134 results about "Pyridoxal" patented technology

A composite auxin for making the flowering phase of kaffir lily earlier contains gibberellin, auxin, cytokinin, inositol, pyridoxal and acetylcholine.

A serum-free vegetable protein peptide general cell culture medium relates to a serum-free or low-serum general cell culture medium. The serum-free vegetable protein peptide general cell culture medium solves the problems that the existing serum-free culture medium can not directly culture the cells with adverse growth status, most of cells can be adaptable to the existing serum-free culture medium after the gradual reduction of the serum concentration, and the existing serum-free culture medium is vulnerable to the external factors and easy to cause fatal damage. The serum-free vegetable protein peptide general cell culture medium is prepared by calcium chloride, potassium chloride, magnesium sulfate anhydrous, sodium chloride, anhydrous sodium dihydrogen phosphate, L-glutamine, D-glucose, phenol red, D-calcium pantothenate, choline chloride, folic acid, i-inositol, nicotinamide, pyridoxal hydrochloride, riboflavin, thiamine hydrochloride, soybean protein peptide and distilled water. Cells can be directly cultured on the culture medium of the invention without gradual habituation and culture, thus shortening culture period and avoiding the interference by other factors, reducing the virus pollution which is caused by serum; the cell survival rate reaches more than 94 percent.

The invention relates to the technical field of enzyme catalysis, and specifically relates to a transaminase catalyst; and the invention further relates to a method for synthesizing (R)-1-tert-butoxycarbonyl-3-aminopiperidine by adopting an enzymatic way, as well as a production method of the (R)-1-tert-butoxycarbonyl-3-aminopiperidine. The method for synthesizing the (R)-1-tert-butoxycarbonyl-3-aminopiperidine by adopting the enzymatic way comprises a step of, in the presence of pyridoxal phosphate and the transaminase catalyst, allowing reaction of N-tert-butoxycarbonyl-3-piperidinone as a reaction substrate with an amino donor so as to produce the (R)-1-tert-butoxycarbonyl-3-aminopiperidine. The method for synthesizing the (R)-1-tert-butoxycarbonyl-3-aminopiperidine by adopting the enzymatic way utilizes relatively few reagents, and is mild in reaction conditions, so that tedious steps needed for chemical process synthesis are greatly simplified; and moreover, a target product withan ee value up to 99.77% or above can be obtained without performance of separation. Therefore, the transaminase catalyst and the process method utilizing the transaminase catalyst to synthesize the (R)-1-tert-butoxycarbonyl-3-aminopiperidine provided by the invention have broad application prospects as well as great market values.

The invention relates to an immobilized metal ion affinity chromatography (IMAC) functionalized hybrid monolithic material with a high specific surface area, prepared through a sol-gel technology, andan application thereof in phosphorylated peptide enrichment. A hybrid monolithic material containing phosphate functional groups on the surface is prepared through a sol-gel reaction by taking tetraethoxysilane (TEOS) and diethylphosphatoethyltriethoxysilane (DPTS) as precursors, the hybrid monolithic material is hydrolyzed in concentrated hydrochloric acid (HCl) to generate phosphoric acid functional groups on the surface, and the hydrolyzed hybrid monolithic material is chelated with Ti<4+> to obtain the Ti<4+>-IMAC hybrid monolithic material. The Ti<4+>-IMAC hybrid monolithic material canadsorb micromolecular pyridoxal 5-phosphate under the action of metal chelation, and can also be used for carrying out high-sensitivity and high-selectivity enrichment on phosphorylated peptide fragments in beta-casein and human cervical carcinoma cell (HeLa) enzymatic hydrolysate.

The present invention provides pharmaceutical compositions comprising a platelet aggregation inhibitor and a compound selected from pyridoxal-5'-phosphate, a pyridoxal-5'-phosphate related compound or a pharmaceutically acceptable salt thereof and methods for using a platelet aggregation inhibitor and a compound selected from pyridoxal-5'-phosphate, a pyridoxal-5'-phosphate related compound or a pharmaceutically acceptable salt thereof, for inhibiting platelet aggregation, for treating diseases that arise from prothrombotic and thrombotic states in which the coagulation cascade is activated and for reducing the risk of cardiovascular disease or cerebrovascular disease.

The invention discloses a glutamic acid decarboxylase recombinant bacterium, which is an Escherichia coli introduced with L-glutamic acid decarboxylase GAD; and the L-glutamic acid decarboxylase GAD gene is from yeast Kodamaea ohmeri NH-9 and has a nucleotide sequence shown as a SEQ ID No:1. The invention also discloses the construction method and application of the above glutamic acid decarboxylase recombinant bacterium. The invention has the following advantages: (1) hydrochloric acid pyridoxal has a price half of that of 5 ' -phosphoric acid pyridoxal, so as to reduce technology cost; (2) the method has high enzyme activity, short reaction time, mild conditions, substrate concentration up to 510 g / L and concentration of a product gamma-aminobutyric acid up to 352 g / L; (3) the bacteria and the buffer after rotary evaporation can be reused, and the conversion rate is 100%, and the yield is no less than 99%; and (4) the technology is simple, green and can realize a product purity higher than 99%.

The invention discloses a method for rapidly detecting the content of B vitamins in pig intestinal contents / excrement. The method comprises the following steps: weighing cecum contents / excrement of pigs as a sample, adding a methanol / acetonitrile / water solution, and performing homogeneous extraction to obtain a sample solution; and performing UPLC-MS / MS-MRM detection analysis on the sample solution to finally obtain the content of eight B vitamins in the sample, wherein the eight B vitamins are vitamin B1, vitamin B2, nicotinic acid, nicotinamide, pyridoxal, pyridoxine, vitamin B9 and vitaminB12. According to the method, an MRM technology is utilized, an ultra-high performance liquid chromatography system and a mass spectrometer are combined, and the content of a plurality of B vitamins in the samples such as the pig intestinal contents and the excrement can be rapidly detected based on targeted metabonomics of an MRM method.