jasmonat three to five days before harvesting. The method can effectively improve the artemisinine content in the sweet wormwood herb, has simple operation, does not need relatively higher early investment required in the process of improving the artemisinine content by utilizing a genetic engineering method, effectively reduces the production cost of the artemisinine and has great significance for meeting the large-scale production of the artemisinine.

The invention discloses application of MeJA on aspects of vegetable preservation. MeJA can be applied before sowing of vegetable seeds for soaking process. The invention also provides a vegetable preservation soaking solution, which is the solution of MeJA, with concentration 0.001 mu mol.L<-1> to 1000 mu mol.L<-1>. In the invention, aqueous solution of MeJA in a very low concentration is used to soak seeds of cabbages, and the seeds are used to sow to produce the cabbages, thereby increasing soluble solids and content of vitamin C of the cabbages, increasing freshness of the cabbages, relieving disease rotting rate of the cabbages after being picked, proloning preserving life of the cabbages. Quality of the vegetable using the technology of the invention is higher than that produced by a common method, thereby relieving long time insect pest in a certain extent, reducing consumption of the pesticide.

The invention discloses a method for promoting coloring of red Chinese pears by utilizing chemical agents. The method includes immersing uncolored Chinese pears which reach a physiological maturity stage into a coloring accelerator solution for 1-5 minutes, taking out the Chinese pears and drying the Chinese pears in the air, and placing the Chinese pears in an environment with a temperature of 12-22 DEG C, 70-90% of relative humidity, 0.1-0.5 micromole*m<-2>*s<-1> of luminous intensity of ultraviolet-b radiation (UV-B), 250-550 micromoles*m<-2>*s<-1> of luminous intensity of white light for 5-10 days, wherein the coloring accelerator comprises one or more kinds of ethephon, genistein and methyl jasmonate. According to the method, anthocyanin accumulation of the read Chinese pears can be effectively induced, the exterior quality of the pears can be improved, and the method and the coloring effect are better compared with the prior art after the immersing process of the red Chinese pears into the chemical agents.

The present invention relates to compositions and methods for treating cancer, by administering a combination comprising a jasmonate derivative (e.g., methyl jasmonate or a compound of any of formulae I through VII or any of the jasmonate derivatives exemplified by such formulae) and at least one other agent selected from a chemotherapeutic agent (e.g., a nitroso-urea, a platinum compound, a taxane derivative, an antitumor antibiotic) and an inhibitor of glycolysis (e.g., 2-deoxy-D-glucose). The jasmonate derivative and the at least one other agent together provide a therapeutic effect, which is preferably synergistic (cooperative).

The invention relates to a formula of a rooting agent, in particular to a rooting agent applicable to cutting seedling raising of green branches of camellia nitidissima. The rooting agent applicable to cutting seedling raising of the green branches of the camellia nitidissima is characterized in that the rooting agent is prepared from raw materials in parts by weight as follows: 3-10 parts of naphthalene acetamide, 10-15 parts of 3-indolebutyric acid, 10-15 parts of naphthylacetic acid, 3-10 parts of a pinellia ternate extracting solution, 3-10 parts of a chrysanthemum extracting solution, 5-8 parts of glycine, 5-8 parts of glutamic acid, 3-8 parts of methyl jasmonate, 3-10 parts of coronatine, 3-12 parts of vitamin B2, 3-12 parts of vitamin B6, 3-12 parts of vitamin B12, 3-7 parts of vitamin C and 2-7 parts of vitamin H. The rooting effect of the camellia nitidissima is effectively improved, the pest and disease damage resistance is improved, and root nutrition of the camellia nitidissima is effectively supplemented.

The invention relates to a growth regulator for landscape plant. The growth regulator is prepared from the following raw materials in parts by weight: 3-9 parts of potassium chloride, 2-6 parts of dipotassium phosphate, 4-12 parts of urea, 2-3 parts of methyl jasmonate, 1-2 parts of mixed humic acid, 1-2 parts of calcium superphosphate, 3-6 parts of lime, 3-6 parts of dicyandiamide, 0.3-0.5 part of ammonium sulfate, 0.3-0.5 part of potassium nitrate, 0.1-0.3 part of indoleacetic acid, 0.01-1 part of cytokinin, 10-20 parts of sodium nitrophenolate, 2-3 parts of biochemical sodium fulvate, 5-10parts of polyaspartic acid, 2-4 parts of sodium fulvate, 5-7 parts of rooting powder, 1-3 parts of gibberellin, 0.5-1 part of sophocarpidine, 3-6 parts of eugenol, 10-12 parts of starch, 0.02-0.06 part of abscisic acid, 2-3 parts of mefluidide, 3-5 parts of trinexapac-ethyl, 3-5 parts of diethylaminoethyl hexanoate, 3-5 parts of brassinolide, 0.1-0.3 part of forchlorfenuron, 0.5-0.7 part of traceelements, 20-30 parts of propylene glycol and 50-70 parts of water. The growth regulator for landscape plant is capable of increasing the chlorophyllous content of the landscape plant, is capable of promoting the plant growth, is capable of resisting against lodging and has an excellent effect for preventing pest and disease damage.

The invention discloses a phthorimaea operculella zell attractant comprising the following components in parts by volume: 8-10 parts of alcohol compound, 0.5-1.5 parts of ester compound, 2-3 parts of aldehyde compound, 1-3 parts of terpene compound and 0.1-0.5 part of other compound, wherein the alcohol compound is any one of 1,8-eucalyptol and palustrol; the ester compound is any one of methyl jasmonate, anti-4, cis-7-C13 diene acetate and anti-4, cis-7, 10-C13 triene acetate; the aldehyde compound is any one of heptaldehyde, octyl aldehyde and nonaldehyde; the terpene compound is any one of limonene, phellandrene, terpinene, caryophyllene, pinene, elemene, ocimene, germacrene, farnesene and isolongifolene; and the other compound is any one of nicotine, solanone, norsolandione and para-cymene. The attractant is environment-friendly, does not injure natural enemies of pests and has remarkable attracting effect on phthorimaea operculella zell adults.

The invention provides a method for strengthening the abiotic environmental stress resistance of crops. The method comprises the steps of putting seedlings in a simulation pool of abiotic environmental stress; uniformly sprinkling a plant growth regulator solution containing 1-aminocyclopropane-1-carboxyl and methyl jasmonate at the same time onto the leaves of the seedlings, wherein the sprinkling amount is as the criterion when all the leaves are completely wetted by the growth regulation solution. The method is a growth regulation mode capable of obviously increasing the accumulation of nitric nitrogen at the roots of the crops, so that the abiotic environmental stress resistance of the crops is obviously improved, and the abiotic environmental stress resistance of the crops for heavy metal contamination and salt stress in a growing environment is strengthened.

The invention discloses a pseudo-ginseng inducible promoter R1 and application thereof, the nucleotide sequence of R1 is shown as SEQ ID NO: 1, and molecular biology and genetic engineering related technical researches prove that the pseudo-ginseng promoter R1 responds to several plant hormones, biological stress and abiotic stress; a fusion expression cassette constructed by the panax notoginseng promoter R1 and the beta-glucuronidase gene is transferred into tobacco for expression, and the activity of the glucuronidase of the transgenic tobacco is quantitatively detected through a fluorescence method. The result shows that after the transgenic tobacco is treated by fusarium oxysporum, fusarium solani, alternaria alternata, NaCl, AlCl3, gibberellin, methyl jasmonate, salicylic acid, abscisic acid and ethephon, the activity of glucuronidase is obviously enhanced; the pseudo-ginseng promoter R1 is induced by several plant hormones and biological and abiotic stress factors, and can be used for plant disease-resistant gene engineering.

The invention discloses a detection method of jasmonic acid (JA) and methyl jasmonate (Me Ja) in a trace plant fresh sample on the basis of solid phase extraction and a tandem mass spectrum, comprising the following steps: (1) preparing a reverse carbon eighteen solid phase extraction small column purified and extracted by JA and MeJA; 2) extracting and purifying JA and MeJA in the plant fresh sample with a solid phase extraction method; and (3) sample detection: after the plant sample is purified and extracted, the content of JA and MeJA is detected by LCMS-MS (Liquid Chromatography Mass Spectrometry-Mass Spectrometry). The invention has the effect that the reverse carbon eighteen solid phase extraction small column is suitable for separating and purifying JA and MeJA, and JA and MeJA are simultaneously purified and separated; and meanwhile, JA and MeJA in a complex sample are separated and detected within the shorter time on the basis of the high sensitivity of the tandem mass spectrum to obtain the same result or result better than the prior detection method. The detection method has the advantages of simple result, low sample demand quantity and the like and is convenient to operate so as to detect JA and MeJA in the trace plant fresh sample at high sensitivity.